High-efficiency germination culture method for breaking deep dormancy of tilia mume seed embryo

Abstract

The present application relates to the technical field of plant tissue culture and endangered tree species breeding, in particular to a high-efficiency germination culture method for breaking the deep dormancy characteristics of Tilia amurensis seed embryo, which is used to solve the problems of low germination rate, long cycle, high pollution rate and poor embryo quality of the existing Tilia amurensis seed propagation. The method comprises the following steps: selecting a healthy Tilia amurensis mother tree in a specific area with an east longitude of 129°19'±0.5°, and collecting seeds with a moisture content of 45.25%-59.06% from August 29 to September 20; pretreating and removing inhibition by soaking in 50mg / L GA3 for 12h after 4℃ low-temperature stratification for 20d; precise disinfection with 75% alcohol and 2% sodium hypochlorite; making a 1-2mm incision on the seed coat and then treating with a vitamin C-humic acid solution for antioxidation; inoculating in an optimized MS culture medium containing 0.1mg / L 6-BA and other hormones, and then culturing in darkness at 22℃ for 5d, and then culturing under 24℃ and 2000lx illumination. The method can efficiently break the deep dormancy, has a low pollution rate, and the embryo seedling is healthy, which can support the large-scale breeding and population restoration of Tilia amurensis.