一种以紫皮石斛茎段为起始材料的组培快繁的方法
The rapid propagation technology of Dendrobium officinale stem segments through tissue culture has solved the problems of low propagation coefficient and long cycle of traditional propagation methods, and achieved efficient and stable propagation of excellent traits, shortened the industrialization cycle, and met the market's rapid demand for high-quality seedlings.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- SOUTHWEST FORESTRY UNIVERSITY
- Filing Date
- 2026-03-19
- Publication Date
- 2026-07-17
AI Technical Summary
The seeds of Dendrobium nobile are tiny and difficult to germinate, making reproduction difficult under natural conditions. Traditional asexual reproduction methods have a low reproduction coefficient and a long cycle, which cannot meet the industry's rapid demand for high-quality seedlings.
Using stem segments of Dendrobium nobile as explants, rapid propagation was achieved through tissue culture techniques, including axillary bud explant preparation, explant disinfection, primary culture, proliferation culture, and seedling rooting culture. Hydrogen peroxide silver ion disinfectant solution was used to promote axillary bud germination, and potassium sorbate was used to rescue the culture medium in case of contamination, thus achieving efficient and stable rapid propagation.
The large-scale supply of superior single-plant seedlings was achieved in about two years, shortening the industrialization cycle of traditional methods by 75%, preserving the superior traits of the maternal parent, improving the survival rate and utilization rate of explants, and reducing costs.
Smart Images
Figure CN121867100B_ABST