A method for constructing an animal model of OPC exhaustion in the corpus callosum brain region
By specifically knocking out the Pdgfa gene in newly formed oligodendrocytes, an OPC exhaustion model of the corpus callosum brain region was constructed using Cnp-Cre mice. This solved the problem of the inability to construct stable and specific OPC exhaustion in existing technologies, and enabled the application of a highly stable OPC exhaustion model.
CN122074449APending Publication Date: 2026-05-26HANGZHOU NORMAL UNIVERSITY
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Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- HANGZHOU NORMAL UNIVERSITY
- Filing Date
- 2026-04-27
- Publication Date
- 2026-05-26
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Abstract
This invention discloses a method for constructing an animal model of OPC depletion in the corpus callosum brain region, through specific gene knockout or gene silencing in newly formed oligodendrocytes. Pdgfa Genes were used to obtain an animal model of OPC depletion in the corpus callosum brain region. This invention's animal model, through Cnp-Cre-mediated specific knockout, disrupts only NFO. Pdgfa The gene only causes OPC depletion in the white matter region of the brain and does not affect other tissues or cell types. Pdgfa This method effectively avoids interference with multiple organs throughout the body. The model mice showed a stable OPC depletion rate of ≥85% at 6 months of age, with significantly reduced OL (oligomyelination) and minimal inter-individual variability, demonstrating good experimental reproducibility. It can be applied to various fields, including research on OPC homeostasis regulation mechanisms, pathogenesis of demyelinating diseases, drug screening and treatment efficacy evaluation for diseases related to brain white matter development.
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