百日咳杆菌检测用样本处理液及检测方法、产品和应用
By using a sample processing solution based on a eutectic solvent system, combined with specific components, the problem of poor sample processing in the detection of Bordetella pertussis has been solved, achieving efficient and accurate nucleic acid detection, which is suitable for primary healthcare institutions and on-site testing.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- SHANGHAI CHILDRENS MEDICAL CENT AFFILIATED TO SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
- Filing Date
- 2026-03-25
- Publication Date
- 2026-07-17
AI Technical Summary
Existing nucleic acid testing technologies suffer from poor sample pretreatment quality in the detection of Bordetella pertussis, resulting in low detection sensitivity, high false negatives, and complex and costly operation, making it difficult to meet the needs of primary healthcare institutions and on-site screening.
The sample processing solution, based on a eutectic solvent system, contains choline tartrate, betaine, glycerol, TCEP, IGEPA CA-630, HEPES, and EDTA. It is used to lyse Bordetella pertussis cells and remove matrix inhibitors. It is diluted with bovine serum albumin to simplify the operation process.
It significantly improves nucleic acid recovery rate, reduces inhibitor interference, enhances detection accuracy and sensitivity, adapts to sputum samples of different viscosities, simplifies operation, reduces costs, and is suitable for primary healthcare institutions and on-site testing.
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