β-葡萄糖醛酸苷酶突变体及其应用
By introducing the T512S mutation at the Thr 512 site of β-glucuronidase AtGUS(-3t), the catalytic efficiency was improved, solving the problems of low catalytic efficiency and high production cost in the existing technology, and realizing the efficient preparation of glycyrrhizic acid derivatives.
CN121950758BActive Publication Date: 2026-07-17BEIJING CITY UNIVERSITY
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- BEIJING CITY UNIVERSITY
- Filing Date
- 2026-03-31
- Publication Date
- 2026-07-17
AI Technical Summary
Technical Problem
Existing β-glucuronidases have low catalytic efficiency and high production costs, making it difficult to efficiently prepare glycyrrhizic acid derivatives GAMG and GA.
Method used
By introducing mutations, particularly the T512S mutation, at the Thr 512 site of β-glucuronidase AtGUS(-3t), catalytic efficiency was improved, forming a β-glucuronidase mutant that enhanced the substrate recognition ability of the catalytic active site.
Benefits of technology
The mutant enzyme exhibited a 13.92-fold increase in catalytic efficiency, significantly reducing production costs and promoting the synthesis and application of glycyrrhizic acid derivatives.
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Figure CN121950758B_ABST
Abstract
本发明公开了β‑葡萄糖醛酸苷酶突变体及其应用，属于基因工程和蛋白质工程改造技术领域。本发明公开的β‑葡萄糖醛酸苷酶突变体，在SEQ ID NO.3所示氨基酸序列的基础上，存在下述突变：T512S或T512A。β‑葡萄糖醛酸苷酶突变体T512S的催化效率相较于AtGUS(‑3t)提高13.92倍，展现出显著的工业化应用潜力。
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