通过疮痂病链霉菌SCAB_82261基因提高thaxtomin A产量的方法
By deleting the SCAB_82261 gene in *Streptomyces scabica*, phenylalanine degradation was blocked and the transcription of related genes was upregulated, thus solving the problem of insufficient thaxtomin A production in existing technologies and achieving highly efficient biosynthesis.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- ANHUI AGRICULTURAL UNIVERSITY
- Filing Date
- 2026-01-28
- Publication Date
- 2026-07-17
AI Technical Summary
In the existing technology, there is limited research on the function of PaaK protein in the production of microbial drugs, which limits the development of technology to optimize thaxtomin A synthesis through targeted metabolic regulation. How to increase the yield of thaxtomin A in Streptomyces scabies has become a research hotspot.
By deleting the SCAB_82261 gene in *Streptomyces scabica* through genetic engineering, the degradation and metabolism of phenylalanine were blocked, increasing the accumulation of intracellular phenylalanine and upregulating the transcriptional levels of txtR, txtA, and txtE genes in the thaxtomin A biosynthetic gene cluster, thereby increasing the biosynthetic yield of thaxtomin A.
It significantly increased the yield of thaxtomin A to 110.5%, and the gene complementation verified that the SCAB_82261 gene is a negative regulator, providing technical support for the industrial production of high-yielding thaxtomin A strains.
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Figure CN121915067B_ABST