A probiotic agent containing rhamnosus lactis cl-03 for relieving eczema, and a preparation method and application thereof

Abstract

The application belongs to the technical field of microorganisms and relates to a probiotic agent containing Bifidobacterium longum subsp. infantis C-3 and Bifidobacterium lactis CL-03 for relieving eczema as well as a preparation method and application of the probiotic agent Bifidobacterium longum subsp. infantis C-3 strain and Bifidobacterium lactis with a preservation number of GDMCC No.65725 Lacticaseibacillus rhamnosus The C-3 strain and the Bifidobacterium lactis CL-03 strain are combined. The prepared probiotic agent can relieve eczema, repair skin barrier and inhibit the proliferation of harmful bacteria on the skin, and the Bifidobacterium longum subsp. infantis C-3 strain and the Bifidobacterium lactis CL-03 strain have certain synergistic effect on the above effects.

Claims

1. A probiotic agent containing *Lactobacillus rhamnosus* CL-03 for relieving eczema, characterized in that, The probiotic strain in the probiotic agent is *Bifidobacterium longum* subsp. *infantitidis*, with accession number GDMCC No:64541. Bifidobacterium longum subsp.infantis Strain C-3 and Lactobacillus rhamnosus with accession number GDMCC No. 65725 Lacticaseibacillus rhamnosus Composition of CL-03 strain; Bifidobacterium longum infant subspecies Bifidobacterium longum subsp.infantis C-3 strain and Lactobacillus rhamnosus Lacticaseibacillus rhamnosus The ratio of the number of bacteria in strain CL-03 is (1-10):(1-10); The probiotic agent also includes acceptable excipients and prebiotics, wherein the prebiotics are xylooligosaccharides; The probiotic agent contains xylooligosaccharides at a mass percentage of 5-20%.

2. The probiotic agent for relieving eczema containing *Lactobacillus rhamnosus* CL-03 according to claim 1, characterized in that, The probiotic agent has a bacterial count of not less than 1×10⁻⁶. 9 cells / mL or 1×10 9 per g.

3. The probiotic agent for relieving eczema containing *Lactobacillus rhamnosus* CL-03 according to claim 1, characterized in that, The acceptable excipients include protective agents.

4. A method for preparing a probiotic agent for relieving eczema containing *Lactobacillus rhamnosus* CL-03 according to any one of claims 1-3, characterized in that, The preparation method includes: activating the bacterial strains separately and then fermenting them to obtain a fermentation broth; mixing the fermentation broth with a protective agent and freeze-drying it; and mixing the freeze-dried bacterial powder to obtain the final product.

5. The use of the probiotic agent containing Lactobacillus rhamnosus CL-03 for relieving eczema according to any one of claims 1-3 in the preparation of products for preventing or relieving eczema.

6. The use of the probiotic agent for relieving eczema containing Lactobacillus rhamnosus CL-03 according to any one of claims 1-3 in the preparation of products for repairing the skin barrier.

Images