一种富集血浆低丰度蛋白的纳米粒子、其制备方法与癌症早筛相关应用
By using hyperbranched polyethyleneimine-modified superparamagnetic Fe3O4 nanoparticles and 2-iminothionyl hydrochloride-modified targeted antibodies, the problem of efficient enrichment of low-abundance proteins in plasma was solved, achieving high-sensitivity and high-accuracy early cancer screening and improving the effectiveness of early cancer detection.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- CHANGCHUN INSTITUTE OF APPLIED CHEMISTRY CHINESE ACADEMY OF SCIENCES
- Filing Date
- 2026-04-08
- Publication Date
- 2026-07-17
AI Technical Summary
Existing technologies struggle to achieve high sensitivity and accuracy in enriching low-abundance proteins in plasma. Single polymer-modified materials lack sufficient non-specific enrichment sensitivity, and single-target antibody-modified materials cannot provide comprehensive disease-related proteomics information.
Superparamagnetic Fe3O4 nanoparticles modified with hyperbranched polyethyleneimine are combined with targeted antibodies modified with 2-iminothione hydrochloride. High-density, activity-preserving antibody modification is achieved on the nanoparticle surface through click reaction or thiol exchange reaction, resulting in broad-spectrum and specific capture.
It achieves high sensitivity and high accuracy enrichment of low-abundance proteins in plasma, significantly improving the early detection rate and accuracy of cancer. Combined with mass spectrometry analysis and deep learning models, it improves the efficiency and accuracy of early cancer screening.
Smart Images
Figure CN122006676B_ABST