A liposome / polymer / nucleic acid complex delivery system with both aqueous phase storage stability and high transfection efficiency
By using a method of preparing polymer/nucleic acid complexes encapsulated in liposomes, and combining cationic liposomes with hyperbranched polylysine, the problems of insufficient storage stability and transfectivity of lipid-based nucleic acid carriers in aqueous environments have been solved, achieving a balance between long-term stability and high transfection efficiency.
CN121868467BActive Publication Date: 2026-06-26ZHEJIANG UNIV
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- ZHEJIANG UNIV
- Filing Date
- 2026-03-23
- Publication Date
- 2026-06-26
Smart Images
Figure CN121868467B_ABST
Abstract
The application discloses a liposome / polymer / nucleic acid composite delivery system with water-phase storage stability and high transfection, and belongs to the technical field of biomaterials. The liposome / polymer / nucleic acid composite prepared by the method has a cationic lipid layer and a hyperbranched polylysine / nucleic acid composite particle core. The hyperbranched polylysine / mRNA particle has enhanced hydrolysis resistance, and the water-phase storage stability of the composite is enhanced, and the high transfection activity can be maintained for up to three months under the condition of 4 DEG C water phase. In addition, the hyperbranched polylysine involved in the application can promote efficient release of mRNA, so that the stability of the composite carrier is enhanced, and efficient transfection is realized. The method provided by the application is simple in operation, high in repeatability, and has a good application prospect in the field of mRNA vaccines.
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