与小麦籽粒镉累积性状相关的KASP分子标记、引物与应用
By developing KASP molecular markers and primers at position 2766414 of chromosome 6A in the wheat genome, the problems of low efficiency in screening for cadmium accumulation traits in wheat grains in existing technologies have been solved, enabling rapid and accurate screening for low cadmium accumulation traits in grains and improving breeding efficiency.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- SHENZHEN RESEARCH INSTITUTE OF NORTHWEST A & F UNIVERSITY
- Filing Date
- 2026-03-24
- Publication Date
- 2026-07-17
AI Technical Summary
Existing molecular marker technologies for screening and breeding wheat grain cadmium accumulation traits suffer from problems such as complex operation, high cost, low throughput, poor stability and reproducibility, making it difficult to meet the needs of efficient and large-scale typing.
A competitive allele-specific PCR (KASP) molecular marker was developed, located at position 2766414 on chromosome 6A of the wheat genome. PCR amplification was performed using a specific set of KASP primers, and fluorescent labeling was used to distinguish between high and low cadmium accumulation traits in grains, achieving rapid and accurate genotyping.
This technology enables rapid screening of wheat seedlings for low cadmium accumulation, improving breeding efficiency and shortening the breeding cycle. It has broad application prospects and can accurately distinguish wheat varieties with high and low cadmium accumulation at the molecular level.
Smart Images
Figure CN121915192B_ABST