A method for cultivating a mixed bacterial community that efficiently degrades PFAS and its application

CN122081118APending Publication Date: 2026-05-26SOUTH CHINA AGRICULTURAL UNIVERSITY

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Patent Information

Authority / Receiving Office: CN · China
Patent Type: Applications(China)
Current Assignee / Owner: SOUTH CHINA AGRICULTURAL UNIVERSITY
Filing Date: 2026-03-11
Publication Date: 2026-05-26

AI Technical Summary

Technical Problem

Existing microbial degradation technologies lack functional microbial communities capable of specifically and efficiently degrading PFAS and simultaneously achieving deep defluorination, and there is a lack of corresponding bioaugmentation strategies.

Method used

By selectively screening and constructing efficient and stable mixed microbial communities, including sulfate-reducing bacteria (SRB), and under optimized Fe2+ concentration and the addition of the reductive dehalogenase coenzyme factor VB12, the culture medium solution was enriched to achieve efficient degradation and deep defluorination of fluorochlorooctanoic acid.

Benefits of technology

It achieves efficient degradation and deep defluorination of fluorochlorooctanoic acid, improves the efficiency and effectiveness of microbial degradation of PFAS, and has good water and soil remediation capabilities.

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Abstract

This invention provides a method and application for the cultivation of a mixed bacterial community that efficiently degrades PFAS. The method includes preparing a culture medium, adding bacterial suspension to the medium to obtain a culture medium solution. The culture medium solution is placed in an anaerobic serum bottle, and nitrogen gas is introduced into the anaerobic serum bottle. Under conditions containing ferrous ions, sulfate-reducing bacteria in the culture medium solution are enriched to obtain an enriched bacterial solution. Sulfate-reducing bacteria (SRBs) possess potential defluorination capabilities; compared to obligate dehalogenating bacteria, sulfate-reducing bacteria exhibit stronger survival and metabolic abilities. This invention optimizes the Fe... 2+ Based on the concentration, add the reductive dehalogenase coenzyme cobalamin (VB). 12 This enables efficient degradation and deep defluorination of fluorochlorooctanoic acid (CTFE4).

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