用于白及不同生长时期不同组织实时荧光定量PCR检测的内参基因
By using BsGAPDH and BsEIF as internal reference genes in different growth stages and tissues of Bletilla striata, specific amplification primers were designed to solve the accuracy problem of quantitative PCR detection of Bletilla striata, achieving stability and accuracy in gene expression analysis and supporting the study of pharmacodynamic components metabolism.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- YUNNAN AGRICULTURAL UNIVERSITY
- Filing Date
- 2026-03-16
- Publication Date
- 2026-07-17
AI Technical Summary
The lack of stable internal reference genes applicable to different tissues and growth stages of Bletilla striata in existing technologies leads to insufficient accuracy and reliability of quantitative real-time PCR detection, affecting research on the metabolic patterns and quality formation mechanisms of pharmacodynamic components.
Using BsGAPDH and BsEIF as internal reference genes, specific amplification primers were designed for real-time quantitative PCR detection of Bletilla striata at different growth stages and/or in different tissues. Corresponding kits and detection methods are provided to ensure the stability and accuracy of gene expression analysis.
This improved the stability and accuracy of gene expression analysis in different tissues at different growth stages of Bletilla striata, providing reliable data support for the study of pharmacodynamic components metabolism and ensuring the reliability and consistency of the test results.
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Figure CN121852608B_ABST