一种甲醇利用菌的培养方法

By employing a multi-stage culture method, utilizing glucose and methanol as carbon sources, and combining them with ammonium salts and nitrates as inorganic nitrogen sources, the metabolic pathways of the bacteria were regulated, thus solving the problem of low methanol protein synthesis efficiency. This enabled the efficient conversion of methanol into bacterial protein and improved the metabolic efficiency of the bacteria.

CN121991827BActive Publication Date: 2026-07-17BEIJING DELIANGYUAN ENVIRONMENTAL TECH CO LTD +1

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
BEIJING DELIANGYUAN ENVIRONMENTAL TECH CO LTD
Filing Date
2026-02-14
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

In existing methods for cultivating methanol-utilizing bacteria, the efficiency of methanol protein synthesis is low, and the methanol metabolic flux is difficult to convert into bacterial protein efficiently and directionally, resulting in significant carbon loss.

Method used

A multi-stage culture method was adopted, using glucose and methanol as carbon sources, combined with ammonium salts and nitrates as inorganic nitrogen sources. By adjusting the ratio of carbon and nitrogen sources, and by adding phosphates and trace elements, the metabolic pathway of the bacteria was controlled to achieve efficient methanol synthesis.

Benefits of technology

It improved the synthesis efficiency of methanol protein, reduced the loss of carbon source, realized the efficient conversion of methanol into bacterial protein, and enhanced the metabolic efficiency of bacteria.

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Abstract

本发明提供一种甲醇利用菌的培养方法,包括如下步骤:取食葡萄糖食甲基菌,接种于活化培养基中,培养得到活化菌液;将活化菌液,接种于一级种子培养基中,培养得到一级种子液;将一级种子液,接种于二级种子培养基中,培养得到二级种子液;将二级种子液,接种于发酵培养基中,调整转速使初始溶氧值为15‑25%,通气量为1.2‑1.8vvm,发酵至溶氧值上升15%以上,补加含甲醇的补料培养基,并使发酵培养基中的甲醇浓度为1.5‑2.0%(v / v),发酵即得。本发明的甲醇利用菌的培养方法可高效合成甲醇蛋白。
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