一种基于优化肽接头的多价CD301融合蛋白、功能化磁珠及其在CTC捕获中的应用
By optimizing the peptide linker design of the multivalent CD301 fusion protein, the problems of EpCAM-dependent missed detection and insufficient stability in existing CTC capture technologies have been solved, achieving efficient and stable capture of EMT-type CTCs, with significantly improved capture efficiency and stability.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- CHONGQING ZHENJIAYI BIOTECHNOLOGY CO LTD
- Filing Date
- 2026-04-09
- Publication Date
- 2026-07-17
AI Technical Summary
Existing CTC capture technologies that rely on EpCAM antibodies have the problem of missing interstitial CTCs. Furthermore, CD301 monomers have weak affinity, complex polymerization design, and poor linker design, leading to instability in the blood environment.
By employing an optimized peptide linker design, multiple CD301 CRD domains are tandemly linked through genetic engineering and connected using flexible linkers (GGGGS) and rigid linkers (hCGβ CTP) to achieve multivalent chelation effects and enhance stability in the blood environment.
It achieves efficient capture of CTCs with different phenotypes, especially EMT-type CTCs, with a capture efficiency of up to 92.8%. It also significantly improves stability in the blood environment, prolongs the half-life, and avoids protease degradation.
Smart Images
Figure CN121991252B_ABST