一种适用于‘中草28号’老芒麦幼穗的高效组织培养与快速繁殖的方法

Through systematic hormone ratio experiments and a clear operating procedure, an efficient tissue culture and rapid propagation method for 'Zhongcao 28' wheat was established, solving the problems of genotype dependence and parameter loss, and realizing a highly efficient and reproducible technical solution that is suitable for molecular breeding and seed industry promotion.

CN121773953BActive Publication Date: 2026-07-17INSTITUTE OF GRASSLAND RESEARCH OF CAAS
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INSTITUTE OF GRASSLAND RESEARCH OF CAAS
Filing Date
2025-12-17
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing technologies lack a complete and efficient tissue culture and rapid propagation scheme for 'Zhongcao 28' old wheat, resulting in genotype dependence, missing key culture parameters, and a lack of systematic operating procedures, leading to unstable and unreproducible regeneration systems.

Method used

A systematic two-factor, four-level hormone ratio experiment was conducted to determine the optimal hormone conditions for the young spikelets of 'Zhongcao 28'. A complete technical process was provided, from explant selection, disinfection, callus induction, differentiation culture to seedling hardening and transplanting, including MS medium and specific hormone combinations (such as 2,4-D and 6-BA, NAA), establishing an operable and efficient regeneration system.

Benefits of technology

The study achieved high callus induction and differentiation rates for 'Zhongcao 28' wheat, ensuring the stability and reproducibility of the regeneration system, filling a technological gap, providing a reliable technical foundation for subsequent molecular breeding, and reducing development costs and time.

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Abstract

本发明提供了一种适用于‘中草28号’老芒麦幼穗的高效组织培养与快速繁殖的方法，属于植物组织培养技术领域。该方法包括：将外植体接种于诱导培养基上诱导产生愈伤组织；随后转接至分化培养基中进行芽的分化；最后将分化苗移入生根培养基1 / 2 MS中诱导生根，完成完整植株的再生。本发明通过优化的外植体选择、消毒程序及特定的激素配比，显著提高了老芒麦幼穗愈伤组织的诱导率、分化率与幼苗成活率，建立了稳定、高效的离体再生体系，为‘中草28号’老芒麦的种质资源保存、遗传改良及工厂化育苗提供了可靠的技术支撑。
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Citation Information

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