5α-还原酶突变体及其在甾体C4,5位加氢药物中间体合成中的应用
By mutating specific amino acid sequences of 5α-reductase, a highly efficient 5α-reductase mutant was constructed, solving the problems of low conversion rate and environmental hazards of steroid drug intermediates in existing technologies, and realizing efficient and green synthesis of steroid drug intermediates.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
- Filing Date
- 2026-03-04
- Publication Date
- 2026-07-17
AI Technical Summary
In existing technologies, the activity of 5α-reductase is low, resulting in low conversion and yield of steroidal drug intermediates. Furthermore, chemical and microbial fermentation methods pose environmental hazards and low efficiency.
By specifically mutating the amino acid sequence of 5α-reductase, especially modifying positions 56, 109, and/or 114, a highly efficient 5α-reductase mutant was constructed and combined with glucose-6-phosphate dehydrogenase to achieve efficient conversion at substrate concentrations up to 10 g/L.
It significantly improves the catalytic performance of 5α-reductase, especially achieving a 99% conversion rate for AD, shortening the conversion cycle, meeting the needs of industrial production, avoiding environmental hazards, and featuring a simple process suitable for large-scale production.
Smart Images
Figure CN121780465B_ABST