蜂蜜中外源淀粉酶AB的定性检测方法及其应用
By using MALDI-TOF-MS technology and employing characteristic peaks to qualitatively detect exogenous amylase AB in honey, this method solves the problem that existing technologies cannot identify honey adulteration. It achieves highly specific and sensitive identification of exogenous amylase AB in honey, supporting the identification of genuine honey and quality evaluation.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- 秦皇岛海关技术中心
- Filing Date
- 2026-03-23
- Publication Date
- 2026-07-17
AI Technical Summary
Existing methods for detecting amylase activity cannot identify exogenous amylases added to honey, making it difficult to distinguish between adulterated and low-quality honey.
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was used to qualitatively detect exogenous amylase AB in honey by means of characteristic peaks. The process included sample processing, protein extraction, desalting, and spotting steps, and qualitative analysis was performed under specific detection conditions.
It achieves high specificity and sensitivity in the qualitative identification of exogenous amylase AB in honey, and can easily and effectively identify honey adulteration. It is characterized by simple operation and high efficiency, and supports the identification of the authenticity and quality evaluation of honey.
Smart Images
Figure CN121878008B_ABST