一种多步酶解的黄芪甲苷制备工艺
By employing multi-step enzymatic hydrolysis and segmented ultrasonic permeation technology, combined with ultrafiltration and nanofiltration separation, the problem of astragaloside A's difficulty in dissolving was solved, achieving highly efficient extraction of astragaloside A with significantly improved recovery rate and purity.
Patent Information
- Application Number
- CN202511727457.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2025-11-24
- Publication Date
- 2026-07-17
- Estimated Expiration
- 2045-11-24
AI Technical Summary
The natural content of astragaloside A is extremely low, and it is wrapped in the dense structure of the astragalus cell wall, making it difficult for the active ingredient to dissolve. Existing extraction processes cannot achieve high recovery and high purity of astragaloside A.
A multi-step enzymatic hydrolysis process combined with segmented ultrasonic permeation and ultrafiltration-nanofiltration tandem technology was adopted. The cell wall structure was destroyed by the synergistic action of enzymes such as pectin esterase and rhamnogalacturonate lyase. Impurities were gradually separated by ethanol concentration gradient and anhydrous sodium sulfate salting out. Finally, astragaloside A was separated by 10 kDa ultrafiltration and 300-800 Da nanofiltration.
It significantly improved the recovery rate and purity of astragaloside A, achieving efficient extraction of astragaloside A with a recovery rate of 88.3-94.2% and a significant increase in the purity of the crude product.
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Abstract
Citation Information
Patent Citations
Method for preparing astragaloside from astragalus root
CN101759755A
Method for converting astragalosides to prepare cycloastragenol by two-step enzymolysis
CN107058445A