A firming and brightening composition containing white truffle extract and uses thereof

Abstract

This invention relates to the field of cosmetic technology, specifically to a firming and brightening composition containing white truffle extract and its application. The composition includes white truffle extract, Dendrobium nobile stem extract, Spirulina macrophylla extract, and Pyracantha fortuneana fruit extract. The composition obtained by combining the above raw materials can significantly inhibit the activity of elastase and collagenase, and has a certain whitening effect while being gentle and non-irritating.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, specifically to a firming and brightening composition containing white truffle extract and its application. Background Technology

[0002] Skin aging is a complex physiological process, mainly manifested as skin sagging, loss of elasticity, wrinkles, and dull, lackluster skin tone. This is not only an inevitable result of aging, but also closely related to external environmental stimuli (such as ultraviolet radiation, blue light radiation, and pollution) and internal factors (such as slowed cell metabolism and oxidative stress). In the field of cosmetic technology, developing products with both firming and brightening effects has always been a key focus and challenge in research and development.

[0003] Currently available anti-aging products often focus on single or limited targets. For example, in skin firming, most technologies emphasize supplementing or promoting collagen synthesis, such as the widespread use of vitamin C and its derivatives, retinol, and various small molecule peptides. These ingredients can improve skin elasticity to some extent by providing raw materials for collagen synthesis or stimulating fibroblast activity. However, skin firmness depends not only on collagen abundance but also on the integrity of the elastin network and the health of the extracellular matrix. Simply promoting collagen synthesis often fails to comprehensively prevent the degradation and breakage of elastic fibers, resulting in incomplete and short-lasting firming effects.

[0004] In terms of brightening skin tone, mainstream technologies mostly target the inhibition of melanin production, such as using tyrosinase inhibitors like arbutin, niacinamide, and kojic acid. While these ingredients can effectively reduce pigmentation, their effectiveness is limited in improving dullness and poor radiance caused by insufficient moisture in the stratum corneum, poor microcirculation, and the accumulation of aging cells. Dull skin tone is often the result of a combination of factors, and targeting only the melanin pathway is insufficient to achieve true "brightness" and translucency.

[0005] More importantly, skin laxity and dullness are not isolated phenomena, but rather intrinsically interconnected. Collagen loss and elastin degradation lead to the collapse of the skin's supporting structure, causing disordered light reflection on the skin surface and making it appear duller. Conversely, chronic oxidative stress and inflammatory responses not only promote pigmentation but also accelerate the degradation of the dermal matrix. Therefore, existing single-target or simple combination solutions are insufficient to address these complex issues at their root, often resulting in a partial solution that fails to achieve the synergistic effect of "firming" and "brightening."

[0006] In addition, in pursuit of immediate or potent effects, some products have to use high concentrations of active ingredients (such as glycolic acid and high concentrations of retinol), which may lead to risks such as skin irritation, dryness, and barrier damage. For people with sensitive skin or unstable skin conditions, their gentleness and safety are challenged.

[0007] In conclusion, there is an urgent need in this field for a novel technological solution that can effectively address the multiple causes of skin laxity and dullness through the synergistic effects of multiple targets and pathways. An ideal product should inhibit the activity of key aging enzymes (such as elastase and collagenase) while simultaneously improving skin tone, and ensure the gentleness and safety of the formula to meet consumers' growing demand for highly effective, comprehensive, and safe anti-aging skincare. Summary of the Invention

[0008] To achieve the above objectives and overcome the shortcomings of the prior art, the present invention provides a firming and brightening composition containing white truffle extract and its application.

[0009] In a first aspect, the present invention provides a firming and brightening composition containing white truffle extract, the composition comprising the following raw materials in parts by weight: White truffle extract: 0.1-3 parts; Dendrobium nobile stem extract: 0.2-1 part; Spirulina macrophylla extract: 0.5-3 parts; Pyracantha fruit extract: 0.1-0.8 parts.

[0010] in: White truffle extract can significantly enhance autophagy. Autophagy is the self-renewal process by which cells clear away aging and damaged components, and it is crucial for maintaining healthy and youthful skin. White truffle extract can increase the expression of ATG7 (an autophagy marker) protein, thereby promoting cell metabolism and renewal, helping to repair damaged skin, and making room for the synthesis of new collagen. At the same time, white truffle extract can also directly promote the synthesis of various collagens (including type I, type III, and even types IV and V), and is rich in antioxidants such as polyphenols, which can neutralize free radicals, slow down skin aging caused by oxidative damage, and improve skin elasticity, radiance, and firmness in many ways.

[0011] The core efficacy of Dendrobium nobile stem extract lies in its exceptional moisturizing and repairing abilities. Its rich polysaccharide content effectively locks in moisture, maintaining the hydration level of the stratum corneum, leaving skin plump and supported, resulting in a visually firmer appearance. Furthermore, its flavonoid components possess antioxidant properties, helping to eliminate free radicals and reduce oxidative stress damage. Additionally, Dendrobium nobile stem extract has a certain inhibitory effect on tyrosinase activity, helping to reduce excessive melanin production at its source, thus contributing to brightening and evening out skin tone.

[0012] Spirulina macrophylla extract is rich in nutrients such as vitamins, minerals, amino acids, and plant proteins. It can improve the overall metabolic vitality of skin cells, promote blood circulation, bring more oxygen and nutrients to skin cells, and accelerate the removal of waste, thereby enhancing the skin's radiance and healthy vitality. Its active ingredients can also regulate cell metabolism, promote the synthesis of collagen in the dermis, and help neutralize acidic metabolites in the skin, maintaining a healthy skin environment.

[0013] Pyracantha fruit extract contains high concentrations of natural vitamin C and flavonoids. Vitamin C is an essential cofactor for collagen synthesis, directly promoting collagen production and enhancing skin elasticity. Simultaneously, vitamin C effectively inhibits tyrosinase activity, blocking the melanin production pathway at its source, thus achieving whitening and fading dark spots. The synergistic effect of vitamin C and flavonoids powerfully scavenges free radicals, providing antioxidant protection and delaying skin oxidative aging.

[0014] The present invention has found that the combined use of the above-mentioned raw materials has a synergistic effect and can achieve excellent firming effect.

[0015] Preferably, the composition comprises the following parts by weight of raw materials: White truffle extract: 1.5 parts; Dendrobium nobile stem extract: 0.5 parts; Spirulina macrophylla extract: 2.6 parts; Pyracantha fruit extract: 0.4 parts.

[0016] Secondly, the present invention provides the use of the composition described in the first aspect in the preparation of cosmetics having firming and brightening effects.

[0017] Preferably, the dosage form of the cosmetic includes any one of the following: aqueous solution, lotion, face cream, serum, face mask, and eye cream.

[0018] Thirdly, the present invention provides a serum with firming and brightening effects, the serum comprising the firming and brightening composition containing white truffle extract as described in the first aspect.

[0019] Preferably, the essence further includes skin conditioning agents, thickeners, preservatives, and solvents.

[0020] More preferably, the skin conditioning agent includes at least one of glycerin, butylene glycol, sodium hyaluronate, and panthenol.

[0021] More preferably, the thickener includes at least one of sclerotinia gum, ammonium acryloyldimethyl taurate / VP copolymer, xanthan gum, and carbomer.

[0022] More preferably, the preservative is at least one of succinyl ketone, 1,2-hexanediol, and 1,2-pentanediol.

[0023] More preferably, the solvent is deionized water.

[0024] Compared with the prior art, the beneficial effects of the present invention are as follows: This invention provides a composition obtained by compounding white truffle extract, Dendrobium nobile stem extract, Spirulina macrophylla extract, and Pyracantha fortuneana fruit extract in a specific mass ratio. When the above four raw materials are scientifically compounded, they can achieve a firming and brightening effect of "1+1>2" through multi-target and multi-pathway synergistic effects. The possible principle is as follows: A multi-target anti-aging network: Skin laxity and dullness are the result of multiple factors, including internal collagen loss, oxidative damage, slowed cell metabolism, and insufficient hydration. Pyracantha fruit extract and white truffle extract serve as core promoters, providing powerful antioxidant effects and directly promoting collagen regeneration. Dendrobium nobile stem extract provides an optimal environment for collagen synthesis through long-lasting hydration and strengthens the skin barrier. Spirulina macrophylla extract activates cell metabolism holistically and improves microcirculation. These ingredients work together on different levels to combat aging, creating a synergistic effect.

[0025] Repair and Promote Circulation: The enhanced autophagy capacity of white truffle extract is equivalent to a "deep clean" for skin cells, removing aging and damaged cells. This creates space and provides a high-quality "construction environment" for the synthesis of new collagen promoted by both firethorn fruit extract and white truffle extract. This "break-and-build" cycle is key to achieving deep firming and regeneration.

[0026] Hydration for Firmness and Radiance: The powerful moisturizing ability of Dendrobium nobile stem extract is crucial. When skin is well-hydrated, stratum corneum cells are plump, the epidermis is smooth, and it immediately appears firmer. At the same time, sufficient moisture is the foundation for all biochemical reactions, including collagen synthesis, to proceed efficiently. A hydrated skin base also reflects light better, appearing naturally radiant.

[0027] Internal revitalization and external protection: Spirulina macrophylla extract enhances overall skin vitality and radiance from within by promoting blood circulation and metabolism, while Pyracantha fortuneana and white truffle provide powerful external antioxidant protection, directly combating external factors that cause dullness. This holistic approach works together to achieve firmer and brighter skin.

[0028] In summary, the composition obtained by scientifically combining the above-mentioned raw materials has significant firming and brightening effects, and is gentle and non-irritating. The raw materials achieve a significant synergistic effect. Detailed Implementation

[0029] To better understand the present invention, the present invention will be further described below with reference to specific embodiments. The terms "composition" and "essence" used are for describing specific implementation schemes and do not constitute a limitation on the scope of protection of the present invention.

[0030] Unless otherwise specified, experimental methods in the following examples are generally performed under standard conditions or as recommended by the manufacturer. Unless otherwise stated, all measurements are by percentage and parts by mass.

[0031] Some of the raw materials and their sources are as follows: White truffle extract: purchased from PHENBIOX SRL; Dendrobium nobile stem extract: purchased from Yunnan Yingge Biotechnology Co., Ltd.; Spirulina macrophylla extract: purchased from Qingdao Zhongke Lanzhi Biotechnology Development Co., Ltd.; Pyracantha fruit extract: purchased from BASF (China) Co., Ltd.; All other raw materials and reagents are commercially available.

[0032] The raw materials, their mass fractions, and preparation method of the firming and brightening composition containing white truffle extract are as follows: Composition 1 Composed of the following raw materials by weight: White truffle extract: 1.5 parts; Dendrobium nobile stem extract: 0.5 parts; Spirulina macrophylla extract: 2.6 parts; Pyracantha fruit extract: 0.4 parts.

[0033] Preparation method: Mix the white truffle extract, Dendrobium nobile stem extract, Spirulina macrophylla extract, and Pyracantha fortuneana fruit extract evenly, seal, and store away from light.

[0034] Composition 2 Composed of the following raw materials by weight: White truffle extract: 0.1 part; Dendrobium nobile stem extract: 0.2 parts; Spirulina macrophylla extract: 0.5 parts; Pyracantha fruit extract: 0.1 part; The preparation method is the same as that of composition 1.

[0035] Composition 3 Composed of the following raw materials by weight: White truffle extract: 3 parts; Dendrobium nobile stem extract: 1 part; Spirulina macrophylla extract: 3 parts; Pyracantha fruit extract: 0.8 parts; The preparation method is the same as that of composition 1.

[0036] Composition ① Unlike composition 1, it lacks white truffle extract. The missing mass is made up by Dendrobium nobile stem extract, Spirulina macrophylla extract and Pyracantha fortuneana fruit extract in a mass ratio of 0.5:2.6:0.4. The other raw materials and mass are the same as those in composition 1. Preparation method: Mix Dendrobium nobile stem extract, Spirulina macrophylla extract and Pyracantha fortuneana fruit extract evenly, seal and store in the dark.

[0037] Composition ② Unlike composition 1, this composition lacks Dendrobium nobile stem extract. The missing mass fraction is made up by extracts of white truffle, Spirulina macrophylla, and Pyracantha fortuneana in a mass ratio of 1.5:2.6:0.4. The remaining raw materials and mass fractions are the same as those in composition 1. Preparation method: Mix the white truffle extract, Spirulina macrophylla extract and Pyracantha fruit extract evenly, seal and store in the dark.

[0038] Composition ③ Unlike composition 1, it lacks Spirulina macrophylla extract. The missing mass is made up by extracts of white truffle, Dendrobium nobile stem extract, and Pyracantha fortuneana fruit extract in a mass ratio of 1.5:0.5:0.4. The other raw materials and mass are the same as those in composition 1. Preparation method: Mix the white truffle extract, Dendrobium nobile stem extract, and Pyracantha fortuneana fruit extract evenly, seal, and store in the dark.

[0039] Composition ④ Unlike composition 1, this composition lacks Pyracantha fruit extract. The missing mass fraction is made up by extracts of white truffle, Dendrobium nobile stem extract, and Spirulina macrophylla extract in a mass ratio of 1.5:0.5:2.6. The remaining raw materials and mass fractions are the same as those in composition 1. Preparation method: Mix the white truffle extract, Dendrobium nobile stem extract, and Spirulina macrophylla extract evenly, seal, and store in the dark.

[0040] Composition ⑤ Composed of the following raw materials by weight: White truffle extract: 0.5 parts; Dendrobium nobile stem extract: 1.5 parts; Spirulina macrophylla extract: 0.4 parts; Pyracantha fruit extract: 2.6 parts; The preparation method is the same as that of composition 1.

[0041] The ingredients and their percentage by weight for this firming and brightening serum are as follows: Serum 1 Composed of the following components by mass percentage: Composition 1: 5wt% Skin conditioning agent: 3wt%; Preservative: 0.5 wt%; Thickener: 0.2 wt%; Solvent replenished to 100 wt%; The skin conditioning agent is glycerin; the preservative is acetone; the thickener is ammonium acryloyl dimethyl taurate / VP copolymer; and the solvent is deionized water. The preparation method of serum 1 includes the following steps: A1: Mix the skin conditioning agent, the composition, and 1 / 2 of the solvent evenly to obtain mixture A; A2: Mix the thickener with 1 / 4 of the solvent until homogeneous to obtain mixture B; A3: Mix mixture A, mixture B, preservative, and remaining solvent evenly to obtain the essence.

[0042] Serum 2 Composed of the following components by mass percentage: Composition 1: 1 wt% Skin conditioning agent: 3wt%; Preservative: 0.5 wt%; Thickener: 0.2 wt%; Deionized water to 100 wt%; The skin conditioning agent is glycerin; the preservative is acetone; the thickener is ammonium acryloyl dimethyl taurate / VP copolymer; and the solvent is deionized water. The preparation method is the same as that of essence 1.

[0043] Serum 3 Composed of the following components by mass percentage: Composition 1: 10 wt% Skin conditioning agent: 3wt%; Preservative: 0.5 wt%; Thickener: 0.2 wt%; Deionized water to 100 wt%; The skin conditioning agent is glycerin; the preservative is acetone; the thickener is ammonium acryloyl dimethyl taurate / VP copolymer; and the solvent is deionized water. The preparation method is the same as that of essence 1.

[0044] Serum 4 Composed of the following components by mass percentage: Composition 2: 5wt% Skin conditioning agent: 3wt%; Preservative: 0.5 wt%; Thickener: 0.2 wt%; Deionized water to 100 wt%; The skin conditioning agent is glycerin; the preservative is acetone; the thickener is ammonium acryloyl dimethyl taurate / VP copolymer; and the solvent is deionized water. The preparation method is the same as that of essence 1.

[0045] Serum 5 Composed of the following components by mass percentage: Composition 3: 5wt% Skin conditioning agent: 3wt%; Preservative: 0.5 wt%; Thickener: 0.2 wt%; Deionized water to 100 wt%; The skin conditioning agent is glycerin; the preservative is acetone; the thickener is ammonium acryloyl dimethyl taurate / VP copolymer; and the solvent is deionized water. The preparation method is the same as that of essence 1.

[0046] Serum ① Unlike serum 1, composition ① is used to replace composition 1 by the same mass percentage, while the remaining components and their mass percentages, as well as the preparation method and process parameters of the serum, are the same as those of serum 1.

[0047] Serum ② Unlike serum 1, composition 2 is used to replace composition 1 by the same mass percentage, while the remaining components and their mass percentages, as well as the preparation method and process parameters of the serum, are the same as those of serum 1.

[0048] Serum ③ Unlike serum 1, composition 3 is used to replace composition 1 by the same mass percentage. The remaining components and their mass percentages, as well as the preparation method and process parameters of the serum, are the same as those of serum 1.

[0049] Essence ④ Unlike serum 1, composition 4 is used to replace composition 1 by the same mass percentage. The remaining components and their mass percentages, as well as the preparation method and process parameters of the serum, are the same as those of serum 1.

[0050] Essence ⑤ Unlike serum 1, composition 5 is used to replace composition 1 by the same mass percentage. The remaining components and their mass percentages, as well as the preparation method and process parameters of the serum, are the same as those of serum 1.

[0051] Blank Essence Composed of the following components by mass percentage: Skin conditioning agent: 3wt%; Preservative: 0.5 wt%; Thickener: 0.2 wt%; Deionized water to 100 wt%; The skin conditioning agent is glycerin; the preservative is acetone; and the thickener is ammonium acryloyl dimethyl taurate / VP copolymer. The preparation method of blank serum includes the following steps: A1: Mix the skin conditioning agent with 1 / 2 of the deionized water until homogeneous to obtain mixture A; A2: Mix the thickener with 1 / 4 of the deionized water until homogeneous to obtain mixture B; A3: Mix mixture A, mixture B, preservative, and remaining deionized water evenly to obtain the essence.

[0052] Performance testing: elastase inhibition rate test This experiment used porcine pancreatic elastase as the test subject and N-succinyl-alanine-alanine-p-nitroaniline (AAAPVN) as the substrate. Porcine pancreatic elastase can hydrolyze AAAPVN, and its hydrolysis products can cause an increase in absorbance at a wavelength of 420 nm. The absorbance was measured using an enzyme-linked immunosorbent assay (ELISA) reader to evaluate the firming and anti-wrinkle effects of the test product.

[0053] Test sample: The composition was prepared into a 1wt% concentration test sample using deionized water, wherein the composition was any one of compositions 1-3 and compositions ①-⑤.

[0054] Solvent preparation: Prepare Tris-HCl buffer (0.1M pH=8.0): Weigh 2.42g Tris into a beaker, add 200mL of ultrapure water, dissolve completely, and then adjust the pH to 8.0 with concentrated HCl.

[0055] Preparation of positive control tea polyphenol solution (1 mg / ml): Weigh 5 mg of tea polyphenols and dissolve them in 5 ml of Tris-HCl buffer.

[0056] Prepare substrate solution AAAPVN (2mM): Weigh 4.51 mg N-succinyl-alanine-alanine-alanine-p-nitroaniline and dissolve it in 5 ml Tris-HCl buffer.

[0057] To prepare porcine pancreatic elastase solution (0.171 U / mL): Dissolve 280 μl in 10 mL of Tris-HCl buffer.

[0058] Grouping and sample addition: The experiment was divided into 4 groups: the test sample group, the positive control group, the blank control group, and the model control group. Each group had 4 replicates at the same concentration. The amount of each solution added is shown in Table 1.

[0059] Table 1 Experimental Groups Model control group 0 25 50 25 Blank control group 0 0 50 50 Positive control group (tea polyphenols 1 mg / mL) 25 25 50 0 Sample group to be tested 25 25 50 0 Test method: The reaction was allowed to proceed at room temperature for 15 minutes, and the absorbance was measured at 420 nm using an enzyme-linked immunosorbent assay (ELISA) reader.

[0060] Method for calculating elastase inhibition rate: Inhibition rate (%) = [1 - (A1 - A0) / (A2 - A0)] × 100% Where: A0 - the average absorbance of the blank control wells; A1 - The average absorbance of the sample well or the positive control well; The average absorbance of the A2 model control well.

[0061] Collagenase inhibition rate detection Collagenase can break down collagen. Collagen determines the properties of connective tissue and maintains the skin's resilience and firmness. High collagen content in the skin results in plump and firm skin, while skin lacking collagen is loose and inelastic. Therefore, detecting the inhibitory effect of test samples on collagenase can evaluate the skin-firming efficacy of the test samples.

[0062] Test sample: The composition was prepared into a 1wt% concentration test sample using deionized water, wherein the composition was any one of compositions 1-3 and compositions ①-⑤.

[0063] Reagent preparation: Tricine buffer: 50 mmol / L, pH 7.5.

[0064] Collagenase solution: Add 0.005 g of collagenase to Tricine buffer to make the final concentration of collagenase 0.8 U / mL.

[0065] FALGPA solution: Add 0.0096 g FALGPA to Tricine buffer to make the final concentration of FALGPA 2 mmol / L.

[0066] Test methods Group A: Mix 40 μL of test sample solution with 100 μL of Tricine buffer, then add 20 μL of 0.8 U / mL collagenase solution and mix well. Incubate at 25 °C for 15 min, then add 40 μL of 2 mmol / L FALGPA solution and mix well. Incubate at 25 °C for another 15 min, and then measure the absorbance value A of the solution at 335 nm.

[0067] Group B: Mix 40 μL of the test sample solution with 100 μL of Tricine buffer, then add 20 μL of Tricine buffer and mix well. Incubate at 25 °C for 15 min, then add 40 μL of Tricine buffer and mix well. Incubate at 25 °C for another 15 min. Measure the absorbance value B of the solution at 335 nm.

[0068] Group C: Mix 140 μL of Tricine buffer with 20 μL of 0.8 U / mL collagenase solution, incubate at 25℃ for 15 min, add 40 μL of 2 mmol / L FALGPA solution, mix well, and incubate at 25℃ for another 15 min. Measure the absorbance C of the solution at 335 nm.

[0069] Group D: Take 160 μL of Tricine buffer and incubate at 25 °C for 15 min. Then add 40 μL of Tricine buffer and mix well. Incubate at 25 °C for another 15 min. Measure the absorbance D of the solution at 335 nm.

[0070] The experiment was repeated three times, and the average value was taken. A 0.8 mg / mL solution of gallic acid catechin gallate (EGCG) was used as a positive control.

[0071] Method for calculating collagenase inhibition rate: Inhibition rate (%) = [(CD) - (AB)] / (CD) × 100% Where A, B, C, and D are absorbance values ​​A, B, C, and D, respectively.

[0072] The results of the elastase inhibition rate and collagenase inhibition rate tests are shown in Table 2 below.

[0073] Table 2. Results of the firming effect test of the composition Positive control group 73.4 61.7 Composition 1 70.5 58.5 Composition 2 68.9 56.7 Composition 3 69.3 56.1 Composition ① 34.2 37.2 Composition ② 53.1 43.7 Composition ③ 56.7 41.5 Composition ④ 49.3 38.4 Composition ⑤ 60.5 47.6 As shown in Table 2, compositions 1-3 and ①-⑤ can effectively inhibit the activity of elastase and collagenase, indicating that the compositions provided by the present invention have significant firming effects. Comparing the results of composition 1 with those of compositions ①-④, it can be seen that the extracts of white truffle, Dendrobium nobile stem, Spirulina macrophylla, and Pyracantha fortuneana fruit in the compositions have a certain synergistic effect, and their combined use can significantly enhance the firming effect of the compositions. Comparing the results of compositions 1-3 with those of composition ⑤, it can be seen that the ratio of the raw materials in the compositions affects the firming effect of the compositions. When the ratio of the raw materials falls within the range defined by the present invention, the resulting compositions have better firming effects.

[0074] Security test This invention experimentally verifies the biocompatibility of serums 1-5, serums ①-⑤, and a blank serum.

[0075] The irritation of cosmetics was evaluated using the 2015 Cosmetic Safety Technical Specifications as a reference standard. The test method was a skin patch test. The test was conducted on subjects aged 18-60 years who were randomly distributed. The subjects were randomly assigned to groups, with each subject corresponding to all test products, for a total of 30 subjects.

[0076] Test substances: serums 1-5, serums ①-⑤, and blank serum.

[0077] Test Method: Eleven test areas were divided on the subject's back. The test substance was placed in a patch applicator at a dosage of 0.020-0.025 g. The patch applicator containing the test substance was then covered with non-irritating adhesive tape over the test area on the subject's back. Gently press with the palm of the hand to ensure even adhesion to the skin surface. Each test area corresponded to one test substance. The test was conducted for 24 hours. Thirty minutes after removing the patch applicator, and once the pressure marks disappeared, the skin reaction was observed. If the result was negative, repeat observations were performed at 24 and 48 hours post-pattern testing.

[0078] Evaluation criteria: Grade 0: Negative reaction; Grade 1: Suspicious reaction, with only slight erythema; Grade 2: Weak positive reaction, erythema, infiltration, edema, and possible papules; Grade 3: Strong positive reaction, erythema, infiltration, edema, papules may be present, and the reaction may extend beyond the test area; Grade 4: Extremely positive reaction, with obvious erythema, severe infiltration, edema, confluent herpes, and reaction extending beyond the test area.

[0079] Test results: All subjects had negative skin reactions, indicating that the serum provided by this invention is safe and non-irritating.

[0080] Human efficacy test Test samples: serums 1-5, serums ①-⑤, blank serum.

[0081] Skin elasticity and firmness test: First, dermatologists screened volunteers, requiring them to have non-sensitive skin, not be in a sensitive state, have no skin diseases, and have no bacterial, fungal, or viral infections on their facial skin. They also had not used any anti-aging cosmetics, medications, health products, or cosmetic treatments within the three months prior to the trial, and any volunteers with other facial abnormalities that might affect the test results were excluded. Finally, the selected volunteers (aged 30-55) were divided into 11 groups of 20 each, and pre-treatment data were measured using the Multi Skin TestCenter, manufactured by Courag+Khazaka (CK GmbH, Germany). The MPA580 cutometer elastic probe measures the skin elasticity and firmness around the eyes (skin elasticity is judged by parameter R2, which is the ratio of the rebound amount Ua under no negative pressure to the maximum stretch Uf under negative pressure (i.e., R2=Ua / Uf), the higher the value, the better the skin elasticity; skin firmness is judged by parameter F4, obtained after 10 cycles of measurement with the cutometer probe, the lower the value, the firmer the skin); then a 28-day topical test begins, with each group corresponding to one test sample. During the 28-day test period, volunteers are given (2.0±0.1) mg / cm³ daily. 2 The test sample was applied to the face (once in the morning and once in the evening after cleansing the face with water), and no other cosmetics or skin care products were applied during the test. On day 29, the volunteers sat quietly for more than 30 minutes in an environment with a temperature of 21±1℃ and a humidity of 50%±10%. The skin elasticity R2 and skin firmness F4 around the eyes were measured using a Cutometer elasticity probe. Volunteers were not allowed to use any cosmetics or skin care products (including those used for the test sample) on the day of the test.

[0082] Skin whitening test: First, dermatologists screened volunteers who had non-sensitive skin, were not in a sensitive state, had no skin diseases, and whose facial skin was free of bacterial, fungal, or viral infections. Their melanin content was between 300-400 mg / cm², and they had not used any whitening cosmetics, medications, health products, or cosmetic treatments within the three months prior to the trial. Volunteers with any facial abnormalities that might affect the test results were excluded. The selected volunteers (aged 30-55) were then divided into 11 groups of 20 each. Pre-treatment data were measured using the MX18 melanin analyzer (manufactured by Courag+Khazaka, Germany, CK), with the testing point on the central forehead. (Higher melanin content indicates darker skin, and lower melanin content indicates lighter skin.) A 28-day topical treatment trial then began, with each group receiving one test sample. During the 28-day trial period, volunteers received (2.0±0.1) mg / cm² daily. 2 The test sample was applied to the face (once in the morning and once in the evening after cleansing the face with water), and no other cosmetics or skin care products were applied during the test. On day 29, the volunteers sat quietly for more than 30 minutes in an environment with a temperature of 21±1℃ and humidity of 50%±10%, and the melanin content after use was measured using an MX18 melanin analyzer. The test point was the middle of the forehead. On the day of the test, the volunteers could not use any cosmetics or skin care products (including those used for the test sample).

[0083] The results of the skin elasticity, firmness, and whitening tests are shown in Table 3.

[0084] The skin elasticity R2 change rate and skin firmness F4 change rate are calculated based on the formula: "Change rate (%) = (after use - before use) / before use × 100%".

[0085] The change rate of melanin content is calculated according to the formula: "Change rate (%) = (before use - after use) / before use × 100%".

[0086] Table 3 Results of Human Efficacy Tests Serum 1 20 9.6 -22.7 49.4 Serum 2 20 6.7 -13.4 22.6 Serum 3 20 10.2 -23.9 51.7 Serum 4 20 9.1 -21.3 43.5 Serum 5 20 9.3 -21.8 40.4 Serum ① 20 5.1 -10.9 36.4 Serum ② 20 7.1 -16.8 29.3 Serum ③ 20 7.6 -15.1 34.2 Essence ④ 20 6.3 -12.7 24.6 Essence ⑤ 20 8.6 -18.4 39.3 Blank Essence 20 0.9 -0.1 0.7 As shown in Table 3, comparing the results of serums 1-5, serums ①-⑤, and the blank serum, it is evident that the addition and amount of the composition significantly affect the firming effect of the serums. Comparing serums 1-3 and the blank serum, it is clear that the amount of the composition significantly affects the firming effect. Comparing serums 1-3, serum 1 offers the best cost-performance ratio. Comparing serum 1 with serums 4, 5, and ⑤, it is evident that the proportions of each ingredient in the composition of this invention significantly affect the firming effect of the serum. When the amount of ingredients used in the composition is outside the range defined by this invention, its efficacy is poor. Comparing serum 1 with serums ①-④, it is evident that there is a significant synergistic effect among the ingredients in the composition of this invention; when used together, they have a significant firming effect.

[0087] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments. Any changes, modifications, substitutions, combinations, or simplifications made without departing from the spirit and principle of the present invention shall be considered equivalent substitutions and shall be included within the protection scope of the present invention.

Claims

1. A firming and brightening composition comprising white truffle extract, characterized in that, The composition comprises the following raw materials in parts by weight: White truffle extract: 0.1-3 parts; Dendrobium nobile stem extract: 0.2-1 part; Spirulina macrophylla extract: 0.5-3 parts; Pyracantha fruit extract: 0.1-0.8 parts.

2. The composition of claim 1, wherein, The composition comprises the following raw materials in parts by weight: White truffle extract: 1.5 parts; Dendrobium nobile stem extract: 0.5 parts; Spirulina macrophylla extract: 2.6 parts; Pyracantha fruit extract: 0.4 parts.

3. The use of the composition as described in claim 1 or 2 in the preparation of a cosmetic having firming and brightening effects.

4. The use according to claim 3, wherein the compound is ###0002### The dosage form of the cosmetic includes any one of lotion, face cream, serum, face mask, and eye cream.

5. An essence having a firming and brightening effect, characterized in that, The serum comprises the composition of claim 1 or 2.

6. The essence as described in claim 5, characterized in that, The serum also includes skin conditioning agents, thickeners, preservatives, and solvents.

7. The essence as described in claim 6, characterized in that, The skin conditioning agent includes at least one of glycerin, butylene glycol, sodium hyaluronate, and panthenol.

8. The essence as described in claim 6, characterized in that, The thickener includes at least one of sclerotinia gum, ammonium acryloyldimethyl taurate / VP copolymer, xanthan gum, and carbomer.

9. The essence as described in claim 6, characterized in that, The preservative is at least one of succinyl ketone, 1,2-hexanediol, and 1,2-pentanediol.

10. The essence as described in claim 6, characterized in that, The solvent is deionized water.