一种水稻叶色调控基因ALS8及其编码蛋白与应用

By knocking out the ALS8 gene using the CRISPR/Cas9 system, rice leaf color mutants were bred, solving the problem of unclear regulatory mechanisms of carbamoyltransferase genes. This enabled precise regulation of rice chloroplast development, improving rice breeding efficiency and seed purity.

CN121896280BActive Publication Date: 2026-07-17SANYA NATIONAL INSTITUTE OF SOUTHERN BREEDING CHINESE ACADEMY OF AGRICULTURAL SCIENCES +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SANYA NATIONAL INSTITUTE OF SOUTHERN BREEDING CHINESE ACADEMY OF AGRICULTURAL SCIENCES
Filing Date
2026-03-25
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

The regulatory mechanism of carbamoyltransferase genes in rice chloroplast development is unclear in the existing technology, and related gene resources are scarce, leading to chloroplast development disorders and reduced photosynthetic efficiency, which affect rice yield and quality.

Method used

By using the CRISPR/Cas9 gene editing system to knock out the ALS8 gene, rice leaf color mutants were prepared, enabling precise regulation of rice chloroplast development. This resulted in the cultivation of mutant plants with yellowing leaves, which can be used as seedling markers for hybrid rice seed production and molecular marker-assisted breeding.

Benefits of technology

It provides new genetic resources and experimental evidence, improves rice breeding efficiency, simplifies the hybrid rice seed production process, increases seed purity, and expands the application scenarios of rice breeding.

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Abstract

本发明属于基因工程领域,具体涉及一种水稻叶色调控基因ALS8及其编码蛋白与应用。所述ALS8基因的基因组gDNA序列如SEQ ID NO.1所示,编码区CDS序列如SEQ ID NO.2所示,其编码蛋白的氨基酸序列如SEQ ID NO.3所示。本发明通过CRISPR / Cas9技术构建ALS8基因敲除载体,获得ALS8功能缺失的水稻突变体,该突变体表现出叶绿体发育异常、叶绿素含量显著降低、苗期黄化致死的表型。实验证实ALS8基因在调控水稻叶绿体发育和叶色形成中具有关键作用。本发明提供的ALS8基因敲除突变体可作为叶色标记应用于杂交水稻制种过程中的苗期筛选,具有重要的育种应用价值和农业生产潜力。
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Citation Information

Patent Citations

  • Rice leaf whitening gene ALS as well as encoding protein and application thereof

    CN119932095A

  • Rice large-scale metabolism regulator DGP1 and crop metabolism quality design

    CN121160786A