A pathogen concentration sampling device and method of use

Abstract

This invention discloses a pathogen concentration and sampling device and its usage method, comprising a dual-rotor mechanism, including an independently rotatable upper and lower rotary disks. The upper rotary disk has an upper through-hole and several concentration holes for installing packing material arranged circumferentially at intervals, while the lower rotary disk has a lower through-hole and several collection holes for placing collection pipes; a compression mechanism, including a cylindrical part with an internal water conveying channel and a moving part, one end of the cylindrical part being located above the upper rotary disk and smaller than the diameter of the concentration holes, and the other end being connected to a sewage conveying structure via a telescopic pipe; and a sample delivery mechanism, located below the dual-rotor mechanism, including a sample delivery component that can be wound or vertically erected in an unwound state and housed by a winding structure, with a double rubber roller structure connected to the end of the sample delivery component away from the winding structure. This invention, based on a dual-rotor design, concentrates and delivers sewage, solving the limitations of manhole space on sewage sampling while improving operational efficiency.

Description

Technical Field

[0001] This invention relates to the field of wastewater treatment technology, and in particular to a pathogen concentration and sampling device and its usage method. Background Technology

[0002] Sewer pipes are a crucial part of urban drainage systems. Urban domestic sewage is generally discharged, collected, and transported to sewage treatment plants through these pipes. Manholes are typically used at bends and branches in drainage pipes to facilitate unblocking and sampling of sewage. In particular, to improve my country's ability to detect infectious diseases, pathogenic microorganisms in urban sewage are commonly targeted for testing. Currently, when testing for pathogenic microorganisms in urban sewage through manholes, sampling personnel typically need to collect samples over several consecutive days. Each time, the manhole cover must be opened before sampling, a time-consuming and labor-intensive process that reduces efficiency. Furthermore, to ensure a high concentration of pathogenic microorganisms in the obtained samples, large-scale sewage sampling is required, which is challenging due to the limited space within the manhole.

[0003] In view of this, it is necessary to design a pathogen concentration sampling device and its usage method to solve the above problems. Summary of the Invention

[0004] The purpose of this invention is to provide a pathogen concentration and sampling device and its usage method. Based on a dual-rotor design, it concentrates and delivers wastewater samples, that is, it concentrates the virus below the manhole before delivering a small amount of sample, which solves the limitation of manhole space on wastewater sampling and improves operational efficiency.

[0005] To achieve the above-mentioned objectives, the present invention provides a pathogen concentration and sampling device, comprising: The dual-rotor mechanism includes an upper rotary disk and a lower rotary disk that can rotate independently. The upper rotary disk is provided with upper through holes and several concentration holes for installing packing in a circumferentially spaced manner. The lower rotary disk is provided with lower through holes and several collection holes for placing collection pipes in a corresponding circumferentially spaced manner. The extrusion mechanism includes a cylindrical section with an internal water conveying channel and a moving section for controlling the vertical reciprocating movement of the cylindrical section. One end of the cylindrical section is located above the upper turntable and is smaller than the diameter of the concentration hole, while the other end is connected to a wastewater conveying structure via a telescopic pipe. The sample feeding mechanism is located below the double turntable mechanism. It includes a sample feeding component that can be wound and can stand upright in a non-wound state and is housed by a winding structure. The end of the sample feeding component away from the winding structure is connected to a double rubber roller structure, so that under the action of the double rubber roller structure, the end of the sample feeding component away from the winding structure can move upward to pass through the collection hole and the upper through hole opposite to the collection hole for collection tube transportation. When the two turntables rotate to the position where the concentration hole and the lower through hole are directly below the column, it is in the concentration position; when the concentration hole and the collection hole are directly below the column, it is in the collection position; and when the collection hole and the upper through hole are directly above the sample feeder, it is in the transport position.

[0006] As a further improvement of the present invention, the filler is a porous material that can absorb water.

[0007] As a further improvement of the present invention, the diameters of the collection hole and the upper through hole are both larger than the maximum diameter of the sample delivery piece.

[0008] As a further improvement of the present invention, the water supply channels at intervals are provided at one end of the column near the upper turntable, so that a water supply area and a squeezing area are formed on the cross-section of the end of the column.

[0009] As a further improvement of the present invention, the end of the sample feeder away from the winding structure is provided with a support portion adapted to the collection tube.

[0010] As a further improvement of the present invention, the sample is heat-treated spring steel.

[0011] As a further improvement of the present invention, the dual rubber roller structure is located above the output end of the winding structure.

[0012] As a further improvement of the present invention, when the two turntables rotate to the concentration position, a waste liquid pipe is provided below the lower through hole to discharge the waste liquid of the concentration process to a predetermined position.

[0013] As a further improvement of the present invention, a methane sensor for detecting the methane concentration in a manhole is also included.

[0014] The present invention also provides a method for using the pathogen concentration sampling device, comprising the following steps: S1. Control the two turntables to rotate until the thickening hole and the lower through hole are both directly below the column body, start the sewage conveying structure, and convey the sewage to be thickened through the water conveying channel to the thickening hole, so that the pathogens in the sewage to be thickened are captured by the packing, and the treated waste liquid flows out through the lower through hole. S2. Control the lower turntable to rotate until the collection hole containing the collection tube is directly below the column body. Control the column body to move down through the moving part to squeeze the packing in the concentration hole, so that the pathogens are separated from the packing and flow to the collection tube. S3. Control the two turntables to rotate until the collection tube containing pathogens and the upper through hole are directly above the sample delivery component. Control the sample delivery component to move upwards through the winding structure and double rubber roller structure until it merges with the collection tube clamp, passes through the collection hole and the upper through hole in sequence, and continues to move upwards until the collection tube is delivered to the manhole.

[0015] The beneficial effects of this invention are: 1. This invention, by setting up a dual-rotor mechanism, can perform alignment and conversion of different hole positions. First, the pathogen is concentrated through the cooperation of the dual-rotor mechanism and the extrusion mechanism, so that the weight of the concentrated sample is greatly reduced compared with the original sewage sample. This makes it easier to transport small-weight concentrated samples using the vertically upright sample feeder in the non-coiled state, solving the limitation of manhole space on sampling while improving work efficiency.

[0016] 2. The present invention is based on the setting of concentrating the sample before transporting it. It can be adapted to the specific structure of the sample delivery component, which can be both rolled up and stand upright in an unrolled state, resulting in limited load-bearing capacity. That is, by concentrating the sample, the weight of the sample is controlled within the load-bearing range of the sample delivery component, which can avoid the situation where the sample cannot be transported to the target height due to bending of the sample delivery component during the sample transport process.

[0017] 3. The present invention utilizes a double-roller structure to control the vertical movement of the sample feeder. This structure can utilize the frictional force generated by the reverse movement of the two rollers to drive the sample feeder to unfold and move vertically. It can also utilize the clamping of the sample feeder by the two rollers to fix the lower part of the sample feeder, thereby improving the stability of the sample feeder in the sample feeding state to a certain extent.

[0018] 4. The dual-rotor structure of the present invention can, on the one hand, cooperate with the extrusion mechanism and the sample delivery mechanism to concentrate and deliver wastewater; on the other hand, during the sample delivery process, when the sample delivery part passes through the collection hole and the upper through hole, the collection hole and the upper through hole can also be used to limit the sample delivery part. Thus, under the fixing effect of the dual rubber roller structure and the limiting effect of the collection hole and the upper through hole, the lifting stability of the sample delivery part is ensured, and the bending of the sample delivery part during long-distance sample delivery is avoided, which affects the sample transport.

[0019] 5. The sample feeding component of the present invention can be wound or set in a vertically upright state when not wound, which can increase the conveying height of the sample feeding mechanism while reducing the volume occupied by the sample feeding mechanism, so that the conveying height of the sample feeding mechanism can reach 0.5m.

[0020] 6. The dual-rotor mechanism of the present invention, with its multi-hole position switching capability, enables multiple samplings during a single material change, reducing operational difficulty while meeting the requirement for continuous multiple samplings. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of a pathogen concentration and sampling device.

[0022] Figure 2 This is a schematic diagram of the internal structure of a pathogen concentration and sampling device.

[0023] Figure 3 This is a schematic diagram of the rotation control of the dual-turntable mechanism.

[0024] Figure 4This is a schematic diagram of the dual-rotor mechanism in the condensation position.

[0025] Figure 5 This is a schematic diagram of the dual-rotor mechanism in the collection position.

[0026] Figure 6 This is a schematic diagram of the dual-turntable mechanism in the conveying position.

[0027] Figure 7 This is a schematic diagram of the cross-section of the first cylinder.

[0028] Figure 8 This is a schematic diagram of the extrusion mechanism connection.

[0029] Figure 9 This is a schematic diagram of the sample delivery mechanism.

[0030] Figure Labels 10. Housing; 11. Window; 12. Horizontal reciprocating moving mechanism; 13. Cover plate; 14. Methane sensor; 15. Internal liquid level sensor; 20. Double turntable mechanism; 21. Upper turntable; 211. Upper through hole; 212. Concentration hole; 22. Lower turntable; 221. Lower through hole; 222. Collection hole; 231. Cylinder; 232. Gear ring; 233. Gear; 234. First motor; 241. Drive shaft; 242. Second motor; 31. Column part; 311. First cylinder; 3111. Water conveying area; 3112. Squeezing area; 312. Horizontal column; 313. Second cylinder; 314. Telescopic pipe; 32. Moving part; 321. Fixing part; 322. Moving part; 41. Winding structure; 42. Sample feeding part; 421. Support part; 43. Double rubber roller structure; 50. Collection pipe. Detailed Implementation

[0031] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be described in detail below with reference to the accompanying drawings and specific embodiments.

[0032] It should also be noted that, in order to avoid obscuring the present invention with unnecessary details, only the structures and / or processing steps closely related to the present invention are shown in the accompanying drawings, while other details that are not closely related to the present invention are omitted.

[0033] Additionally, it should be noted that the terms “comprising,” “including,” or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0034] like Figures 1-9As shown, the present invention provides a pathogen concentration sampling device, characterized in that it comprises: The dual-turntable mechanism 20 includes an upper turntable 21 and a lower turntable 22 that can rotate independently. The upper turntable 21 is provided with an upper through hole 211 and a plurality of concentration holes 212 for installing packing in the circumferential direction. The lower turntable 22 is provided with a lower through hole 221 and a plurality of collection holes 222 for placing the collection tube 50 in the corresponding circumferential direction. The extrusion mechanism includes a cylindrical section 31 with an internal water conveying channel and a moving section 32 for controlling the vertical reciprocating movement of the cylindrical section 31. One end of the cylindrical section 31 is located above the upper turntable 21 and is smaller than the diameter of the concentration hole 212; the other end is connected to a sewage conveying structure via a telescopic pipe 314. The sample feeding mechanism is located below the double turntable mechanism 20. It includes a sample feeding component 42 that can be wound and can stand upright in a non-wound state and is housed by the winding structure 41. The end of the sample feeding component 42 away from the winding structure 41 is connected to a double rubber roller structure 43, so that under the action of the double rubber roller structure 43, the end of the sample feeding component 42 away from the winding structure 41 can move upward to pass through the collection hole 222 and the upper through hole 211 opposite to the collection hole 222 for collection tube 50 conveying. When the two turntables rotate to the position where the concentration hole 212 and the lower through hole 221 are directly below the column part 31, they are in the concentration position; when the concentration hole 212 and the collection hole 222 are directly below the column part 31, they are in the collection position; and when the collection hole 222 and the upper through hole 211, which have completed collection, are directly above the sample delivery piece 42, they are in the delivery position.

[0035] For example, the pathogen concentration sampling device also includes a housing 10. A dual-rotor mechanism 20, a squeezing mechanism, and a sample delivery mechanism are all disposed within the inner cavity of the housing 10. A window 11 for the sample delivery component 42 to extend from the top of the housing 10 is provided. A cover plate 13, controlled by a horizontal reciprocating movement mechanism 12, is provided at the window 11. When sample delivery is required, the cover plate 13 is moved open by the horizontal reciprocating movement mechanism 12 to expose the window 11. When sample delivery is not required, the sliding cover plate 13 is moved to cover the window 11 to close it. In this example, a track connected to the cover plate 13 is provided around the window 11 to allow the cover plate 13 to slide. The horizontal reciprocating movement mechanism 12 can be any existing reciprocating movement mechanism that enables the reciprocating movement of the cover plate 13, such as an electric push rod. The specific structure of the horizontal reciprocating movement mechanism 12 will not be described in detail here.

[0036] An inner liquid level sensor 15 and an outer liquid level sensor are respectively installed on the inner and outer walls of the bottom of the housing 10. When the pathogen concentration sampling device is placed in the manhole, the outer liquid level sensor monitors whether sewage has spread to the pathogen concentration sampling device, and the inner liquid level sensor 15 monitors whether sewage has leaked into the housing 10, so as to ensure the safety of the pathogen concentration sampling device.

[0037] The top of the housing 10 is also equipped with a methane sensor 14 for monitoring the methane concentration in the manhole.

[0038] For example, such as Figure 3 As shown, a hollow cylinder 231 is provided in the middle of the lower turntable 22, and a gear ring 232 connected to the first motor 234 via a gear 233 is arranged around the outer circumference of the cylinder 231; a drive shaft 241 is provided in the middle of the upper turntable 21, and the drive shaft 241 passes through the hollow part of the cylinder 231 and is connected to the second motor 242. The rotation of the lower turntable 22 and the upper turntable 21 are controlled by the first motor 234 and the second motor 242, respectively.

[0039] For example, the packing material is a water-absorbing porous material. In this example, the porous material is made of polyester fiber. During the concentration process, the water absorption and porous properties of the porous material are used to capture pathogens from flowing wastewater. Then, under the extrusion pressure of the column section 31, the pathogens are absorbed and concentrated. The pore size of the packing material is set according to the target pathogen, ensuring that the packing material can be used for pathogen concentration.

[0040] For example, the diameters of the collection hole 222 and the upper through hole 211 are both slightly larger than the maximum diameter of the sample feeder 42, so that while the sample feeder 42 is used for sample transport, the collection hole 222 and the upper through hole 211 can also be used to limit the sample feeder 42, thereby improving the stability of sample delivery.

[0041] For example, such as Figure 8 As shown, the column part 31 includes a first cylinder 311, a horizontal column 312, and a second cylinder 313 connected in sequence. The first cylinder 311 is provided with a plurality of water conveying channels parallel to its axial direction, so that a water conveying area 3111 and a squeezing area 3112 are formed on the cross section of the first cylinder 311. Figure 7 The horizontal column 312 has a first channel connected to the water conveyance channel inside, and the second column 313 has a second channel connected to the first channel inside.

[0042] The lower part of the second cylinder 313 is connected to the sewage conveying structure via a telescopic pipe 314.

[0043] The movable part 32 includes a fixing member 321 fixed to the bottom of the housing 10 and a movable member 322 that can move up and down relative to the fixing member 321. The movable member 322 is fixedly connected to the second cylinder 313. Under the action of driving force, when the movable member 322 moves relative to the fixing member 321, it drives the second cylinder 313 to move up and down, which in turn drives the first cylinder 311 to move up and down, thereby extruding the packing in the concentration hole 212.

[0044] For example, the sewage transport structure includes a water delivery pipe connected to the telescopic conduit 314 and a water delivery pump.

[0045] The housing 10 is provided with holes for the water supply pipe to extend outside the housing 10.

[0046] For example, the sample feeder 42 and the winding structure 41 are the same as those in the prior art measuring tape. The sample feeder 42 is made of heat-treated spring steel and can be wound on the winding structure 41. When not wound on the winding structure 41, it is vertically upright.

[0047] The sample delivery part 42 is provided with a support part 421 adapted to the collection tube 50 at the end away from the winding structure 41. Figure 6 For example, the collecting tube 50 is a cylindrical tube, and the support portion 421 has an inwardly recessed cavity that is larger than the diameter of the cylindrical tube.

[0048] For example, such as Figure 9 As shown, the dual-roller structure 43 is located above the output end of the winding structure 41. The dual-roller structure 43 includes two rollers arranged side by side that rotate in opposite directions under a driving force, and the sample feeder 42 passes through the gap between the two rollers.

[0049] For example, when the two turntables rotate to the concentration position, a waste liquid pipe is provided below the lower through hole 221 to discharge the waste liquid of the concentration process to a predetermined position. In this example, the housing 10 is provided with an opening for the waste liquid pipe to extend outside the housing 10, and the opening and the hole for the water pipe to extend outside the housing 10 are symmetrically arranged on both sides of the housing 10.

[0050] The present invention also provides a method for using a pathogen concentration sampling device, comprising the following steps: S1. Set up the sampling point in the manhole, place the shell 10 at the sampling point, and extend the water pipe to connect with the sewage; S2. During sampling, control the two turntables to rotate until both the concentration hole 212 and the lower through hole 221 are directly below the first cylinder 311. Figure 4 The sewage conveying structure is activated, and the sewage to be concentrated flows sequentially through the water conveying pipe, the second cylinder 313, the horizontal column 312 and the first cylinder 311 to the concentration hole 212, so that the pathogens in the sewage to be concentrated are captured by the packing material, and the treated waste liquid flows out through the lower through hole 221. S3. Then, control the lower turntable 22 to rotate until the collection hole 222, where the collection tube 50 is placed, is directly below the first cylinder 311. Control the moving part 32 to move the first cylinder 311 down until the first cylinder 311 extends into the concentration hole 212, squeezing the packing material in the concentration hole 212, so that the pathogens are separated from the packing material and flow into the collection tube 50. Figure 5 ), to obtain a concentrated pathogen solution; S4. The cover plate 13 is moved by the horizontal reciprocating moving mechanism 12 until the window 11 is exposed; the two turntables are rotated until the collection tube 50 containing the pathogen concentrate and the upper through hole 211 are directly above the sample delivery component 42; the sample delivery component 42 is moved upward by the winding structure 41 and the double rubber roller structure 43 until it is engaged with the collection tube 50 and passes through the collection hole 222, the upper through hole 211 and the window 11 in sequence, and continues to move upward until the collection tube 50 is delivered to the top of the manhole. Figure 6 ), after the sampling personnel take away the collection tube 50.

[0051] After the current sampling is completed, the sample feeder 42 is returned to its initial state by the winding structure 41 and the double rubber roller structure 43, and the cover plate 13 is moved to the closed window 11 by the horizontal reciprocating moving mechanism 12.

[0052] For example, the volume of the pathogen concentrate is 1-5 mL.

[0053] During the next sampling, pathogen concentration is achieved through the packing material in another concentration hole 212. By placing packing material in multiple concentration holes 212 on the upper rotary table 21, concentration can be performed using different concentration holes 212 during continuous sampling, eliminating the need for frequent manual packing changes. Once all the packing material in all concentration holes 212 on the upper rotary table 21 is used up, the pathogen concentration sampling equipment is removed from the manhole for packing replacement, enabling multiple sampling with a single material change and effectively improving operational efficiency.

[0054] For example, the control of each part of the pathogen concentration sampling equipment during use can be adjusted by referring to the remote control method in the prior art so that remote control can be achieved when the pathogen concentration sampling equipment is placed in the manhole, which will not be elaborated here.

[0055] The above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. A pathogen concentration and sampling device, characterized in that, include: The dual-rotor mechanism includes an upper rotary disk and a lower rotary disk that can rotate independently. The upper rotary disk is provided with upper through holes and several concentration holes for installing packing in a circumferentially spaced manner. The lower rotary disk is provided with lower through holes and several collection holes for placing collection pipes in a corresponding circumferentially spaced manner. The extrusion mechanism includes a column part with a water conveying channel formed inside and a moving part for controlling the column part to move vertically back and forth. One end of the column part is located above the upper turntable and is smaller than the diameter of the concentration hole, and the other end is connected to a sewage conveying structure through a telescopic pipe. as well as The sample feeding mechanism, located below the dual-turntable mechanism, includes a sample feeding component housed by a winding structure, which can be wound or stand upright in a non-wound state. The end of the sample feeding component away from the winding structure is connected to a dual-roller structure, allowing the end of the sample feeding component away from the winding structure to move upward under the action of the dual-roller structure to pass through the collection hole and the upper through hole opposite to the collection hole for collection tube conveying. The end of the sample feeding component away from the winding structure is provided with a support part adapted to the collection tube. When the two turntables rotate to the position where the concentration hole and the lower through hole are directly below the column, it is in the concentration position; when the concentration hole and the collection hole are directly below the column, it is in the collection position; and when the collection hole and the upper through hole are directly above the sample feeder, it is in the transport position.

2. The pathogen concentration and sampling device according to claim 1, characterized in that: The filler is a porous material that can absorb water.

3. The pathogen concentration and sampling device according to claim 1, characterized in that: The diameters of the collection hole and the upper through hole are both larger than the maximum diameter of the sample.

4. The pathogen concentration and sampling device according to claim 1, characterized in that: The column section near the upper turntable has multiple water conveying channels spaced apart, forming a water conveying zone and a squeezing zone on the cross-section of the column section end.

5. The pathogen concentration and sampling device according to claim 1, characterized in that: The sample was made of heat-treated spring steel.

6. The pathogen concentration and sampling device according to claim 1, characterized in that: The dual-roller structure is located above the output end of the winding structure.

7. The pathogen concentration and sampling device according to claim 1, characterized in that: When the two turntables rotate to the concentration position, a waste liquid pipe is provided below the lower through hole to discharge the waste liquid of the concentration process to a predetermined position.

8. The pathogen concentration and sampling device according to claim 1, characterized in that: It also includes a methane sensor for detecting methane concentration in manholes.

9. A method of using the pathogen concentration sampling device according to claim 1, characterized in that, Includes the following steps: S1. Control the two turntables to rotate until the thickening hole and the lower through hole are both directly below the column body. Start the sewage conveying structure to transport the sewage to be thickened to the thickening hole through the water conveying channel, so that the pathogens in the sewage to be thickened are captured by the packing material, and the treated waste liquid flows out through the lower through hole. S2. Control the lower turntable to rotate until the collection hole containing the collection tube is directly below the column body. Control the column body to move down through the moving part to squeeze the packing in the concentration hole, so that the pathogens are separated from the packing and flow to the collection tube. S3. Control the two turntables to rotate until the collection tube containing pathogens and the upper through hole are directly above the sample delivery component. Control the sample delivery component to move upwards through the winding structure and double rubber roller structure until it merges with the collection tube clamp, passes through the collection hole and the upper through hole in sequence, and continues to move upwards until the collection tube is delivered to the manhole.

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