Paenibacillus yunnanensis, microbial inoculant containing the same and use

Abstract

The present application relates to the field of microorganisms and applications, and provides a Paenibacillus elyakovii, a microbial inoculant containing the same and purposes. Paenibacillus ehimensis ​ The strain KY1075 of the Paenibacillus elyakovii provided in the present application is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.35207. The strain is a multifunctional biocontrol microbial strain isolated and screened from soil, has a wide bactericidal spectrum and strong activity, shows significant and specific bactericidal activity on the whole pathogenic bacteria of soybean root rot, and shows excellent inhibition effect on other various plant pathogenic fungi and bacteria, and has a good application prospect.

Description

Technical Field

[0001] This invention relates to the field of microorganisms and their applications, specifically to a strain of Bacillus erythropoietinus, microbial agents containing it, and their applications. Background Technology

[0002] Soybeans, as one of the major grain, oil, and feed crops, provide over 60% of edible vegetable oil and over 70% of protein feed ingredients, supporting downstream industries such as food processing and livestock breeding. Their fundamental and irreplaceable role in the agricultural system is evident (Lu Mengnan et al., 2025). However, a major challenge exists in soybean production: soybean root rot. Soybean root rot There is still no effective solution for soybean root rot (CHANDRA S et al., 2022). Soybean root rot is a global soil-borne disease that has spread to soybean-producing areas worldwide since its first report in the United States in 1917, posing a persistent threat to the soybean industry (Liang Wenwei et al., 2025). The disease can occur throughout the soybean growth cycle, primarily infecting the roots, which provide water and nutrients for plant growth. Damage to this crucial part triggers a chain reaction of damage, such as yellowing leaves, poor root development, reduced number of root nodules, stunted above-ground parts, and a sharp decrease in pod production. In severe cases, the entire plant withers and dies (Xu Jiefeng et al., 2022). In terms of yield loss, affected fields typically experience yield reductions of 10%–30%, with severely affected fields experiencing reductions exceeding 60%, and in extreme cases, even total crop failure, resulting in significant economic losses (Chen Xijun et al., 2023). The core issue in controlling soybean root rot in the field lies in the mismatch between the complexity of pathogenic complex infection and the singularity of field symptoms. The pathogens causing the disease in the field include multiple fungi such as Fusarium, Phytophthora, and Pythium, with most fields containing 2-3 different pathogens. However, the plants ultimately exhibit only the single symptom of root rot and above-ground wilting. Therefore, it is difficult to accurately identify the pathogenic group based on appearance alone, and applying a single agent or measure cannot cover all pathogens, significantly reducing the effectiveness of control. Furthermore, the pathogens can spread through multiple routes, including soil, seeds, and irrigation water, and can survive in the soil for 3-5 years, forming a stable source of infection, further exacerbating the difficulty of control (HALE B et al., 2023).

[0003] Soybean root rot is caused by Phytophthora soybeanum (… Phytophthora sojae Fusarium ( ) Fusarium spp. ), Pythium ( Pythium spp. Rhizoctonia solani ( ), Rhizoctonia solani Rhizoctonia solani This is caused by one or more combined infections of Phytophthora in soybean. Phytophthora belongs to the genus Phytophthora (…). PhytophthoraMorphologically similar to fungi, but unlike true fungi, its cell wall contains cellulose instead of chitin. As a soil-borne oomycete, it can overwinter in soil or diseased plant debris and can spread over long distances by adhering to the seed surface. This pathogen can infect all parts of soybean growth, leading to disease throughout the entire growth period (Zhang Baoqiang, 2010). The most common pathogen in the Fusarium genus is *Fusarium oxysporum* (…). F. oxysporum *Fusarium solani* is the dominant pathogenic group, while *Fusarium solanum* and others are minor pathogenic groups. *Fusarium* exhibits strong environmental adaptability, surviving adverse conditions in the soil through dormant hyphae or sclerotia, with dormancy lasting up to 5 years. It primarily attacks the vascular system of the soybean plant cortex, disrupting nutrient and water transport pathways. Throughout the infection cycle, *Fusarium* continuously secretes toxins, severely harming soybean health. This pathogen mainly spreads through contaminated soil and diseased plant debris (such as residual roots and stems) in the field, easily leading to pathogen accumulation in continuously cropped fields. Its damage is not limited to the vegetative growth stage but also infects reproductive organs, causing seed rot, reduced germination rates, and brown spots on stems that gradually spread, ultimately resulting in a severe decline in pod yield and quality (Sun Yangping, 2025; Cheng Rong et al., 2016; DORRANCE A E., 2018; Zhou Jiangming, 2024). Rhizoctonia solani overwinters primarily in the soil as sclerotia or as mycelium on diseased rice straw and other host debris. It can spread through irrigation water, infecting plants and causing diseases including damping-off, root rot, and brown spot. Its host range is wide, including but not limited to rice, wheat, corn, potatoes, broad beans, peanuts, and cotton, often leading to reduced yield and quality. In soybeans, due to its root damage, it can cause stunted growth and thinner rootstocks, thus affecting pod number and grain weight (Dorrance AE et al., 2003; Senapati M et al., 2022; Chen Xingke, 2024). *Pythium* genus (… Pythium After infecting soybeans, *Pythium* fungi cause root rot, mainly manifested as brown, water-soaked lesions on the roots, weak and softened stems, yellowing and wilting leaves, ultimately leading to plant collapse and death. In the seedling stage, it can also cause rotting before emergence and sudden collapse after emergence. Under high temperature and humidity, white, cottony, oily mycelium appears on the affected area and surrounding soil surface; under dry conditions, diseased plants show slow growth, yellowing, and root rot. Many species of *Pythium* fungi cause soybean root rot. Li et al. (2019) identified eight species causing soybean root rot, and new species have been continuously discovered in recent years (Kumar S et al., 2022). The dominant fungal species vary in different production areas.

[0004] Currently, the main methods for controlling soybean root rot include chemical pesticides, disease-resistant breeding, agricultural measures, and biological methods. While chemical pesticides have significant short-term control effects on soybean root rot, their long-term use has led to a series of serious problems. First, the effect of using a single chemical pesticide to control root rot symptoms is often poor. Second, long-term application of chemical pesticides not only leads to drug resistance in pathogens, making subsequent control much more difficult, but also causes soil pollution, harms beneficial organisms, and disrupts the ecological balance of farmland (Dong Jiwei et al., 2024). Disease-resistant breeding is an economical and effective means of controlling soil-borne diseases. However, there are currently no soybean varieties that are immune or highly resistant to soybean root rot. Existing resistant varieties only possess a certain degree of resistance and cannot completely solve the disease problem. Moreover, soybean disease resistance has shortcomings. While non-race-specific resistance can cope with multiple races of the same disease, the resistance is relatively weak. Race-specific resistance is only for specific diseases and is difficult to adapt to the variation of pathogens. At the same time, the selection of resistant varieties needs to be combined with the planting area. Different production areas have different applicable varieties, which further increases the difficulty of applying disease-resistant breeding (Yang Liu, 2022; Jiang Chao, 2025). Agricultural control of soybean root rot is centered on crop rotation and deep plowing. Although it can play a certain role in control by disrupting the life cycle of pathogens and improving the soil environment, its practical application is obviously limited. Crop rotation faces challenges such as a limited number of suitable crops, a long cycle (usually 3-5 years), and high risks (susceptibility to disease under extreme weather conditions and complex crop combinations). Deep tillage relies on large agricultural machinery, is costly, and cannot completely eliminate deep-seated pathogens; some pathogens can still harm soybean roots after tilling, resulting in limited control effects (Ye Wenwu et al., 2020; Neupane A et al., 2021). Biological control, as an environmentally friendly measure, reduces the use of chemical pesticides and maintains ecological balance through natural enemy insects, microorganisms, and plant-derived pesticides, and is receiving increasing attention. Microbial technology, in particular, is favored. Current research has confirmed that microorganisms such as Trichoderma (Shao Hongtao et al., 2004), Bacillus (Guo Rongjun et al., 2010), Pseudomonas (Gao Tongguo et al., 2015), and Streptomyces (Zhu Zhiyan et al., 2019) can effectively control soybean root rot.

[0005] Therefore, it remains necessary to continue screening and developing highly efficient biocontrol microbial strains with broad-spectrum control over all pathogens causing soybean root rot, and to establish green and sustainable biological control methods.

[0006] Based on this, the present invention is proposed. Summary of the Invention

[0007] This invention aims to at least partially address the technical problems existing in the prior art. To this end, this invention provides a strain of *Bacillus erythropoietinus* (…). Paenibacillus ehimensis ) strain KY1075, microbial agents containing it and their uses.

[0008] The present invention adopts the following technical solution: In a first aspect, the present invention provides a strain of *Bacillus erythropoietinus* (… Paenibacillus ehimensis The strain KY1075 is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 35207, and the deposit date is July 11, 2025.

[0009] In one embodiment, the 16S rDNA sequence of the strain KY1075 is shown in SEQ ID NO: 1.

[0010] This invention focuses on obtaining a highly efficient biocontrol microbial strain capable of broadly controlling all pathogens causing soybean root rot. Through high-throughput screening, a multifunctional biocontrol microorganism, KY1075, was obtained, exhibiting broad-spectrum resistance to plant pathogenic fungi, plant pathogenic bacteria, and specific control over all pathogens causing soybean root rot. 16S rDNA sequencing analysis revealed that this strain is similar to *Bacillus ehime* (…). Paenibacillus ehimensis It exhibits high homology. Activity tests were conducted in four culture media: R2A, PDA, LB, and NA. The results showed that KY1075 not only demonstrated significant specific fungicidal activity against all pathogenic fungi of soybean root rot, but also exhibited excellent broad-spectrum inhibitory effects against 17 plant pathogenic fungi and 6 plant pathogenic bacteria. Comparison with commercial strains *Bacillus polymyxa* and *Bacillus belye* revealed that KY1075 showed superior inhibitory activity against multiple plant pathogenic fungi and bacteria in all four culture media compared to these two commercial strains.

[0011] In a second aspect, the present invention provides a microbial inoculant containing the aforementioned *Bacillus erythropoietinus* (…). Paenibacillus ehimensis ) strain KY1075, or containing the aforementioned Bacillus erythropoietinus ( Paenibacillus ehimensis Fermentation products obtained from the fermentation of strain KY1075.

[0012] In one embodiment, the microbial agent contains the cells, fermentation broth, bacterial suspension, and / or fermentation supernatant of the *Bacillus erythropoietinus* strain KY1075.

[0013] In one embodiment, the fermentation includes processing *Bacillus erythropoietinus* (…). Paenibacillus from Ehime The strain KY1075 was fermented at 25℃-35℃, pH 7.0-7.5, and under aeration conditions.

[0014] In one embodiment, the microbial agent is a solid or liquid agent; when the microbial agent is a solid agent, the *Bacillus erythropoietinus* (Ehime-like Bacillus) is present in the agent. Paenibacillus ehimensis The effective viable count of strain KY1075 is at least 1×10⁻⁶.7 CFU / g; When the microbial agent is a liquid agent, the effective viable count of the *Bacillus erythropoietinus* KY1075 strain in the microbial agent is at least 1 × 10⁻⁶. 7 CFU / mL.

[0015] In one embodiment, the microbial agent further includes a carrier or excipient.

[0016] In a third aspect, the present invention provides the aforementioned *Ehime-like Bacillus* (… Paenibacillus ehimensis The use of strain KY1075 or the aforementioned microbial agents in the prevention and control of plant diseases.

[0017] In one implementation, the plant disease is a soil-borne disease or a surface disease.

[0018] In one embodiment, the plant disease includes one or more of the following: soybean root rot, peanut white mold, cotton wilt, watermelon wilt, banana wilt, wheat root rot, rice sheath blight, gray mold, wheat sheath blight, apple leaf spot, tomato early blight, pear black spot, bacterial wilt, white leaf blight, soft rot, pepper scab, and cucumber angular leaf spot.

[0019] In one embodiment, the soybean root rot is caused by Phytophthora soybeanum (… Phytophthora sojae Fusarium ( ) Fusarium spp. ), Pythium ( Pythium spp. ) and Rhizoctonia solani ( Rhizoctonia solani It is caused by a single infection or multiple combined infections in one of the following.

[0020] In a fourth aspect, the present invention provides the aforementioned *Ehime-like Bacillus* (… Paenibacillus ehimensis The use of strain KY1075 or the aforementioned microbial agents in the fight against plant pathogens.

[0021] In one embodiment, the plant pathogen includes Phytophthora soybeanum (… Phytophthora sojae Fusarium solani () Fusarium spp. ), Pythium ( Pythium spp. ) or Rhizoctonia solani ( Rhizoctonia solani Ralstonia solanacearum (Ralstonia solanacearum) Ralstonia solanacearum Xanthomonas oryzae, a pathogenic strain of rice ( Xanthomonas rice pv. rice Erwinia carotene ( ), Erwinia carotovora Xanthomonas aeruginosa, a pathogenic species of pepper ( Xanthomonas campestris pv. bladder ), Pseudomonas syringae pathogenic strain in cucumber ( Pseudomonas syringae pv. lachrymans ) and clusters of pantothenic bacteria ( Pantoea agglomerans ).

[0022] In a fifth aspect, the present invention provides a method for preventing and controlling plant diseases, the method comprising administering the aforementioned *Bacillus erythropoietinus* (… Paenibacillus ehimensis The strain KY1075 or the aforementioned microbial inoculant was applied to the plant.

[0023] In one embodiment, the method includes administering the aforementioned *Bacillus erythropoietinus* (…). Paenibacillus from Ehime The strain KY1075 or the aforementioned microbial agent may be applied to the seeds or leaves of the plant, or to the roots of the plant.

[0024] In one embodiment, the plant includes crops; preferably, the crop is one or more of soybeans, peanuts, cotton, watermelon, bananas, tomatoes, wheat, rice, apples, pears, peppers, and cucumbers. More preferably, the plant is soybeans.

[0025] Preservation information: The Ehime-like Bacillus provided in this application ( Paenibacillus ehimensis The strain, named KY1075, is deposited at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences. The deposit date is July 11, 2025, and the accession number is CGMCC NO. 35207. It was confirmed as a viable strain by the collection center on July 11, 2025.

[0026] The beneficial effects of this invention are as follows: The strain KY1075 or microbial agents containing it provided by this invention have a highly efficient and broad-spectrum anti-plant pathogen effect. It not only shows significant specific bactericidal activity against all pathogens of soybean root rot, but also exhibits excellent broad-spectrum inhibitory effects on a variety of other plants. It can be used for comprehensive prevention and control of a variety of above-ground and underground soil-borne diseases of plants, and has a high control effect, showing great application prospects in plant disease control.

[0027] This invention provides a green and sustainable method for controlling plant diseases, breaking through the bottlenecks of current soybean root rot control methods, which rely heavily on single-target treatments and chemical agents. This provides crucial technical support for the healthy and stable development of the soybean industry. Furthermore, the strain of this invention possesses broad-spectrum inhibitory capabilities against multiple major plant pathogenic fungi and bacteria, achieving "one strain, multiple defenses," further expanding the application scenarios and value of the results and providing new microbial resources for the green management of multiple crop diseases. Attached Figure Description

[0028] The above and / or additional aspects and advantages of the present invention will become apparent and readily understood from the description of the embodiments taken in conjunction with the following drawings, in which: Figure 1The figure shows microorganisms with antifungal activity against Phytophthora soybean, obtained through high-throughput screening. The boxes in the figure schematically show microorganisms that produce inhibition zones with a radius of 2 mm. Figure 2 Microorganisms with activity against pathogens associated with soybean root rot are shown. The two microorganisms shown in the figure can produce inhibition zones, and the radius of the inhibition zones is ≥1 mm. Figure 3 The condition of soybean plants 10 days after artificial re-inoculation with pathogenic bacteria D6 is shown. Figure 4 The morphology of strain KY1075 as observed under a microscope (100x oil immersion) is shown; Figure 5 The results of broad-spectrum fungicidal activity tests (fungal) of KY1075 and commercial strains (Bacillus polymyxa and Bacillus belys) in four culture media are shown. From top to bottom, the figures show the four pathogens of soybean root rot: Phytophthora soybeanis, Fusarium soybeanis, Rhizoctonia solani, and Pseudomonas aeruginosa. Figure 6 The results of the broad-spectrum fungicidal activity test (fungal) of KY1075 and commercial strains (Bacillus polymyxa and Bacillus belye) in four culture media are shown. From top to bottom in the figure, the pathogens of peanut white mold, cotton wilt and watermelon wilt are shown. Figure 7 The results of the broad-spectrum bactericidal activity test (bacterial) of KY1075 and commercial strains are shown. The figure shows the pathogens of pathogenic bacteria D6, bacterial wilt, bacterial leaf blight, soft rot, cucumber angular leaf spot, and pepper scab, respectively. Detailed Implementation

[0029] The embodiments of the present invention are described in detail below.

[0030] This invention presents a newly isolated strain of *Bacillus erythropoietinus* (Ehime-like Bacillus). Paenibacillus ehimensis Strain KY1075. This strain KY1075 is deposited at the China General Microbiological Culture Collection Center, with accession number CGMCC No. 35207. Soybean root rot ( Soybean root rotSoybean root rot, also known as soybean root rot, is notoriously difficult to control. This disease is typically caused by different pathogens. These pathogens exhibit significant differences in their infection pathways and pathogenic mechanisms—for example, *Phytophthora* primarily infects the vascular bundles of roots through zoospores, while *Fusarium* overwinters in the soil as mycelium or chlamydospores, damaging root tissue. Furthermore, root rot caused by multiple infections often presents with uniform symptoms—root rot, stunted growth, and yellowing and wilting of leaves are the main characteristics. The appearance of diseased plants in the field alone cannot accurately determine which pathogen(s) are responsible, making it difficult to select targeted control agents or measures, further increasing the difficulty of controlling soybean root rot. To address this industry challenge, the inventors' team systematically collected multiple typical diseased plant samples from soybean-producing fields in Dayangshu Town, Oroqen Autonomous Banner, Hulunbuir City, Inner Mongolia Autonomous Region, and conducted a comprehensive analysis of the pathogenic microorganisms in the samples. Furthermore, high-throughput screening was conducted using pathogenic bacteria as indicator bacteria to obtain 167 microorganisms effective against Phytophthora infestans, the pathogen causing soybean root rot. Based on this, 10 strains with fungicidal activity against multiple soybean root rot pathogens were further screened. From these strains, strain KY1075 was selected as the best performing strain. KY1075 is a multifunctional biocontrol microorganism with broad-spectrum resistance to plant pathogenic fungi / bacteria and specific control over all pathogens causing soybean root rot, showing great application potential in the development of the soybean industry and the control of soil-borne diseases. Based on the 16S DNA sequence determination, and through sequence alignment with the 16S RNA database (Chun's Lab) recognized by the International Commission on Bacteriology, combined with literature analysis, the taxonomic position of the target microorganism was determined (Yoon, SH, Ha, SM, Kwon, S., Lim, J., Kim, Y., Seo, H. and Chun, J. (2017). Introducing EzBioCloud: A taxonomically united database of 16S rRNA and whole genome assemblies. Int J Syst Evol Microbiol. 67:1613-1617). The alignment results confirmed that the strain was identified as *Bacillus ehimeensis*. Paenibacillus ehimensis Ehime-like Bacillus ( Paenibacillus ehimensis This strain was the first functional strain isolated from soil in Ehime Prefecture, Japan (Jung-Sook L et al., 2004). Currently, there are no reports on the use of *Bacillus erythropoietinus* in the control of soybean root rot. It should be noted that the strains screened in this invention are from the same genus... Paenibacillus ehimensis The four strains showed significant differences in bactericidal spectrum and bactericidal activity, with KY1075 exhibiting the broadest bactericidal spectrum and the strongest activity.

[0031] Furthermore, the present invention provides a microbial inoculant containing the aforementioned *Bacillus erythropoietinus* (…). Paenibacillus ehimensis ) strain KY1075 or Ehime-like Bacillus ( Paenibacillus ehimensis The microbial agent contains the aforementioned *Bacillus erythropoietinus* strain KY1075 as the main active ingredient. Specifically, the culture of strain KY1075 includes any one of fermentation broth, fermentation broth precipitate, fermentation broth supernatant, and fermentation broth extract.

[0032] In one embodiment, the microbial agent is a solid or liquid agent; the microbial agent contains the *Bacillus erythropoietinus* (…). Paenibacillus ehimensis The effective viable count of strain KY1075 is at least 1×10⁻⁶. 7 CFU / g or 1×10 7 CFU / mL. Specifically, the *Bacillus erythropoietinus* contained in the microbial agent (… Paenibacillus ehimensis The effective viable count of strain KY1075 is 10. 8 cfu·mL -1 10 9 cfu·mL -1 10 10 cfu·mL -1 , or 10 8 cfu·g -1 10 9 cfu·g -1 10 10 cfu·mL -1 .

[0033] In one specific embodiment, the microbial agent may also contain commonly used carriers or excipients, such as solid or liquid carriers, for example, bentonite, calcium carbonate, zeolite, starch; or vegetable oil, mineral oil, and water. In one specific embodiment, the microbial agent can be prepared into a bio-fertilizer with antagonistic properties against soybean root rot pathogens and / or other plant pathogens.

[0034] In one embodiment, the present invention provides the aforementioned *Ehime-like Bacillus* (… Paenibacillus from Ehime The use of strain KY1075 or the aforementioned microbial agents in the control of plant diseases. On one hand, the aforementioned *Bacillus erythropoietinus* (… Paenibacillus ehimensis Strain KY1075 or the aforementioned microbial inoculants can be used to control soybean root rot. Soybean root rot is caused by one or more of Phytophthora soybeani, Fusarium, Pythium, and Rhizoctonia solani. In particular, the *Bacillus erythropoietinus* strain of the present invention (…) Paenibacillus ehimensisStrain KY1075 or the aforementioned microbial agents can effectively prevent soybean root rot caused by complex infection of multiple pathogens. On the other hand, the aforementioned *Bacillus erythropoietinus* (… Paenibacillus ehimensis Strain KY1075 or the aforementioned microbial agents can be used to control peanut white mold, cotton wilt, watermelon wilt, banana wilt, wheat root rot, rice sheath blight, gray mold, wheat sheath blight, apple leaf spot, tomato early blight, pear black spot, bacterial wilt, bacterial leaf blight, soft rot, pepper scab, and cucumber angular leaf spot.

[0035] In one embodiment, the present invention provides the aforementioned *Ehime-like Bacillus* (… Paenibacillus from Ehime The use of strain KY1075 or the aforementioned microbial agents for the control of soybean root rot under laboratory, greenhouse and field conditions.

[0036] In another embodiment, the present invention provides the aforementioned *Ehime-like Bacillus* (… Paenibacillus from Ehime The use of strain KY1075 or the aforementioned microbial agents in the fight against plant pathogens. The plant pathogens include *Phytophthora sojae*, *Fusarium*, *Pythium*, or *Rhizoctonia solani*, and *Ralstonia solanacearum*. Ralstonia solanacearum Xanthomonas oryzae, a pathogenic strain of rice ( Xanthomonas oryzae pv. oryzae Erwinia carotene ( ), Erwinia carotovora Xanthomonas aeruginosa, a pathogenic species of pepper ( Xanthomonas campestris pv. bladder ), Pseudomonas syringae pathogenic strain in cucumber ( Pseudomonas syringae pv. lachrymans ) and the corresponding pathogens causing cotton wilt, watermelon wilt, banana wilt, wheat root rot, rice sheath blight, gray mold, wheat sheath blight, apple leaf spot, tomato early blight, and pear black spot. In addition, the aforementioned *Bacillus erythropoietinus* ( Paenibacillus ehimensis Strain KY1075 or the aforementioned microbial agents can also antagonize a dominant bacterium, Pantotheca D6, isolated from diseased plants. Pantoea agglomerans ).

[0037] In one specific embodiment, the present invention provides the aforementioned *Ehime-like Bacillus* (… Paenibacillus from Ehime The role of strain KY1075 or the aforementioned microbial agents in inhibiting or antagonizing the activity of *Phytophthora sojae*, *Fusarium*, *Pythium*, or *Rhizoctonia solani*. In a specific example, the *Fusarium* includes *Fusarium* (… Fusarium sp. The Pythium includes Phytopythium helicoides .

[0038] In another embodiment, the present invention provides a method for preventing and controlling plant diseases, the method comprising administering the aforementioned Bacillus erythropoietinus (B. erythropoietinus) Paenibacillus ehimensis The strain KY1075 or the aforementioned microbial inoculant may be applied to the plant. For example, by root irrigation and / or foliar spraying.

[0039] In summary, the aforementioned Ehime-like Bacillus ( Paenibacillus ehimensis Strain KY1075 or the aforementioned microbial agents are effective in controlling soybean root rot and other diseases, and can promote crop growth. Existing soybean biocontrol bacteria still have problems such as narrow antibacterial spectrum and low efficacy. The *Bacillus erythropoietinus* strain (KY1075) screened in this invention... Paenibacillus ehimensis The strain KY1075 is of great significance for the control of a wide variety of constantly evolving plant pathogens. The *Bacillus erythropoietinus* strain described in this invention... Paenibacillus ehimensis The strain KY1075 was used as the active ingredient to prepare a novel agricultural fungicide, which can provide good control of soybean root rot and other plant diseases, with low environmental pollution and low resistance. Furthermore, the *Bacillus erythropoietinus* strain of this invention... Paenibacillus from Ehime Compared with other Bacillus erythrophorus strains, strain KY1075 has the best overall bactericidal activity, and also has the bactericidal ability of soybean root rot pathogens and broad-spectrum antibacterial properties.

[0040] The present invention will be explained below with reference to embodiments. Those skilled in the art will understand that the following embodiments are for illustrative purposes only and should not be considered as limiting the scope of the invention. Where specific techniques or conditions are not specified in the embodiments, they are performed according to the techniques or conditions described in the literature or according to the product instructions. Reagents or instruments whose manufacturers are not specified are all commercially available conventional products. It should be noted that the conventional soybean root rot pathogens used in the embodiments of this application include *Phytophthora soysarum* and *Fusarium soysarum* (…). Fusarium sp. ), Pythium (such as Phytopythium helicoides Rhizoctonia solani and Pantotheca cumulus are common species known in the art. These strains have similar or identical properties to commercially available strains. Those skilled in the art can use other commercially available strains to perform and repeat the experimental screening operations of this application.

[0041] Example 1. Obtain microorganisms that kill soybean root rot pathogens. As mentioned above, soybean root rot is not caused by a single genus or species; its pathogens are diverse, mainly including *Phytophthora sojae*, *Fusarium sojae*, *Pythium*, and *Rhizoctonia solani*, with significant differences in their infection pathways and pathogenic mechanisms. Currently, soybean root rot is difficult to control due to a lack of targeted agents or measures. To address this industry challenge, the inventor's research team systematically collected multiple typical diseased plant samples from soybean-producing fields in Dayangshu Town, Oroqen Autonomous Banner, Hulunbuir City, Inner Mongolia Autonomous Region, and conducted a comprehensive analysis of the pathogenic microorganisms in the samples. The results showed that, in addition to isolating the fungal pathogens reported in existing literature, a dominant bacterium, *Panthera philoxeroides*, was also isolated from the diseased plants. Pantoea agglomerans (Hereinafter referred to as: pathogenic bacterium D6), the population of this bacterium in diseased tissues was significantly higher than that in healthy plants, and artificial re-inoculation experiments verified that inoculating healthy soybean plants with it could induce disease in soybean plants within 7-10 days. The *Pantotheca acuminata* strain used in this experiment (…) Pantoea agglomerans D6 and other commercially available or commercially purchased pantothecin ( Pantoea agglomerans The strains have similar or identical performance, and other commercially available or commercially purchased pantothenic bacteria can also be used for the experimental operations of this application.

[0042] Therefore, the present invention addresses the above-mentioned pathogens (Phytophthora soybata, Fusarium soybata, Pythium, Rhizoctonia solani, and pathogenic bacteria D6). Pantoea agglomerans As indicator bacteria, microorganisms that kill soybean root rot pathogens were screened and obtained. The specific operation is as follows.

[0043] 1.1 High-throughput acquisition of microorganisms capable of killing Phytophthora soybeanis Soybean Phytophthora blight (the pathogen is Phytophthora sojae) Phytophthora sojae Soybean Phytophthora blight is a typical devastating soil-borne disease. Its core characteristic is its extremely rapid onset; once it occurs in the field, it can cause widespread "sudden death" of soybeans within a short period, resulting in devastating losses to soybean production. Given the serious damage caused by soybean Phytophthora blight and the urgency of its control, this invention identifies *Phytophthora sojae* as the indicator strain for the first round of screening. The target biocontrol microorganism is obtained through a targeted screening process, the specific steps of which are as follows.

[0044] 1.1.1 Sample Collection (1) Soil sampling A total of 95 soil samples were collected from across the country, including various types of soil such as black soil, clay, and red soil, originating from forests, grasslands, wheat fields, and paddy fields. Each soil sample was labeled with its collection location (province, city, county), collection time, and collection source (forest, grassland, wheat field, paddy field, etc.).

[0045] 1.1.2 Obtaining Phytophthora in soybean The soybean Phytophthora in this invention ( Phytophthora sojae The strain of Phytophthora soybeanae used in this experiment was obtained from China Agricultural University. This strain is different from other commercially available or purchased Phytophthora soybeanae strains. Phytophthora sojae If the performance is consistent or similar, other commercially available soybean Phytophthora can also be used for the experiment.

[0046] 1.1.3 High-throughput method for obtaining microorganisms with antifungal function against Phytophthora soybeanis (1) Take 5 soil samples (0.2 g of each soil sample) as a group, mix them and place them in 50 mL of sterile water. After shaking well, take a small amount of liquid and dilute it 10 times, 100 times and 1000 times respectively. Take 100 μL of each and spread it evenly on R2A solid medium. Incubate at 30℃ for 2-4 days. (The solid R2A medium is prepared as follows: 0.5 g peptone, 0.5 g yeast extract, 0.5 g tryptone, 0.5 g glucose, 0.5 g soluble starch, 0.3 g sodium pyruvate, 0.3 g potassium dihydrogen phosphate, 0.05 g magnesium sulfate, 1000 mL H2O, 15 g agar, autoclave at 121.0℃ for 30 min).

[0047] (2) Pick the colonies from the culture dish and transfer them to a 96-well plate containing R2A solid medium and incubate at 30°C for 2 days.

[0048] (3) Scrape the mycelium of Phytophthora soybeanis and place it in sterile water. Shake well to make a bacterial suspension. Then take a small amount of bacterial suspension and spread it evenly on R2A solid medium to make selective medium A for later use.

[0049] (4) Use a sterilized microplate replicator (96 wells) to pick up the microorganisms in the 96-well microplate and copy them onto selective culture medium A. Incubate at 30°C for 2-3 days and observe the growth of microorganisms and the formation of inhibition zones.

[0050] If an inhibition zone forms around the colony, it indicates that the microorganism has antifungal activity. Specific phenomena include... Figure 1 As shown. Microorganisms that produce inhibition zones with a radius ≥1 mm were selected and purified by streaking on R2A solid medium to obtain microbial strains with the function of killing Phytophthora soybeanis.

[0051] 1.1.4 Duplicate Validation The obtained microbial strain with the function of killing Phytophthora sojae was inoculated again on selective medium A to eliminate false positive microorganisms that could not produce inhibition zones on specific mediums, and finally obtained microorganisms with the function of killing Phytophthora sojae.

[0052] 1.1.5 Results High-throughput screening of 95 soil samples (nearly 31,000 microorganisms) yielded 825 microorganisms with antifungal activity against Phytophthora soybeanis. These functional microorganisms are classified as shown in Table 1. Strains with a kill zone radius (r) of 1-2 mm formed smaller kill zones and moderate antifungal activity against Phytophthora soybeanis; these strains totaled 658. Strains with a kill zone radius greater than 2 mm formed larger kill zones and stronger antifungal activity against Phytophthora soybeanis; these strains totaled 167. To screen for target microorganisms with better control effects, this invention prioritized the 167 microorganisms with kill zone radii greater than 2 mm for further research.

[0053] Table 1. Statistical table of microorganisms verified to have the ability to kill Phytophthora soybeanis (unit: mm)

[0054] (Note: "2>r ≥ 1" indicates that the microorganism has the ability to kill Phytophthora soybeanis, but the bactericidal ability is average; "r ≥ 2" indicates that the microorganism has the ability to kill Phytophthora soybeanis and the bactericidal ability is relatively strong.)

[0055] 1.2 Obtaining microorganisms that kill soybean root rot pathogens To obtain biocontrol microorganisms that can comprehensively inhibit various pathogens causing soybean root rot, this experiment used the aforementioned pathogens (Fusarium, […]) in addition to Phytophthora soybeanum. Fusarium spp. ), Pythium ( Pythium spp. Rhizoctonia solani ( ), Rhizoctonia solani Rhizoctonia solani ) and newly isolated pathogenic bacteria D6 ( Pantoea agglomerans As an indicator bacterium, confrontation and inhibition tests were conducted, and the specific procedures are as follows.

[0056] 1.2.1 Obtaining pathogens from soybean plants with root rot 1.2.1.1 Obtaining Fusarium solani (1) Samples: Soybean plants with root rot were collected from soybean fields in Dayangshu Town, Oroqen Autonomous Banner, Hulunbuir City, Inner Mongolia Autonomous Region. Parts with typical symptoms of soybean plants were selected. (2) Sample pretreatment: Tissue at the junction of diseased and healthy tissue was cut. (3) Surface disinfection (key: kill surface bacteria and protect internal pathogens): 95% alcohol was evenly applied to the surface of the tissue with a cotton ball containing 95% alcohol and sterilized by passing it over an alcohol lamp. (4) The disinfected tissue was cut into pieces with sterile scissors, placed in a sterile mortar, and ground with sterile water. (5) Inoculation and culture: The bacterial solution was dipped into the inoculation loop and streaked on PDA medium containing chloramphenicol. The culture was carried out at 30°C for 3-5 days, and the colony growth was observed. (6) Pure Strains: When white, pink, or purple fluffy colonies (typical colony morphology of Fusarium) appear on the culture medium, use a sterile inoculation loop to pick up the hyphae from the edge of a single colony and transfer them to a new chloramphenicol-containing PDA medium. Repeat the transfer 1-2 times until a strain with uniform colony morphology and no contaminants is obtained; (7) Strains identification: 1) Morphological identification: Observe the colony color and hyphal morphology, pick up hyphae to prepare slides, and observe the large conidia (falcate, multi-septate) and small conidia (elliptical, single-septate or unseptate) unique to Fusarium under a microscope; 2) Molecular identification: Extract the DNA of the strain, amplify the ITS sequence (internal transcribed spacer region), compare it with the known sequence of Fusarium spp. to confirm the species. Other commercially available Fusarium spp. ...

[0057] 1.2.1.2 Obtaining mold from tofu (1) Samples: Soybean plants with root rot were collected from soybean fields in Dayangshu Town, Oroqen Autonomous Banner, Hulunbuir City, Inner Mongolia Autonomous Region. Parts with typical symptoms of soybean plants were selected. (2) The sterilized tissue was cut into 2-3 pieces with sterile scissors and placed evenly on PDA medium containing chloramphenicol. (3) The culture dish was inverted and placed in a constant temperature incubator at 20-23℃ (the optimal temperature for Pythium; growth is inhibited above 28℃). The culture was kept in the dark for 1-2 days, and colony growth was observed every day. (4) Purification of strains: 1) Typical colony characteristics of Pythium: Initially, it is white, transparent, cottony or fluffy, and may turn grayish-white later. The colony spreads quickly (faster than Fusarium) and has no obvious pigment (distinguishing it from the pink / purple colonies of Fusarium); 2) Use a sterile inoculation loop to pick hyphae from the edge of a single colony and transfer them to a new chloramphenicol-containing PDA medium. Incubate at 20-23℃ for 3-5 days; 3) Repeat the transfer 1-2 times until a pure strain with uniform colony morphology and no contaminating bacteria (no colonies of other colors, no slimy bacterial colonies) is obtained. (5) Strain confirmation: 1) Morphological observation: Typical colony characteristics of Pythium: Initially, it is white, transparent, cottony or fluffy, and may turn grayish-white later. The colony spreads quickly (faster than Fusarium) and has no obvious pigment (distinguishing it from the pink / purple colonies of Fusarium). This can be preliminarily confirmed. 2) Molecular identification: Extract the strain's DNA, amplify the ITS sequence or coxII gene, and compare it with the sequences of known *C. tomentosa* species to accurately determine the species. Other commercially available *C. tomentosa* species can also be used for the experiment.

[0058] 1.2.1.3 Obtaining Rhizoctonia solani The Rhizoctonia solani used in this invention ( Rhizoctonia solani The strain is from the Chinese Academy of Agricultural Sciences, but other commercially available Rhizoctonia solani strains can also be used for experiments.

[0059] 1.2.1.4 Obtaining pathogenic bacteria D6 ( Pantoea agglomerans ) (1) Samples: Soybean plants infected with root rot were collected from soybean fields in Dayangshu Town, Oroqen Autonomous Banner, Hulunbuir City, Inner Mongolia Autonomous Region. The soybean plants were selected from the parts with typical symptoms. (2) Sample pretreatment: Tissue at the junction of diseased and healthy tissue was cut. (3) Surface disinfection (key: kill surface bacteria and protect internal pathogens): 95% alcohol was evenly applied to the surface of the tissue with a cotton ball soaked in 95% alcohol and then sterilized by passing it over an alcohol lamp. (4) The disinfected tissue was cut with sterile scissors. (5) Inoculation culture: Dip the inoculation loop into the bacterial solution, streak it on the R2A medium, and incubate it at 30℃ for 2-4 days to observe the growth of the colony; (6) Purification of the strain: When the colony appears on the medium, use a sterile inoculation loop to pick a single bacterial and transfer it to a new R2A medium. Repeat the transfer 1-2 times until a strain with uniform colony morphology and no contaminants is obtained; (7) Identification of the strain: Pick a purified single colony for 16S RNA analysis; (8) Artificial inoculation test: Inoculate the pathogen onto healthy plants in a controlled manner and observe the disease to verify the pathogenicity of the pathogen. The steps are as follows: 1) Use an appropriate amount of D6 bacterial suspension (OD) 600 1) Using a sterile inoculation needle (mm=0.1), gently prick a small hole in the vein of a healthy soybean plant leaf and slowly push the bacterial suspension into the leaf; 2) Use a pipette tip to prick a small hole at the branching point of the stem of a healthy soybean plant and aspirate 30 μL of D6 bacterial suspension (OD). 600 3) The soybean plants in the control group were not treated in any special way. 4) Culture and observation: The inoculated plants were placed in a suitable environment for disease development (25℃, 12 hours of light / 12 hours of darkness, and 60%-80% humidity) and observed for 7-10 days. The disease development was recorded. This included the time of disease development (the time when the first lesion appeared) and the symptoms of disease development (the shape, color, and size of the lesion, whether it rotted, wilted, etc.). 5) Re-isolation and verification (a key step of Koch's postulates): Samples were taken from the diseased parts of the inoculated plants (such as the edge of the lesion) and the pathogen was re-isolated and cultured (the operation steps are the same as those in steps 1.2.1.4 (1)-(6) above). The pure culture of bacteria consistent with the initial inoculation was observed. If the same pathogen was re-isolated, combined with the symptoms of disease development, the pathogen could be confirmed as the pathogenic pathogen (satisfying Koch's postulates). Other commercially available clumps of pantothecin can also be used for the experiment.

[0060] 1.2.2 Obtaining biocontrol microorganisms that kill the pathogenic fungus causing soybean root rot Fusarium solani, Pythium spp., and Rhizoctonia solani were used as indicator strains. The 167 microorganisms verified to have fungicidal activity against Phytophthora solani in step "1.1.4" were used as test strains. Indicator strain mycelium discs were inoculated in the center of R2A solid medium, and each test strain was inoculated in four directions at a distance of 3 cm from the mycelium disc. The medium was incubated at 30°C for 8 days. After incubation, the growth of the microorganisms and the formation of inhibition zones were observed.

[0061] If an inhibition zone forms around the colony, it indicates that the microorganism has antifungal activity. Specific phenomena include... Figure 2 As shown. Microorganisms that produce inhibition zones with a radius ≥1 mm were selected and purified by streaking on R2A solid medium to obtain microbial strains with the function of killing Phytophthora soybeanis.

[0062] 1.2.3 Obtaining pathogenic bacteria isolated from soybean root rot sites. The pathogenic bacteria isolated from the root rot of soybeans were set as indicator strains. The 167 microorganisms that had been verified to have fungicidal activity against Phytophthora beannulata in the aforementioned step “1.1.4” were used as test strains for antibacterial tests. The specific steps are as follows: (1) Activate the pathogenic bacteria D6 isolated from the root rot of soybeans; (2) Prepare a bacterial suspension of strain D6 obtained in the previous step, inoculate it into R2A solid medium and make its OD≈0.01 to obtain selective medium B; (3) Spot the microorganisms that have been verified to have fungicidal activity against Phytophthora beannulata on selective medium B and culture them at 30°C for 2 days.

[0063] Observe the growth of microorganisms and the formation of inhibition zones. If inhibition zones are formed around the colonies, it indicates that the microorganisms have bactericidal activity. Select microorganisms that produce inhibition zones with a radius ≥1 mm, streak them on R2A solid medium for purification, and obtain microbial strains with the function of killing pathogenic bacteria D6.

[0064] 1.2.4 Results 1.2.4.1 Identification results of pathogens obtained from soybean plants with root rot Two fungal strains and one pathogenic bacterium were isolated from soybean plants with rotten roots collected from soybean fields in Dayangshu Town, Oroqen Autonomous Banner, Hulunbuir City, Inner Mongolia Autonomous Region. The morphology of the fungal strains was observed (results are shown in Table 2), and DNA was extracted from several fungal strains. The ITS sequence or coxII gene was amplified and compared with known sequences, confirming them as Fusarium (Fusarium tumefaciens). Fusarium sp. )and Phytopythium helicoides Fusarium can infect soybeans and cause soybean root rot. Phytopythium helical (Phytophthora), originally classified as Pythium ( Pythium However, later taxonomic revisions classified it as a separate genus, Phytophthora. Phytopythium*D. spp.* is a plant pathogenic oomycete that primarily infects crop roots, causing root rot and damping-off. It infects soybeans, mainly causing root rot, and often co-infects with other soil-borne pathogens (such as *Fusarium*), exacerbating the damage. Based on the obtained 16S rDNA sequence of the pathogenic bacterium D6, homologous sequences were searched in GenBank and compared using homologous sequence analysis. Simultaneously, sequence alignment was performed with 16S RNA databases recognized by the International Committee for Bacteriology, combined with literature analysis, to determine the taxonomic position of the target microorganism (Yoon, SH, Ha, SM, Kwon, S., Lim, J., Kim, Y., Seo, H. and Chun, J. (2017). Introducing EzBioCloud: A taxonomically united database of 16S rRNA and whole genome assemblies. Int J Syst Evol Microbiol. 67:1613-1617). The results showed that the base sequence of this strain was similar to that of strain D6. Pantoea agglomerans With high homology, strain D6 was confirmed to be... Pantoea agglomerans Artificial inoculation experiments have verified that inoculating healthy soybean plants with this method can induce disease in soybean plants within 7-10 days (results are shown in the figure). Figure 3 (As shown).

[0065] Table 2. Morphological description of fungi obtained from soybean root rot plants

[0066] 1.2.4.2 Obtain the results of biocontrol microbial experiments that can comprehensively inhibit various pathogens causing soybean root rot. Confrontation and inhibition tests were conducted on 167 microorganisms that had been verified to have fungicidal activity against Phytophthora soybeanis (targeting Phytophthora soybeanis, Fusarium solani, Pythium spp., Rhizoctonia solani, and newly isolated pathogenic bacteria). The results are shown in Table 3.

[0067] Table 3. Statistical table of microorganisms verified to have the ability to kill soybean root rot pathogens.

[0068] Of the 167 microorganisms verified to have fungicidal activity against *Phytophthora sojae*, 66 were effective against *Fusarium sojae*, 32 against *Phytophthora macrantha*, 45 against *Rhizoctonia solani*, and 23 against pathogenic bacteria D6. Among these, 10 microorganisms showed fungicidal activity against all five pathogens, effectively inhibiting various pathogens causing soybean root rot. These 10 microorganisms meet the criteria for being the target microorganisms of this invention (microorganisms for controlling soybean root rot). Therefore, these 10 microorganisms, verified to be effective against soybean root rot pathogens, were selected for further verification.

[0069] 2. Screening for broad-spectrum biocontrol microorganisms that can control pathogens of important legume diseases and other important crop diseases. Besides soybeans, peanuts and green beans are also important legume crops. Peanut white mold, a common soil-borne disease, poses a significant threat to the safe production of these legumes. In addition, cotton wilt, banana wilt, watermelon wilt, gray mold, wheat sheath blight, and bacterial wilt are also important plant diseases causing serious losses in agricultural production. To broaden the application scenarios of this invention (covering other legume crops and other major agricultural crops), this study used microorganisms with proven fungicidal activity against soybean root rot pathogens as the starting strain. Through confrontation experiments against various pathogenic fungi and antibacterial experiments against pathogenic bacteria, broad-spectrum biocontrol microorganisms were screened and obtained. The specific steps are as follows.

[0070] 2.1 Obtaining microorganisms that can control the pathogen of peanut white mold. The pathogen of peanut white mold was used as the indicator strain. Ten microorganisms verified to have fungicidal activity against the pathogen of soybean root rot, as described in step "1.2.4," were used as test strains for a confrontation experiment, with specific steps following "1.2.2." The experiment was conducted at 30℃ for 3 days. After incubation, the strength of the antagonistic effect of the strains against the peanut white mold pathogen was observed. The width of the fungicidal zone (the width of the transparent inhibition zone between the test strain colony and the indicator strain colony; 0 mm was recorded when there was no inhibition zone) and the colony radius of the test strain were measured and recorded using the cross-crossing method. The fungicidal intensity (E1) was then calculated based on these indicators to evaluate the antagonistic ability of the test strains against the indicator pathogen (a higher fungicidal intensity value indicates a stronger fungicidal ability against the corresponding pathogen; a lower value indicates a weaker fungicidal ability).

[0071]

[0072] 2.2 Obtaining broad-spectrum biocontrol microorganisms capable of controlling major plant disease pathogens. The pathogens of 12 plant diseases were used as indicator strains, including three specialized types of cotton wilt, watermelon wilt, banana wilt, wheat root rot, rice sheath blight, wheat sheath blight, gray mold, tomato early blight, pear black spot, and apple leaf spot. Ten microorganisms from section "1.2.4" that had been verified to have fungicidal activity against the soybean root rot pathogen were used as test strains for a broad-spectrum test against major crop disease pathogens (confrontation experiment). The specific steps are as described in "1.2.2". The experiment was conducted at 30℃ for 8 days. After cultivation, the strength of the antagonistic effect of the strains against plant pathogenic fungi was observed. The width of the fungicidal zone (the width of the transparent inhibition zone between the colonies of the test strain and the indicator strain, recorded as 0 mm when there is no inhibition zone) and the radius of the test strain colony were measured and recorded using the cross-cross method. The fungicidal intensity (E1) was then calculated based on the above indicators to evaluate the strength of the antagonistic ability of the test strains against each indicator pathogenic fungus (the larger the fungicidal intensity value, the stronger the fungicidal ability of the microorganism against the corresponding pathogenic fungus; the smaller the fungicidal intensity value, the weaker the fungicidal ability of the microorganism against the corresponding pathogenic fungus).

[0073]

[0074] 2.3 Obtaining broad-spectrum biocontrol microorganisms capable of controlling major plant disease pathogens. The pathogens of five bacterial diseases of crops, namely bacterial wilt, bacterial leaf blight, soft rot, pepper scab, and cucumber angular leaf spot, were set as indicator strains. Ten microorganisms in "1.2.4" that have been verified to have fungicidal activity against the pathogen of soybean root rot were used as test strains to conduct a broad-spectrum test for the pathogens of bacterial diseases (antibacterial test). The specific steps are as follows: (1) Activate the pathogens of bacterial wilt, bacterial leaf blight, soft rot, pepper scab, and cucumber angular leaf spot (the pathogens are Ralstonia solanacearum in order of their order of pathogens). Ralstonia solanacearum Xanthomonas oryzae, a pathogenic strain of rice ( Xanthomonas oryzae pv. rice Erwinia carotene ( ), Erwinia carotovora Xanthomonas aeruginosa, a pathogenic species of pepper ( Xanthomonas campestris pv. bladder ), Pseudomonas syringae pathogenic strain in cucumber ( Pseudomonas syringae pv. lachrymans(2) Preparation of indicator bacteria plates: The five activated pathogens were prepared into bacterial suspensions, and then each bacterial suspension was evenly distributed in R2A solid medium and the concentration of pathogens in the final medium was OD≈0.01. Selective mediums containing different indicator bacteria were prepared and labeled as medium B (containing bacterial wilt), C (containing bacterial white wilt), D (containing bacterial soft rot), E (containing pepper scab), and F (containing cucumber angular leaf spot); (3) Inoculation and culture of test strains: The microorganisms that have been verified to have bactericidal activity against soybean root rot pathogens were placed on selective mediums B, C, D, E, and F and cultured at 30°C for 2 days; (4) Index determination and antagonistic ability evaluation: After the culture was completed, the strength of the antagonism of the strains against each pathogen was observed. The width of the bactericidal zone (the width of the transparent inhibition zone between the colony of the test strain and the colony of the indicator strain, and 0 when there is no inhibition zone) was measured and recorded using the cross-cross method. (5) Calculate the bactericidal intensity (E2) and evaluate the bactericidal ability of the test strain against each indicator pathogen by the value of the bactericidal intensity: the larger the value of the bactericidal intensity, the stronger the bactericidal ability of the microorganism against the corresponding pathogen; the smaller the value of the bactericidal intensity, the weaker the bactericidal ability of the microorganism against the corresponding pathogen.

[0075] , (X represents the transparency of the sterilization zone. If the sterilization zone is completely transparent, X is 2; if the sterilization zone is semi-transparent, X is 1).

[0076] 2.4 Results Using microorganisms that have been verified to have fungicidal activity against soybean root rot pathogens as starting strains, three types of antibacterial experiments were conducted: first, a confrontation experiment against peanut white mold pathogen; second, a confrontation experiment against 12 fungal plant disease pathogens such as cotton wilt; and third, an antibacterial experiment against 5 bacterial plant disease pathogens such as bacterial wilt and bacterial leaf blight. The results are shown in Table 4.

[0077] Table 4. Comparison of the fungicidal activity of 10 microorganisms with proven ability to kill soybean root rot pathogens against 18 plant pathogens. .

[0078] (Note: The higher the bactericidal strength value, the stronger the bactericidal ability of the microorganism against the corresponding pathogen; the lower the bactericidal strength value, the weaker the bactericidal ability of the microorganism against the corresponding pathogen; "0" indicates that the microorganism has no bactericidal ability against the corresponding pathogen.)

[0079] 16S rDNA sequence analysis identified 10 test microorganisms into 7 species, namely... Paenibacillus from EhimeOil-degrading Bacillus ( Paenibacillus oleatilyticus ), Demiella spp. ( Paenibacillus jamilae Bacillus belesiensis ( Bacillus paramobilis ), Polymyxin Bacillus ( Paenibacillus polymyxa ), Bacillus velezensis , Paenibacillus polymyxa .in, Paenibacillus ehimensis The highest proportion was found in four strains: KY1075, J1020, 45-3b8-5, and 230-9③. Therefore, this strain was the focus of our analysis.

[0080] The results showed that 4 plants Paenibacillus ehimensis Significant differences were observed in the fungicidal activity and spectrum against 18 plant pathogens. Specifically, 45-3b8-5 and 230-9③ showed fungicidal activity against only some pathogens (fungicide activity > 0); KY1075 and J1020 showed fungicidal activity against all 18 pathogens (fungicide activity > 0), with KY1075 exhibiting superior overall fungicidal activity compared to J1020. In conclusion, KY1075 is a biocontrol microorganism with high development potential, possessing both fungicidal activity against soybean root rot pathogens and broad-spectrum antifungal properties. Therefore, this invention uses it as the research subject for further research.

[0081] 3. Obtain broad-spectrum biocontrol microorganisms that exhibit bactericidal activity under various culture conditions. Soil environments are complex, placing extremely high demands on the adaptability of microorganisms to culture conditions. If a microbial strain can inhibit plant pathogens under different survival conditions, its success rate and stability in controlling related diseases in the field will be superior. Therefore, this invention further designs a confrontation experiment under multiple culture medium conditions for KY1075, a broad-spectrum biocontrol microorganism with verified bactericidal activity against soybean root rot pathogens, obtained in section "2.4". By simulating different potential field environmental conditions, the invention verifies and screens out broad-spectrum biocontrol microorganisms that maintain stable bactericidal activity under various culture conditions. The specific operation is as follows.

[0082] 3.1 Obtaining broad-spectrum biocontrol microorganisms with bactericidal activity under R2A medium conditions Seventeen pathogens were selected as indicator strains, including those causing soybean root rot (Phytophthora soybean, Fusarium, Pythium, Rhizoctonia solani), peanut white mold, cotton wilt, watermelon wilt, banana wilt (three specialized types), root rot, wheat root rot, rice sheath blight, gray mold, tomato early blight, pear black spot, and apple leaf spot. KY1075, a broad-spectrum biocontrol microorganism verified in section 2.4 to have fungicidal activity against the soybean root rot pathogen, was selected as the test strain. The test strain and each indicator strain were simultaneously inoculated onto R2A solid medium and cultured at 30°C (3 days for peanut white mold, 2-3 days for Pythium, and 8 days for the others). After cultivation, the antagonistic effect of strain KY1075 on pathogenic fungi was observed. The width of the bactericidal zone (the width of the transparent inhibition zone between the colony of the test strain and the colony of the indicator strain, with 0 mm recorded when there is no inhibition zone) and the colony radius of the test strain were measured and recorded using the cross-cross method. The fungicidal intensity (E1) was then calculated based on the above indicators to evaluate the antagonistic ability of the test strain against each indicator pathogenic fungus (the larger the fungicidal intensity value, the stronger the bactericidal ability of the microorganism against the corresponding pathogenic fungus; the smaller the fungicidal intensity value, the weaker the bactericidal ability of the microorganism against the corresponding pathogenic fungus). (The solid R2A medium is prepared as follows: 0.5 g peptone, 0.5 g yeast extract, 0.5 g tryptone, 0.5 g glucose, 0.5 g soluble starch, 0.3 g sodium pyruvate, 0.3 g potassium dihydrogen phosphate, 0.05 g magnesium sulfate, 1000 mL H2O, 15 g agar, autoclave at 121.0℃ for 30 min).

[0083] 3.2 Obtaining broad-spectrum biocontrol microorganisms with bactericidal activity under PDA culture medium conditions Seventeen pathogens of soybean root rot (Phytophthora soybean, Fusarium, Pythium, Rhizoctonia solani), peanut white mold, cotton wilt, watermelon wilt, banana wilt (three specialized types), root rot, wheat root rot, rice sheath blight, gray mold, tomato early blight, pear black spot, and apple leaf spot were selected as indicator strains. KY1075, a broad-spectrum biocontrol microorganism verified in section 2.4 to have fungicidal activity against the soybean root rot pathogen, was selected as the test strain. The test strain and each indicator strain were simultaneously inoculated onto PDA solid medium and cultured at 30°C (3 days for peanut white mold, 2-3 days for Pythium, and 8 days for the others). After cultivation, the antagonistic effect of strain KY1075 against pathogenic fungi was observed. The width of the inhibition zone (the width of the transparent inhibition area between the colonies of the test strain and the indicator strain, recorded as 0 mm when there is no inhibition zone) and the radius of the test strain colony were measured and recorded using the cross-cross method. The fungicidal intensity (E1) was then calculated based on the above indicators to evaluate the antagonistic ability of the test strain against each indicator pathogenic fungus (the higher the fungicidal intensity value, the stronger the fungicidal ability of the microorganism against the corresponding pathogenic fungus; the lower the fungicidal intensity value, the weaker the fungicidal ability of the microorganism against the corresponding pathogenic fungus). (The solid PDA medium used in this test was purchased from Guangdong Huankai Biotechnology Co., Ltd., batch number: 240319A20).

[0084] 3.3 Obtaining broad-spectrum biocontrol microorganisms with bactericidal activity under NA culture medium conditions Seventeen pathogens of soybean root rot (Phytophthora soybean, Fusarium, Pythium, Rhizoctonia solani), peanut white mold, cotton wilt, watermelon wilt, banana wilt (three specialized types), root rot, wheat root rot, rice sheath blight, gray mold, tomato early blight, pear black spot, and apple leaf spot were selected as indicator strains. KY1075, a broad-spectrum biocontrol microorganism verified in section 2.4 to have fungicidal activity against the soybean root rot pathogen, was selected as the test strain. The test strain and each indicator strain were simultaneously inoculated onto NA solid medium and cultured at 30°C (3 days for peanut white mold, 2-3 days for Pythium, and 8 days for the others). After cultivation, the antagonistic effect of strain KY1075 against pathogenic fungi was observed. The width of the inhibition zone (the width of the transparent inhibition area between the test strain colony and the indicator strain colony; 0 mm was recorded when there was no inhibition zone) and the colony radius of the test strain were measured and recorded using the cross-cross method. The fungicidal intensity (E1) was then calculated based on these indicators to evaluate the antagonistic ability of the test strain against each indicator pathogenic fungus. (A higher fungicidal intensity value indicates a stronger fungicidal ability against the corresponding pathogenic fungus; a lower fungicidal intensity value indicates a weaker fungicidal ability against the corresponding pathogenic fungus. Solid NA medium was prepared as follows: 10.0 g peptone, 5.0 g sodium chloride, 3.0 g beef meal, 1000 mL H2O, 15 g agar, autoclaved at 121.0℃ for 30 min.)

[0085] 3.4 Obtaining broad-spectrum biocontrol microorganisms with bactericidal activity under LB medium conditions Seventeen pathogens of soybean root rot (Phytophthora soybean, Fusarium, Pythium, Rhizoctonia solani), peanut white mold, cotton wilt, watermelon wilt, banana wilt (three specialized types), root rot, wheat root rot, rice sheath blight, gray mold, tomato early blight, pear black spot, and apple leaf spot were selected as indicator strains. KY1075, a broad-spectrum biocontrol microorganism verified in section 2.4 to have fungicidal activity against the soybean root rot pathogen, was selected as the test strain. The test strain and each indicator strain were simultaneously inoculated onto LB solid medium and cultured at 30°C (3 days for peanut white mold, 2-3 days for Pythium, and 8 days for the others). After cultivation, the antagonistic effect of strain KY1075 on pathogenic fungi was observed. The width of the bactericidal zone (the width of the transparent inhibition zone between the colony of the test strain and the colony of the indicator strain, with 0 mm recorded when there is no inhibition zone) and the colony radius of the test strain were measured and recorded using the cross-cross method. The fungicidal intensity (E1) was then calculated based on the above indicators to evaluate the antagonistic ability of the test strain against each indicator pathogenic fungus. (The larger the fungicidal intensity value, the stronger the bactericidal ability of the microorganism against the corresponding pathogenic fungus; the smaller the fungicidal intensity value, the weaker the bactericidal ability of the microorganism against the corresponding pathogenic fungus. The solid LB medium was prepared as follows: 10.0 g peptone, 5.0 g yeast extract, 10.0 g sodium chloride, 1000 mL H2O, 15 g agar, autoclaved at 121.0℃ for 30 min.)

[0086] 3.5 Results Under various culture medium conditions, confrontation experiments were conducted on KY1075, a broad-spectrum biocontrol microorganism with proven fungicidal activity against soybean root rot pathogen, against 17 major plant disease pathogens. The results showed that KY1075 exhibited stable and excellent fungicidal activity against all 17 plant disease pathogens under four different culture medium conditions. It is a broad-spectrum biocontrol microorganism capable of killing soybean root rot pathogens under multiple culture medium conditions, meeting the expected target microorganism of this invention. KY1075 has significant biocontrol potential and market application prospects; therefore, it has been designated as the patented strain of this invention for further research.

[0087] Table 5. Fungicidal activity of KY1075 against 17 plant pathogens under four different culture medium conditions.

[0088] (Note: The higher the bactericidal strength value, the stronger the bactericidal ability of the microorganism against the corresponding pathogen; the lower the bactericidal strength value, the weaker the bactericidal ability of the microorganism against the corresponding pathogen; "0" indicates that the microorganism has no bactericidal ability against the corresponding pathogen.)

[0089] 4. Identification of strain KY1075 4.1 Preparation of DNA Template Pick a purified single colony and place it at the bottom of an EP tube. Add 200 μL of 5% (w / v) BT-chelex 100 (prepared with distilled water and sterilized at 121℃ for 30 min). Boil in a water bath for 15 min, then quickly freeze at -20℃ or -80℃. Thaw at room temperature, centrifuge at 6000 rpm for 3 min, and use 2 μL of the supernatant as a template. Amplify the 16S gene according to the 16S amplification system.

[0090] 16S PCR amplification system: 25 μL Green taqMix: 12.5 μL DDH2O: 9.5 μL AE: 0.5 μL 1492 R: 0.5 μL DNA template: 2.0 uL Total: 25 μL (The sequence of AE is: 5'-CAGAAAGCCGCCTTCGCCAC-3', SEQ ID NO: 2; the sequence of 1492R is: 5'-GGTTACCTTGTTACGACTT-3', SEQ ID NO: 3).

[0091] PCR amplification program: Step 1: 95℃, 5 min; Step 2: 94℃, 1 min; 55℃, 1 min; 72℃, 1.5 min, 35 cycles; Step 3: 72℃, 10 min; 4℃, hold.

[0092] 4.2 Results of 16S rDNA sequencing of KY1075 The 16S rDNA sequence of KY1075 is as follows:

[0093] Based on the obtained 16S rDNA sequence of KY1075, homologous sequences were searched in GenBank and compared with each other. Simultaneously, sequence alignment was performed with 16S RNA databases recognized by the International Committee for Bacteriology, and combined with literature analysis to determine the taxonomic position of the target microorganism (Yoon, SH, Ha, SM, Kwon, S., Lim, J., Kim, Y., Seo, H. and Chun, J. (2017). Introducing EzBioCloud: A taxonomically united database of 16S rRNA and whole genome assemblies. Int J Syst Evol Microbiol.67:1613-1617). The results showed that the 1390-base sequence of this strain was consistent with that of strain KY1075. Paenibacillus ehimensis It exhibits high homology, with a similarity of up to 99.57%. It has been deposited at the China General Microbiological Culture Collection Center, with accession number CGMCC No. 35207. 5. Morphological observation of strain KY1075 5.1 Procedure: Inoculate the selected strains onto R2A plates and incubate at 30°C for 2 days. Observe the size, shape, color, gloss, viscosity, raised shape, transparency, edge characteristics, and presence or absence of spores of the colonies.

[0094] 5.2 Results of strain morphology observation: After observing strain KY1075 ( Paenibacillus ehimensis After culturing on R2A medium for 2 days, the colonies were milky white to pale yellow, translucent, with smooth edges and a waxy appearance. Microscopic examination (100x oil immersion) revealed a bacterial length of approximately 1.53–2.00 μm. Figure 4 ).

[0095] 6. Comparison of KY1075 with Bacillus polymyxa and Bacillus belyssus Polymyxin Bacillus ( Paenibacillus polymyxa ) and Bacillus belesi ( Bacillus from VelezThese are representative strains widely reported in the current biocontrol field and with excellent market application feedback. Due to their broad-spectrum antibacterial activity and environmental adaptability, both *Bacillus polymyxa* and *Bacillus belyssiensis* are often used as reference strains for verifying biocontrol functions. To further clarify the biocontrol potential and application value of strain KY1075, and in accordance with the research objectives of this study, plate confrontation and inhibition tests were conducted. Using *Bacillus polymyxa* and *Bacillus belyssiensis* as controls, the inhibition zone size and other indicators of KY1075 against target pathogens (the pathogen of soybean root rot and important plant disease pathogenic fungi) were systematically compared to calculate the fungicidal intensity, thereby quantitatively evaluating its fungicidal ability and biocontrol advantages. The specific procedures are as follows.

[0096] 6.1 Comparison of the antifungal activity of KY1075 with Bacillus polymyxa and Bacillus belye against plant pathogens Four pathogens of soybean root rot (Phytophthora soyba, Fusarium wilt, Pythium spp., and Rhizoctonia solani) and seven plant disease pathogens of peanut white mold, cotton wilt, and watermelon wilt were set as indicator strains. Bacillus polymyxa, Bacillus belyssus, and KY1075 were set as test strains. Confrontational experiments were conducted on R2A, PDA, NA, and LB solid media to test fungicidal activity. The test strains and each indicator strain were simultaneously inoculated on the four solid media and cultured at 30°C (3 days for peanut white mold, 2-3 days for Pythium spp., and 8 days for the others). After cultivation, the strength of the antagonistic effect of the strains against pathogenic fungi was observed. The width of the bactericidal zone (the width of the transparent inhibition zone between the colonies of the test strain and the indicator strain, recorded as 0 mm when there is no inhibition zone) and the radius of the test strain colony were measured and recorded using the cross-cross method. The fungicidal intensity (E1) was then calculated based on the above indicators to evaluate the strength of the antagonistic ability of the test strains against each indicator pathogenic fungus (the larger the fungicidal intensity value, the stronger the bactericidal ability of the microorganism against the corresponding pathogenic fungus; the smaller the fungicidal intensity value, the weaker the bactericidal ability of the microorganism against the corresponding pathogenic fungus).

[0097] .

[0098] 6.2 Comparison of the antibacterial activity of KY1075 with Bacillus polymyxa and Bacillus belye against plant pathogens Set up bacterial wilt disease (the pathogen is Ralstonia solanacearum) Ralstonia solanacearum Bacterial leaf blight () Xanthomonas oryzae pv. oryzae ), soft rot (Erwinia carotene) Erwinia carrot-eating ), pepper scab disease ( Xanthomonas campestris pv. bladder ), cucumber angular leaf spot ( Pseudomonas syringae pv. lachrymans ), pathogenic bacteria D6 ( Pantoea agglomeransSix bacterial pathogens of crops were used as indicator strains, and *Bacillus polymyxa*, *Bacillus bereaves*, and KY1075 were used as test strains for broad-spectrum antibacterial testing (antimicrobial inhibition test). Specific steps are detailed in "2.3". The strains were incubated at 30℃ for 3 days. After incubation, the strength of the antagonistic effect of the strains on the pathogens was observed. The width of the inhibition zone (the width of the transparent inhibition area between the test strain colony and the indicator strain colony; 0 mm was recorded if no inhibition zone was observed) and the colony radius of the test strain were measured and recorded using the cross-hatching method. The fungicidal intensity (E2) was then calculated based on these indicators. The fungicidal intensity value was used to evaluate the fungicidal ability of the test strains against each indicator pathogen: a higher fungicidal intensity value indicates a stronger fungicidal ability against the corresponding pathogen; a lower fungicidal intensity value indicates a weaker fungicidal ability against the corresponding pathogen.

[0099] , (X represents the transparency of the sterilization zone. If the sterilization zone is completely transparent, X is 2; if the sterilization zone is semi-transparent, X is 1).

[0100] 6.3 Results 6.3.1 Comparison of the antifungal activity of KY1075, Bacillus polymyxa, and Bacillus belye against plant pathogens. Plate confrontation experiments were conducted under four different culture medium conditions, using *Bacillus polymyxa* and *Bacillus belyss* as controls, to systematically compare the fungicidal activity of KY1075 against 15 plant pathogenic fungi and evaluate its fungicidal ability. The results are shown in Table 6. Figure 5 , Figure 6 As shown in the figure, under four different culture conditions, KY1075 exhibited fungicidal activity against all seven plant pathogenic fungi with a strength greater than 0. However, *Bacillus polymyxa* and *Bacillus belyi* only showed fungicidal activity against a few specific plant pathogenic fungi under certain culture conditions. For example, *Bacillus polymyxa* showed greater than 0 fungicidal activity against *Phytophthora sojae* in LB, NA, and R2A media, but no fungicidal activity against *Phytophthora sojae* in PDA media; similarly, *Bacillus belyi* showed no fungicidal activity against *Fusarium wilt* pathogens in LB, PDA, and R2A media, but no fungicidal activity against *Fusarium wilt* pathogens in NA media. In addition, besides the fact that Bacillus polymyxa showed greater bactericidal activity against Rhizoctonia solani in PDA medium than KY1075, KY1075 also showed greater bactericidal activity against seven plant pathogenic fungi under four different culture medium conditions than Bacillus polymyxa and Bacillus belyss. In the current system, strain KY1075 exhibits stronger activity against plant pathogenic fungi than Bacillus polymyxa and Bacillus belyss.

[0101] 6.3.2 Comparison of the antibacterial activity of KY1075 with Bacillus polymyxa and Bacillus belye against plant pathogens Through antibacterial tests, using Bacillus polymyxa and Bacillus belyss as controls, the fungicidal activity of KY1075 against six plant pathogenic fungi was systematically compared, and its fungicidal ability was evaluated. The results are shown in Table 7. Figure 7 As shown, KY1075 exhibited bactericidal activity against all six plant pathogenic bacteria with a strength greater than 0, while *Bacillus polymyxa* and *Bacillus belyceta* showed bactericidal activity only against a few plant pathogenic fungi. Furthermore, under four different culture medium conditions, KY1075 demonstrated greater bactericidal activity against all six plant pathogenic bacteria than *Bacillus polymyxa* and *Bacillus belyceta*. Therefore, in the current system, strain KY1075 showed stronger bactericidal activity against plant pathogenic bacteria than *Bacillus polymyxa* and *Bacillus belyceta*.

[0102] In summary, in the fungicide tests against various plant pathogenic fungi and bacteria, strain KY1075 demonstrated fungicide activity against Bacillus polymyxa and Bacillus belye, showing great market potential and application prospects.

[0103] Table 6. Comparison of the fungicidal activity of three strains (KY1075, Bacillus polymyxa, and Bacillus belye) against seven plant pathogenic fungi on four different culture media.

[0104] Table 7. Comparison of bactericidal activity of three strains (KY1075, Bacillus polymyxa, and Bacillus belyssus) against six plant pathogenic bacteria on four different culture media.

[0105] This invention utilizes high-throughput screening to isolate the multifunctional biocontrol microbial strain KY1075 from soil samples from multiple locations across China. 16S rDNA sequencing analysis revealed that this strain is related to… Paenibacillus ehimensis They exhibit high homology; further analysis revealed that they belong to the same genus. Paenibacillus ehimensisThe four strains showed significant differences in fungicidal spectrum and activity, with KY1075 exhibiting the broadest spectrum and strongest activity. Activity tests were conducted in four media: R2A, PDA, LB, and NA. The results showed that KY1075 not only demonstrated significant specific fungicidal activity against all pathogens causing soybean root rot, but also exhibited excellent broad-spectrum inhibitory effects against 17 plant pathogenic fungi and 6 plant pathogenic bacteria. Comparison with commercial strains *Bacillus polymyxa* and *Bacillus belye* revealed that KY1075 showed superior inhibitory activity against multiple plant pathogenic fungi and bacteria in all four media. In conclusion, KY1075 is a multifunctional biocontrol microorganism possessing both broad-spectrum resistance to plant pathogenic fungi / bacteria and specific control against all pathogens causing soybean root rot, showing great application potential in the development of the soybean industry and the control of soil-borne diseases.

[0106] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

Claims

1. A strain of *Bacillus erythropoietinus* ( Paenibacillus ehimensis strain KY1075, characterized in that, The strain KY1075 is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 35207, and the deposit date is July 11, 2025.

2. The *Ehime*-like bacillus according to claim 1 ( Paenibacillus ehimensis The use of strain KY1075 in the control of plant diseases, wherein the plant diseases are selected from one or more of the following: Soybean root rot, peanut white mold, cotton wilt, watermelon wilt, banana wilt, wheat root rot, rice sheath blight, gray mold, wheat sheath blight, apple leaf spot, tomato early blight, pear black spot, bacterial wilt, bacterial leaf blight, soft rot, pepper scab, and cucumber angular leaf spot.

3. The use according to claim 2, characterized in that, The soybean root rot is caused by Phytophthora soybean (… Phytophthora sojae Fusarium ( ) Fusarium spp. ), Pythium ( Pythium spp. ) and Rhizoctonia solani ( Rhizoctonia solani It is caused by a single infection or multiple combined infections in one of the following.

4. The *Ehime*-like bacillus according to claim 1 ( Paenibacillus ehimensis The use of strain KY1075 in resistance to plant pathogens, wherein the plant pathogen is selected from one or more of the following: Phytophthora soybeani (… Phytophthora sojae Fusarium ( ) Fusarium spp. ), Pythium ( Pythium spp. Rhizoctonia solani ( ), Rhizoctonia solani Rhizoctonia solani Ralstonia solanacearum (Ralstonia solanacearum) Ralstonia solanacearum Xanthomonas oryzae, a pathogenic strain of rice ( Xanthomonas oryzae pv. oryzae Erwinia carotene ( ), Erwinia carotovora Xanthomonas aeruginosa, a pathogenic species of pepper ( Xanthomonas campestris pv. vesicatoria ), Pseudomonas syringae pathogenic strain in cucumber ( Pseudomonas syringae pv. lachrymans ) and clusters of pantothenic bacteria ( Pantoea agglomerans ).

5. A method for preventing and controlling plant diseases, characterized in that, The method includes dispensing the *Bacillus erythropoietin* as described in claim 1 (… Paenibacillus ehimensis The strain KY1075 was applied to the plant.

6. The method according to claim 5, characterized in that, The method includes dispensing the *Bacillus erythropoietin* as described in claim 1 (… Paenibacillus ehimensis The strain KY1075 may be applied to the seeds or leaves of the plant, or to the roots of the plant.

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