Isolation and Screening Methods and Applications of Plant Immune-Activating DSE Fungi

Abstract

This invention relates to the field of microbial technology, and in particular to the isolation and screening method and application of plant immune-activated DSE fungi. Based on the isolation technology of "directed trapping + inhibition of contaminating bacteria + symbiosis assay", this invention improves the isolation rate and new species discovery rate of DSE fungi by 28 times and 7 times respectively compared with conventional direct isolation methods, significantly enhancing the exploration of fungal resources. Furthermore, the invention improves the inhibition ability of contaminating bacteria by modifying the isolation culture medium, further improving the fungal isolation rate. In addition, a comprehensive evaluation method is used, including strain-cucumber symbiosis assay, whole plant dry weight evaluation, and cucumber wilt disease in vivo plate assay, to screen DSE strains with plant immune activation function. By using in vivo samples instead of in vitro samples and indirectly activating plant immunity instead of directly inhibiting pathogens, this invention overcomes the limitations of traditional antagonistic methods that only target specific pathogens, achieving efficient identification of broad-spectrum induced resistant strains and providing new ideas and methods for screening biocontrol microorganisms.

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