Method and system for evaluating hydrophilic properties of hydrophilic membrane materials in electrochemical test strips

Abstract

The present disclosure relates to the technical field of detection, and particularly relates to a detection method and system for evaluating the hydrophilic performance of a hydrophilic membrane material in an electrochemical test paper, which can capture the dynamic change of a test liquid in the hydrophilic membrane, so as to accurately and quantitatively evaluate the hydrophilic performance of the hydrophilic membrane. The method comprises the following steps: according to the design requirements of the structure of the electrochemical test paper, processing the hydrophilic membrane into a target test paper, the target test paper comprising an insulating substrate, a first electrode and a second electrode, one end of the target test paper being provided with a sample inlet for contacting the test liquid; applying a detection voltage to the first electrode and the second electrode of the target test paper; contacting the target test liquid with the sample inlet of the target test paper, and monitoring the time interval between the sudden change from the no-current signal to the current signal between the first electrode and the second electrode of the target test paper; and obtaining the evaluation result of the hydrophilic performance of the hydrophilic membrane according to the time interval.

Description

Technical Field

[0001] This disclosure relates to the field of detection technology, specifically to a detection method and system for evaluating the hydrophilic properties of hydrophilic membrane materials in electrochemical test paper. Background Technology

[0002] In the field of in vitro diagnostics (IVD), especially in point-of-care testing (POCT), electrochemical methods are widely used due to their advantages such as high detection sensitivity, short reaction time, and ease of operation. Electrochemical test strips, as electrochemical biosensors, can be used to detect indicators such as blood glucose, uric acid, blood lipids, and blood ketones.

[0003] As one of the core raw materials in electrochemical test strips, the hydrophilic membrane plays a crucial role. The hydrophilic membrane is made by coating a layer of adhesive onto a polyethylene terephthalate (PET) film, giving the membrane a hydrophilic surface. This allows the sample absorption channels on the electrochemical test strip to create a capillary effect, drawing samples (such as blood, urine, sweat, etc.) into the reaction area. The hydrophilicity of hydrophilic membranes varies among different manufacturers, and this directly affects the sample absorption speed, thus influencing the overall performance of the electrochemical test strip. Therefore, accurately and quantitatively assessing the hydrophilicity of the membrane is key to determining the performance of the electrochemical test strip. Summary of the Invention

[0004] To overcome the problems existing in the related technologies, this disclosure provides a detection method and system for evaluating the hydrophilic properties of hydrophilic membrane materials in electrochemical test paper, so as to accurately and quantitatively evaluate the hydrophilic properties of the hydrophilic membrane.

[0005] According to a first aspect of the present disclosure, a method for evaluating the hydrophilic properties of a hydrophilic membrane material in an electrochemical test paper is provided, comprising:

[0006] According to the structural design requirements of electrochemical test paper, the hydrophilic membrane is processed into a target test paper. The target test paper includes an insulating substrate, a first electrode and a second electrode disposed on the insulating substrate at a predetermined distance, and the hydrophilic membrane covering the first electrode and the second electrode and bonded to the insulating substrate with double-sided adhesive to form a closed channel. One end of the target test paper is provided with a sample inlet channel for contacting the test liquid.

[0007] A detection voltage is applied to the first and second electrodes of the target test strip;

[0008] The target test solution is brought into contact with the sample injection channel of the target test strip, and the time interval between the first and second electrodes of the target test strip from no current signal to a sudden change in current signal is monitored.

[0009] The hydrophilic properties of the hydrophilic membrane are evaluated based on the time interval.

[0010] According to a second aspect of the present disclosure, a detection system for evaluating the hydrophilic properties of a hydrophilic membrane material in an electrochemical test strip is provided, comprising a processing device and a target test strip processed from a hydrophilic membrane to be tested according to the structural design requirements of the electrochemical test strip. The target test strip includes an insulating substrate, a first electrode and a second electrode disposed on the insulating substrate at a predetermined distance, and the hydrophilic membrane covering the first electrode and the second electrode and bonded to the insulating substrate by double-sided adhesive to form a closed channel. One end of the target test strip is provided with a sample inlet channel for contacting a test liquid.

[0011] The target test strip is used to contact the target test liquid through the sample introduction channel;

[0012] The processing device is used to apply a detection voltage to the first and second electrodes of the target test strip. After the target test liquid comes into contact with the sample injection channel of the target test strip, it monitors the time interval between the first and second electrodes of the target test strip from no current signal to a sudden change in current signal. Based on the time interval, it obtains the hydrophilic performance evaluation result of the hydrophilic membrane.

[0013] The technical solutions provided by the embodiments of this disclosure may include the following beneficial effects:

[0014] The detection method for evaluating the hydrophilic properties of hydrophilic membrane materials in electrochemical test strips provided in this disclosure transforms hydrophilicity assessment from surface physicochemical measurement to fluid dynamics performance measurement. By monitoring the time interval between the abrupt change from no current signal to a current signal between the first and second electrodes of the target test strip, a quantitative assessment of the hydrophilic properties of the membrane material is achieved. The method is simple to operate and requires no complex calculations. Furthermore, the time interval between the abrupt change from no current signal to a current signal between the first and second electrodes of the target test strip reflects the dynamic flow of the test liquid within the target test strip, thereby capturing the dynamic changes of the test liquid within the hydrophilic membrane. Compared to static surface measurement methods (such as the water contact angle method) in related technologies, this method improves the accuracy of hydrophilicity assessment results. Attached Figure Description

[0015] The accompanying drawings, which are incorporated in and form a part of this specification, illustrate embodiments consistent with this disclosure and, together with the description, serve to explain the principles of this disclosure.

[0016] Figure 1 This is a flowchart illustrating an exemplary embodiment of the present disclosure of a detection method for evaluating the hydrophilic properties of a hydrophilic membrane material in an electrochemical test paper;

[0017] Figure 2 This is a schematic diagram of the structure of a target test strip shown in an exemplary embodiment of the present disclosure;

[0018] Figure 3 This is a schematic diagram of the structure of a detection system for evaluating the hydrophilic properties of a hydrophilic membrane material in an electrochemical test paper, as shown in an exemplary embodiment of this disclosure. Detailed Implementation

[0019] Exemplary embodiments will now be described in detail, examples of which are illustrated in the accompanying drawings. When the following description relates to the drawings, unless otherwise indicated, the same numerals in different drawings denote the same or similar elements. The embodiments described in the following exemplary embodiments do not represent all embodiments consistent with this disclosure. Rather, they are merely examples of apparatuses and methods consistent with some aspects of this disclosure.

[0020] The terminology used in this disclosure is for the purpose of describing particular embodiments only and is not intended to be limiting of the disclosure. The singular forms “a,” “the,” and “the” used in this disclosure are also intended to include the plural forms unless the context clearly indicates otherwise. It should be understood that although the terms first, second, third, etc., may be used in this disclosure to describe various information, such information should not be limited to these terms. These terms are used only to distinguish information of the same type from one another. For example, first information may also be referred to as second information without departing from the scope of this disclosure, and similarly, second information may also be referred to as first information.

[0021] Hydrophilicity is the degree of interaction between a substance and water molecules. Substances with higher hydrophilicity have better wettability and wet adhesion. Common methods for evaluating hydrophilicity in related technologies include water contact angle measurement, coating properties testing, and surface free energy testing.

[0022] The water contact angle method determines the hydrophilicity / hydrophobicity of a thin film surface by measuring the size of the water contact angle. A smaller contact angle indicates a more hydrophilic material, while a larger contact angle indicates a more hydrophobic material. However, this method can be affected by surface roughness or contamination, leading to inaccurate measurements. Furthermore, static measurements cannot capture dynamic changes, thus affecting the accuracy of the hydrophilicity assessment results.

[0023] The coating performance test assesses the hydrophilicity of a substance by measuring how well a coating liquid spreads on its surface. Substances with good coating performance can be coated quickly and evenly onto the surface, while substances with poor coating performance tend to form beads. However, this method is highly subjective, the results depend on the operator's experience, and lacks quantitative standards.

[0024] Surface free energy (SFE) measurement assesses a substance's hydrophilicity by calculating its surface free energy based on the contact angles between the substance's surface and different liquids. A higher SFE indicates a more hydrophilic substance. However, this method requires complex calculations, and SFE is a comprehensive parameter that may not distinguish the specific contributions of polar and nonpolar components to hydrophilicity. For example, a high SFE might originate from nonpolar interactions rather than true hydrophilicity.

[0025] Based on this, in a first aspect, at least one embodiment of the present disclosure provides a detection method for evaluating the hydrophilic properties of a hydrophilic membrane material in an electrochemical test paper, so as to accurately and quantitatively evaluate the hydrophilic properties of the hydrophilic membrane.

[0026] Please refer to the appendix. Figure 1 The present disclosure illustrates the flow chart of a detection method for evaluating the hydrophilic properties of hydrophilic membrane materials in electrochemical test strips, including steps S101 to S104.

[0027] In step S101, the hydrophilic membrane is processed into a target test strip according to the structural design requirements of the electrochemical test strip. The target test strip includes an insulating substrate, a first electrode and a second electrode spaced at a predetermined distance on the insulating substrate, and the hydrophilic membrane covering the first and second electrodes and bonded to the insulating substrate with double-sided adhesive to form a sealed channel. One end of the target test strip has a sample inlet channel for contacting the test solution.

[0028] In step S102, a detection voltage is applied to the first and second electrodes of the target test paper.

[0029] In step S103, the target test solution is brought into contact with the sample inlet channel of the target test strip, and the time interval between the first electrode and the second electrode of the target test strip from no current signal to the sudden change of current signal is monitored.

[0030] In step S104, the hydrophilic performance evaluation results of the hydrophilic membrane are obtained according to the time interval.

[0031] For example, a certain voltage is applied to the first and second electrodes, ranging from 0.05 to 1V, with an optimal voltage of 0.4V. When the test liquid has not reached the second electrode, the first and second electrodes are not conductive, and the detected current signal is close to zero. When the test liquid comes into contact with the second electrode, the first and second electrodes become conductive, and a sudden change in current signal is detected. The time interval between the absence of current signal and the sudden change in current signal is calculated, and this time represents the sampling time of the hydrophilic membrane. This sampling time can be accurate to 0.1s, thus allowing for precise evaluation of the sampling performance of the hydrophilic membrane.

[0032] Therefore, hydrophilicity assessment is transformed from surface physicochemical measurement to fluid dynamics performance measurement. By monitoring the time interval between the abrupt change from no current signal to current signal between the first and second electrodes of the target test paper, a quantitative assessment of the hydrophilic properties of the hydrophilic membrane material can be achieved. The operation is simple and requires no complex calculations. Furthermore, the time interval between the abrupt change from no current signal to current signal between the first and second electrodes of the target test paper reflects the dynamic flow of the test liquid in the target test paper, thus capturing the dynamic changes of the test liquid in the hydrophilic membrane. Compared with static surface measurement methods (such as the water contact angle method) in related technologies, this can improve the accuracy of hydrophilicity assessment results.

[0033] For ease of understanding, the detection method provided in this disclosure for evaluating the hydrophilic properties of hydrophilic membrane materials in electrochemical test papers will be further explained below.

[0034] For example, the insulating substrate of the target test paper has a conductive line layer and an electrode line layer printed on it. The ink used to print the conductive line layer can be conductive carbon ink, conductive silver ink, platinum, etc., and the ink used to print the electrode line layer can be conductive carbon ink, conductive silver ink, etc. After the conductive line layer and electrode line layer are printed, an insulating ink layer can be printed on it.

[0035] For example, the electrode layer includes a first electrode and a second electrode, where the first electrode is a contact electrode and the second electrode is a sample absorption electrode. In some embodiments, the preset distance between the first electrode and the second electrode of the target test strip is 1-20 mm. Preferably, the distance between the first electrode and the second electrode of the target test strip is 5-10 mm.

[0036] For example, a hydrophilic membrane can be processed according to the structural design requirements of an electrochemical test strip and bonded to a top cover with double-sided adhesive. This top cover is then attached to the electrode line surface of an insulating substrate to form a sample absorption channel. For ease of sample absorption, venting holes can be provided on the hydrophilic membrane. In some embodiments, the length of the sealed channel formed by the hydrophilic membrane bonded to the insulating substrate with double-sided adhesive is at least greater than the distance between the first and second electrodes of the target test strip.

[0037] For example, refer to Figure 2 The target test strip may include an insulating substrate 1, an electrode line layer and a conductive line layer 2, a first electrode 201, a second electrode 202, double-sided adhesive 3, a hydrophilic film 4, and an exhaust hole 401.

[0038] For example, one end of the target test strip is provided with a sample inlet channel for contacting the test liquid, used to guide and receive the test liquid and introduce it to the starting end of capillary action. This sample inlet channel can be formed by creating a notch or groove in the uppermost insulating ink layer. For example, refer to... Figure 2 This can be a natural extension and opening forward of the sample absorption channel (formed by the double-sided adhesive tape 3 cutouts). It should be understood that the sealed channel formed by the hydrophilic membrane bonded to the insulating substrate via double-sided adhesive is the sample absorption channel, the main channel through which the test liquid flows uniformly and automatically under capillary action. For example, refer to... Figure 2 It could be the gap formed after the double-sided tape 3 is cut out.

[0039] For example, a detection voltage can be applied to the first and second electrodes of the target test strip, and then the target test solution can be brought into contact with the sample injection channel of the target test strip. The time interval between the first and second electrodes of the target test strip from no current signal to the sudden change of current signal can be monitored. Finally, the hydrophilicity performance evaluation result of the hydrophilic membrane can be obtained based on the time interval.

[0040] It should be understood that before the test solution is introduced, the electrodes are dry, the resistance is close to infinity, and the current signal is a stable "baseline" close to zero. Applying the detection voltage before introducing the test solution ensures that the initial state of all tests is consistent and the timing is accurate, allowing for capture from the very beginning of the flow, thereby improving the accuracy of the hydrophilicity assessment results.

[0041] In some embodiments, the target test strip is obtained by cutting a first hydrophilic membrane sample from the same position of multiple hydrophilic membranes of the same type and processing it according to the structural design requirements of the electrochemical test strip. Accordingly, in step S103, the target test solution can be brought into contact with the sample inlet channel of the first test strip in the target test strip, and the first time interval between the first electrode and the second electrode of the first test strip from no current signal to the presence of current signal can be monitored. The target test solution is brought into contact with the sample inlet channel of the second test strip in the target test strip, and a reverse air pressure is applied at the inlet of the sample inlet channel of the second test strip. After the duration of the reverse air pressure reaches a preset duration, the application of the reverse air pressure is stopped, and the second time interval between the first electrode and the second electrode of the second test strip from no current signal to the presence of current signal can be monitored. The preset duration is less than a preset duration threshold. In step S104, the hydrophilicity anti-interference evaluation result of the hydrophilic membrane can be obtained based on the first time interval and the second time interval.

[0042] For example, the preset duration threshold can be 0.5 seconds.

[0043] For example, a sophisticated gas pressure control system can apply reverse pressure at the inlet of the sample channel of the second test strip. This gas pressure control system may include: a gas source, a pressure regulating valve and pressure sensor, a high-speed solenoid valve or piezoelectric valve, and a sealing interface. The gas source can be a small, stable compressed air or inert gas (such as nitrogen) cylinder, or a built-in miniature gas pump. The pressure regulating valve and pressure sensor are used to precisely control the pressure of the output gas, aiming to keep the pressure at a very low level, such as tens to hundreds of Pascals (Pa), to avoid damaging the cell structure of the target test strip. The high-speed solenoid valve or piezoelectric valve is used to achieve rapid opening and closing on a millisecond-level (e.g., 0.5 seconds) basis, ensuring the short-lived nature of the reverse pressure. The sealing interface can be a soft sealing head (such as a silicone sealing ring) that matches the shape of the sample channel of the target test strip. After the test liquid is added, the interface gently presses against the inlet, forming a sealed space.

[0044] It should be understood that the hydrophilicity performance evaluation in related technologies is conducted under ideal, interference-free sampling conditions. However, this disclosure, by applying reverse air pressure, tests the performance recovery ability of the hydrophilic membrane material under non-ideal conditions, thereby obtaining the hydrophilicity anti-interference evaluation results of the hydrophilic membrane.

[0045] For example, if the difference between the first time interval without applying reverse pressure and the second time interval with applying reverse pressure is small, it indicates that the hydrophilic membrane can quickly pull back the liquid after being slightly disturbed, and has good anti-interference performance; if the difference between the first time interval without applying reverse pressure and the second time interval with applying reverse pressure is large, or if the second time interval cannot be measured, it indicates that the hydrophilic membrane forms bubbles or stops flowing after being slightly disturbed, causing the flow to stop, and has poor anti-interference performance.

[0046] In some embodiments, the target test strip is obtained by cutting second hydrophilic membrane samples from different positions of the same hydrophilic membrane and processing them according to the electrochemical test strip structure design requirements. Accordingly, in step S103, the target test solution can be contacted with the sample introduction channels of multiple target test strips respectively, and the time interval between the first electrode and the second electrode of multiple target test strips from no current signal to the presence of current signal can be monitored respectively; in step S104, for each second hydrophilic membrane sample, the local hydrophilicity performance evaluation result of the second hydrophilic membrane sample can be obtained according to the time interval of the target test strip corresponding to the second hydrophilic membrane sample, and the local hydrophilicity performance evaluation results of multiple second hydrophilic membrane samples can be compared and analyzed to obtain the hydrophilicity consistency evaluation result of the hydrophilic membrane.

[0047] For example, multiple second hydrophilic membrane samples can be cut from different positions (such as left, middle, and right) of the same hydrophilic membrane and prepared into multiple target test strips according to the structural design requirements of electrochemical test strips.

[0048] For example, the local hydrophilicity performance evaluation result can be the sampling rate obtained by dividing the electrode distance by the corresponding time interval. Here, the electrode distance is the average distance between the first and second electrodes of multiple target test strips. Thus, a set of sampling rates can be obtained, and correspondingly, the hydrophilicity consistency evaluation result can be the ratio of the average value of this set of sampling rates to the standard deviation. The higher this ratio, the better the spatial uniformity of the hydrophilic membrane, i.e., the better the hydrophilicity consistency.

[0049] In some embodiments, the target test strip is obtained by cutting a third hydrophilic membrane sample from the same position of multiple hydrophilic membranes of the same type and processing it according to the structural design requirements of the electrochemical test strip. Accordingly, in step S103, multiple target test solutions with different physicochemical properties can be brought into contact with the injection channels of multiple target test strips one by one, and the time interval between the first electrode and the second electrode of multiple target test strips from no current signal to the presence of current signal is monitored, wherein the target test solution contacted by the injection channel of each target test strip is different; in step S104, for each third hydrophilic membrane sample, the local hydrophilicity performance evaluation result of the third hydrophilic membrane sample can be obtained according to the time interval of the target test strip corresponding to the third hydrophilic membrane sample, and the local hydrophilicity performance evaluation results of multiple third hydrophilic membrane samples are compared and analyzed to obtain the hydrophilic robustness evaluation result of the hydrophilic membrane.

[0050] In some embodiments, the multiple target test solutions comprise hematocrit blood samples with different hematocrit ranges. For example, blood samples with different hematocrit ranges of 15%, 30%, 42%, 55%, and 70% are prepared.

[0051] For example, the local hydrophilicity assessment result can be the sampling velocity obtained by dividing the electrode distance by the corresponding time interval. Here, the electrode distance is the average distance between the first and second electrodes of multiple target test strips. It should be understood that a robust hydrophilic membrane exhibits a sampling velocity that decreases gradually with increasing viscosity, consistent with theoretical predictions, and maintains a usable flow rate under all test solutions. A poorly robust membrane may experience a precipitous drop in flow rate or discontinuous flow (e.g., blockage) under a specific target test solution (such as high hematocrit). Therefore, by analyzing the changes in the local hydrophilicity assessment results, the hydrophilicity robustness assessment result of the hydrophilic membrane can be obtained.

[0052] In some embodiments, the target test strip includes a room-temperature test strip processed with a hydrophilic membrane according to the electrochemical test strip structure design requirements at a first temperature, and an aged test strip obtained by placing the room-temperature test strip in a second temperature for accelerated aging treatment, wherein the first temperature is lower than the second temperature. Accordingly, in step S104, the hydrophilic stability evaluation result of the hydrophilic membrane can be obtained based on the time interval corresponding to the room-temperature test strip, the time interval corresponding to the aged test strip, and the electrode distance, wherein the electrode distance is the distance between the first electrode and the second electrode in the room-temperature test strip or the aged test strip.

[0053] For example, the first temperature is room temperature, and the second temperature is high temperature (e.g., 75°C).

[0054] It should be understood that the performance of hydrophilic membranes will degrade due to material aging during storage or use. This disclosure allows for accelerated aging treatment of prepared room-temperature test paper in a high-temperature environment (e.g., at 75°C for 3-15 days), followed by repeating steps S102-S103, measuring the time interval after aging, and thus obtaining the hydrophilic stability assessment result of the hydrophilic membrane based on the time interval before and after aging. This transforms the performance degradation, which cannot be directly observed, into a precisely measurable change in physical quantity, quantitatively assessing the hydrophilic stability of the hydrophilic membrane.

[0055] In some embodiments, the hydrophilic stability assessment results of the hydrophilic membrane can be obtained directly based on the time interval before and after aging.

[0056] For example, after processing hydrophilic membranes from three different manufacturers into electrochemical test strips, the sample absorption time for testing different hematocrit blood samples is as follows:

[0057]

[0058] The test results above show that, regardless of whether it is a room temperature test strip or a test strip that has undergone accelerated aging, the sampling time for different hematocrit blood samples using hydrophilic membranes from three different manufacturers is as follows: Manufacturer 1 < Manufacturer 2 < Manufacturer 3. This indicates that Manufacturer 1 has the fastest sampling speed, followed by Manufacturer 2, and Manufacturer 3 has the slowest. Therefore, it can be assessed that the hydrophilic membrane of Manufacturer 1 has the best hydrophilicity, followed by Manufacturer 2, and Manufacturer 3 has the worst.

[0059] In some embodiments, the hydrophilic stability assessment result of the hydrophilic membrane can be obtained based on the electrode distance and the time interval before and after aging. Specifically, the electrode distance can be divided by the time interval corresponding to the room temperature test paper to obtain the first sampling speed of the hydrophilic membrane, and the electrode distance can be divided by the time interval corresponding to the aging test paper to obtain the second sampling speed of the hydrophilic membrane; the difference between the first sampling speed and the second sampling speed can be divided by the first sampling speed to obtain the hydrophilic stability assessment result of the hydrophilic membrane.

[0060] For example, the initial sampling rate of hydrophilic membrane A is V1 = 40 mm / s, and after aging, the sampling rate V2' = 36 mm / s, with a change rate of (40-36) / 40 = 0.10 (i.e., a performance degradation of 10%). The initial sampling rate of hydrophilic membrane B is V2 = 30 mm / s, and after aging, the sampling rate V2' = 25 mm / s, with a change rate of (30-25) / 30 ≈ 0.167 (i.e., a performance degradation of approximately 16.7%). The initial sampling rate of hydrophilic membrane C is V3 = 40 mm / s, and after aging, the sampling rate V3' = 32 mm / s, with a change rate of (40-32) / 40 = 0.20 (i.e., a performance degradation of 20%). Therefore, although hydrophilic membranes A and C have the same initial performance, the change rate of hydrophilic membrane C (20%) is greater than that of hydrophilic membrane A (10%), indicating that hydrophilic membrane A has better stability than hydrophilic membrane C.

[0061] It should be understood that at least two of the above-mentioned hydrophilicity detection methods can be combined according to actual needs. For example, the time interval between the abrupt change from no current signal to current signal between the first and second electrodes of the target test strip can be used to determine the hydrophilicity stability assessment result, hydrophilicity robustness assessment result, hydrophilicity consistency assessment result, and hydrophilicity anti-interference assessment result. Then, a weight can be assigned to each assessment result (for example, by backfitting a large amount of test strip performance data using expert experience or machine learning). Based on the weights and each assessment result, a comprehensive hydrophilicity performance score can be output. This allows for a more comprehensive evaluation of the hydrophilic properties of the hydrophilic membrane.

[0062] Secondly, this disclosure provides a detection system for evaluating the hydrophilic properties of hydrophilic membrane materials in electrochemical test strips. (Refer to...) Figure 3 A detection system 300 for evaluating the hydrophilic properties of a hydrophilic membrane material in an electrochemical test strip includes a processing device 301 and a target test strip 302 processed from the hydrophilic membrane to be tested according to the structural design requirements of the electrochemical test strip. The target test strip 302 includes an insulating substrate, a first electrode and a second electrode spaced at a predetermined distance on the insulating substrate, and a hydrophilic membrane covering the first and second electrodes and bonded to the insulating substrate with double-sided adhesive to form a sealed channel. One end of the target test strip 302 is provided with a sample inlet channel for contacting the test solution.

[0063] The target test strip 302 is used to contact the target test solution through the sample injection channel.

[0064] The processing device 301 is used to apply a detection voltage to the first electrode and the second electrode of the target test paper 302. After the target test liquid comes into contact with the sample injection channel of the target test paper 302, the time interval between the first electrode and the second electrode of the target test paper 302 from the change of no current signal to the change of current signal is monitored. Based on the time interval, the hydrophilic performance evaluation result of the hydrophilic membrane is obtained.

[0065] In some embodiments, the target test strip is obtained by cutting a first hydrophilic membrane sample from the same position of multiple hydrophilic membranes of the same type and processing it according to the structural design requirements of the electrochemical test strip. The first test strip in the target test strip is used to contact the target test solution through the injection channel; the processing device 301 is used to monitor the first time interval between the first and second electrodes of the first test strip from a sudden change in current signal to a current signal after the target test solution contacts the injection channel of the first test strip; the second test strip in the target test strip is used to contact the target test solution through the injection channel; the processing device 301 is used to apply reverse air pressure at the inlet of the injection channel of the second test strip after the target test solution contacts the injection channel of the second test strip, and after the duration of the reverse air pressure reaches a preset duration, stop applying the reverse air pressure, monitor the second time interval between the first and second electrodes of the second test strip from a sudden change in current signal to a current signal, and obtain the hydrophilicity anti-interference evaluation result of the hydrophilic membrane based on the first and second time intervals.

[0066] In some embodiments, the target test strip is obtained by cutting a second hydrophilic membrane sample from different positions on the same hydrophilic membrane and processing it according to the structural design requirements of the electrochemical test strip. Multiple target test strips are used to contact the target test solution through the sample introduction channel. The processing device 301 is used to monitor the time interval between the first and second electrodes of the multiple target test strips from the point of no current signal to the point of current signal change. For each second hydrophilic membrane sample, based on the time interval of the target test strip corresponding to the second hydrophilic membrane sample, the local hydrophilicity performance evaluation result of the second hydrophilic membrane sample is obtained, and the local hydrophilicity performance evaluation results of the multiple second hydrophilic membrane samples are compared and analyzed to obtain the hydrophilicity consistency evaluation result of the hydrophilic membrane.

[0067] In some embodiments, the target test strip is obtained by cutting a third hydrophilic membrane sample from the same position of multiple hydrophilic membranes of the same type and processing it according to the structural design requirements of electrochemical test strips. Multiple target test strips are used to contact multiple target test solutions with different physicochemical properties one-to-one through the sample introduction channel, wherein the target test solution contacted by the sample introduction channel of each target test strip is different; the processing device 301 is used to monitor the time interval between the first and second electrodes of the multiple target test strips from the point of no current signal to the point of current signal abruptly, and for each third hydrophilic membrane sample, based on the time interval of the target test strip corresponding to the third hydrophilic membrane sample, obtain the local hydrophilicity performance evaluation result of the third hydrophilic membrane sample, and compare and analyze the local hydrophilicity performance evaluation results of the multiple third hydrophilic membrane samples to obtain the hydrophilic robustness evaluation result of the hydrophilic membrane.

[0068] In some embodiments, the multiple target test solutions include hematocrit blood samples with different hematocrit ranges.

[0069] In some embodiments, the target test strip includes a room-temperature test strip processed with a hydrophilic membrane according to electrochemical test strip structure design requirements at a first temperature, and an aged test strip obtained by placing the room-temperature test strip at a second temperature for accelerated aging treatment, wherein the first temperature is lower than the second temperature. The processing device 301 is used to obtain a hydrophilic stability evaluation result of the hydrophilic membrane based on the time interval corresponding to the room-temperature test strip, the time interval corresponding to the aged test strip, and the electrode distance, wherein the electrode distance is the distance between the first electrode and the second electrode in the room-temperature test strip or the aged test strip.

[0070] In some embodiments, the processing device 301 is used to divide the electrode distance by the time interval corresponding to the room temperature test paper to obtain a first sampling speed of the hydrophilic membrane, and divide the electrode distance by the time interval corresponding to the aging test paper to obtain a second sampling speed of the hydrophilic membrane; and divide the difference between the first sampling speed and the second sampling speed by the first sampling speed to obtain the hydrophilic stability evaluation result of the hydrophilic membrane.

[0071] In some embodiments, the preset distance between the first electrode and the second electrode of the target test strip is 1-20 mm.

[0072] In some embodiments, the length of the sealed channel formed by the hydrophilic membrane being bonded to the insulating substrate by double-sided adhesive is at least greater than the distance between the first electrode and the second electrode of the target test paper.

[0073] Regarding the system in the above embodiments, the specific manner in which each module performs its operations has been described in detail in the embodiments of the method in the first aspect, and will not be elaborated upon here.

[0074] The aforementioned detection system transforms hydrophilicity assessment from surface physicochemical measurement to fluid dynamics performance measurement. By monitoring the time interval between the abrupt change from no current signal to current signal between the first and second electrodes of the target test paper, a quantitative assessment of the hydrophilic properties of the hydrophilic membrane material can be achieved. The operation is simple and requires no complex calculations. Furthermore, the time interval between the abrupt change from no current signal to current signal between the first and second electrodes of the target test paper reflects the dynamic flow of the test liquid within the test paper, thus capturing the dynamic changes of the test liquid within the hydrophilic membrane. Compared to static surface measurement methods (such as the water contact angle method) in related technologies, this improves the accuracy of hydrophilicity assessment results.

[0075] The preferred embodiments of this disclosure have been described in detail above with reference to the accompanying drawings. However, this disclosure is not limited to the specific details of the above embodiments. Within the scope of the technical concept of this disclosure, various simple modifications can be made to the technical solutions of this disclosure, and these simple modifications all fall within the protection scope of this disclosure.

[0076] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, this disclosure will not describe the various possible combinations separately.

[0077] Furthermore, various different embodiments of this disclosure can be combined in any way, as long as they do not violate the spirit of this disclosure, they should also be regarded as the content disclosed in this disclosure.

Claims

1. A detection method for evaluating the hydrophilic properties of hydrophilic membrane materials in electrochemical test paper, characterized in that, include: According to the structural design requirements of electrochemical test paper, the hydrophilic membrane is processed into a target test paper. The target test paper includes an insulating substrate, a first electrode and a second electrode disposed on the insulating substrate at a predetermined distance, and the hydrophilic membrane covering the first electrode and the second electrode and bonded to the insulating substrate with double-sided adhesive to form a closed channel. One end of the target test paper is provided with a sample inlet channel for contacting the test liquid. A detection voltage is applied to the first and second electrodes of the target test strip; The target test solution is brought into contact with the sample inlet channel of the target test strip, and the time interval between the abrupt change from no current signal to current signal between the first electrode and the second electrode of the target test strip is monitored. The hydrophilic properties of the hydrophilic membrane are evaluated based on the time interval. The target test strip comprises a first target test strip obtained by cutting a first hydrophilic membrane sample from the same position of multiple hydrophilic membranes of the same type and processing it according to the structural design requirements of an electrochemical test strip. The step of contacting the target test solution with the sample introduction channel of the target test strip and monitoring the time interval between the abrupt change from no current signal to a current signal between the first and second electrodes of the target test strip includes: The target test solution is brought into contact with the sample inlet channel of the first test strip in the first target test strip. The first time interval between the first electrode and the second electrode of the first test strip from the absence of current signal to the presence of current signal is monitored. The target test solution is brought into contact with the sample inlet channel of the second test strip in the first target test strip. A reverse air pressure is applied at the inlet of the sample inlet channel of the second test strip. After the duration of the reverse air pressure reaches a preset duration, the application of the reverse air pressure is stopped. The second time interval between the first electrode and the second electrode of the second test strip from the absence of current signal to the presence of current signal is monitored. The preset duration is less than a preset duration threshold. The step of obtaining the hydrophilic performance evaluation result of the hydrophilic membrane based on the time interval includes: The hydrophilicity and anti-interference evaluation results of the hydrophilic membrane are obtained based on the first time interval and the second time interval.

2. The detection method for evaluating the hydrophilic properties of hydrophilic membrane materials in electrochemical test paper according to claim 1, characterized in that, The target test strip includes a second target test strip obtained by cutting a second hydrophilic membrane sample from different positions of the same hydrophilic membrane and processing it according to the electrochemical test strip structure design requirements; The step of contacting the target test solution with the sample introduction channel of the target test strip and monitoring the time interval between the abrupt change from no current signal to a current signal between the first and second electrodes of the target test strip includes: The target test solution is brought into contact with the injection channels of multiple second target test strips, and the time interval between the first and second electrodes of multiple second target test strips from no current signal to current signal is monitored. The step of obtaining the hydrophilic performance evaluation result of the hydrophilic membrane based on the time interval includes: For each second hydrophilic membrane sample, the local hydrophilicity performance evaluation result of the second hydrophilic membrane sample is obtained according to the time interval of the second target test paper corresponding to the second hydrophilic membrane sample. The local hydrophilicity performance evaluation results of the multiple second hydrophilic membrane samples are compared and analyzed to obtain the hydrophilicity consistency evaluation result of the hydrophilic membrane.

3. The detection method for evaluating the hydrophilic properties of hydrophilic membrane materials in electrochemical test paper according to claim 1, characterized in that, The target test strip includes a third target test strip, which is obtained by cutting a third hydrophilic membrane sample from the same position of multiple hydrophilic membranes of the same type and processing it according to the structural design requirements of electrochemical test strips. The step of contacting the target test solution with the sample introduction channel of the target test strip and monitoring the time interval between the abrupt change from no current signal to a current signal between the first and second electrodes of the target test strip includes: Multiple target test solutions with different physicochemical properties are brought into contact with the injection channels of multiple third target test strips one by one, and the time interval between the first electrode and the second electrode of multiple third target test strips from no current signal to current signal is monitored. The target test solution contacted by the injection channel of each third target test strip is different. The step of obtaining the hydrophilic performance evaluation result of the hydrophilic membrane based on the time interval includes: For each third hydrophilic membrane sample, the local hydrophilicity performance evaluation result of the third hydrophilic membrane sample is obtained according to the time interval of the third target test paper corresponding to the third hydrophilic membrane sample. The local hydrophilicity performance evaluation results of multiple third hydrophilic membrane samples are compared and analyzed to obtain the hydrophilic robustness evaluation result of the hydrophilic membrane.

4. The detection method for evaluating the hydrophilic properties of hydrophilic membrane materials in electrochemical test paper according to claim 3, characterized in that, The multiple target test solutions include hematocrit blood samples with different hematocrit ranges.

5. The detection method for evaluating the hydrophilic properties of hydrophilic membrane materials in electrochemical test paper according to claim 1, characterized in that, The target test strip includes a room temperature test strip obtained by processing a hydrophilic membrane according to the electrochemical test strip structure design requirements at a first temperature, and an aged test strip obtained by placing the room temperature test strip at a second temperature for accelerated aging treatment, wherein the first temperature is lower than the second temperature; The step of obtaining the hydrophilic performance evaluation result of the hydrophilic membrane based on the time interval includes: The hydrophilic stability assessment result of the hydrophilic membrane is obtained based on the time interval corresponding to the room temperature test paper, the time interval corresponding to the aging test paper, and the electrode distance, wherein the electrode distance is the distance between the first electrode and the second electrode in the room temperature test paper or the aging test paper.

6. The detection method for evaluating the hydrophilic properties of hydrophilic membrane materials in electrochemical test paper according to claim 5, characterized in that, The step of obtaining the hydrophilic stability assessment result of the hydrophilic membrane based on the time interval corresponding to the room temperature test strip, the time interval corresponding to the aging test strip, and the electrode distance includes: Divide the electrode distance by the time interval corresponding to the room temperature test paper to obtain the first sampling speed of the hydrophilic membrane, and divide the electrode distance by the time interval corresponding to the aging test paper to obtain the second sampling speed of the hydrophilic membrane; The difference between the first sampling speed and the second sampling speed is divided by the first sampling speed to obtain the hydrophilic stability evaluation result of the hydrophilic membrane.

7. The detection method for evaluating the hydrophilic properties of hydrophilic membrane materials in electrochemical test paper according to any one of claims 1-6, characterized in that, The preset distance between the first and second electrodes of the target test strip is 1-20mm.

8. The detection method for evaluating the hydrophilic properties of hydrophilic membrane materials in electrochemical test paper according to any one of claims 1-6, characterized in that, The length of the sealed channel formed by the hydrophilic membrane bonded to the insulating substrate with double-sided adhesive is at least greater than the distance between the first electrode and the second electrode of the target test paper.

9. A detection system for evaluating the hydrophilic properties of a hydrophilic membrane material in electrochemical test paper, characterized in that, The test strip includes a processing device and a target test strip processed from a hydrophilic membrane to be tested according to the structural design requirements of an electrochemical test strip. The target test strip includes an insulating substrate, a first electrode and a second electrode disposed on the insulating substrate at a predetermined distance, and the hydrophilic membrane covering the first electrode and the second electrode and bonded to the insulating substrate with double-sided adhesive to form a closed channel. One end of the target test strip is provided with a sample inlet channel for contacting the test liquid. The target test strip is used to contact the target test liquid through the sample introduction channel; The processing device is used to apply a detection voltage to the first and second electrodes of the target test strip. After the target test liquid comes into contact with the sample inlet channel of the target test strip, it monitors the time interval between the first and second electrodes of the target test strip from no current signal to a sudden change in current signal. Based on the time interval, it obtains the hydrophilic performance evaluation result of the hydrophilic membrane. The target test strip comprises a first target test strip obtained by cutting a first hydrophilic membrane sample from the same position of multiple hydrophilic membranes of the same type and processing it according to the structural design requirements of electrochemical test strips. The processing device is used to monitor the first time interval between the first electrode and the second electrode of the first target test strip from the point of no current signal to the point of current signal after the target test liquid comes into contact with the sample inlet channel of the first target test strip. After the target test liquid comes into contact with the sample inlet channel of the second target test strip, a reverse air pressure is applied at the inlet of the sample inlet channel of the second test strip. After the duration of the reverse air pressure reaches a preset duration, the application of the reverse air pressure is stopped. The second time interval between the first electrode and the second electrode of the second test strip from the point of no current signal to the point of current signal is monitored. Based on the first time interval and the second time interval, the hydrophilicity anti-interference evaluation result of the hydrophilic membrane is obtained.

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