An anti-photo-damage composition comprising paris extract, astragalus extract and poria extract and application thereof

Abstract

The application discloses an anti-light damage composition containing Paris extract, Astragalus extract and Poria extract and application thereof. The composition contains Paris extract, Astragalus extract and Poria extract in a mass ratio of 2-9:5-32:2-4. The anti-light damage ability, skin damage repair, skin barrier ability and skin moisturizing ability of the composition are significantly improved through the synergistic effect of the combination of Paris extract, Astragalus extract and Poria extract. The composition is used for preparing cosmetics, and the quality of natural raw material cosmetics is improved.

Description

Technical Field

[0001] This application belongs to the field of cosmetic technology, specifically relating to an anti-photodamage composition comprising Paris polyphylla extract, Astragalus membranaceus extract and Poria cocos extract, and its application. Background Technology

[0002] The Chinese medicinal herb *Paris polyphylla* is the dried rhizome of *Paris polyphylla*. It has a bitter taste and slightly cold nature; it is slightly toxic. It has the effects of clearing heat and detoxifying, reducing swelling and relieving pain, cooling the liver and calming the nerves. It is commonly used for boils, carbuncles, sore throat, snake and insect bites, injuries from falls, and infantile convulsions.

[0003] Astragalus membranaceus, a traditional Chinese medicine, is derived from the root of the plant. Total flavonoids of astragalus (TFA) are one of its main active components, possessing various biological functions including antioxidant, antitumor, and antimutagenic effects. Astragalus polysaccharides (APS) are another major active component of astragalus, serving as an immune enhancer that can activate the animal immune system. APS plays an important role in antitumor, antiviral, antioxidant, blood sugar control, and cardiovascular function improvement.

[0004] Poria cocos, a traditional Chinese medicine, is the dried sclerotium of the Poria cocos plant. It has a sweet, bland, and neutral flavor, and enters the heart, spleen, and kidney meridians. It possesses functions such as promoting diuresis and eliminating dampness, strengthening the spleen and calming the mind. The main active ingredient in Poria cocos is Poria cocos polysaccharide. Poria cocos polysaccharide has anti-tumor, antiviral, antioxidant, immune-enhancing, liver-protective, hypnotic, anti-inflammatory, and litholytic effects, and can be widely used in medical and health care, food, and other fields.

[0005] With the widespread application of traditional Chinese medicine extracts in the cosmetics industry, their unique efficacy is increasingly valued. Traditional Chinese medicine compositions have significant effects on skin soothing and repair, and protection against photodamage, while exhibiting good safety and biocompatibility. For example, patent CN110025547A discloses a composition for repairing blue light damage, its preparation method, and its application. Through the synergistic effect of nasturtium / leaf / stem extract and ginger root extract, the composition significantly improves the repair effect on blue light-induced skin damage and has a certain degree of blue light resistance. Patent CN118453473A discloses a photodamage-resistant composition containing multiple plant extracts, composed of chayote fruit extract, chili fruit extract, sappanwood bark extract, and knotweed extract. The composition can resist photodamage from multiple dimensions, including scavenging free radicals and inflammatory factors, and has higher safety compared to conventional sunscreens. Patent CN118511989A discloses an edible anti-photodamage composition for the skin and its application. The composition consists of 60 parts acerola cherry concentrate powder, 30 parts amla powder, 9 parts hibiscus powder, 36 parts tea tree pollen, and 4 parts rose pollen. This anti-photodamage composition has the effects of scavenging reactive oxygen free radicals, reducing matrix metalloproteinase activity, and promoting the synthesis of elastin and type I collagen; it also has the effect of reducing the expression of inflammation-related factors IL-6 and MCP-1; and it promotes the formation of autophagosomes and the expression of autophagy-associated protein 7 (ATG7) and human microtubule-associated protein light chain 3b (LC3b), thereby regulating the autophagy of skin fibroblasts and achieving the effects of repairing the skin and delaying aging. Patent CN116158992A discloses a composition with the effect of repairing cell photodamage and its application. The composition consists of retinol and ginkgo leaf extract in a mass ratio of 1:0.5~10. The combination of retinol and ginkgo leaf extract can synergistically repair cell photodamage.

[0006] Currently, Paris polyphylla, Astragalus membranaceus, and Poria cocos are all used in cosmetics. For example, patent CN117338678A discloses the application of Paris polyphylla extract in the preparation of beauty products with anti-inflammatory and / or soothing effects on the skin; patent CN116712369A discloses a traditional Chinese medicine skincare product and its preparation method, wherein the traditional Chinese medicines include 0.001-1% Astragalus membranaceus extract, 0.001-6% Bletilla striata extract, and 0.001-6% Coix lacryma-jobi extract. Traditional Chinese medicine skincare products can whiten, provide antioxidant effects, moisturize, and prevent collagen loss. Patent CN107669525B discloses the application and preparation method of Poria cocos extract with brightening effects. The Poria cocos extract acts on keratinocytes, improving skin roughness and radiance, and can be widely used in the cosmetics industry.

[0007] However, there are no reports of cosmetics containing all three of the above-mentioned traditional Chinese medicines. Therefore, it is of great significance to develop a cosmetic containing Paris polyphylla, Astragalus membranaceus, and Poria cocos to give full play to the synergistic effect of the three traditional Chinese medicines and meet people's increasingly higher consumer demand for cosmetic quality. Summary of the Invention

[0008] In order to fully explore the efficacy of Paris polyphylla, Astragalus membranaceus, and Poria cocos, improve the skin's anti-photodamage repair function of cosmetics, and meet people's multifunctional consumption needs for cosmetics, this invention provides the following technical solution.

[0009] In a first aspect, the present invention provides a composition comprising Paris polyphylla extract, Astragalus membranaceus extract and Poria cocos extract.

[0010] Preferably, the mass ratio of the Paris polyphylla extract, Poria cocos extract, and Astragalus membranaceus extract is 2~9:5~32:2~4.

[0011] Preferably, the Paris polyphylla extract is obtained from one or more of the following Paris polyphylla species: Yunnan Paris polyphylla, Paris polyphylla var. yunnanensis ...

[0012] Preferably, the Paris polyphylla extract comprises a first Paris polyphylla extract and a second Paris polyphylla extract, wherein the first Paris polyphylla extract and the second Paris polyphylla extract are made from different types of raw materials.

[0013] The raw materials for the first Paris polyphylla extract are Paris polyphylla yunnanensis, Paris polyphylla var. chinensis, Paris polyphylla var. ovoidensis, Paris polyphylla daliensis, Paris polyphylla var. polyphylla, Paris polyphylla var. longissimus, Paris polyphylla var. globosa, Paris polyphylla var. shortissimus, Paris polyphylla var. blackis, Paris polyphylla var. five-finger or Paris polyphylla var. longissimus.

[0014] The raw materials for the second Paris polyphylla extract are Paris polyphylla yunnanensis, Paris polyphylla var. chinensis, Paris polyphylla var. ovoidensis, Paris polyphylla daliensis, Paris polyphylla var. polyphylla, Paris polyphylla var. longissimus, Paris polyphylla var. globosa, Paris polyphylla var. shortissimus, Paris polyphylla var. blackis, Paris polyphylla var. five-finger or Paris polyphylla var. longissimus.

[0015] Preferably, the mass ratio of the first Paris polyphylla extract, the second Paris polyphylla extract, the Astragalus membranaceus extract, and the Poria cocos extract is 1~5:1~4:5~32:2~4, for example: 1:1:20:3, 1:4:32:2, 2:1:5:2, 3:2:8:3, 4:3:10:4, 5:2:15:4, 5:4:30:3.

[0016] Preferably, the preparation process of the Paris polyphylla extract includes the following steps: (1) The dried Paris polyphylla product was coarsely pulverized and extracted by reflux to obtain an extract; (2) The extract from step (1) is filtered and concentrated to obtain a concentrated solution; (3) The concentrate from step (2) is purified by elution to obtain the eluent; (4) The eluent from step (3) is concentrated and dried to obtain the Paris polyphylla extract.

[0017] Preferably, in step (1), the coarse powder particles should meet the following conditions: 95% can pass through a No. 1 pharmacopoeia sieve with a mesh size of 10 and a particle size of 2 mm, and all particles have a diameter of less than 3 mm; and no more than 20% of the powder can pass through a No. 4 pharmacopoeia sieve with a mesh size of 65 and a particle size of 0.25 mm.

[0018] Preferably, in step (1), the reflux extraction method is atmospheric pressure reflux extraction.

[0019] Furthermore, the extraction time is 1~5 hours / time, preferably 1~4 hours / time, and more preferably 2~3 hours / time.

[0020] Furthermore, the extraction is performed 1 to 6 times, preferably 1 to 5 times, and more preferably 2 to 3 times.

[0021] Furthermore, the extraction solvent is ethanol or water, preferably ethanol, and more preferably 50-75% ethanol.

[0022] Furthermore, the amount of solvent used is 1 to 50 times the dry weight of the *Paris polyphylla* sphere, preferably 10 to 30 times, and most preferably 15 to 20 times.

[0023] Preferably, in step (2), the filter screen used for filtration is 150-300 mesh, more preferably 150-250 mesh, and most preferably 200 mesh.

[0024] Preferably, in step (2), the concentration method is low-temperature vacuum concentration, and more preferably low-temperature constant-temperature high-vacuum vacuum concentration.

[0025] Furthermore, the endpoint of the concentration is that the ratio of the mass of the feed to the volume of the concentrate (W / V) is 1:0.1~2 kg / L, preferably 1:0.3~1.5 kg / L, and more preferably 1:0.4~1 kg / L.

[0026] Preferably, in step (3), the purification is carried out by macroporous adsorption resin.

[0027] Furthermore, the macroporous adsorption resin is selected from one or more of the following: D101 type macroporous adsorption resin, D101-I type macroporous adsorption resin, D4020 type macroporous adsorption resin, HPD-100 type macroporous adsorption resin, AB-8 type macroporous adsorption resin, preferably D101 type macroporous adsorption resin, HPD-100 type macroporous adsorption resin, AB-8 type macroporous adsorption resin, and most preferably D101 type macroporous adsorption resin.

[0028] Furthermore, the mass of the macroporous adsorption resin is 10 to 50 times the mass of the concentrated liquid solids, preferably 10 to 40 times, and more preferably 15 to 30 times.

[0029] Furthermore, the solution used for pretreatment of the macroporous adsorption resin is an aqueous ethanol solution, preferably a 95% aqueous ethanol solution.

[0030] Preferably, the macroporous adsorption resin is pretreated and then equilibrated with purified water for later use.

[0031] Preferably, in step (3), the purification is performed 1 to 15 times, more preferably 1 to 10 times.

[0032] Preferably, step (3) further includes the following step: (a) The purification step also includes sample loading; (b) After loading the sample, the macroporous adsorption resin column was eluted sequentially with purified water and 10%~95% ethanol aqueous solution. (c) Collect the saponin eluent; Preferably, the flow rate of the sample loading in step (a) is 0.2~5 BV / h, more preferably 0.2~3 BV / h, and even more preferably 0.5~1.5 BV / h.

[0033] Preferably, the elution flow rate in step (b) is 0.5~5 BV / h, more preferably 0.5~4 BV / h, and even more preferably 1~3 BV / h.

[0034] Preferably, in step (4), the method for concentrating the eluent is low-temperature vacuum concentration, preferably low-temperature constant-temperature high-vacuum vacuum concentration.

[0035] Preferably, in step (4), the concentration endpoint is to concentrate to a density of 1.0~1.3 mg / mL, more preferably 1.0~1.1 mg / mL.

[0036] Preferably, in step (4), the drying method is selected from one or more of the following: vacuum freeze drying, reduced pressure drying, spray drying, preferably vacuum freeze drying or reduced pressure drying.

[0037] Preferably, the preparation process of the Poria cocos extract includes the following steps: (1) Soak Poria cocos powder in water and reflux to extract the extract; (2) The extract is filtered and concentrated to obtain a concentrated solution; (3) The concentrated solution is adsorbed with macroporous resin and eluted to obtain an eluent. (4) The eluent is concentrated and dried to obtain the Poria cocos extract.

[0038] Preferably, the soaking temperature in step (1) is 75~85℃, for example: 75℃, 78℃, 80℃, 83℃, 85℃.

[0039] Preferably, the reflux extraction temperature in step (1) is 85~95℃, for example: 85℃, 88℃, 90℃, 93℃, 95℃.

[0040] Preferably, the number of reflux extractions in step (1) is 2 to 5 times, for example: 2 times, 3 times, 4 times, 5 times.

[0041] Preferably, the reflux extraction time in step (1) is 1~3 h / time, for example: 1 h / time, 1.5 h / time, 2 h / time, 2.5 h / time, 3 h / time.

[0042] Preferably, the filter mesh size in step (2) is ≥200.

[0043] Preferably, in step (3), the macroporous adsorption resin is selected from one or more of the following: D101 type macroporous adsorption resin, D101-Ⅰ type macroporous adsorption resin, D4020 type macroporous adsorption resin, HPD-100 type macroporous adsorption resin, AB-8 type macroporous adsorption resin, preferably D101 type macroporous adsorption resin, HPD-100 type macroporous adsorption resin, AB-8 type macroporous adsorption resin, and most preferably D101 type macroporous adsorption resin.

[0044] Preferably, the elution flow rate in step (3) is 0.5~5 BV / h, more preferably 0.5~4 BV / h, and even more preferably 1~3 BV / h.

[0045] Preferably, in step (4), the method for concentrating the eluent is low-temperature vacuum concentration, preferably low-temperature constant-temperature high-vacuum vacuum concentration.

[0046] Preferably, in step (4), the drying method is selected from one or more of the following: vacuum freeze drying, reduced pressure drying, spray drying, preferably vacuum freeze drying or reduced pressure drying.

[0047] Preferably, the preparation process of the Astragalus extract includes the following steps: (1) Soak Astragalus powder in ammonia water and reflux to extract the extract; (2) The extract is concentrated into an extract to obtain the Astragalus extract.

[0048] Preferably, the astragalus powder in step (1) has a mesh size ≥10, for example: 40, 60, 80, 100.

[0049] Preferably, the ammonia concentration in step (1) is 5~10 wt%, for example: 5%, 6%, 7%, 8%, 9%, 10%.

[0050] Preferably, the soaking time in step (1) is 15~30 h, for example: 15 h, 18 h, 20 h, 25 h, 28 h, 30 h.

[0051] Preferably, the reflux extraction time in step (1) is 3 to 6 hours, for example: 3 hours, 4 hours, 5 hours, or 6 hours.

[0052] Preferably, the number of reflux extractions in step (1) is 2 to 4 times, for example: 2 times, 3 times, or 4 times.

[0053] Preferably, the mass ratio of extract to extract in step (2) is 1:1 to 1.5, for example: 1:1, 1:1.01, 1:1.2, 1:1.3, 1:1.4, 1:1.5.

[0054] Preferably, the concentration temperature in step (2) is 30~40℃, for example: 30℃, 32℃, 35℃, 37℃, 40℃.

[0055] In a second aspect, the present invention provides a cosmetic product comprising the composition according to the first aspect.

[0056] Preferably, the cosmetic also includes excipients that are permitted to be added to the cosmetic.

[0057] Preferably, the cosmetic is a leave-on cosmetic, such as: toner, serum, lotion, cream, face cream, gel or jelly.

[0058] Furthermore, the formula of the serum is shown in the table below: Furthermore, the formula of the face cream is shown in the table below: Thirdly, the present invention provides the use of the composition described in the second aspect, the use comprising at least one of the following: (1) Application in the preparation of products that help improve skin hydration; (2) Application in the preparation of products that help improve photodamage to the skin; (3) Application in the preparation of products that help promote the repair of photodamage to the skin.

[0059] Preferably, the product includes pharmaceuticals or cosmetics.

[0060] Furthermore, the product also includes excipients permitted for use in pharmaceuticals or cosmetics.

[0061] Preferably, the excipients include, but are not limited to: cellulose and its derivatives, gelatin, talc, solid lubricants, calcium sulfate, vegetable oil, polyols, emulsifiers, wetting agents, buffers, chelating agents, thickeners, pH adjusters, transdermal penetration enhancers, colorants, flavoring agents, stabilizers, preservatives, antibacterial agents, or pyrogen-free water.

[0062] Furthermore, the cellulose derivative includes methylcellulose, ethylcellulose, hydroxypropyl methylcellulose, or sodium carboxymethylcellulose.

[0063] Furthermore, the solid lubricant includes stearic acid or magnesium stearate.

[0064] Furthermore, the vegetable oil includes soybean oil, sesame oil, or olive oil.

[0065] Furthermore, the polyol includes propylene glycol, glycerol, or sorbitol.

[0066] Preferably, the dosage form of the drug or cosmetic is a solid dosage form, a semi-solid dosage form, or a liquid dosage form.

[0067] Preferably, the dosage form of the drug is an oral preparation.

[0068] Preferably, the dosage form of the drug or cosmetic is a topical preparation.

[0069] Preferably, the dosage form of the drug or cosmetic is a cream, lotion, solution, spray, or patch.

[0070] The beneficial effects of this invention are: This invention combines Paris polyphylla extract, Astragalus membranaceus extract, and Poria cocos extract, and through the synergistic effect between the components, it significantly enhances the composition's ability to resist photodamage, repair skin damage, improve skin barrier function, and moisturize the skin. When this composition is used to prepare cosmetics, it can enrich the functions of cosmetics, improve the quality of cosmetics, and meet the different consumer needs of people. Attached Figure Description

[0071] Figure 1The cell viability of the composition and single raw material of the present invention in a UVB-induced photodamage cell model; This indicates that compared to the UVB model group, P < 0.05; This indicates that compared to the UVB model group, P < 0.01; # indicates P < 0.001 compared to the UVB model group; # indicates P < 0.05 compared to composition 1; ## indicates P < 0.01 compared to composition 1; ### indicates P < 0.001 compared to composition 1; △ indicates P < 0.05 compared to composition 2; △△ indicates P < 0.01 compared to composition 2; ▲ indicates P < 0.05 compared to composition 3; ▲▲ indicates P < 0.01 compared to composition 3; ▲▲▲ indicates P < 0.001 compared to composition 3.

[0072] Figure 2 The ROS fluorescence intensity of the composition and single raw material of the present invention in a UVB-induced photodamage cell model; This indicates that compared to the UVB model group, P < 0.05; This indicates that compared to the UVB model group, P < 0.01; # indicates P < 0.001 compared to the UVB model group; # indicates P < 0.05 compared to composition 1; ## indicates P < 0.01 compared to composition 1; ### indicates P < 0.001 compared to composition 1; △ indicates P < 0.05 compared to composition 2; △△ indicates P < 0.01 compared to composition 2; △△△ indicates P < 0.001 compared to composition 3; ▲ indicates P < 0.05 compared to composition 3.

[0073] Figure 3 The effect of UVB-induced photodamage on the skin after applying the skin care essence cosmetic of Example 7 of the present invention.

[0074] Figure 4 The total score of the skin care essence in Example 7 of this invention on UVB-induced skin damage.

[0075] Figure 5 The skin care essence of Example 7 of this invention is used to score the erythema caused by UVB-induced skin damage.

[0076] Figure 6 This invention relates to the effect of skincare essence in Example 7 on the moisture content (CM) value of the stratum corneum in UVB-induced photodamage. This indicates that P < 0.001 compared to the baseline value.

[0077] Figure 7 The effect of the skin care essence in Example 7 of the present invention on the transepidermal water loss (TEWL) value of UVB-induced photodamaged skin; This indicates that P < 0.001 compared to the baseline value.

[0078] Figure 8 Example 8 of the present invention illustrates the effect of a skincare cream on the CM value of moisture content in the stratum corneum of skin damaged by UVB-induced photodamage. This indicates that P < 0.001 compared to the baseline value.

[0079] Figure 9 The effect of the skin care cream in Example 8 of the present invention on the transepidermal water loss (TEWL) value of UVB-induced photodamaged skin; This indicates that P < 0.001 compared to the baseline value. Detailed Implementation

[0080] The technical solution of the present invention will be clearly and completely described below with reference to the embodiments. The described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0081] It should be noted that the terms "first" and "second" in the abstract, claims and specification of this application are used only to distinguish similar objects and should not be construed as a specific order.

[0082] In this article, the Astragalus extract is an extract from the dried root of Astragalus membranaceus (Fisch.) Bge.

[0083] In this article, the extract of Paris polyphylla is the extract of the dried rhizomes of Paris polyphylla yunnanensis, Paris polyphylla var. chinensis, Paris polyphylla var. septum ...

[0084] In this article, the Poria cocos extract is the extract of the dried sclerotium of Poria cocos (Schw.) Wolf.

[0085] The materials used in the following embodiments are as follows: Astragalus, Poria, and Paris polyphylla were purchased from Yunnan Yicao Yiwei Traditional Chinese Medicine Industry Co., Ltd. DMEM high-glucose culture medium, phosphate-buffered saline (PBS), fetal bovine serum (FBS), and penicillin-streptomycin antibiotics were purchased from Thermo Fisher Scientific. DMSO was purchased from Sinopharm Chemical Reagent Co., Ltd., and the reactive oxygen species detection kit was purchased from Beyotime Biotechnology Co., Ltd.

[0086] Example 1: Preparation of Paris polyphylla extract The first extract of Paris polyphylla was prepared using Paris polyphylla spheroides, and the second extract was prepared using Paris polyphylla yunnanensis. The extracts were prepared according to the following method: (1) 28.0 kg of dried Paris polyphylla was crushed using a coarse crusher (model CSJ-60) with a 3 mm mesh. 25.0 kg of the coarse Paris polyphylla powder was weighed and put into a 300 L multi-functional extraction tank (model TQ-SH). 250 L of 50% ethanol was added and soaked for 30 minutes. The mixture was then refluxed and extracted twice, each time for 2 hours. The extracts were then combined.

[0087] (2) Filter the extract from step (1) through a 200-mesh filter, and concentrate the filtrate to 23 L under reduced pressure using a 230 L single-effect external circulation evaporator (model WZ) to obtain a concentrated solution.

[0088] (3) After cooling the concentrate from step (2), add it to a 90 L macroporous adsorption resin (model D101) that has been treated with 95% ethanol aqueous solution and then equilibrated with pure water. The loading flow rate is 1 BV / h. Elute sequentially with pure water, 20%, 40%, 60%, 80%, and 95% ethanol aqueous solution, each for 2 BV, at a flow rate of 2 BV / h. Collect the saponin eluent.

[0089] (4) The eluent from step (3) was concentrated under reduced pressure and then vacuum dried (model YZG-SH) to obtain Paris polyphylla extract.

[0090] Example 2: Preparation of Poria cocos extract The preparation method of Poria cocos extract is as follows: (1) Soak 1 kg of Poria cocos powder in 10 L of 80℃ water for 2 h, then heat to 90℃ for reflux extraction 3 times, 2 h each time. Add 10 L of water to the last two reflux extractions and combine to obtain the extract. (2) Filter the extract obtained in step (1) through a 200-mesh filter and concentrate it to obtain a concentrated solution; (3) Load the concentrated solution obtained in step (2) onto an 80 L macroporous adsorption resin (model D101), and elute sequentially with pure water, 30% (v / v), 50% (v / v), 65% (v / v), 80% (v / v), and 95% (v / v) ethanol aqueous solution, each eluting 4 BV at a flow rate of 2 BV / h, and collect the eluent; (4) Concentrate and dry the eluent obtained in step (3) to obtain Poria cocos extract.

[0091] Example 3: Preparation of Astragalus Extract The preparation method of Astragalus membranaceus extract is as follows: (1) Weigh 40 kg of Astragalus membranaceus coarse powder after passing through a 10-mesh sieve, soak it in 10 L of 8wt% ammonia water at room temperature (25℃) for 20 h; reflux extract for 4 h, extract 3 times, add 15 L of 8wt% ammonia water for the last two reflux extracts, and combine to obtain the extract; (2) Concentrate the extract obtained in step (1) into a paste: the mass ratio of the extract is 1:1.01, the temperature is 30℃, and the paste of Astragalus extract is obtained. Dry it to obtain Astragalus extract.

[0092] Example 4: Preparation of cosmetic composition 1 with the function of promoting skin photodamage repair According to the mass ratio of 5:2:15:4, extracts of Paris polyphylla, Paris yunnanensis, Astragalus membranaceus, and Poria cocos were weighed separately, mixed and dissolved in water to obtain a 20wt% aqueous solution, which is cosmetic composition 1.

[0093] Example 5: Preparation of cosmetic composition 2 with the function of promoting skin photodamage repair According to the mass ratio of 2:1:5:2, the extracts of Paris polyphylla, Paris yunnanensis, Astragalus membranaceus, and Poria cocos were weighed separately, mixed and dissolved in water to obtain a 20wt% aqueous solution, which is cosmetic composition 2.

[0094] Example 6: Preparation of cosmetic composition 3 with the function of promoting skin photodamage repair According to the mass ratio of 1:4:32:2, the extracts of Paris polyphylla, Paris yunnanensis, Astragalus membranaceus, and Poria cocos were weighed separately, mixed and dissolved in water to obtain a 20wt% aqueous solution, which is cosmetic composition 3.

[0095] Performance Test Example 1: Effects of cosmetic compositions 1-3 on UVB-induced proliferation of skin fibroblasts 1. Cell culture and cell plating: HFF-1 cells (human skin fibroblasts) were cultured in DMEM medium (DMEM basal medium + 10% FBS + 1% lignin solution) at 5% humidity in a 37°C incubator, with a cell density of 80%–90%. HFF-1 cells in the logarithmic growth phase were then cultured at a density of 2 × 10⁶ cells / cells. 5 Cell suspension was seeded into 96-well cell culture plates, with 100 μL of cell suspension added to each well.

[0096] 2. UVB model establishment and MTT assay for cell viability: (1) Group treatment: control group, UVB model group, Paris polyphylla extract group, Yunnan Paris polyphylla extract group, Poria cocos extract group, Astragalus membranaceus extract group, composition 1 group (cosmetic composition 1), composition 2 group (cosmetic composition 2) and composition 3 group (cosmetic composition 3) were set up. The samples were dissolved in DMSO to 200 mg / mL to prepare stock solutions for later use. When used, they were diluted with DMEM high glucose medium to the working solution concentration: the final concentrations were 0.78 ppm, 0.56 ppm, 3.13 ppm and 4 ppm, respectively.

[0097] (2) Establishment of UVB model and drug administration: When the cells adhered and grew to 80%, except for the control group, the culture medium was removed from the other groups, and the cells were washed twice with PBS. After adding 50 μL of PBS to each well, the 96-well plate was placed in a UV crosslinker for UVB irradiation (the radiation dose was 40 mJ / cm²). 2 A photodamage model was established. After the ultraviolet radiation treatment, the PBS was discarded, and 100 μL of the sample-containing culture medium was added to each well. The cells were cultured for another 24 h, and cell viability was detected by the MTT assay.

[0098] (3) MTT assay for cell viability: After incubation, discard the sample solution, wash once with PBS, add 0.5 mg / mL MTT solution, incubate in the dark for 4 h, discard the MTT solution, add 100 µL DMSO to each well, and measure the absorbance (OD) at 570 nm. Calculate the cell viability in the sample based on the absorbance.

[0099] (4) Method for calculating cell viability: Cell viability = like Figure 1 As shown, the cell survival rate of the UVB model group was (52.07±34.00)%, indicating that the UVB-induced photodamage cell model was successfully constructed. Compared with the UVB model group, the cell survival rate of composition 1 group was significantly improved in the concentration range of 0.78~4 ppm, indicating that composition 1 significantly promoted the proliferation of UVB-induced photodamage HFF-1 cells. The cell proliferation effect was highly significant in the range of 0.78~3.13 ppm (p<0.01), and the proliferation effect was most significant at a concentration of 0.78 ppm (p<0.001), with a cell survival rate of (64.09±3.89)%. The cell survival rate of composition 1 group was significantly higher than that of the single raw material group. Compositions 2 and 3 had significant proliferation effects on HFF-1 at 0.78 ppm (p<0.05).

[0100] The above results indicate that the combined use of Paris polyphylla extract, Paris yunnanensis extract, Astragalus membranaceus extract and Poria cocos extract in composition 1 at a mass ratio of 5:2:15:4 has a synergistic effect in resisting photodamage, thereby significantly improving the cell survival rate of fibroblasts after photodamage.

[0101] Performance Test Example 2: Effects of Cosmetic Compositions 1-3 on UVB-Induced Photodamage and Oxidative Stress in Fibroblasts 1. HFF-1 cell culture and cell plating: Same as performance test example 1.

[0102] 2. UVB model establishment and intracellular ROS content detection (1) Grouping treatment: A positive control group, UVB model group, Paris polyphylla extract group, Yunnan Paris polyphylla extract group, Poria cocos extract group, Astragalus membranaceus extract group, Composition 1 (cosmetic composition 1), Composition 2 (cosmetic composition 2) and Composition 3 (cosmetic composition 3) were set up. The samples were dissolved in DMSO to 200 mg / mL to prepare stock solutions for later use. When used, they were diluted with DMEM high glucose medium to the working solution concentration: the final concentrations were 0.78 ppm, 0.56 ppm, 3.13 ppm and 4 ppm, respectively.

[0103] (2) Establishment of UVB model and administration: UVB modeling is the same as in performance test case 1. Administration treatment: 50 mg / mL Rosup (S0033S, Beyotime) was added to the positive control group, and the other groups were treated the same as in performance test case 1.

[0104] (3) Detection of intracellular ROS content under UVB model: After UVB irradiation, samples were added and cultured for 24 h. The culture medium was discarded, and 100 μL of 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) diluted 1:1000 with serum-free culture medium was added to each well. The cells were incubated at 37℃ for 20 min, washed with PBS 3 times, and the fluorescence intensity was detected by an ELISA reader to determine the ROS level after cell collection.

[0105] The RFU (Relative Fluorescence Unit) value is directly positively correlated with the intracellular ROS content: the lower the RFU value, the less ROS is produced in the cell and the lower the level of oxidative stress.

[0106] like Figure 2As shown, the highest RFU value for the positive control rosup was 83,949, while the RFU value for the UVB model group was 48,576, significantly lower than that of the control group, indicating that the UVB-induced photodamage cell model was successfully constructed. Under the UVB photodamage model, composition 1 reduced ROS fluorescence intensity at both 0.78 and 4 ppm concentrations, and at 0.78 ppm, it significantly (p<0.001) reduced ROS fluorescence intensity in photodamaged HFF-1 cells, and at 4 ppm, it significantly (p<0.05) reduced ROS fluorescence intensity in photodamaged HFF-1 cells. Composition 2 significantly (p<0.05) reduced ROS fluorescence intensity in photodamaged HFF-1 cells at 0.78 ppm. Composition 3 did not significantly reduce ROS fluorescence intensity in photodamaged HFF-1 cells. Among the individual raw materials, only the extract of *Paris polyphylla* showed a significant (p<0.05) reduction in ROS levels at 0.78 ppm.

[0107] The above results indicate that the combined use of Paris polyphylla extract, Paris yunnanensis extract, Astragalus membranaceus extract and Poria cocos extract in composition 1 at a mass ratio of 5:2:15:4 has a synergistic effect in resisting photodamage and oxidative stress, thereby significantly repairing fibroblast skin cells after photodamage.

[0108] Example 7: Preparation of Skin Care Essence Prepare the serum according to the formula in Table 1. The specific steps are as follows: 1. Weigh out the raw materials of component A in Table 1, add them to the main pot, and mix and stir evenly; 2. Weigh the raw material of component B, heat it to 70℃, mix and homogenize it beforehand, and then cool it down to below 45℃; 3. Add component B to the mixing bowl and mix thoroughly; 4. Weigh out the raw materials of component C and component D, mix them separately beforehand, and then add them into the main pot in sequence and mix them evenly. 5. Filtration, discharge, aging (semi-finished product); sampling and testing, material transfer; 6. Fill and package to obtain the finished product.

[0109] Table 1 Skin Care Serum Formula Table Note: The percentages mentioned in the table are mass ratios relative to the total mass of the serum.

[0110] Example 8: Preparation of Skin Care Cream Prepare the face cream according to the formula in Table 2. The specific steps are as follows: 1. Weigh out the raw materials of component E in Table 2, add them to the main pot, and mix and stir evenly; 2. Weigh the raw material of component F, heat it to 70~80℃, add it to the main pot, mix and homogenize it evenly, and then cool it down to below 45℃; 3. Weigh out the raw materials of component G and component H, mix them separately beforehand, and then add them into the main pot in sequence and mix them evenly. 4. Filtration, discharge, aging (semi-finished product); sampling and testing, material transfer; 5. Fill and package to obtain the finished product.

[0111] Table 2 Skin Cream Formula Table Note: The percentages mentioned in the table are mass ratios relative to the total mass of the face cream.

[0112] Performance Test Example 3: Effect of Skin Care Serum on Improving UVB-Induced Photodamage Establishment and scoring of a UVB-induced acute photodamage model in mice: 1. Young female C57BL / 6J mice (5-6 weeks old, average weight 20 g) were assigned to the following 3 groups (n = 8 in each group): blank group, model group, and sample group.

[0113] 2. Skin preparation of the mouse backs: Except for the control group, the back skin of mice in other groups was exposed to UVB irradiation (200 mJ / cm²) on day 0. 2 Each group of mice was given a normal diet and water. From day 3 to day 8, 0.1 g of the skin care essence of Example 7 was applied to the back area of ​​the mice in the sample group twice a day at 12-hour intervals.

[0114] 3. Daily photos were taken to record the skin condition and a skin lesion score was calculated. Based on the researchers' experience, successful modeling was defined as the model group exhibiting obvious skin lesions such as erythema, edema, and ulceration compared to the control group. The total skin lesion score was the sum of the scores for erythema, edema, and erosion. The scoring criteria are shown in Table 3. Results are shown in [Table 3]. Figures 3-5 .

[0115] Table 3. Scoring Rules for Photodamage Skin Lesions Reference for scoring rules for photodamage skin lesions: Zhang L, Gu W, Liu T, et al. NDRG2 Deficiency Exacerbates UVB-Induced Skin Inflammation and Oxidative Stress Damage. Inflammation. 2025;48(3):1313-1325. doi:10.1007 / s10753-024-02121-3.

[0116] The test results showed that after applying a skin care essence containing a combination of Paris polyphylla, Poria cocos, and Astragalus membranaceus to the mice in the sample group, UVB-induced skin damage was significantly improved. Figure 3 The total score of photodamage lesions in the sample group was lower than that in the model group. Figure 4 Regarding the erythema index, the improvement level of the sample group was significantly better than that of the model group (). Figure 5 ).

[0117] Performance Test Example 4: Repairing Effect of Skincare Serum on UVB-Induced Photodamage Establishment and detection of a UVB-induced acute photodamage model in mice: 1. Young female C57BL / 6J mice (5-6 weeks old, average weight 20 g) were assigned to the following 3 groups (n = 8 in each group): blank group, model group, and sample group.

[0118] 2. The backs of mice were prepared with a 2 cm × 2 cm skin. Except for the control group, the back skin of mice in other groups was exposed to UVB irradiation (200 mJ / cm²) on day 0. 2 ).

[0119] 3. Each group of mice was given a normal diet and water. From day 3 to day 8, 0.1 g of the skin care essence of Example 7 was applied to the back area of ​​the mice in the sample group twice a day at 12-hour intervals.

[0120] 4. Skin tests were performed on the back skin of each group of mice before and after use (day 9): Mice were placed in a stabilization room at 25℃ and 50% humidity for 1 hour to stabilize their skin condition. After the mice's skin condition stabilized, they were anesthetized and fixed on the test table. The TEWL value was measured in the hairless area on the back of the mice using a skin moisture loss meter probe; the skin moisture content was measured in the hairless area on the back of the mice using a stratum corneum moisture content meter probe.

[0121] The test results showed that, compared with before use, the TEWL value of mice significantly decreased after 7 days of continuous use of the skin care essence containing the composition (day 9). Figure 6 ), skin moisture content increased significantly ( Figure 7 ).

[0122] Performance Test Example 5: Skin Cream Containing the Composition's Effect on Repairing UVB-Induced Photodamage The establishment and detection methods of the UVB-induced acute photodamage model in mice were the same as in performance test example 4. From day 3 to 8, 0.05 g / (time•mouse) of the skin care cream from example 8 was applied to the back area of ​​the mice in the sample group twice daily at 12-hour intervals. Skin test indicators included TEWL and skin moisture content, and the specific procedures were the same as in performance test example 4.

[0123] The test results showed that, compared with before use, the TEWL value of mice significantly decreased after continuous use of the skin care cream containing the composition for 7 days (day 9). Figure 8 ), skin moisture content increased significantly ( Figure 9 ).

[0124] The above description is merely an embodiment of the present invention and does not limit the patent scope of the present invention. Any equivalent structural transformations made based on the content of the present invention specification, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of the present invention.

Claims

1. A composition for resisting photodamage, characterized in that, The composition comprises Paris polyphylla extract, Astragalus membranaceus extract and Poria cocos extract; The Paris extract comprises a first Paris extract and a second Paris extract, wherein the first Paris extract is selected from Paris polyphylla and the second Paris extract is selected from Paris yunnanensis. The mass ratio of the first Paris polyphylla extract, the second Paris polyphylla extract, the Astragalus membranaceus extract, and the Poria cocos extract is 5:2:15:4; The preparation process of the Paris polyphylla extract includes the following steps: (1) The dried Paris polyphylla product was coarsely pulverized and extracted by reflux with 50% ethanol to obtain the extract; (2) The extract from step (1) is filtered and concentrated to obtain a concentrated solution; (3) Cool the concentrate from step (2), treat it with an ethanol aqueous solution, and then elute the macroporous adsorption resin with pure water, 20%, 40%, 60%, 80%, and 95% ethanol aqueous solution in sequence, and collect the saponin eluent. (4) The eluent from step (3) is concentrated and dried to obtain the Paris polyphylla extract; The preparation process of the Poria cocos extract includes the following steps: (1) Soak Poria cocos powder in water and reflux to extract the extract; (2) The extract is filtered and concentrated to obtain a concentrated solution; (3) The concentrate is adsorbed with macroporous resin and then eluted sequentially with pure water, 30% (v / v), 50% (v / v), 65% (v / v), 80% (v / v), and 95% (v / v) ethanol aqueous solution, and the eluent is collected. (4) The eluent is concentrated and dried to obtain the Poria cocos extract; The preparation process of the Astragalus extract includes the following steps: (1) Soak Astragalus powder in 8wt% ammonia water and reflux to extract the extract; (2) The extract is concentrated into an extract to obtain the Astragalus extract.

2. A cosmetic product, characterized in that, The cosmetic product comprises the composition of claim 1.

3. The cosmetic product according to claim 2, characterized in that, The cosmetics mentioned are toners, serums, lotions, creams, or gels.

4. The application of the composition according to claim 1, characterized in that, The application includes at least one of the following: (1) Application in the preparation of products that help improve skin hydration; (2) Application in the preparation of products that help resist photodamage.

5. The application according to claim 4, characterized in that, The products include pharmaceuticals or cosmetics.

6. The application according to claim 4, characterized in that, The product also includes excipients that are permitted to be added to pharmaceuticals or cosmetics.

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