基于比色法的槲皮素快速检测方法

By employing an adaptive data acquisition and processing method, the problem of interference signals from complex sample matrices in quercetin colorimetric detection was solved, achieving more efficient capture and separation of quercetin characteristic signals and improving the accuracy and sensitivity of the detection.

CN122042648BActive Publication Date: 2026-07-17LINQU TIANLI BIOLOGICAL PROD CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
LINQU TIANLI BIOLOGICAL PROD CO LTD
Filing Date
2026-04-13
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing colorimetric detection methods for quercetin struggle to effectively resolve interference signals from multiple coexisting components in complex sample matrices, resulting in insufficient specificity and accuracy of the detection results. Furthermore, existing data acquisition strategies fail to adequately consider the non-uniform distribution of data quality in the time-spectral domain, limiting the improvement of detection sensitivity and resolution.

Method used

An adaptive data acquisition and processing method is adopted. By topological segmentation of spectral response surface, dynamic spectral streamline data acquisition, static spectral background data generation, and deconvolution using iterative optimization algorithms, the quercetin feature signal and interference background signal are separated, a quantitative descriptor subset is constructed and pattern matching is performed, and the quercetin concentration is calculated.

Benefits of technology

It improves the signal-to-noise ratio and information purity of the data, enhances the distinguishability of characteristic signals in the time-spectral domain, enables more reliable quantitative analysis, and improves the sensitivity and resolution of detection.

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Abstract

本发明公开了基于比色法的槲皮素快速检测方法,涉及分析化学检测技术领域,包括获取显色反应后的光谱响应曲面,依据信噪比分割为主、辅分析区域,并在主区域动态调整网格密度进行采集。通过沿吸光度梯度构建流线采集动态数据,在辅助区域进行等间距采样,融合生成复合数据集。经迭代解卷积分离出目标特征信号,提取峰参数后与校准图谱进行模式匹配,依据权重计算浓度值。该方法通过自适应区域化采样与流线追踪的动态数据获取策略,强化了对特征信号演变轨迹的捕捉,有效抑制了背景干扰,提升了复杂体系中槲皮素定量分析的准确性与可靠性。
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