Plant growth regulator for prolonging the flowering period of garden flowers and its preparation method and application
By using plant growth regulators containing ingredients such as theaflavins, huperzine B, and Elaeagnus pungens leaf oil, the problem of premature senescence in flowering plants has been solved, thus extending the flowering period and improving the viewing period of garden flowers.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- SHANDONG FUBO INTELLIGENT CONTROL AGRICULTURE CO LTD
- Filing Date
- 2025-12-30
- Publication Date
- 2026-07-21
AI Technical Summary
Premature senescence during the flowering period of flowering plants causes the flower organs to wither rapidly, affecting pollination and ornamental value. Existing technologies are insufficient to effectively extend the flowering period.
This plant growth regulator, composed of theaflavins, huperzine B, Elaeagnus pungens leaf oil, salicylic acid, calcium chloride, and 6-benzylaminopurine, is applied via foliar spray to synergistically protect cell membranes and promote flower bud differentiation and flower development.
It significantly prolongs the flowering period of garden flowers, extends the viewing period, promotes earlier flowering and prolongs the flowering period, and enhances the physiological activities and ornamental effects of flowers.
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Abstract
Description
Technical Field
[0001] This invention belongs to the field of plant growth regulator technology, specifically relating to a plant growth regulator for extending the flowering period of garden flowers, its preparation method, and its application. Background Technology
[0002] Flowering plants go through a process from blooming to withering, signifying the formation, development, maturation, and senescence of their floral organs. During senescence, the plant's floral organs experience insufficient nutrient supply, a decline in physiological metabolism, and an inability of antioxidant enzymes and osmotic regulators to prevent tissue damage caused by reactive oxygen species—namely, cell membrane rupture and cytoplasmic destruction—ultimately leading to the end of the flowering period. Premature senescence causes the floral organs to wither and die rapidly, hindering pollination and reproduction, and significantly reducing their ornamental value, thus negatively impacting the overall landscape effect.
[0003] Plant floral organs undergo a process of formation, development, maturation, and senescence. Maintaining the physiological condition and nutritional levels of floral organs can effectively prolong the flowering period. The flowering period of a plant is influenced by its own genetic factors, as well as external environmental factors and human intervention. Plant growth regulators, including plant hormones and growth retardants, can regulate the growth, development, and nutrient distribution processes at various stages of plant growth. By utilizing plant characteristics to screen for the most suitable plant growth regulators, the growth, development, and physiological levels of floral organs can be effectively controlled, thereby extending the flowering period.
[0004] With the development of science and technology and the improvement of people's living standards, the concept of ecological construction has taken root in people's hearts. Flowering plants are widely used all over the world, from courtyards, communities, and parks to cities, from coastlines, wetlands, and plains to mountains. People's demand for flowering plant landscapes has never stopped. Inhibiting the senescence process of flowering plant flower organs, maintaining the flowering period, and ensuring that the scenery has a good ornamental effect for a certain period of time have become increasingly important scientific issues. Summary of the Invention
[0005] The purpose of this invention is to provide a plant growth regulator that significantly prolongs the flowering period of flowers in gardens and enhances the viewing period of garden flowers.
[0006] To achieve the above-mentioned technical objectives, the technical solution adopted by the present invention is as follows: A plant growth regulator for prolonging the flowering period of garden flowers, comprising the following raw materials: Theaflavins, huperzine B, Elaeagnus angustifolia leaf oil, salicylic acid, calcium chloride, 6-benzylaminopurine; The concentrations of theaflavins were 10-30 mg / L, huperzine B was 0.2-0.4 mg / L, Elaeagnus pungens leaf oil was 100-300 mg / L, salicylic acid was 5-10 mg / L, calcium chloride was 80-120 mg / L, and 6-benzylaminopurine was 4-7 mg / L.
[0007] Furthermore, the preparation method of the Elaeagnus pungens leaf oil is as follows: Take dried Elaeagnus pungens leaves, grind them into powder, add a complex hydrolytic enzyme solution of cellulase, hemicellulase and β-glucosidase, and enzymatically hydrolyze for 6 hours at 30-40℃. Then add ethanol and mix, and bathe in a water bath at 50℃ for 2 hours. Filter, and concentrate the filtrate by rotary evaporation to an oily state. Wash the oil phase with a small amount of distilled water to remove water-soluble impurities, and obtain Elaeagnus pungens leaf oil.
[0008] Furthermore, the mass-to-volume ratio of Elaeagnus pungens leaves, compound hydrolytic enzyme solution, and ethanol is 200g:1L:1L.
[0009] Furthermore, the complex hydrolase contains 12-15 g / L of cellulase, 5-8 g / L of hemicellulase, and 14-22 g / L of β-glucosidase.
[0010] Furthermore, the plant growth regulator is a liquid.
[0011] Furthermore, the plant growth regulator is applied by foliar spraying during the flowering period.
[0012] A method for preparing a plant growth regulator to prolong the flowering period of garden flowers includes the following steps: (1) Weigh out the required concentration of 6-benzylaminopurine and dissolve it in dimethyl sulfoxide to obtain a 6-benzylaminopurine solution. Then, weigh out theaflavin and huperzine B according to the required concentration and dissolve them in ethanol to obtain theaflavin solution and huperzine B solution respectively. (2) Mix Elaeagnus pungens oil and Tween-80 at a volume ratio of 1:2 to obtain an emulsion; (3) Take a dilution container, weigh calcium chloride and salicylic acid according to the required concentration, add water and stir until dissolved, then add the 6-benzylaminopurine solution, theaflavin solution and huperzine B solution from step (1) respectively, continue to add the emulsion from step (2), and finally add water to make up the volume and stir evenly to obtain the final product plant growth regulator.
[0013] Furthermore, in step (1), the amount of dimethyl sulfoxide used is just enough to dissolve 6-benzylaminopurine; the ratio of theaflavins to ethanol is 10 mg: 1 mL; and the ratio of huperzine B to ethanol is 0.1 mg: 1 mL.
[0014] This invention provides the application of the plant growth regulator in extending the flowering period of garden flowers.
[0015] Furthermore, the garden flowers mentioned are roses, magnolias, and crape myrtles.
[0016] All raw materials used in this invention are commercially available.
[0017] Beneficial effects This invention involves the extraction of components from Elaeagnus pungens leaves, revealing that Elaeagnus pungens leaf oil can effectively prolong the flowering period of ornamental flowers. Theaflavins are polyphenolic compounds produced during tea fermentation, possessing antioxidant and antibacterial properties. Huperzine B is an alkaloid extracted from plants in the Huperziaceae family. This invention innovatively introduces theaflavins, huperzine B, and Elaeagnus pungens leaf oil. These three components, combined with salicylic acid, calcium chloride, and 6-benzylaminopurine, form a plant growth regulator. The synergistic effect of these multiple substances protects cell membranes and petal tissues from oxidative damage, delaying petal wilting and discoloration, and helping flowers maintain physiological activities for a longer period. Furthermore, it promotes flower bud differentiation and flower development, accelerating flowering and extending the flowering period.
[0018] The plant growth regulator of this invention effectively promotes the early flowering of roses, crape myrtles, and magnolias in gardens, and prolongs the flowering period of flowers, thus extending the viewing period of garden flowers. Detailed Implementation
[0019] The technical solution of the present invention will be further described below with reference to specific embodiments, but it is not limited thereto.
[0020] Example 1 A plant growth regulator for prolonging the flowering period of garden flowers, comprising the following raw materials: Theaflavins, huperzine B, Elaeagnus angustifolia leaf oil, salicylic acid, calcium chloride, 6-benzylaminopurine; The concentrations of theaflavins were 10 mg / L, huperzine B was 0.2 mg / L, Elaeagnus pungens leaf oil was 100 mg / L, salicylic acid was 5 mg / L, calcium chloride was 80 mg / L, and 6-benzylaminopurine was 7 mg / L.
[0021] The preparation method of the Elaeagnus pungens leaf oil is as follows: Take dried Elaeagnus pungens leaves, grind them into powder, add a complex hydrolytic enzyme solution of cellulase, hemicellulase and β-glucosidase, and enzymatically hydrolyze for 6 hours at 30-40℃. Then add ethanol and mix, and bathe in a water bath at 50℃ for 2 hours. Filter, and concentrate the filtrate by rotary evaporation to an oily state. Wash the oil phase with a small amount of distilled water to remove water-soluble impurities, and obtain Elaeagnus pungens leaf oil.
[0022] The mass-to-volume ratio of Elaeagnus pungens leaves, compound hydrolytic enzyme solution, and ethanol is 200g:1L:1L.
[0023] The complex hydrolase contains 12 g / L cellulase, 5 g / L hemicellulase, and 14 g / L β-glucosidase.
[0024] A plant growth regulator for prolonging the flowering period of garden flowers is prepared by the following steps: (1) Weigh out the required concentration of 6-benzylaminopurine and dissolve it in dimethyl sulfoxide to obtain a 6-benzylaminopurine solution. Then, weigh out theaflavin and huperzine B according to the required concentration and dissolve them in ethanol to obtain theaflavin solution and huperzine B solution respectively. (2) Mix Elaeagnus pungens oil and Tween-80 at a volume ratio of 1:2 to obtain an emulsion; (3) Take a dilution container, weigh calcium chloride and salicylic acid according to the required concentration, add water and stir until dissolved, then add the 6-benzylaminopurine solution, theaflavin solution and huperzine B solution from step (1) respectively, continue to add the emulsion from step (2), and finally add water to make up the volume and stir evenly to obtain the final product plant growth regulator.
[0025] In step (1), the amount of dimethyl sulfoxide used is just enough to dissolve 6-benzylaminopurine; the ratio of theaflavins to ethanol is 10 mg: 1 mL; and the ratio of huperzine B to ethanol is 0.1 mg: 1 mL.
[0026] Example 2 A plant growth regulator for prolonging the flowering period of garden flowers, comprising the following raw materials: Theaflavins, huperzine B, Elaeagnus angustifolia leaf oil, salicylic acid, calcium chloride, 6-benzylaminopurine; The concentrations of theaflavins were 20 mg / L, huperzine B was 0.3 mg / L, Elaeagnus pungens leaf oil was 200 mg / L, salicylic acid was 7 mg / L, calcium chloride was 100 mg / L, and 6-benzylaminopurine was 6 mg / L.
[0027] The preparation method of the Elaeagnus pungens leaf oil is as follows: Take dried Elaeagnus pungens leaves, grind them into powder, add a complex hydrolytic enzyme solution of cellulase, hemicellulase and β-glucosidase, and enzymatically hydrolyze for 6 hours at 30-40℃. Then add ethanol and mix, and bathe in a water bath at 50℃ for 2 hours. Filter, and concentrate the filtrate by rotary evaporation to an oily state. Wash the oil phase with a small amount of distilled water to remove water-soluble impurities, and obtain Elaeagnus pungens leaf oil.
[0028] The mass-to-volume ratio of Elaeagnus pungens leaves, compound hydrolytic enzyme solution, and ethanol is 200g:1L:1L.
[0029] The complex hydrolase contains 14 g / L cellulase, 6 g / L hemicellulase, and 18 g / L β-glucosidase.
[0030] A plant growth regulator for prolonging the flowering period of garden flowers is prepared by the following steps: (1) Weigh out the required concentration of 6-benzylaminopurine and dissolve it in dimethyl sulfoxide to obtain a 6-benzylaminopurine solution. Then, weigh out theaflavin and huperzine B according to the required concentration and dissolve them in ethanol to obtain theaflavin solution and huperzine B solution respectively. (2) Mix Elaeagnus pungens oil and Tween-80 at a volume ratio of 1:2 to obtain an emulsion; (3) Take a dilution container, weigh calcium chloride and salicylic acid according to the required concentration, add water and stir until dissolved, then add the 6-benzylaminopurine solution, theaflavin solution and huperzine B solution from step (1) respectively, continue to add the emulsion from step (2), and finally add water to make up the volume and stir evenly to obtain the final product plant growth regulator.
[0031] In step (1), the amount of dimethyl sulfoxide used is just enough to dissolve 6-benzylaminopurine; the ratio of theaflavins to ethanol is 10 mg: 1 mL; and the ratio of huperzine B to ethanol is 0.1 mg: 1 mL.
[0032] Example 3 A plant growth regulator for prolonging the flowering period of garden flowers, comprising the following raw materials: Theaflavins, huperzine B, Elaeagnus angustifolia leaf oil, salicylic acid, calcium chloride, 6-benzylaminopurine; The concentrations of theaflavins were 30 mg / L, huperzine B was 0.4 mg / L, Elaeagnus pungens leaf oil was 300 mg / L, salicylic acid was 10 mg / L, calcium chloride was 120 mg / L, and 6-benzylaminopurine was 7 mg / L.
[0033] The preparation method of the Elaeagnus pungens leaf oil is as follows: Take dried Elaeagnus pungens leaves, grind them into powder, add a complex hydrolytic enzyme solution of cellulase, hemicellulase and β-glucosidase, and enzymatically hydrolyze for 6 hours at 30-40℃. Then add ethanol and mix, and bathe in a water bath at 50℃ for 2 hours. Filter, and concentrate the filtrate by rotary evaporation to an oily state. Wash the oil phase with a small amount of distilled water to remove water-soluble impurities, and obtain Elaeagnus pungens leaf oil.
[0034] The mass-to-volume ratio of Elaeagnus pungens leaves, compound hydrolytic enzyme solution, and ethanol is 200g:1L:1L.
[0035] The complex hydrolase contains 15 g / L cellulase, 8 g / L hemicellulase, and 22 g / L β-glucosidase.
[0036] A plant growth regulator for prolonging the flowering period of garden flowers is prepared by the following steps: (1) Weigh out the required concentration of 6-benzylaminopurine and dissolve it in dimethyl sulfoxide to obtain a 6-benzylaminopurine solution. Then, weigh out theaflavin and huperzine B according to the required concentration and dissolve them in ethanol to obtain theaflavin solution and huperzine B solution respectively. (2) Mix Elaeagnus pungens oil and Tween-80 at a volume ratio of 1:2 to obtain an emulsion; (3) Take a dilution container, weigh calcium chloride and salicylic acid according to the required concentration, add water and stir until dissolved, then add the 6-benzylaminopurine solution, theaflavin solution and huperzine B solution from step (1) respectively, continue to add the emulsion from step (2), and finally add water to make up the volume and stir evenly to obtain the final product plant growth regulator.
[0037] In step (1), the amount of dimethyl sulfoxide used is just enough to dissolve 6-benzylaminopurine; the ratio of theaflavins to ethanol is 10 mg: 1 mL; and the ratio of huperzine B to ethanol is 0.1 mg: 1 mL.
[0038] Comparative Example 1 A plant growth regulator for prolonging the flowering period of garden flowers, comprising the following raw materials: Huperzine B, Elaeagnus pungens leaf oil, salicylic acid, calcium chloride, 6-benzylaminopurine; The concentrations of huperzine B were 0.4 mg / L, Elaeagnus pungens leaf oil was 300 mg / L, salicylic acid was 10 mg / L, calcium chloride was 120 mg / L, and 6-benzylaminopurine was 7 mg / L.
[0039] The preparation method of the Elaeagnus pungens leaf oil is as follows: Take dried Elaeagnus pungens leaves, grind them into powder, add a complex hydrolytic enzyme solution of cellulase, hemicellulase and β-glucosidase, and enzymatically hydrolyze for 6 hours at 30-40℃. Then add ethanol and mix, and bathe in a water bath at 50℃ for 2 hours. Filter, and concentrate the filtrate by rotary evaporation to an oily state. Wash the oil phase with a small amount of distilled water to remove water-soluble impurities, and obtain Elaeagnus pungens leaf oil.
[0040] The mass-to-volume ratio of Elaeagnus pungens leaves, compound hydrolytic enzyme solution, and ethanol is 200g:1L:1L.
[0041] The complex hydrolase contains 15 g / L cellulase, 8 g / L hemicellulase, and 22 g / L β-glucosidase.
[0042] A plant growth regulator for prolonging the flowering period of garden flowers is prepared by the following steps: (1) Weigh out the required concentration of 6-benzylaminopurine and dissolve it in dimethyl sulfoxide to obtain a 6-benzylaminopurine solution. Then, weigh out the required concentration of huperzine B and dissolve it in ethanol to obtain a huperzine B solution. (2) Mix Elaeagnus pungens oil and Tween-80 at a volume ratio of 1:2 to obtain an emulsion; (3) Take a dilution container, weigh calcium chloride and salicylic acid according to the required concentration, add water and stir until dissolved, then add the 6-benzylaminopurine solution and huperzine B solution from step (1) respectively, continue to add the emulsion from step (2), and finally add water to make up the volume and stir evenly to obtain the final product plant growth regulator.
[0043] In step (1), the amount of dimethyl sulfoxide used is just enough to dissolve 6-benzylaminopurine; the ratio of huperzine B to ethanol is 0.1 mg: 1 mL.
[0044] Compared with Example 3, this comparative example is identical to Example 3 except that theaflavins are not added. All other raw materials and steps are the same as in Example 3.
[0045] Comparative Example 2 A plant growth regulator for prolonging the flowering period of garden flowers, comprising the following raw materials: Theaflavins, Elaeagnus angustifolia leaf oil, salicylic acid, calcium chloride, 6-benzylaminopurine; The concentrations of theaflavins were 30 mg / L, the Elaeagnus pungens leaf oil was 300 mg / L, the salicylic acid was 10 mg / L, the calcium chloride was 120 mg / L, and the 6-benzylaminopurine was 7 mg / L.
[0046] The preparation method of the Elaeagnus pungens leaf oil is as follows: Take dried Elaeagnus pungens leaves, grind them into powder, add a complex hydrolytic enzyme solution of cellulase, hemicellulase and β-glucosidase, and enzymatically hydrolyze for 6 hours at 30-40℃. Then add ethanol and mix, and bathe in a water bath at 50℃ for 2 hours. Filter, and concentrate the filtrate by rotary evaporation to an oily state. Wash the oil phase with a small amount of distilled water to remove water-soluble impurities, and obtain Elaeagnus pungens leaf oil.
[0047] The mass-to-volume ratio of Elaeagnus pungens leaves, compound hydrolytic enzyme solution, and ethanol is 200g:1L:1L.
[0048] The complex hydrolase contains 15 g / L cellulase, 8 g / L hemicellulase, and 22 g / L β-glucosidase.
[0049] A plant growth regulator for prolonging the flowering period of garden flowers is prepared by the following steps: (1) Weigh out the required concentration of 6-benzylaminopurine and dissolve it in dimethyl sulfoxide to obtain a 6-benzylaminopurine solution. Then weigh out theaflavin according to the required concentration and dissolve it in ethanol to obtain a theaflavin solution. (2) Mix Elaeagnus pungens oil and Tween-80 at a volume ratio of 1:2 to obtain an emulsion; (3) Take a dilution container, weigh calcium chloride and salicylic acid according to the required concentration, add water and stir until dissolved, then add the 6-benzylaminopurine solution and theaflavin solution from step (1) respectively, continue to add the emulsion from step (2), and finally add water to make up the volume and stir evenly to obtain the final product plant growth regulator.
[0050] In step (1), the amount of dimethyl sulfoxide used is just enough to dissolve 6-benzylaminopurine; the ratio of theaflavins to ethanol is 10 mg: 1 mL.
[0051] Compared with Example 3, this comparative example is identical to Example 3 except that huperzine B is not added. All other raw materials and steps are the same as in Example 3.
[0052] Comparative Example 3 A plant growth regulator for prolonging the flowering period of garden flowers, comprising the following raw materials: Theaflavins, huperzine B, salicylic acid, calcium chloride, 6-benzylaminopurine; The concentrations of theaflavins were 30 mg / L, huperzine B was 0.4 mg / L, salicylic acid was 10 mg / L, calcium chloride was 120 mg / L, and 6-benzylaminopurine was 7 mg / L.
[0053] A plant growth regulator for prolonging the flowering period of garden flowers is prepared by the following steps: (1) Weigh out the required concentration of 6-benzylaminopurine and dissolve it in dimethyl sulfoxide to obtain a 6-benzylaminopurine solution. Then, weigh out theaflavin and huperzine B according to the required concentration and dissolve them in ethanol to obtain theaflavin solution and huperzine B solution respectively. (2) Take a dilution container, weigh calcium chloride and salicylic acid according to the required concentration, add water and stir until dissolved, then add the 6-benzylaminopurine solution, theaflavin solution and huperzine B solution from step (1) respectively, and finally add water to make up the volume and stir evenly to obtain the final product plant growth regulator.
[0054] In step (1), the amount of dimethyl sulfoxide used is just enough to dissolve 6-benzylaminopurine; the ratio of theaflavins to ethanol is 10 mg: 1 mL; and the ratio of huperzine B to ethanol is 0.1 mg: 1 mL.
[0055] Compared with Example 3, this comparative example is identical to Example 3 except that Elaeagnus pungens leaf oil is not added. All other raw materials and steps are the same as in Example 3.
[0056] Performance testing Experiment on the effect of the plant growth regulator of this invention on the flowering period of Lagerstroemia indica The crape myrtle variety used in this experiment was "Purple Elf." Two-year-old seedlings with uniform growth were selected as experimental materials and randomly divided into 7 groups of 20 seedlings each. The plant growth regulators obtained in Examples 1-3 and Comparative Examples 1-3 were sprayed starting in early June, with water spraying serving as a blank control. Spraying was conducted every two weeks for a total of 3 times, in the evening, spraying both leaves and flowers, counting each drop of water as one application. The growth and flowering period of the crape myrtle flowers were tracked and recorded. At the initial flowering stage, approximately 5% of the flower buds were open, with most remaining closed; at the beginning of peak flowering, approximately 25% of the flower buds were open; and at the beginning of late flowering, approximately 95% of the flower buds were open. The duration of peak flowering was from the beginning to the end of peak flowering for a single plant; the duration of flowering was from the beginning to the beginning of late flowering. At the beginning of peak flowering, 5 plants were randomly selected from each treatment to investigate and determine the number of flowers on each inflorescence. All data were recorded in Table 1.
[0057] Table 1. Effects of different treatments on crape myrtle flowers As shown in Table 1, compared with the blank control, the number of flowers on the inflorescence increased, the flowering period was advanced, and the number of days of flowering was significantly extended after spraying the plant growth regulator of the present invention. However, compared with Example 3, the number of flowers on the inflorescences in Comparative Examples 1-3, which changed the regulator components, decreased to varying degrees, and the number of days of flowering was also reduced to varying degrees. This demonstrates that the three components added to the plant growth regulator of the present invention—theaflavins, huperzine B, and Elaeagnus pungens leaf oil—are all indispensable.
[0058] Experiment on the effects of the plant growth regulator of this invention on roses The rose variety used in this experiment was the floribunda rose 'Golden Mary'. Three-year-old plants with uniform growth were selected as experimental materials and randomly divided into 7 groups of 10 plants each, with three replicates. Starting in mid-April, the plant growth regulators obtained in Examples 1-3 and Comparative Examples 1-3 were sprayed, with water spraying serving as a blank control. Spraying was conducted every two weeks for a total of 3 times, with spraying done in the evening, spraying both leaves and flowers, counting each drop of water as one application. The growth and flowering period of the roses were tracked and recorded. The initial flowering period was defined as the date when approximately 5% of the flowers opened; the early full bloom period as the date when approximately 25% of the flowers opened; and the beginning of the withering period as the date when approximately 50% of the petals began to fall. The duration of the flowering period was the time from the beginning of full bloom to the beginning of withering. During the full bloom period, 6 rose plants from each treatment group were selected, and the number of flowers was recorded. The data are recorded in Table 2.
[0059] Table 2. Effects of different treatments on roses As shown in Table 2, compared with the blank control, the initial flowering period and early full bloom of roses were earlier after using the plant growth regulator of this invention, indicating that the plant growth regulator of this invention can promote earlier flowering of roses. Furthermore, as shown in Table 2, compared with the blank control, the onset of petal withering was delayed after using the plant growth regulator of this invention, indicating that the plant growth regulator of this invention can delay petal withering and fall, significantly prolong the flowering period of roses, and enhance the viewing period of roses. Comparative Examples 1-3, which changed the regulator components, all showed varying degrees of reduction in flowering duration, indicating that theaflavins, huperzine B, and Elaeagnus pungens leaf oil in the plant growth regulator of this invention play a crucial role in prolonging the flowering period of roses.
[0060] Experiment on the effect of the plant growth regulator of this invention on the flowering period of Magnolia rubyana. The magnolia variety used in this experiment was "Jiaodan". The experiment was conducted at the magnolia planting base in Huanglou Town, Qingzhou City, Shandong Province. Six-year-old plants with uniform growth were selected as experimental materials and randomly divided into 7 groups of 10 plants each. Starting in mid-February, the plant growth regulators obtained in Examples 1-3 and Comparative Examples 1-3 were sprayed, with water spraying serving as a blank control. Spraying was conducted every two weeks for a total of 3 times, with spraying done in the evening. Large areas of flower buds or flowers were sprayed, with each drop of water counted as one application. The growth and flowering period of the magnolia flowers were tracked and recorded. The initial flowering period was defined as the date when 5% of the plants began to bloom, the peak flowering period as the date when 50% of the plants began to bloom, and the final flowering period as the date when 75% of the plants' flowers fell. The viewing period was the duration from the initial flowering period to the final flowering period. The data are recorded in Table 3.
[0061] Table 3. Effects of different treatments on magnolia blooming period As shown in Table 3, compared with the blank control, the initial flowering period and full bloom period of red magnolia were advanced after using the plant growth regulator of this invention, indicating that the plant growth regulator of this invention can promote earlier flowering of magnolia. In addition, the flowering period of magnolia was delayed after using the plant growth regulator of this invention. Combined with the above data, it is clear that using the plant growth regulator of this invention can significantly prolong the flowering period of magnolia and enhance its ornamental value.
[0062] It should be noted that the above embodiments are merely some preferred embodiments of the present invention, and not all embodiments. Obviously, based on the above embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort should fall within the scope of protection of the present invention.
Claims
1. A plant growth regulator for prolonging the flowering period of garden flowers, characterized in that, Including the following raw materials: The ingredients include theaflavins, huperzine B, Elaeagnus angustifolia leaf oil, salicylic acid, calcium chloride, and 6-benzylaminopurine; wherein the concentration of theaflavins is 10-30 mg / L, the concentration of huperzine B is 0.2-0.4 mg / L, the concentration of Elaeagnus angustifolia leaf oil is 100-300 mg / L, the concentration of salicylic acid is 5-10 mg / L, the concentration of calcium chloride is 80-120 mg / L, and the concentration of 6-benzylaminopurine is 4-7 mg / L. The preparation method of the Elaeagnus pungens leaf oil is as follows: Take dried Elaeagnus pungens leaves, grind them into powder, add a complex hydrolytic enzyme solution of cellulase, hemicellulase and β-glucosidase, and enzymatically hydrolyze at 30-40℃ for 6 hours. Then add ethanol and mix, and bathe in a water bath at 50℃ for 2 hours. Filter, and concentrate the filtrate by rotary evaporation to an oily state. Wash the oil phase with a small amount of distilled water to remove water-soluble impurities and obtain Elaeagnus pungens leaf oil. The preparation method of the plant growth regulator for prolonging the flowering period of garden flowers includes the following steps: (1) Weigh out the required concentration of 6-benzylaminopurine and dissolve it in dimethyl sulfoxide to obtain a 6-benzylaminopurine solution. Then, weigh out theaflavin and huperzine B according to the required concentration and dissolve them in ethanol to obtain theaflavin solution and huperzine B solution respectively. (2) Mix Elaeagnus pungens oil and Tween-80 at a volume ratio of 1:2 to obtain an emulsion; (3) Take a dilution container, weigh calcium chloride and salicylic acid according to the required concentration, add water and stir until dissolved, then add the 6-benzylaminopurine solution, theaflavin solution and huperzine B solution from step (1) respectively, continue to add the emulsion from step (2), and finally add water to make up the volume and stir evenly to obtain the final product plant growth regulator.
2. The plant growth regulator for prolonging the flowering period of garden flowers according to claim 1, characterized in that, The mass-to-volume ratio of Elaeagnus pungens leaves, compound hydrolytic enzyme solution, and ethanol is 200g:1L:1L.
3. The plant growth regulator for prolonging the flowering period of garden flowers according to claim 1, characterized in that, The complex hydrolase contains 12-15 g / L of cellulase, 5-8 g / L of hemicellulase, and 14-22 g / L of β-glucosidase.
4. The plant growth regulator for prolonging the flowering period of garden flowers according to claim 1, characterized in that, The plant growth regulator is a liquid.
5. The application of the plant growth regulator according to claim 1 in prolonging the flowering period of garden flowers, characterized in that, The garden flowers mentioned are roses, red magnolias, and crape myrtles.