BoTHL1基因或其编码蛋白在提高青花菜自交亲和性和 / 或结籽率中的应用、育种方法和重组载体
By overexpressing the BoTHL1 gene in broccoli and using recombinant vectors and Agrobacterium-mediated transformation, the problems of self-incompatibility and low seed setting rate in broccoli were solved, achieving improved self-compatibility and increased seed setting rate, providing an efficient solution for cruciferous crop breeding.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- HUNAN AGRI UNIV
- Filing Date
- 2026-04-15
- Publication Date
- 2026-07-17
AI Technical Summary
In broccoli breeding, the problems of self-incompatibility and low seed setting rate are addressed by traditional breeding methods, which are cumbersome and costly. Knocking out SRK or SCR genes may lead to decreased pollen viability or non-specific recognition defects.
By overexpressing the BoTHL1 gene or its encoded protein, the pCAMBIA1301-35S-EGFP plant expression vector was introduced into broccoli plants to increase the expression level of the BoTHL1 gene. The recombinant vector was cloned and explants were transformed using Agrobacterium-mediated transformation. Positive plants were screened and the expression level of BoTHL1 was verified. Self-pollination was then achieved to improve self-compatibility and seed setting rate.
Without affecting pollen development, it specifically enhances the stigma's ability to accept its own pollen, removes the self-incompatibility barrier, significantly improves the self-pollination seed setting rate, provides high-quality germplasm resources, overcomes self-incompatibility under natural conditions, and is suitable for breeding cruciferous crops.
Smart Images
Figure CN122038466B_ABST