青钱柳结合态硒多糖的分段酶解-梯级膜分离提取方法及其制得的结合态硒多糖

By employing segmented enzymatic hydrolysis and step-by-step membrane separation extraction methods, the problem of component mixing during the extraction of Cyclocarya paliurus polysaccharides in existing technologies has been solved, enabling the preparation of high-purity Cyclocarya paliurus bound selenium polysaccharides and improving the molecular weight distribution and human absorption efficiency of the product.

CN121949595BActive Publication Date: 2026-07-17HUBEI SIHUI BIOTECHNOLOGY CO LTD

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HUBEI SIHUI BIOTECHNOLOGY CO LTD
Filing Date
2026-04-03
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

In the current extraction process of Eucommia ulmoides polysaccharides or selenium-containing polysaccharides, the low molecular weight components in the front stage and the target components in the back stage enter the final product together, resulting in a wide molecular weight distribution, a high proportion of inorganic selenium, and high protein and ash content, leading to low absorption and utilization rate by the human body.

Method used

A segmented enzymatic hydrolysis-step membrane separation and extraction method was adopted. In the first stage, the low molecular weight selenium-containing component stream was separated by nanofiltration after enzymatic hydrolysis. In the second stage, the target component liquid was separated by microfiltration and ultrafiltration after enzymatic hydrolysis, followed by nanofiltration washing and ethanol precipitation to limit the molecular weight and recovery path of the target component.

Benefits of technology

It improves the molecular weight distribution concentration of the target components, reduces the proportion of inorganic selenium, enhances the polysaccharide mass fraction and human absorption and utilization rate, and obtains purer Cyclocarya paliurus-bound selenium polysaccharides.

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Abstract

本发明属于植物活性成分提取技术领域,涉及青钱柳结合态硒多糖的分段酶解‑梯级膜分离提取方法及其制得的结合态硒多糖。所述方法先对青钱柳叶原料构建第一提取体系并进行第一阶段酶解、失活和固液分离,将第一液相经纳滤膜分离形成低分子含硒组分流并排除于后续醇沉回收步骤之外;再将第一固相构建第二提取体系并进行第二阶段酶解、失活和固液分离,使第二液相依次经微滤、第一超滤、第二超滤和纳滤洗滤后实施乙醇沉淀,得到结合态硒多糖。使所得产物中目标分子量区间组分占比提高,多糖质量分数提高,无机硒比例、蛋白质量分数和灰分降低。
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