Biological cell culture sampling device for biological testing

Abstract

The present application relates to the technical field of culture sampling, and discloses a biological cell culture sampling device for biological experiments, which comprises a lower pressing shell, characterized in that a limiting ring is arranged at the top of the lower pressing shell, a top block is arranged at the top of the limiting ring, a sampling needle is slidably connected in the top block, a sealing seat is arranged at the bottom center of the top block, an outer corrugated tube is connected to the bottom of the sealing seat, an inner corrugated tube is arranged at the bottom of the sealing seat, a positive pressure cavity is formed between the inner corrugated tube and the sampling needle, a negative pressure cavity is formed between the outer corrugated tube and the inner corrugated tube, a micro positive pressure pump is arranged in the top block, and a micro vacuum pump is arranged in the top block. The double-layer telescopic corrugated tube, the sealing head, the positive and negative pressure pumps and the annular air knife are cooperated to solve the problems of "needle body exposure pollution", "needle pulling instant pollution" and "sampling process pressure imbalance" in the existing automatic sampling technology.

Description

Technical Field

[0001] This invention relates to the field of culture sampling technology, specifically to a biological cell culture sampling device for biological experiments. Background Technology

[0002] In patent application CN223576494U, the following components are sequentially connected: a culture vessel, a sampling tube, a three-way connector, a fluid delivery control component, a sample delivery tube, and a sample storage tank. The culture vessel consists of a vessel body and a cover. The cover has a first through hole adapted to the sampling tube. A sterile air drive mechanism includes a sterile air source, a first one-way valve, and a gas delivery tube. The sterile air source is connected to the three-way connector via the gas delivery tube. The first one-way valve is located on the gas delivery tube and is used to control the sterile air supply. Sterile air flows from the sterile air source to the culture tank. One end of the sampling tube extends into the through hole and enters a preset position in the culture tank. A second one-way valve is provided at one end of the sampling tube, which only allows liquid to flow from the culture tank to the outside. The other end of the sampling tube is connected to the sample delivery tube and the gas delivery tube by a three-way connector. A first pressure sensor is provided in the sampling tube. A control system is electrically or signalally connected to the first one-way valve, the second one-way valve, the fluid delivery control component, and the first pressure sensor.

[0003] The advantages are as follows: the sampling process is automated through precise control of the control system, eliminating the need for manual intervention, greatly improving sampling efficiency and reducing labor intensity. Utilizing high-precision sensors and the control system, sampling time and quantity can be precisely controlled, ensuring the accuracy and consistency of each sample and improving the reliability of experimental data. The application of a sterile air-driven mechanism and one-way valve effectively prevents the introduction of external contaminants during sampling, ensuring the purity of the culture environment and reducing the risk of contamination. By monitoring parameters such as pressure inside the culture tank in real time and feeding the data back to the control system, the system can intelligently regulate the sampling process, ensuring the smooth operation of the sampling procedure.

[0004] In the prior art, including the aforementioned patents, most commonly used one-way valves can only protect the inside of the pipeline. They cannot protect against contamination of the sampling needle when it moves in an open space and is about to be inserted into the container. Furthermore, when transferring from one culture tank to another, contaminants attached to the outer wall of the needle may fall into the next sample. Summary of the Invention

[0005] (a) Technical problems to be solved

[0006] Based on this, the purpose of the present invention is to provide a biological cell culture sampling device for biological experiments, in order to solve the technical problems that most commonly used one-way valves in the prior art can only protect the inside of the pipeline, and cannot protect against contamination of the sampling needle when it moves in an open space and is about to be inserted into the container, and when it is transferred from one culture tank to another, the contaminants attached to the outer wall of the needle may fall into the next sample.

[0007] (II) Technical Solution

[0008] To achieve the above objectives, the present invention provides the following technical solution: a biological cell culture sampling device for biological experiments, comprising a lower pressure shell, characterized in that a limiting ring is provided at the top of the lower pressure shell, a top block is provided at the top of the limiting ring, a sampling needle is slidably connected inside the top block, a sealing seat is provided at the center of the bottom end of the top block, an outer corrugated tube is connected to the bottom end of the sealing seat, an inner corrugated tube is provided at the bottom end of the sealing seat, a positive pressure cavity is formed between the inner corrugated tube and the sampling needle, a negative pressure cavity is formed between the outer corrugated tube and the inner corrugated tube, a micro positive pressure pump is provided inside the top block and cooperates with the inner corrugated tube, a micro vacuum pump is provided inside the top block and cooperates with the outer corrugated tube, a sealing head is provided at the bottom end of the outer corrugated tube and the inner corrugated tube, multiple sets of airflow pipes are provided at the bottom end of the sealing head and the airflow pipe outlets face the sampling needle, and an annular air hole that cooperates with the sampling needle is opened inside the sealing seat.

[0009] By adopting the above technical solution, the displacement robot is controlled to move above the target biological culture dish. During this entire movement, a micro positive pressure pump continuously injects filtered sterile air into the positive pressure chamber formed between the inner corrugated tube and the sampling needle through the air inlet tube, maintaining the pressure inside the chamber at a slightly positive pressure state of +Pa to +Pa. At the same time, a micro vacuum pump continuously draws air into the negative pressure chamber formed between the outer and inner corrugated tubes through the air inlet and exhaust pipes, maintaining the pressure inside the chamber at -Pa to -Pa. This "internal blowing and external suction" linkage design constructs a dynamic air-fluid isolation barrier for the sampling needle along the sampling path. The positive pressure airflow seeps outward, preventing the intrusion of external bacteria-containing air. Any trace contaminants that may cross the positive pressure air curtain are quickly captured and discharged by the outer negative pressure airflow.

[0010] Furthermore, the micro positive pressure pump fills the positive pressure chamber with sterile gas during the sampling process, and the micro vacuum pump ensures that the negative pressure chamber is kept under negative pressure during the sampling process. The limiting ring and the lower pressure shell are elastically connected by a spring, and multiple sets of locking rings are provided on the outer side of the top block.

[0011] By adopting the above technical solution, the displacement robot drive device presses down vertically as a whole. First, the soft rubber sealing ring at the bottom of the pressing shell contacts and presses against the mouth of the culture dish. As the downward pressure continues, the soft rubber sealing ring undergoes elastic deformation, closely fitting the contour of the mouth of the bottle to form a preliminary physical seal. At the same time, the micro vacuum pump increases its power and powerfully draws out any air that may remain between the soft rubber sealing ring and the mouth of the bottle through multiple sets of airflow tubes at the bottom of the sealing head. It uses negative pressure to tightly "adsorb" the air onto the mouth of the bottle. This negative pressure adsorption force can reach -N, which enhances the sealing reliability of mechanical pressing and prevents accidental failure of the seal during sampling.

[0012] Furthermore, the micro positive pressure pump is connected to the inner corrugated pipe via an inflation pipe, and the micro vacuum pump is connected to the outer corrugated pipe via an air inlet pipe and an exhaust pipe. The top of the top block is provided with a mounting cover, and a micro motor is provided inside the mounting cover. A propulsion wheel is provided at the output end of the micro motor.

[0013] By adopting the above technical solution, after the seal is established, the displacement robot continues to apply a small amount of downward pressure. The lower pressure shell and the top block move relative to each other against the elastic force of the spring, causing the double-layer bellows assembly to stretch while the sampling needle moves downward through the sealing head, piercing the sealing diaphragm of the culture dish and entering below the internal liquid surface. In the instant before piercing, the sterile air accumulated in the positive pressure chamber is ejected at high speed through the annular air hole around the sampling needle on the sealing head, forming an "annular air knife" to perform a final purging and sterilization of the needle tip that is about to enter the culture medium. This step eliminates the trace contaminants that may be brought in by the needle tip piercing the diaphragm.

[0014] Furthermore, a limiting wheel is provided at the top of the top block, and the limiting wheel cooperates with the propulsion wheel. A soft rubber sealing ring is provided at the bottom opening of the lower pressure shell. The airflow direction of the annular air hole is towards the sampling needle, and it is wider inside and narrower outside. A cavity that cooperates with the spring is opened inside the limiting ring. A rubber sealing ring that cooperates with the outer corrugated pipe and the inner corrugated pipe is provided inside the sealing seat.

[0015] By adopting the above technical solution, the pump of the external sampling system is started, and the quantitative culture medium is extracted through the sampling needle. During the extraction process, the micro positive pressure pump injects a small amount of sterile air into the positive pressure chamber according to the preset program. Part of this airflow maintains the annular air knife, and the other part is injected into the top space of the culture dish at an extremely low flow rate through the coordinated design of the airflow tube. This is a pressure compensation mechanism that can dynamically balance the pressure drop in the bottle caused by liquid extraction, and prevent the formation of excessive negative pressure in the bottle, which may damage the cells or affect the sampling flow rate.

[0016] Furthermore, the top block has a groove inside that matches the sampling needle, and the micro positive pressure pump is fixed inside the top block by a limiting plate.

[0017] By adopting the above technical solution, the micro motor on the top block is activated, driving the propulsion wheel to rotate and retracting the sampling needle upward at a constant speed. This is the "needle-pulling moment" with the highest risk of contamination. Just as the needle tip is about to retract into the sealed head, the system triggers two synchronous actions: First, the micro positive pressure pump increases its output for a short time, which instantly enhances the intensity of the "air knife" ejected from the annular air hole. The high-speed airflow can effectively blow off the residual droplets attached to the needle tip. Second, the micro vacuum pump simultaneously increases the negative pressure suction power. The blown-off droplets and any aerosols that may be generated are quickly captured and extracted by the stronger negative pressure below through the airflow pipe and negative pressure chamber before they can disperse.

[0018] (III) Beneficial Effects

[0019] Compared with the prior art, the technical solution of the present invention has the following advantages:

[0020] (1) The telescopic sheath formed by the “outer corrugated tube 16” and the “inner corrugated tube 17” completely wraps the tip of the “sampling needle 7” when not in operation. During the entire process of being carried and moved by the “displacement robot”, the “micro positive pressure pump 23” and the “micro vacuum pump 12” are activated in tandem to build a fluid isolation barrier of “inner blowing and outer suction” around the needle body. This effectively solves the problem of contamination adsorption caused by the exposure of the outer wall of the sampling needle in the prior art, and fundamentally eliminates contamination and cross-contamination of the outer wall of the needle body.

[0021] (2) At the critical moment when the “sampling needle 7” is retracted, the system triggers the linkage program: firstly, the intensity of the “annular air knife” ejected from the “annular air hole 20” is increased to blow off the needle tip droplet; at the same time, the power of the “micro vacuum pump 12” is increased, and a strong negative pressure suction field is formed through multiple sets of “air flow tubes 19” at the bottom of the “sealing head 18”, so that the pollutants stripped by the “air knife” are captured before they are dispersed and discharged through the “negative pressure chamber 21”. This mechanism overcomes the long-standing technical difficulty of “needle removal contamination”, avoids the biological hazards of bioaerosol diffusion, and also prevents the droplet from contaminating the outer wall of the culture container and other samples. (3) This scheme achieves a reliable seal with double insurance by physically pressing and sealing the "soft rubber sealing ring 11" and combining the additional negative pressure adsorption force generated by the micro vacuum pump 12 through the airflow tube 19. More importantly, during the liquid extraction process, the "micro positive pressure pump 23" will inject a small amount of sterile air into the positive pressure chamber 22 according to the program. A portion of this air is fed back to the top space of the culture dish through the "airflow tube 19" at an extremely low flow rate. This ingenious "pressure compensation mechanism" can offset the pressure loss caused by liquid extraction in real time and maintain the pressure fluctuation in the culture container within the range that the cells can tolerate. This protects the cells from negative pressure damage and ensures the stability and accuracy of the sampling flow rate. Attached Figure Description

[0022] Figure 1This is a schematic diagram of the overall structure of the present invention from a first perspective;

[0023] Figure 2 This is a schematic diagram of the overall structure of the present invention from a second perspective;

[0024] Figure 3 This is a schematic diagram of the internal structure of the present invention;

[0025] Figure 4 For the present invention Figure 3 Enlarged view of point A;

[0026] Figure 5 This is a partial structural schematic diagram of the present invention;

[0027] Figure 6 This is a partial structural diagram of the present invention;

[0028] Figure 7 This is a schematic diagram of the partial structural location of the present invention.

[0029] In the diagram: 1. Lower pressure shell; 2. Top block; 3. Limiting ring; 4. Spring; 5. Mounting cover; 6. Limiting wheel; 7. Sampling needle; 8. Sealing seat; 9. Engaging ring; 10. Propulsion wheel; 11. Soft rubber sealing ring; 12. Miniature vacuum pump; 13. Inflation pipe; 14. Inlet pipe; 15. Exhaust pipe; 16. Outer corrugated pipe; 17. Inner corrugated pipe; 18. Sealing head; 19. Airflow pipe; 20. Annular vent; 21. Negative pressure chamber; 22. Positive pressure chamber; 23. Miniature positive pressure pump. Detailed Implementation

[0030] To make the objectives, technical solutions, and advantages of the embodiments of this disclosure clearer, the technical solutions of the embodiments of this disclosure will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this disclosure. All other embodiments obtained by those skilled in the art based on the described embodiments of this disclosure without creative effort are within the scope of protection of this disclosure.

[0031] Unless otherwise defined, the technical or scientific terms used in this disclosure shall have the ordinary meaning understood by one of ordinary skill in the art to which this disclosure pertains. The terms "comprising" or "including," and similar terms used in this disclosure, mean that an element or object preceding the term encompasses the elements or objects listed following the term and their equivalents, without excluding other elements or objects. Terms such as "connected" or "linked" are not limited to physical or mechanical connections, but may also include electrical connections, whether direct or indirect. Terms such as "upper," "lower," "left," and "right" are used only to indicate relative positional relationships; these relative positional relationships may change accordingly when the absolute position of the described objects changes.

[0032] like Figures 1 to 7 As shown, the present invention provides a biological cell culture sampling device for biological experiments, comprising a lower pressure shell 1. The lower pressure shell 1 is characterized by a limiting ring 3 at its top end, a top block 2 at the top end of the limiting ring 3, a sampling needle 7 slidably connected inside the top block 2, a sealing seat 8 at the center of the bottom end of the top block 2, an outer bellows 16 connected to the bottom end of the sealing seat 8, and an inner bellows 17 at the bottom end of the sealing seat 8, forming a positive pressure chamber 2 between the inner bellows 17 and the sampling needle 7. 2. A negative pressure chamber 21 is formed between the outer corrugated pipe 16 and the inner corrugated pipe 17. A micro positive pressure pump 23 is installed inside the top block 2, and the micro positive pressure pump 23 cooperates with the inner corrugated pipe 17. A micro vacuum pump 12 is installed inside the top block 2, and the micro vacuum pump 12 cooperates with the outer corrugated pipe 16. A sealing head 18 is installed at the bottom of the outer corrugated pipe 16 and the inner corrugated pipe 17. Multiple sets of airflow pipes 19 are installed at the bottom of the sealing head 18, and the air outlets of the airflow pipes 19 face the direction of the air intake. The sampling needle 7 and the sealing seat 8 have annular air holes 20 that cooperate with the sampling needle 7. The displacement robot controls the entire movement to move above the target biological culture dish. During this movement, the micro positive pressure pump 23 continuously injects filtered sterile air into the positive pressure chamber 22 formed between the inner corrugated tube 17 and the sampling needle 7 through the air inlet tube 13, so that the pressure inside the chamber is maintained at a slightly positive pressure state of +30Pa to +50Pa. At the same time, the micro vacuum pump 12 continuously draws air into the negative pressure chamber 21 formed between the outer corrugated tube 16 and the inner corrugated tube 17 through the air inlet tube 14 and the exhaust tube 15, so that the pressure inside the chamber is maintained at -80Pa to -100Pa. This "internal blowing and external suction" linkage design constructs a dynamic air-fluid isolation barrier for the sampling needle 7 in the sampling path. The positive pressure airflow seeps outward to prevent the intrusion of external bacteria-containing air; any trace amount of contaminants that may cross the positive pressure air curtain will be quickly captured and discharged by the outer negative pressure airflow.

[0033] For example, during sampling, the micro positive pressure pump 23 fills the positive pressure chamber 22 with sterile gas, and the micro vacuum pump 12 ensures that the negative pressure chamber 21 is kept under negative pressure. The limiting ring 3 and the lower pressure shell 1 are elastically connected by the spring 4. Multiple sets of locking rings are provided on the outer side of the top block 2. The displacement robot drive device presses down vertically as a whole. First, the soft rubber sealing ring 11 at the bottom of the lower pressure shell 1 contacts and presses the mouth of the culture dish. As the downward pressure continues, the soft rubber sealing ring 11 undergoes elastic deformation and fits tightly against the contour of the mouth of the bottle, forming a preliminary physical seal. At the same time, the micro vacuum pump 12 increases its power and draws out the air that may remain between the soft rubber sealing ring 11 and the mouth of the bottle through the multiple sets of airflow tubes 19 at the bottom of the sealing head 18. The air is then quickly drawn away from the mouth of the bottle by the multiple sets of airflow tubes 19 at the bottom of the sealing head 18. The negative pressure is used to tightly "adsorb" the air onto the mouth of the bottle. The negative pressure adsorption force can reach 3-5N, which enhances the sealing reliability of the mechanical pressing and prevents the seal from failing unexpectedly during the sampling process.

[0034] For example, the micro positive pressure pump 23 is connected to the inner bellows 17 through the air inlet pipe 13, and the micro vacuum pump 12 is connected to the outer bellows 16 through the air inlet pipe 14 and the exhaust pipe 15. The top of the top block 2 is provided with a mounting cover 5, and a micro motor is provided inside the mounting cover 5. The output end of the micro motor is provided with a push wheel 10. When the seal is established, the displacement robot continues to apply a small amount of downward pressure. The lower pressure shell 1 and the top block 2 move relative to each other against the elastic force of the spring 4, so that the double bellows assembly is stretched and the sampling needle 7 is pushed down through the sealing head 18, piercing the sealing diaphragm of the culture dish and entering below the internal liquid surface. In the instant before piercing, the sterile air accumulated in the positive pressure chamber 22 is ejected at high speed through the annular air hole 20 around the sampling needle on the sealing head 18, forming an "annular air knife" to perform a final purging and sterilization of the needle tip that is about to enter the culture medium. This step eliminates the trace contaminants that may be brought in due to the needle tip piercing the diaphragm.

[0035] For example, the top of the top block 2 is provided with a limiting wheel 6, and the limiting wheel 6 cooperates with the push wheel 10. The bottom opening of the lower pressure shell 1 is provided with a soft rubber sealing ring 11. The air direction of the annular air hole 20 is towards the sampling needle 7, and it is wider inside and narrower outside. The limiting ring 3 has a cavity that cooperates with the spring 4. The sealing seat 8 is provided with a rubber sealing ring that cooperates with the outer corrugated tube 16 and the inner corrugated tube 17. When the pump of the external sampling system is started, the quantitative culture medium is extracted through the sampling needle 7. During the extraction process, the micro positive pressure pump 23 injects a small amount of sterile air into the positive pressure chamber 22 according to the preset program. Part of this airflow maintains the annular air knife, and the other part is injected into the top space of the culture dish at a very low flow rate through the coordinated design of the airflow tube 19. This is a pressure compensation mechanism that can dynamically balance the pressure drop in the bottle caused by the extraction of liquid, and prevent the formation of excessive negative pressure in the bottle to damage the cells or affect the sampling flow rate.

[0036] For example, the top block 2 has a groove inside that matches the sampling needle 7. The micro positive pressure pump 23 is fixed inside the top block 2 by a limiting plate. When the micro motor on the top block 2 is started, the propulsion wheel 10 is rotated, and the sampling needle 7 is retracted upward at a constant speed. This is the "needle removal moment" with the highest risk of contamination. Just as the needle tip is about to retract into the sealing head 18, the system triggers two synchronous actions: First, the micro positive pressure pump 23 increases its output for a short time, which instantly enhances the intensity of the "air knife" sprayed from the annular air hole 20. The high-speed airflow can effectively blow off the residual droplets attached to the needle tip. Second, the micro vacuum pump 12 simultaneously increases the negative pressure suction power. The blown-off droplets and any aerosols that may be generated are quickly captured and extracted by the stronger negative pressure below through the airflow pipe 19 and the negative pressure chamber 21 before they can disperse.

[0037] The working principle and usage process of this invention: The staff will select the biological cell culture dish to be tested, and the external displacement robot will drive the whole controlled movement so that the locking ring 9 at the end of the device is connected and locked with the limiting ring 3 around the top block 2 of the device, thereby realizing the grasping and transportation of the whole device. At this time, the device is in the initial high position, and the tail end of the sampling needle 7 is reliably connected to the external sampling tube to ensure that the subsequent liquid extraction pipeline is unobstructed.

[0038] The displacement robot moves to a position above the target biological culture dish. Throughout this movement, the micro positive pressure pump 23 continuously injects filtered sterile air into the positive pressure chamber 22 formed between the inner corrugated tube 17 and the sampling needle 7 through the air inlet tube 13, maintaining the pressure inside the chamber at a slightly positive pressure state of +30Pa to +50Pa. Simultaneously, the micro vacuum pump 12 continuously draws air into the negative pressure chamber 21 formed between the outer corrugated tube 16 and the inner corrugated tube 17 through the air inlet tube 14 and the exhaust tube 15, maintaining the pressure inside the chamber at -80Pa to -100Pa. This "internal blowing and external suction" linkage design constructs a dynamic air-fluid isolation barrier for the sampling needle 7 along the sampling path. The positive pressure airflow seeps outward, preventing the intrusion of external bacteria-containing air. Any trace contaminants that may cross the positive pressure air curtain are quickly captured and discharged by the outer negative pressure airflow.

[0039] The displacement robot drive device presses down vertically. First, the soft rubber sealing ring 11 at the bottom of the pressing shell 1 contacts and presses against the mouth of the culture dish. As the downward pressure continues, the soft rubber sealing ring 11 undergoes elastic deformation, closely fitting the contour of the mouth of the bottle to form a preliminary physical seal. At the same time, the micro vacuum pump 12 increases its power and powerfully draws out any air that may remain between the soft rubber sealing ring 11 and the mouth of the bottle through multiple sets of airflow tubes 19 at the bottom of the sealing head 18. It uses negative pressure to tightly "adsorb" the air onto the mouth of the bottle. This negative pressure adsorption force can reach 3-5N, which enhances the sealing reliability of the mechanical pressing and prevents accidental failure of the seal during the sampling process.

[0040] Once the seal is established, the displacement robot continues to apply a small amount of downward pressure. The lower pressure shell 1 and the top block 2 move relative to each other against the elastic force of the spring 4, causing the double-layer bellows assembly to stretch. At the same time, the sampling needle 7 moves downward through the sealing head 18, piercing the sealing diaphragm of the culture dish and entering below the internal liquid surface. Just before piercing, the sterile air accumulated in the positive pressure chamber 22 is ejected at high speed through the annular air hole 20 surrounding the sampling needle on the sealing head 18, forming an "annular air knife" to perform a final purging and sterilization of the needle tip that is about to enter the culture medium. This step eliminates any trace contaminants that may be brought in due to the needle tip piercing the diaphragm.

[0041] Subsequently, the pump of the external sampling system is activated, and the quantitative culture medium is extracted through the sampling needle 7. During the extraction process, the micro positive pressure pump 23 injects a small amount of sterile air into the positive pressure chamber 22 according to the preset program. Part of this airflow maintains the annular air knife, and the other part is injected into the top space of the culture dish at an extremely low flow rate through the coordinated design of the airflow tube 19. This is a pressure compensation mechanism that can dynamically balance the pressure drop in the bottle caused by liquid extraction, and prevent the formation of excessive negative pressure in the bottle, which may damage the cells or affect the sampling flow rate.

[0042] After sampling is completed, the micro motor on the top block 2 is started, which drives the propulsion wheel 10 to rotate and retract the sampling needle 7 upward at a constant speed. This is the "needle removal moment" with the highest risk of contamination. Just as the needle tip is about to retract into the sealing head 18, the system triggers two synchronous actions: First, the micro positive pressure pump 23 increases its output for a short time, which instantly enhances the intensity of the "air knife" sprayed from the annular air hole 20. The high-speed airflow can effectively blow off the residual droplets attached to the needle tip; Second, the micro vacuum pump 12 simultaneously increases the negative pressure suction power. The blown-off droplets and any aerosols that may be generated are quickly captured and extracted by the stronger negative pressure below through the airflow pipe 19 and the negative pressure chamber 21 before they can disperse.

[0043] After the sampling needle 7 is fully retracted, the entire displacement robot lifting device is reset under the action of the spring 4, and can be moved to the next work station or cleaning station in a controlled manner. In the cleaning station, cleaning fluid and disinfectant can be injected into the positive pressure chamber 22 and the negative pressure chamber 21 respectively, and turbulence can be formed by utilizing their internal structure to achieve thorough cleaning and disinfection of the entire needle channel and the inner wall of the sheath. This design makes subsequent maintenance more thorough and avoids the accumulation of contaminants.

[0044] The above embodiments are merely exemplary embodiments of the present invention and are not intended to limit the present invention. The scope of protection of the present invention is defined by the claims. Those skilled in the art can make various modifications or equivalent substitutions to the present invention within its spirit and scope of protection, and such modifications or equivalent substitutions should also be considered to fall within the scope of protection of the present invention.

Claims

1. A biological cell culture sampling device for biological experiments, comprising a lower pressure shell (1), characterized in that, The top of the lower pressure shell (1) is provided with a limiting ring (3), and the top of the limiting ring (3) is provided with a top block (2). A sampling needle (7) is slidably connected inside the top block (2). A sealing seat (8) is provided at the center of the bottom end of the top block (2). An outer corrugated tube (16) is connected to the bottom end of the sealing seat (8). An inner corrugated tube (17) is provided at the bottom end of the sealing seat (8). A positive pressure cavity (22) is formed between the inner corrugated tube (17) and the sampling needle (7). A negative pressure cavity (21) is formed between the outer corrugated tube (16) and the inner corrugated tube (17). A micro positive pressure pump (23) is provided inside the top block (2), and the micro positive pressure pump (23) cooperates with the inner corrugated tube (17). During the sampling process, the micro positive pressure pump (23)... Sterile gas is introduced into the positive pressure chamber (22). A micro vacuum pump (12) is installed inside the top block (2), and the micro vacuum pump (12) cooperates with the outer corrugated pipe (16). The micro vacuum pump (12) ensures that the negative pressure chamber (21) is kept under negative pressure during the sampling process. A sealing head (18) is provided at the bottom of the outer corrugated pipe (16) and the inner corrugated pipe (17). Multiple sets of airflow pipes (19) are provided at the bottom of the sealing head (18), and the air outlet of the airflow pipe (19) faces the sampling needle (7). An annular air hole (20) that cooperates with the sampling needle (7) is opened inside the sealing seat (8). A soft rubber sealing ring (11) is provided at the bottom opening of the lower pressure shell (1). The airflow direction of the annular air hole (20) is towards the sampling needle (7), and it is wider inside and narrower outside.

2. The biological cell culture sampling device for biological experiments according to claim 1, characterized in that: The limiting ring (3) and the lower pressure shell (1) are elastically connected by a spring (4), and multiple sets of locking rings (9) are provided on the outer side of the top block (2).

3. The biological cell culture sampling device for biological experiments according to claim 1, characterized in that: The micro positive pressure pump (23) is connected to the inner corrugated pipe (17) through an air inlet pipe (13), and the micro vacuum pump (12) is connected to the outer corrugated pipe (16) through an air inlet pipe (14) and an air outlet pipe (15).

4. The biological cell culture sampling device for biological experiments according to claim 1, characterized in that: The top of the top block (2) is provided with a mounting cover (5), and a micro motor is provided inside the mounting cover (5). A propulsion wheel (10) is provided at the output end of the micro motor.

5. A biological cell culture sampling device for biological experiments according to claim 4, characterized in that: The top of the top block (2) is provided with a limiting wheel (6), and the limiting wheel (6) cooperates with the propulsion wheel (10).

6. The biological cell culture sampling device for biological experiments according to claim 1, characterized in that: The limiting ring (3) and the lower pressure shell (1) are elastically connected by a spring (4). The limiting ring (3) has a cavity that cooperates with the spring (4). The sealing seat (8) has a rubber sealing ring that cooperates with the outer bellows (16) and the inner bellows (17).

7. The biological cell culture sampling device for biological experiments according to claim 1, characterized in that: The top block (2) has a groove inside that matches the sampling needle (7), and the micro positive pressure pump (23) is fixed inside the top block (2) by a limiting plate.

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