Protein imprinting polymer based on organic framework nanoszyme, preparation method and application

Abstract

The present application relates to the technical field of protein imprinting polymer, and particularly relates to a protein imprinting polymer based on an organic framework nanometer enzyme, a preparation method and application.The preparation method is characterized by a strategy of pre-assembly and subsequent positioning initiation, which makes free radicals mainly generated in a microenvironment around a template molecule, realizes in-situ positioning initiation of a polymerization reaction, and thus reduces invalid polymerization;an iron-based or zinc-based MOF is introduced to replace a natural enzyme active chemical initiator, which not only can ensure high reproducibility and controllability of a polymerization process, but also enables a product to maintain recognition performance in a complex environment and after multiple adsorption-desorption cycles, and reduces long-term application cost;at the same time, the natural conformation and activity of the template protein can be maximally retained, and the fidelity of the imprinting cavity is ensured.The organic framework nanometer enzyme initiation system constructed in the present application is a universal platform, and provides a new way for directional design and preparation of a separation medium or a sensing and recognition element with high selectivity and high affinity.

Images