一种基因编辑改良鲜食大豆品质的靶点组合及其应用
By designing a combination of sgRNAs targeting two specific sites to target the LOX and BADH homologous genes in fresh soybeans, simultaneous editing of multiple genes was achieved, solving the problems of high breeding costs and long cycles, and obtaining efficient and stable quality improvement results.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- SHUNFENG BIOTECHNOLOGY (HAINAN) CO LTD
- Filing Date
- 2026-04-17
- Publication Date
- 2026-07-17
AI Technical Summary
Existing technologies make it difficult to efficiently and simultaneously edit the five genes of fresh soybeans, resulting in high breeding costs, long cycles, and off-target risks, making it difficult to meet the requirements for off-season planting and diversified quality.
We designed a combination of sgRNAs containing two targets to target the highly conserved core domains of three LOX homologous genes and two BADH homologous genes in fresh soybean. We then used the CRISPR/Cas9 gene editing vector to achieve simultaneous editing of multiple genes, simplifying the vector construction process and reducing breeding costs.
It achieves a simultaneous mutation efficiency of over 80% for 5 genes, significantly reducing breeding costs, shortening the breeding cycle, and obtaining high-quality fresh soybeans with no beany smell and enhanced aroma. It is suitable for stable and efficient breeding with different genetic backgrounds.
Smart Images
Figure CN122038408B_ABST