一种漆酶及其应用
By using database mining and E. coli recombinant expression technology, the amino acid sequence of laccase Lac-11 was optimized, which solved the problems of insufficient activity and poor stability of existing laccases, and achieved efficient treatment of dye wastewater and degradation of lignin, thereby reducing production costs.
CN122012426BActive Publication Date: 2026-07-17HANGZHOU LUCA INTELLIGENT TECHNOLOGY CO LTD
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- HANGZHOU LUCA INTELLIGENT TECHNOLOGY CO LTD
- Filing Date
- 2026-04-15
- Publication Date
- 2026-07-17
AI Technical Summary
Technical Problem
Existing laccases suffer from insufficient activity, poor stability, and high production costs, making it difficult to meet the needs of industrial applications.
Method used
The highly active laccase candidate protein Lac-11 was obtained through database mining. It was recombinantly expressed in E. coli and subjected to affinity chromatography. The obtained laccase Lac-11 was fused with a His tag, and its amino acid sequence was optimized to improve enzyme activity and stability.
Benefits of technology
Lac-11 exhibits 50% higher enzyme activity, maintains high activity over a wide pH range, and demonstrates superior thermal stability compared to existing enzymes. It can efficiently treat dye wastewater and degrade lignin, thereby reducing production costs.
✦ Generated by Eureka AI based on patent content.
Smart Images
Figure CN122012426B_ABST
Abstract
本发明公开了一种漆酶及其应用，属于生物工程领域。本发明通过数据库挖掘获得高活性漆酶Lac‑11，相比现有高活性漆酶BstCotA，具有以下有益效果：1.酶活显著提升，37℃、pH 5.0条件下酶活提高近50%，降低工业用酶成本。2.pH稳定性更强，pH 5.0‑8.0范围内酶活保留率更高，适应更广酸碱环境。3.热稳定性更优，50‑80℃中高温区间稳定性显著高于对照酶，适配工业高温工艺。4.染料脱色效率更高，对氨基黑10B、结晶紫脱色率分别达31%、37%，可高效处理印染废水。5.木质素降解能力突出，1小时、2小时、24小时降解率分别为22%、25%和58%，利于制浆漂白与生物质资源化。
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