Tsetse fly universal detection primer pair and digital PCR detection method
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI
- Filing Date
- 2026-03-18
- Publication Date
- 2026-06-09
AI Technical Summary
Existing trypanosome detection methods, such as traditional PCR and real-time quantitative PCR, are insufficient in sensitivity and accuracy, making it difficult to effectively detect very early or low parasitemia. They are also susceptible to interference from inhibitors in the sample. Digital PCR lacks specially optimized primer pairs, leading to false negative or false positive results.
Two primer pairs specifically amplifying the conserved regions of the Trypanosoma 18S rRNA gene were designed, and the digital PCR reaction system, including annealing temperature and primer concentration, was optimized to establish a highly sensitive and absolutely quantitative detection method.
It achieves highly sensitive detection of Trypanosoma, accurately identifying it at extremely low copy numbers, and is suitable for the diagnosis of Trypanosoma infection with very low viral load or early infection, reducing the risk of false positives. It is applicable to the detection of whole blood and purified DNA samples.
Smart Images
Figure FT_1 
Figure FT_2 
Figure FT_3