RNA polymerase variants and uses thereof
CN121950748BActive Publication Date: 2026-06-09NANJING VAZYME BIOTECH CO LTD
Patent Information
- Application Number
- CN202610417105.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2026-04-01
- Publication Date
- 2026-06-09
- Estimated Expiration
- 2046-04-01
AI Technical Summary
Technical Problem
The existing T7 RNA polymerase has low catalytic efficiency, resulting in high RNA production costs, and the integrity and yield of RNA products need to be improved.
Method used
A class of RNA polymerase variants was developed with an amino acid sequence that is at least 95% identical to that of wild-type T7 RNA polymerase and contains specific amino acid site mutations, such as Q107, E108, I109, M369, N370, I587 or K700, which improve the enzyme’s catalytic efficiency and the integrity of the RNA product.
Benefits of technology
RNA polymerase variants significantly improved catalytic efficiency, reduced enzyme dosage, lowered production costs, and improved RNA product integrity and yield.
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Smart Images
Figure CN121950748B_ABST
Abstract
The application provides an RNA polymerase variant and application thereof, and relates to the technical field of biology. The application obtains a T7 RNA polymerase variant by modifying a wild-type T7 RNA polymerase, the enzyme specific activity of the T7 RNA polymerase variant is significantly improved compared with that of the wild-type T7 RNA polymerase, the addition amount of the polymerase in the in-vitro transcription reaction process can be reduced, and the cost is saved; and the mutant also effectively reduces the generation of incomplete fragment impurities, and significantly improves the mRNA integrity to 99%. The application also provides an in-vitro transcription method for preparing RNA.
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Citation Information
Patent Citations
RNA polymerase variants and uses thereof
CN120905182A
T7 RNA polymerase variants for RNA synthesis
US20250019675A1