一种调控耐盐性的紫花苜蓿MsCML69基因及其应用
By cloning and overexpressing the MsCML69 gene of alfalfa, constructing a recombinant expression vector and transforming alfalfa, the problem of insufficient salt tolerance of alfalfa was solved, and salt tolerance was significantly improved.
CN121975820BActive Publication Date: 2026-07-17ZHEJIANG FORESTRY UNIVERSITY
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Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- ZHEJIANG FORESTRY UNIVERSITY
- Filing Date
- 2026-04-07
- Publication Date
- 2026-07-17
AI Technical Summary
Technical Problem
Existing technologies are insufficient to effectively improve the salt tolerance of alfalfa, thus limiting its growth and yield.
Method used
The MsCML69 gene of alfalfa was cloned and overexpressed, and the recombinant expression vector pCAMBIA3301-MsCML69 was constructed. Alfalfa was then transformed using Agrobacterium-mediated transformation to improve its salt tolerance.
Benefits of technology
A highly efficient molecular breeding technology scheme for salt tolerance improvement was established to significantly improve the salt tolerance of alfalfa.
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Abstract
本发明公开了一种调控耐盐性的紫花苜蓿MsCML69基因及其应用。MsCML69基因的核苷酸序列如SEQ ID NO.1所示；其对应的氨基酸序列如SEQ ID NO.2所示。本发明从紫花苜蓿中克隆了类钙调蛋白基因MsCML69的cDNA序列，并将获得的MsCML69基因与植物表达载体连接，构建了重组表达载体，用构建的重组表达载体转化植物叶片，最后培育成转基因植株。结果表明该基因过表达能够使植株耐盐性显著提高。MsCML69基因的发现为紫花苜蓿抗逆新种质的培育提供基因资源，同时提高其适应范围，该基因的应用具有重要的生产实践意义。
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