A glycosyltransferase ilugt88a3 and its application in flavonoid glycoside synthesis

Abstract

The application discloses a glycosyltransferase IlUGT88A3 and application thereof in flavonoid glycoside synthesis, and belongs to the technical field of genetic engineering and enzyme engineering. The glycosyltransferase IlUGT88A3 can specifically catalyze glucosylation and galactosylation of a hydroxyl group at a C3 position of quercetin, and efficiently synthesize isoquercitrin and hyperoside. The optimum pH of the glycosyltransferase IlUGT88A3 is 7.0-7.5, and the optimum temperature is 30 DEG C, and the reaction condition is mild; the glycosyltransferase IlUGT88A3 has good substrate promiscuity, can utilize sugar donors such as UDP-glucose, UDP-galactose, UDP-rhamnose and UDP-xylose, and can perform site-specific glycosylation modification on various flavonoid receptors. The application fills the blank of a lack of flavone 3-O-galactosyltransferase resources, has strong catalytic specificity, high efficiency and pure product, and can be used for in-vitro enzyme synthesis and plant heterologous synthesis of high-value flavonoid glycosides such as hyperoside, and provides a core enzyme element and technical support for green and efficient preparation of medical raw materials.

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