一种响应胞外L-色氨酸浓度的纳升反应器及其制备方法与应用
By encapsulating L-tryptophan-producing bacteria and sensor bacteria in sodium alginate gel microspheres, fluorescence reporting of extracellular L-tryptophan concentration was achieved, solving the high-throughput and real-time problems of existing detection technologies and improving the efficiency of strain screening.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- WESTLAKE UNIV
- Filing Date
- 2026-03-26
- Publication Date
- 2026-07-17
AI Technical Summary
Existing L-tryptophan detection technologies cannot simultaneously meet the requirements of high throughput, microscale, and real-time detection. Extracellular detection methods are cumbersome and costly, while intracellular detection is susceptible to cross-leakage, affecting the assessment of strain productivity.
A nano-scale reactor was designed to encapsulate L-tryptophan-producing bacteria and sensor bacteria using sodium alginate gel microspheres. The sensor bacteria convert changes in extracellular L-tryptophan concentration into fluorescent reporters, which are then used for high-throughput screening via fluorescence imaging or flow cytometry.
It enables high-throughput detection and screening of L-tryptophan-producing strains, improves detection efficiency, overcomes the high cost and low throughput problems of existing technologies, and has versatility and scalability.
Smart Images
Figure CN121896260B_ABST