A traditional Chinese medicine composition for preventing and treating salpingitis of chicken, a preparation method and application thereof

Abstract

The application provides a traditional Chinese medicine composition for preventing and treating oviduct inflammation of chickens, a preparation method and application thereof, and active ingredients of the traditional Chinese medicine composition include the following raw materials in parts by weight: Alisma orientalis (S. et Z.) Juz. 20-120, Coicis Semen 20-120, Alisma orientalis (L.) Juz. 10-40, Semen Persicae 10-30, Ligusticum Chuanxiong 10-40, Cyathula 15-50, Glycyrrhiza 5-20, and Fructus Alpiniae oxyphyllae 10-20. The traditional Chinese medicine composition can reduce edema and inflammation of the oviduct of the chicken, improve local blood circulation, promote tissue repair, and has excellent prevention and treatment effects on oviduct inflammation of the chicken.

Description

Technical Field

[0001] This invention belongs to the field of poultry medicine technology, specifically relating to a traditional Chinese medicine composition for the prevention and treatment of oviductitis in chickens, its preparation method, and its application. Background Technology

[0002] Oviductitis in laying hens is a common reproductive tract disease in intensive poultry farming, often leading to a decline in egg production performance, such as reduced egg production rate or even cessation of production; and abnormal egg quality, including soft-shelled eggs, deformed eggs, sandy-shelled eggs, freckled eggs, and extra-small eggs. These abnormalities are particularly pronounced in the later stages of egg production, and the risk of disease increases with age, causing significant economic losses to farms. Its pathogenic factors are complex, encompassing multiple aspects such as environmental hygiene, feed nutrition, pathogen infection, and husbandry management. Infection by pathogenic microorganisms from external sources is the core contributing factor. Clinical studies have isolated pathogens such as avian influenza virus, Escherichia coli, duck-derived avian bacillus, Staphylococcus, and Streptococcus from infected chickens. Among these, Escherichia coli has been identified as the primary pathogen due to its high detection rate.

[0003] Currently, pure traditional Chinese medicine preparations are becoming an alternative in the market. Their green and residue-free characteristics meet the needs of safe farming, but they have limitations such as slower onset of action and longer treatment courses. Oviductitis in laying hens is a significant issue restricting the healthy development of the industry; therefore, it is necessary to balance prevention and control effectiveness with food safety. Prioritizing the use of traditional Chinese medicine preparations, and dynamically adjusting medication strategies according to the laying stage and symptoms, can reduce farming costs and drug residue risks. Simultaneously, strengthening research on the mechanisms of action of functional nutrients and developing application technologies holds promise for opening up new pathways for the green prevention and control of oviductitis. Summary of the Invention

[0004] To address the aforementioned problems in the existing technology, this invention provides a traditional Chinese medicine composition for the prevention and treatment of oviductitis in chickens, its preparation method, and its application. The composition of this invention is mild in nature, has significant therapeutic effects, and the applied traditional Chinese medicine components are inexpensive and readily available, resulting in high cost-effectiveness. Furthermore, the composition of this invention has virtually no side effects and no risk of drug residues, conforming to green disease control practices.

[0005] To achieve the above-mentioned technical effects, the present invention adopts the following technical solution.

[0006] In a first aspect, the present invention provides a traditional Chinese medicine composition for treating oviductitis in chickens, the active ingredients of which include the following raw materials in parts by weight: Alisma plantago-aquatica 20-120 parts, Coix lacryma-jobi 20-120 parts, Dioscorea hypoglauca 10-40 parts, Prunus persica 10-30 parts, Ligusticum chuanxiong 10-40 parts, Achyranthes bidentata 15-50 parts, Glycyrrhiza uralensis 5-20 parts, and Alpinia oxyphylla 10-20 parts.

[0007] Furthermore, its active ingredients include the following composition in parts by weight: 30-100 parts of Alisma plantago-aquatica, 30-100 parts of Coix lacryma-jobi, 10-30 parts of Dioscorea hypoglauca, 10-30 parts of Prunus persica, 10-40 parts of Ligusticum chuanxiong, 15-50 parts of Achyranthes bidentata, 5-20 parts of Glycyrrhiza uralensis, and 10-20 parts of Alpinia oxyphylla.

[0008] Optional ingredients include: 40-100 parts of Alisma plantago-aquatica, 30-100 parts of Coix lacryma-jobi, 20-30 parts of Dioscorea hypoglauca, 15-30 parts of Prunus persica, 15-40 parts of Ligusticum chuanxiong, 15-40 parts of Achyranthes bidentata, 10-15 parts of Glycyrrhiza uralensis, and 10-20 parts of Alpinia oxyphylla.

[0009] Optionally, its active ingredients include the following composition in parts by weight: 60-63 parts of Alisma plantago-aquatica, 40-45 parts of Coix lacryma-jobi, 20-25 parts of Dioscorea hypoglauca, 20-23 parts of Prunus persica, 20-25 parts of Ligusticum chuanxiong, 28-33 parts of Achyranthes bidentata, 10-15 parts of Glycyrrhiza uralensis, and 15-18 parts of Alpinia oxyphylla.

[0010] Preferably, its active ingredients comprise the following composition in parts by weight: 60 parts Alisma plantago-aquatica, 40 parts Coix lacryma-jobi, 20 parts Dioscorea hypoglauca, 20 parts Prunus persica, 20 parts Ligusticum chuanxiong, 30 parts Achyranthes bidentata, 12 parts Glycyrrhiza uralensis, and 15 parts Alpinia oxyphylla.

[0011] Preferably, its active ingredients comprise the following composition in parts by weight: 30 parts Alisma plantago-aquatica, 100 parts Coix lacryma-jobi, 10 parts Dioscorea hypoglauca, 30 parts Prunus persica, 10 parts Ligusticum chuanxiong, 50 parts Achyranthes bidentata, 5 parts Glycyrrhiza uralensis, and 20 parts Alpinia oxyphylla.

[0012] Preferably, its active ingredients comprise the following composition in parts by weight: 100 parts Alisma plantago-aquatica, 30 parts Coix lacryma-jobi, 30 parts Dioscorea hypoglauca, 10 parts Prunus persica, 40 parts Ligusticum chuanxiong, 15 parts Achyranthes bidentata, 20 parts Glycyrrhiza uralensis, and 10 parts Alpinia oxyphylla.

[0013] Optionally, the dosage form of the traditional Chinese medicine composition may be any one of oral liquid, powder, spray, ointment, injection, or capsule.

[0014] In a preferred embodiment, the dosage form is an oral liquid or powder.

[0015] Optionally, the traditional Chinese medicine composition is an aqueous extract or a decoction extract.

[0016] Optionally, the amount of raw medicinal material in the oral liquid is 0.5~2g / mL, and preferably, the amount of raw medicinal material in the oral liquid is 1g / mL.

[0017] In a second aspect, the present invention provides a traditional Chinese medicine composition oral liquid, which includes the traditional Chinese medicine composition described in the first aspect.

[0018] Thirdly, the present invention provides a method for preparing a traditional Chinese medicine composition as described in the first aspect or an oral liquid of a traditional Chinese medicine composition as described in the second aspect, comprising taking each medicinal material in the weight proportions described in the first aspect, pulverizing, decocting, and concentrating to obtain the product.

[0019] Optionally, it includes the following steps: S1: Take each medicinal material in the weight proportions as described in the first aspect, crush and sieve them to obtain a mixture; S2: Boil the mixture described in S1; S3: Filter the Chinese herbs after decocting in S2 and decoct them again; S4: Combine the decoctions from S2 and S3, concentrate under reduced pressure until the raw drug content is 0.5~2g / mL, and set aside.

[0020] Preferably, in S1, the sieving is a 60-mesh pharmacopoeia sieve.

[0021] Preferably, in step S2, the decocting time is 0.5 to 2 hours, and more preferably 1.5 hours.

[0022] Preferably, in S2, the decoction solvent is water, wherein the amount of water used is 8 to 12 times the total weight of the medicinal materials, for example, 10 times.

[0023] Preferably, in step S3, the second decocting time is 0.5 to 2 hours, and more preferably 1.5 hours.

[0024] Preferably, in S3, the solvent for the second decoction is water, wherein the amount of water used is 8 to 12 times the total weight of the medicinal materials, for example, 10 times.

[0025] Preferably, in step S4, the decoctions from steps S2 and S3 are combined and concentrated under reduced pressure until the amount of raw medicinal material is 1 g / mL, for later use.

[0026] Fourthly, the present invention provides the use of the traditional Chinese medicine composition as described in the first aspect or the oral liquid of the traditional Chinese medicine composition as described in the second aspect in the preparation of drugs for the prevention, relief and / or treatment of oviductitis in chickens.

[0027] In this invention, when adding the pulverized drug to chicken feed, it is essential to ensure thorough and uniform mixing of the drug and feed. A mixer or similar equipment can be used for this purpose. When chickens exhibit symptoms of oviductitis or are at high risk of infection, a course of treatment consists of continuously feeding the medicated feed for 5-7 days. The course of treatment can be adjusted appropriately based on the severity of the condition and the treatment effect. For the use of a compound preparation, administration can be administered via drinking water. Dilute the compound preparation in the chickens' drinking water at a specific ratio, generally adding 1-10 mL of the compound preparation per liter of drinking water. Ensure the chickens have free access to the medicated water, and continue this for 5-7 days as one course of treatment. During treatment, the treatment plan can be adjusted promptly based on changes in the chickens' symptoms, such as egg production, egg quality, the chickens' mental state, and appetite.

[0028] The beneficial effects of the present invention are as follows: (1) The present invention provides a traditional Chinese medicine composition, which is mild in nature and can reduce the occurrence of bloody eggs, fecal eggs, soft-shelled eggs and sandy-shelled eggs in laying hens with oviductitis, improve the state of low spirits and poor appetite of sick chickens, and has significant curative effect. It can reduce the edema and inflammation of the oviduct of chickens, improve local blood circulation, promote tissue repair, and has a therapeutic effect on oviductitis of chickens. (2) The traditional Chinese medicine composition of the present invention is inexpensive, readily available, cost-effective, and has no risk of drug residues, which is in line with green prevention and control. (3) The preparation method of the traditional Chinese medicine composition of the present invention is simple and the traditional Chinese medicine composition obtained is convenient to use. Attached Figure Description

[0029] One or more embodiments are illustrated by way of example with reference to the accompanying drawings, and these illustrative examples are not intended to limit the embodiments. The term "illustrative" as used herein means "serving as an example, embodiment, or illustration." Any embodiment illustrated herein as "illustrative" is not necessarily to be construed as superior to or better than other embodiments.

[0030] Figure 1 It has an abnormal egg morphology; Figure 2 To test the egg production quality of chickens before administering the drug; Figure 3 To assess the egg production quality of experimental chickens after drug administration. Detailed Implementation

[0031] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0032] Furthermore, to better illustrate the present invention, numerous specific details are provided in the following detailed embodiments. Those skilled in the art should understand that the present invention can be practiced without certain specific details. In some embodiments, materials, elements, methods, and means well known to those skilled in the art are not described in detail in order to highlight the spirit of the invention.

[0033] The present invention relates to a traditional Chinese medicine composition for treating oviductitis in chickens, comprising the following components: Alisma plantago-aquatica, Coix lacryma-jobi, Dioscorea hypoglauca, Prunus persica, Ligusticum chuanxiong, Achyranthes bidentata, Glycyrrhiza uralensis, and Alpinia oxyphylla.

[0034] Among them, it promotes water drainage and moisture removal: Alisma plantago-aquatica: Sweet and cold in nature. It enters the kidney and bladder meridians. It can promote diuresis, eliminate dampness, and clear heat.

[0035] Job's tears: sweet, bland, and cool in nature. It enters the spleen, stomach, and lung meridians. It has the effects of promoting diuresis and eliminating dampness, strengthening the spleen and stopping diarrhea. On one hand, it assists Poria and Alisma in promoting diuresis; on the other hand, it strengthens the spleen to improve the chicken's digestive function, ensure nutrient absorption, and give the chicken enough energy to fight inflammation and promote the repair of oviduct tissue.

[0036] Clearing heat and promoting diuresis: Dioscorea hypoglauca: Bitter, neutral. It enters the liver, stomach, and bladder meridians. It has the effect of eliminating dampness and turbidity, clearing damp-heat in the lower burner. It can improve symptoms such as increased inflammatory secretions caused by damp-heat accumulation in the oviduct of chickens, helping to reduce abnormal secretions in the oviduct and alleviate inflammatory reactions.

[0037] Promotes blood circulation and removes blood stasis: Peach kernel: bitter, sweet, and neutral in nature. It enters the heart, liver, and large intestine meridians. It has the effect of promoting blood circulation and removing blood stasis. It can improve local blood circulation in the fallopian tubes, promote the dissipation of blood stasis, relieve congestion and bleeding symptoms of the fallopian tube mucosa, and is beneficial for the absorption of inflammation and tissue repair.

[0038] Chuanxiong (Ligusticum striatum): pungent, warm. It enters the liver, gallbladder, and pericardium meridians. It invigorates blood and promotes qi circulation. When combined with peach kernel, it enhances the effects of invigorating blood and removing blood stasis, ensuring smooth flow of qi and blood. Where there is free flow, there is no pain, thus alleviating pain caused by inflammation and blood stasis, and also helping to improve the function of the fallopian tubes.

[0039] Guide the drug downwards: Achyranthes bidentata: Sweet, slightly bitter, neutral in nature. It enters the liver and kidney meridians. It has the functions of removing blood stasis, promoting menstruation, and guiding blood downwards. It can guide other medicines downwards to the lower abdomen, directly reaching the affected area, allowing the medicine to act more effectively on the fallopian tubes, enhancing its efficacy. It also helps promote the discharge of stagnant blood and inflammatory secretions from the fallopian tubes.

[0040] Harmonizing other herbs: Licorice is sweet and neutral in nature. It enters the heart, lung, spleen, and stomach meridians. It can harmonize other herbs, moderate their properties, and promote synergistic effects among them, reducing adverse reactions of the drugs on the chickens. At the same time, licorice itself also has certain detoxifying and analgesic effects, which can help alleviate the chickens' discomfort symptoms.

[0041] Alpinia oxyphylla: warms the spleen and stops diarrhea, warms the kidneys and astringes, and improves eggshell quality.

[0042] This formula, through its effects of promoting diuresis and removing dampness, clearing heat and dampness, and promoting blood circulation and removing blood stasis, can reduce edema and inflammation of the oviduct in chickens, improve local blood circulation, and promote tissue repair, thus having a therapeutic effect on oviductitis in chickens.

[0043] Example 1 Alisma plantago-aquatica 60g, Coix lacryma-jobi 40g, Dioscorea hypoglauca 20g, Prunus persica 20g, Ligusticum chuanxiong 20g, Achyranthes bidentata 30g, Glycyrrhiza uralensis 12g, Alpinia oxyphylla 15g.

[0044] Example 2 Alisma plantago-aquatica 30g, Coix lacryma-jobi 100g, Dioscorea hypoglauca 10g, Prunus persica 30g, Ligusticum chuanxiong 10g, Achyranthes bidentata 50g, Glycyrrhiza uralensis 5g, Alpinia oxyphylla 20g.

[0045] Example 3 Alisma plantago-aquatica 100g, Coix lacryma-jobi 30g, Dioscorea hypoglauca 30g, Prunus persica 10g, Ligusticum chuanxiong 40g, Achyranthes bidentata 15g, Glycyrrhiza uralensis 20g, Alpinia oxyphylla 10g.

[0046] Comparative Example 1 Jidan Powder: 100g Polygonum cuspidatum, 80g Salvia miltiorrhiza, 60g Cuscuta chinensis, 60g Angelica sinensis, 60g Ligusticum chuanxiong, 60g Ostrea gigas, 50g Sanguisorba officinalis, 60g Cistanche deserticola, 20g Syzygium aromaticum, 50g Paeonia lactiflora.

[0047] Comparative Example 2 Yi Mu Sheng Hua San: Composed of 30g of motherwort, 24g of angelica, 9g of chuanxiong, 6g of peach kernel, 2g of prepared ginger, and 9g of prepared licorice.

[0048] Comparative Example 3 Scutellaria baicalensis 15g, Coptis chinensis 10g, Phellodendron chinense 15g, Gardenia jasminoides 10g, Pulsatilla chinensis 20g, Fraxinus chinensis 15g, Forsythia suspensa 10g, Glycyrrhiza uralensis 5g. Source: Traditional Chinese Veterinary Medicine Therapeutics (China Agriculture Press, 2008).

[0049] Comparative Example 4 Angelica sinensis 20g, Ligusticum chuanxiong 15g, Paeonia lactiflora 15g, Prunus persica 10g, Carthamus tinctorius 10g, Salvia miltiorrhiza 20g, Leonurus japonicus 10g, Glycyrrhiza uralensis 5g. Source: *Compilation of Modern Chinese Veterinary Clinical Experience* (Beijing Agricultural University Press, 2012).

[0050] Comparative Example 5 100 parts Atractylodes lancea, 60 parts Poria cocos, 50 parts Atractylodes macrocephala, 40 parts Polygonum aviculare, 40 parts Alisma plantago-aquatica, 50 parts Plantago asiatica, 40 parts Lepidium apetalum, 30 parts Gardenia jasminoides, 30 parts Rheum palmatum, 30 parts Magnolia officinalis, 30 parts Ligusticum chuanxiong, 40 parts Coptis chinensis, 40 parts Phellodendron chinense, and 20 parts Glycyrrhiza uralensis. Source: Patent Application No. 202210896746.X.

[0051] Comparative Example 6 Codonopsis pilosula 20 parts, Atractylodes macrocephala 20 parts, Angelica sinensis 30 parts, Ligusticum chuanxiong 20 parts, Astragalus membranaceus 20 parts, Acanthopanax senticosus 15 parts, Rehmannia glutinosa 20 parts, Cinnamomum cassia 20 parts, Prunus persica 10 parts, Carthamus tinctorius 20 parts, Agrimonia pilosa 20 parts. Source: Patent Application No.: 201410388046.5.

[0052] Comparative Example 7 Phellodendron bark 15g, Atractylodes lancea 15g, Citrus reticulata peel 10g, Magnolia officinalis 10g, Plantago asiatica seed 15g, Poria cocos 15g, Alisma plantago-aquatica 10g, Glycyrrhiza uralensis 5g. Source: Traditional Chinese Veterinary Medicine Formulas (Higher Education Press, 2009).

[0053] Comparative Example 8 Polyporus umbellatus 60g, Coix seed 40g, Plantago seed 20g, Peach kernel 20g, Ligusticum chuanxiong 20g, Achyranthes bidentata 30g, Licorice 12g, Alpinia oxyphylla 15g.

[0054] Comparative Example 9 Poria cocos 60g, Kochia scoparia 40g, Talcum 20g, Prunus persica 20g, Corydalis yanhusuo 20g, Achyranthes bidentata 30g, Glycyrrhiza uralensis 12g, Psoralea corylifolia 15g.

[0055] Comparative Example 10 Alisma plantago-aquatica 15g, Coix lacryma-jobi 40g, Dioscorea hypoglauca 20g, Prunus persica 200g, Ligusticum chuanxiong 20g, Achyranthes bidentata 10g, Glycyrrhiza uralensis 12g, Alpinia oxyphylla 60g.

[0056] Comparative Example 11 Alisma plantago-aquatica 10g, Coix lacryma-jobi 40g, Dioscorea hypoglauca 50g, Prunus persica 80g, Ligusticum chuanxiong 20g, Achyranthes bidentata 30g, Glycyrrhiza uralensis 60g, Alpinia oxyphylla 70g.

[0057] Formulation preparation example 1. Powder preparation The herbal compositions of the above embodiments and comparative examples can be prepared into powder. The specific preparation method is as follows: each herb is pulverized and passed through a 60-mesh pharmacopoeia sieve, then mixed and used in feed. In this invention, comparative examples 1-7 were all prepared into powder using the above method.

[0058] During the challenge, mix 5g of the drug with each kilogram of feed and use it for 7 consecutive days.

[0059] 2. Preparation of oral liquid The traditional Chinese medicine compositions of the above embodiments and comparative examples 8-11 can be prepared into oral liquids. The specific preparation method is as follows: Take the weight parts of each medicinal material, add 10 times the amount of water, decoct twice, 1.5 hours each time, combine the two decoctions, and concentrate under reduced pressure to a raw drug content of 1g / mL.

[0060] Application Example 1 1. Experimental Objective By establishing a model of oviductitis in laying hens, and then treating selected reserve samples, the statistical data analysis results were used to verify the efficacy of the herbal composition of this invention in preventing and treating oviductitis in laying hens.

[0061] 2. Test materials Experimental animals: 480-day-old laying hens. Strand: Large Crested White laying hens, purchased from Kangxin Breeding Farm, Pingyuan County, Shandong Province.

[0062] Feed: Gaotang Haiding Laying Hen Compound Feed 324, 40kg / bag, tap water.

[0063] Experimental instruments: dropper, syringe, gavage needle, surgical scissors, etc.

[0064] Test reagent: Infection solution (containing Escherichia coli O) 78 ).

[0065] Escherichia coli O 78 Bacterial culture: Picking out Escherichia coli O 78 Single colonies were plated onto nutrient broth and incubated in a constant temperature incubator at 37°C for 20 hours. The bacterial culture contained *E. coli* O0. 78 The concentration is 1.8 × 10⁻⁶. 9 CFU / mL.

[0066] 3. Experimental grouping and treatment Each of the traditional Chinese medicine compositions in the examples and comparative examples was prepared into powder according to the preparation example above. The bacterial solution was then challenged into each test group according to the grouping and treatment method in Table 1.

[0067] Table 1. Treatment methods for each experimental group ; 4. Modeling methods Experimental animals: Old laying hens, which were pre-adapted to the experiment by being fed a basal diet (Gaotang Haiding Laying Hen Compound Feed 324) for 10 days.

[0068] The experimental chickens were housed in separate cages, divided into 16 groups. Based on the number of eggs laid during the acclimatization period, the original cages were broken up to regroup the chickens, with the goal of balancing egg production in each group.

[0069] Modeling method: Remove the chicken from the cage, first fix its two legs so that its back faces you, cloaca is facing upwards, and head is downwards. Then, place your left thumb and forefinger in a V-shape across the soft parts on both sides of the cloaca and gently press down until the oviduct opening is exposed. Apply the modeling reagent (i.e., a concentration of 1.8 × 10⁻⁶) to the chicken. 9 CFU / mL E. coli O 78 The bacterial solution can be injected into the fallopian tube 2-3 cm deep.

[0070] The medication was administered the day before modeling. From the first day of modeling, the egg production and abnormal eggs of the experimental chickens were observed daily. Abnormal eggs included blood eggs, fecal eggs, soft-shelled eggs, and sandy-shelled eggs. The mental state, appetite, morbidity, and mortality of the experimental chickens were also observed and recorded. The chickens were observed for 7 consecutive days.

[0071] 5. Results Twenty-four hours after the virus challenge, the laying hens in the experimental challenge groups (i.e., Examples 1-3, Comparative Examples 1-11 and the model group) all showed varying degrees of lethargy, loss of appetite, reduced feed intake, decreased egg production rate, bloodstains on the eggshells, and yellowish-white secretions contaminating the feathers near the cloaca. Most of the laying hens after the virus challenge showed difficulty in laying eggs.

[0072] Egg production: The total number of eggs produced in each experimental group is shown in Table 2.

[0073] Table 2. Daily egg production of chickens in each experimental group (eggs / day) ; Note: P < 0.05 indicates a significant difference from the model group.

[0074] The results showed that Example 1 had the best effect in preventing and treating the decline in egg production caused by oviductitis in laying hens, and was significantly different from the model group (P < 0.05). Examples 2-3 also had significant effects, while the other groups were not significantly different from the model group (P > 0.05).

[0075] Eggshell condition: Total number of abnormal eggs in each group during the experiment (in this experiment, eggs with bloodstains, soft-shelled eggs, rough-shelled eggs, and dirty or cracked eggs were counted as abnormal eggs; see [link to relevant documentation]). Figure 1 The results are shown in Table 3.

[0076] Table 3. Abnormal Eggs in Each Group of Chickens (number of eggs / piece) ; Results: By comparing the number of eggs laid, the number of abnormal eggs, and the mortality rate of laying hens in each experimental group, it was found that Examples 1-3 had significant effects compared to other existing technical solutions. When the weight proportions were within the range, the effects were significant. However, the effects of Examples 1-3 were not as good as Examples 1-3 after replacing the medicinal materials with the same efficacy, and the effects were not as good as Examples 1-3 when the ratio of the medicinal materials in the formulation exceeded the range. Based on the analysis of the above experimental results, among the above examples, Example 1 has a more prominent effect on the prevention and treatment of oviductitis in laying hens.

[0077] Inflammation of the oviduct in chickens can cause eggs to be stained with blood, have soft shells, have rough shells, or have dirty, broken shells. Edema of the oviduct and poor blood circulation can lead to a decrease in egg production. Therefore, based on the above data, it can be shown that the traditional Chinese medicine composition of the present invention can alleviate edema and inflammation of the oviduct in chickens, improve local blood circulation, and promote tissue repair.

[0078] Application Example 2: Clinical Results 1. Experimental Materials and Methods 1.1 Test Materials 1.1.1 Test Drug The oral liquid prepared from the traditional Chinese medicine composition in Example 1 was provided by Beijing Shengtai Technology Co., Ltd.

[0079] 1.1.2. Experimental Animals 360-day-old Hy-Line Brown commercial laying hens.

[0080] 1.1.3 Test Site A chicken farm in Gaotang, Shandong.

[0081] 1.2 Test Methods A chicken coop house contained 8700 Hy-Line Brown commercial laying hens, 360 days old, with clinical signs of salpingitis. The traditional Chinese medicine composition from Example 1 was prepared into an oral liquid according to the preparation method described above. Clinical indicators were observed before and after medication. The method of use is shown in Table 4.

[0082] Table 4. Usage Instructions

[0083] 1.3 Data Collection and Analysis Observe and record changes in eggshell color and egg production rate in the flock, and the results are as follows: Figure 2 and Figure 3 As shown.

[0084] Among them, based on the breed identification of laying hens in the flock, when laying hens lay white-shelled eggs and dirty eggs, they can be identified as diseased laying hens (single dose of oviductitis), and the eggs they lay are recorded as abnormal eggs. Egg production rate = number of eggs produced / total number of chickens × 100%.

[0085] Depend on Figure 2 and Figure 3 As can be seen, after 5 days of use in Example 1, the percentage of white-shelled eggs decreased from 9.7% to 1.3%, and the percentage of dirty eggs decreased from 10.3% to 1.5%. The egg production rate increased from 90.2% before administration to 95.6%, showing a significant effect on increasing egg production. Furthermore, the eggs produced after administration had smooth surfaces and a bright color.

[0086] Results: This experiment verified that Example 1 is safe and effective in treating oviductitis in commercial laying hens.

[0087] In summary, Examples 1-3 have a preventive and therapeutic effect on oviductitis in laying hens, and have a more prominent therapeutic effect compared with existing preventive and therapeutic drugs.

[0088] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A traditional Chinese medicine composition for the prevention and treatment of oviductitis in chickens, the active ingredients of which are made from the following raw materials in parts by weight: Alisma plantago-aquatica 20-120 parts, Coix lacryma-jobi 20-120 parts, Dioscorea hypoglauca 10-40 parts, Prunus persica 10-30 parts, Ligusticum chuanxiong 10-40 parts, Achyranthes bidentata 15-50 parts, Glycyrrhiza uralensis 5-20 parts, and Alpinia oxyphylla 10-20 parts.

2. The traditional Chinese medicine composition according to claim 1, characterized in that, Its active ingredients are made from the following raw materials in parts by weight: Alisma plantago-aquatica 30-100 parts, Coix lacryma-jobi 30-100 parts, Dioscorea hypoglauca 10-30 parts, Prunus persica 10-30 parts, Ligusticum chuanxiong 10-40 parts, Achyranthes bidentata 15-50 parts, Glycyrrhiza uralensis 5-20 parts, and Alpinia oxyphylla 10-20 parts.

3. The traditional Chinese medicine composition according to claim 1 or 2, characterized in that, Its active ingredients are made from the following raw materials in parts by weight: Alisma plantago-aquatica 60-63 parts, Coix lacryma-jobi 40-45 parts, Dioscorea hypoglauca 20-25 parts, Prunus persica 20-23 parts, Ligusticum chuanxiong 20-25 parts, Achyranthes bidentata 28-33 parts, Glycyrrhiza uralensis 10-15 parts, and Alpinia oxyphylla 15-18 parts.

4. The traditional Chinese medicine composition according to claim 1, characterized in that, Its active ingredient is made from any one of the following raw materials in parts by weight: (1) Its active ingredients are made from the following raw materials in parts by weight: 60 parts of Alisma plantago-aquatica, 40 parts of Coix lacryma-jobi, 20 parts of Dioscorea hypoglauca, 20 parts of Prunus persica, 20 parts of Ligusticum chuanxiong, 30 parts of Achyranthes bidentata, 12 parts of Glycyrrhiza uralensis, and 15 parts of Alpinia oxyphylla. (2) Its active ingredients are made from the following raw materials in parts by weight: 30 parts of Alisma plantago-aquatica, 100 parts of Coix lacryma-jobi, 10 parts of Dioscorea hypoglauca, 30 parts of Prunus persica, 10 parts of Ligusticum chuanxiong, 50 parts of Achyranthes bidentata, 5 parts of Glycyrrhiza uralensis, and 20 parts of Alpinia oxyphylla. (3) Its active ingredients are made from the following raw materials in parts by weight: 100 parts of Alisma plantago-aquatica, 30 parts of Coix lacryma-jobi, 30 parts of Dioscorea hypoglauca, 10 parts of Prunus persica, 40 parts of Ligusticum chuanxiong, 15 parts of Achyranthes bidentata, 20 parts of Glycyrrhiza uralensis, and 10 parts of Alpinia oxyphylla.

5. The traditional Chinese medicine composition according to claim 1, characterized in that, The dosage form of the traditional Chinese medicine composition is any one of oral liquid, powder, spray, ointment, injection, or capsule.

6. The traditional Chinese medicine composition according to claim 1, characterized in that, The traditional Chinese medicine composition is an aqueous extract or a decoction extract.

7. The traditional Chinese medicine composition according to claim 5, characterized in that, The amount of raw medicinal material in the oral liquid is 0.5~2g / mL.

8. The traditional Chinese medicine composition according to claim 7, characterized in that, The amount of raw medicinal material in the oral liquid is 1 g / mL.

9. A traditional Chinese medicine composition oral liquid, wherein the active ingredient is made from the traditional Chinese medicine composition as described in any one of claims 1-8.

10. A method for preparing a traditional Chinese medicine composition as described in any one of claims 1-8 or an oral liquid of a traditional Chinese medicine composition as described in claim 9, comprising taking each medicinal material in the weight proportions described in any one of claims 1-4, pulverizing, decocting, and concentrating to obtain the product.

11. The preparation method according to claim 10, characterized in that, It includes the following steps: S1: Take each medicinal material according to the stated weight proportions, crush and sieve them to obtain a mixture; S2: Boil the mixture described in S1; S3: Filter the Chinese herbs after decocting in S2 and decoct them again; S4: Combine the decoctions from S2 and S3, concentrate under reduced pressure until the raw drug content is 0.5~2g / mL, and set aside.

12. The use of a traditional Chinese medicine composition as described in any one of claims 1-8 or an oral liquid of a traditional Chinese medicine composition as described in claim 9 in the preparation of a drug for preventing oviductitis in chickens.

13. The use of a traditional Chinese medicine composition as described in any one of claims 1-8 or an oral liquid of a traditional Chinese medicine composition as described in claim 9 in the preparation of a drug for relieving oviductitis in chickens.

14. The use of a traditional Chinese medicine composition as described in any one of claims 1-8 or an oral liquid of a traditional Chinese medicine composition as described in claim 9 in the preparation of a drug for treating chicken oviductitis.

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