Microfluidic organ-on-chip and systems, applications thereof and methods of multi-organ co-culturing

Abstract

The application relates to the technical field of biological tissue engineering, and discloses a microfluidic organ chip, which comprises a chip body provided with a plurality of culture modules. Each culture module comprises a culture zone comprising a first culture zone and a second culture zone in communication, two first liquid storage pools in communication with the first culture zone respectively, two second liquid storage pools in communication with the second culture zone respectively, and a porous membrane arranged between the first culture zone and the second culture zone to realize material exchange. The culture zone, the two first liquid storage pools and the two second liquid storage pools of each culture module are arranged in correspondence with arrayed well positions of a well plate. The arrangement of each culture module in correspondence with the arrayed well positions of the well plate can seamlessly dock a high-throughput detection device, and the microfluidic organ chip can be directly applied to large-scale drug screening, drug efficacy evaluation and the like. The microfluidic organ chip can realize gravity-driven or pump-driven perfusion culture, and can also realize single-organ co-culture and multi-organ co-culture. The application further discloses a microfluidic organ chip system, application of the microfluidic organ chip system and a multi-organ co-culture method.

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