一种万寿菊一步成苗组织培养方法

By culturing marigold stem segments with buds in induction, proliferation, and seedling strengthening media and then directly inserting them into the cultivation substrate to simultaneously complete rooting, the problems of high cost, long cycle, and low survival rate in the rooting stage of existing technologies are solved. This achieves efficient one-step seedling production, improving production efficiency and seedling rate.

CN121844956BActive Publication Date: 2026-07-17FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI +1

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
Filing Date
2026-03-19
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing marigold tissue culture technology requires the separate preparation of special culture medium for the rooting stage, which increases costs and operational complexity. The separate rooting and transplanting processes result in long cycles, low survival rates, and susceptibility to microbial contamination, leading to low production efficiency.

Method used

After marigold stem segments with buds are cultured in induction, proliferation and seedling strengthening media, they are directly inserted into the cultivation substrate to simultaneously complete rooting and environmental adaptation, simplifying the seedling production method into a one-step process and eliminating the need for preparing and transferring rooting media.

Benefits of technology

It significantly reduces costs, shortens the tissue culture cycle, improves rooting rate and transplant survival rate, increases production efficiency, and achieves a seedling survival rate of over 90%, meeting the needs of large-scale production.

✦ Generated by Eureka AI based on patent content.

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Abstract

本发明提供了一种万寿菊一步成苗组织培养方法,属于植物组织培养技术领域。本发明以万寿菊带芽茎段为外植体,依次接种在诱导培养基、增殖培养基和壮苗培养基进行培养,壮苗结束后,直接将组培苗插入生根盒的栽培基质中生根培养。本发明摒弃了传统组织培养技术中单独的生根培养基培养步骤,将壮苗后的组培苗直接插入栽培基质中同步完成生根与环境适应,减少了组培苗转移操作环节,显著降低了育苗成本,缩短了育苗周期,并提高了成苗稳定性。
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