A child formula milk for promoting bone development and a method of making the same

Abstract

This invention provides a children's formula milk that promotes bone development and its preparation method, relating to the field of dairy product technology. The children's formula milk for promoting bone development of this invention comprises the following raw materials in parts by weight: 970-999 parts raw milk, 0.05-1 part compound vitamins, 0.1-1.5 parts minerals, 0.1-1 part colostrum basic protein, and 0.1-2 parts 2'-fucosylated lactose; the compound vitamins include vitamin K, and the mass ratio of 2'-fucosylated lactose to the compound vitamins is 0.5:1-20:1; the mass ratio of colostrum basic protein: 2'-fucosylated lactose: vitamin K: minerals is 1:(1-10):(0.1-0.5):(0.5-10). The children's formula milk for promoting bone development of this invention, through the specific proportions of each raw material component, can effectively promote bone development while improving product stability.

Description

Technical Field

[0001] This invention relates to the field of dairy technology, and in particular to a children's formula milk that promotes bone development and its preparation method. Background Technology

[0002] Bone health is the core foundation of children's growth and development. Its development directly determines a child's height potential, physical health, and future risk of bone diseases, while adequate and precise nutritional supply is a key driver of bone development. Currently, to address the needs of children's bone health and calcium supplementation during growth and development, infant formula and modified dairy products typically contain a combination of functional ingredients that promote calcium absorption and bone development, such as calcium, vitamins, and colostrum basic proteins. While these ingredients can promote bone development to some extent, current technology still has significant shortcomings in multi-nutrient compound systems. As the types and amounts of bone health-related functional ingredients increase, the negative impact of the formulation system on taste, flavor, and stability becomes more pronounced. For example, some vitamins and minerals themselves have a distinct bitter, astringent, or unpleasant flavor; direct compounding can easily lead to flavor degradation, significantly reducing children's palatability and acceptance. Simultaneously, the coexistence of multiple active substances, minerals, and protein systems can easily lead to stability issues such as layering, flocculation, and precipitation, affecting product shelf life and edibility. Most existing technologies focus only on the superposition of nutritional effects, lacking systematic research on the synergistic improvement of flavor, bitterness suppression and system stability, making it difficult to achieve a balance between the taste, flavor and stability of the formula while ensuring the efficacy of bone development.

[0003] Therefore, developing a children's formula milk that can effectively promote children's bone development, significantly improve taste and flavor, and enhance product stability has important practical significance and application value. Summary of the Invention

[0004] This invention addresses the shortcomings of current children's formula milk products with nutritional benefits for promoting bone development, such as poor taste, flavor, and product stability. It provides a children's formula milk product that promotes bone development by using a specific nutritional ratio to solve the problems of taste and system stability, thereby improving the quality of children's formula milk products with nutritional benefits for promoting bone development and extending shelf life stability.

[0005] This invention provides a method for preparing infant formula milk that promotes bone development.

[0006] In a first aspect, the present invention provides a children's formula milk that promotes bone development, comprising the following ingredients in parts by weight: 970-999 parts of raw milk, 0.05-1 part of compound vitamins, 0.1-1.5 parts of minerals, 0.1-1 part of colostrum basic protein, and 0.1-2 parts of 2'-fucosylated lactose; The compound vitamin contains vitamin K, and the mass ratio of 2'-fucosylated lactose to the compound vitamin is 0.5~20:1; The mass ratio of the colostrum basic protein: 2'-fucosylated lactose: vitamin K: minerals is 1:(1~10):(0.1~0.5):(0.5~10).

[0007] The infant formula milk for promoting bone development protected by the present invention preferably further comprises vitamin B1 and vitamin B6 in the compound vitamin, wherein the mass ratio of vitamin B1 to vitamin B6 is (1~2):1.

[0008] In the bone-promoting formula milk for children protected according to the present invention, preferably, the vitamin B1 is derived from thiamine nitrate.

[0009] The infant formula milk for promoting bone development protected according to the present invention preferably further comprises vitamin D in the complex vitamin, wherein the mass ratio of vitamin D to vitamin K (based on pure product) is 1:1 to 10.

[0010] In the bone-promoting infant formula milk protected according to the present invention, preferably, the mass ratio of vitamin D to vitamin K (based on pure products) is 1:5 to 8.

[0011] In the infant formula milk for promoting bone development protected by the present invention, preferably, the mass ratio of colostrum basic protein to 2'-fucosylated lactose is 1:1 to 10.

[0012] The bone-promoting infant formula milk protected according to the present invention preferably has an average particle size of 0.2~0.3μm.

[0013] Secondly, the present invention also specifically protects a method for preparing infant formula milk that promotes bone development, comprising the following steps: S1. Vitamin K and minerals in the compound vitamins are processed with cold milk at 4~15℃ to obtain compound 1; 2'-fucosylated lactose, other vitamins in the compound vitamins and colostrum basic protein are processed with hot milk at 60~65℃ to obtain compound 2. S2. After mixing chemical material 1 and chemical material 2, deaerate, homogenize and sterilize.

[0014] According to the method for preparing infant formula milk that promotes bone development protected by the present invention, preferably, the vacuum degree of degassing in step S2 is -0.5 to -0.8 bar.

[0015] According to the method for preparing infant formula milk that promotes bone development protected by the present invention, preferably, the homogenization in S2 is a two-stage homogenization, the homogenization temperature is 60-65°C, the total homogenization pressure is 220-250 bar, and the secondary pressure is 20% of the total pressure.

[0016] Beneficial effects: This invention provides a children's formula milk that promotes bone development. It contains a complex nutritional composition of multivitamins, minerals, colostrum basic protein, and 2'-fucosylated lactose. The specific proportions of each component effectively promote bone development. The synergistic effect of 2'-fucosylated lactose and multivitamins effectively eliminates off-flavors caused by added vitamins. Using raw milk as the ingredient, without added white sugar, food flavorings, or stabilizers, the invention solves problems such as off-flavors and sedimentation during product processing simply by controlling the mass ratio of colostrum basic protein, 2'-fucosylated lactose, vitamin K, and minerals. This improves product stability, increases nutrient content within the shelf life, and balances formula cleanliness and taste compatibility, thus enhancing the quality of children's formula milk and achieving precise supplementation of key nutrients. Detailed Implementation

[0017] To make the objectives, technical solutions, and advantages of this invention clearer, the technical solutions of this invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of this invention, not all embodiments. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this invention.

[0018] In a specific embodiment, the present invention provides a children's formula milk that promotes bone development, comprising the following raw materials in parts by weight: 970-999 parts of raw milk, 0.05-1 part of compound vitamins, 0.1-1.5 parts of minerals, 0.1-1 part of colostrum basic protein, and 0.1-2 parts of 2'-fucosylated lactose; The compound vitamin contains vitamin K, and the mass ratio of 2'-fucosylated lactose to the compound vitamin is 0.5~20:1; The mass ratio of the colostrum basic protein: 2'-fucosylated lactose: vitamin K: minerals is 1:(1~10):(0.1~0.5):(0.5~10).

[0019] It should be noted that: In the bone-promoting infant formula milk of this invention, the raw milk base can provide sufficient nutrients (including calcium), the compound vitamins can promote the absorption and utilization of bone calcium, the colostrum basic protein can stimulate osteoblast growth, inhibit osteoclasts, and reduce bone loss, the 2'-fucosylated lactose can effectively improve the intestinal environment, promote the absorption of various nutrients in the infant formula milk, provide nutrition for bone development, and the minerals can participate in the bone mineralization process, increase bone density and bone strength, and comprehensively and synergistically promote bone development.

[0020] The children's formula milk of this invention, in combination with 2'-fucosylated lactose and complex vitamins, effectively solves the off-flavors caused by the addition of vitamins and other ingredients. It uses raw milk as an ingredient and does not add white sugar, food flavorings, stabilizers, or other ingredients. By controlling the mass ratio of colostrum basic protein, 2'-fucosylated lactose, vitamin K, and minerals, it solves the problems of nutrient off-flavors and system sedimentation during product processing, improves product stability, increases the nutrient content within the shelf life, and simultaneously ensures formula cleanliness and taste compatibility, thus improving the quality of children's formula milk.

[0021] In some specific embodiments, the mass ratio of 2'-fucosylated lactose to complex vitamins is 0.5:1 to 10:1.

[0022] In some specific embodiments, for example, the mass fraction of colostrum basic protein is 1 part, the mass fraction of 2'-fucosylated lactose can be 1 part, 3 parts, 5 parts, 8 parts, 10 parts, etc., the mass fraction of vitamin K can be 0.1 parts, 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, etc., and the mass fraction of minerals can be 0.5 parts, 1 part, 3 parts, 5 parts, 7 parts, 9 parts, 10 parts, etc.

[0023] In some specific embodiments, in order to further improve the palatability of children's formula milk, while meeting the vitamin nutritional content required to promote bone calcium absorption and taking into account the flavor and taste of children's formula milk, and synergistically reducing the bitterness of vitamins, the compound vitamins mentioned in this invention are further preferably composed of vitamin B1 and vitamin B6, wherein the mass ratio of vitamin B1 to vitamin B6 is (1~2):1.

[0024] In some specific exemplary embodiments, the mass ratio of vitamin B1 to vitamin B6 mentioned in this invention can be, for example, 1:1.

[0025] In some specific exemplary embodiments, the mass ratio of vitamin B1 to vitamin B6 mentioned in this invention may be, for example, 2:1.

[0026] In some preferred embodiments, in order to further improve the milk flavor of children's formula milk products, enhance the sensory quality of the products, maintain system stability, and prevent product fat from floating, the vitamin B1 mentioned in this invention is preferably derived from thiamine nitrate.

[0027] In a specific embodiment, the compound vitamin mentioned in this invention also contains vitamin D, and the mass ratio of vitamin D to vitamin K (based on pure products, the same below) is 1:0.1~20.

[0028] In some preferred embodiments, the mass ratio of vitamin D to vitamin K mentioned in this invention is 1:1 to 10.

[0029] A synergistic combination of vitamin D and vitamin K in a mass ratio of 1:1 to 10 can further enhance the bone development-promoting effects of infant formula milk and make it more conducive to the absorption and utilization of bone calcium.

[0030] In some specific exemplary embodiments, the mass ratio of vitamin D to vitamin K mentioned in this invention may be, for example, 1:10.

[0031] In some specific exemplary embodiments, the mass ratio of vitamin D to vitamin K mentioned in this invention may be, for example, 1:8.

[0032] In some specific exemplary embodiments, the mass ratio of vitamin D to vitamin K mentioned in this invention may be, for example, 1:5.

[0033] In some specific exemplary embodiments, the mass ratio of vitamin D to vitamin K mentioned in this invention may be, for example, 1:2.

[0034] In some specific exemplary embodiments, the mass ratio of vitamin D to vitamin K mentioned in this invention may be, for example, 1:1.

[0035] In specific embodiments, the mass ratio of vitamin D to vitamin K in the present invention can be the point value in the above exemplary embodiments or any range of values ​​thereof.

[0036] In a specific embodiment, the vitamin D mentioned in this invention may be derived from vitamin D3, and the vitamin K may be derived from vitamin K2.

[0037] In some preferred embodiments, the mass ratio of colostrum basic protein to 2'-fucosylated lactose mentioned in this invention is 1:1 to 10.

[0038] The synergistic regulation of colostrum basic protein and 2'-fucosylated lactose can further enhance the bone development-promoting effects of infant formula milk and make it more conducive to promoting bone calcium absorption and utilization.

[0039] Colostrum basic protein can stimulate osteoblast growth, inhibit osteoclasts, and reduce bone loss. 2'-fucosylated lactose can effectively improve the intestinal environment, promote the absorption of various nutrients in infant formula milk, and provide nutrition for bone development. The synergistic ratio of the two in 1:1 to 10 can achieve a better effect on promoting bone development.

[0040] In some specific exemplary embodiments, the mass ratio of colostrum basic protein to 2'-fucosylated lactose mentioned in this invention can be, for example, 1:1.

[0041] In some specific exemplary embodiments, the mass ratio of colostrum basic protein to 2'-fucosylated lactose mentioned in this invention can be, for example, 1:3.

[0042] In some specific exemplary embodiments, the mass ratio of colostrum basic protein to 2'-fucosylated lactose mentioned in this invention can be, for example, 1:5.

[0043] In some specific exemplary embodiments, the mass ratio of colostrum basic protein to 2'-fucosylated lactose mentioned in this invention can be, for example, 1:8.

[0044] In some specific exemplary embodiments, the mass ratio of colostrum basic protein to 2'-fucosylated lactose mentioned in this invention can be, for example, 1:10.

[0045] In specific embodiments, the mass ratio of colostrum basic protein to 2'-fucosylated lactose of the present invention can be the point value in the above exemplary embodiments or any range of values ​​thereof.

[0046] In specific embodiments, the purity of 2'-fucosylated lactose (dry basis) mentioned in this invention is greater than 94 wt%, for example, it can be 98 wt%, the purity of vitamin D3 (dry basis) mentioned in this invention is 0.25 wt%, and the purity of vitamin K2 (dry basis) is 0.2 wt%.

[0047] In a specific embodiment, the compound vitamins mentioned in the bone-development-promoting children's formula milk of the present invention include: vitamin A, vitamin K, vitamin D, vitamin B1, vitamin B6, and vitamin B1. 12 Folic acid and lutein.

[0048] The various vitamins in the compound vitamins mentioned in this invention can be derived from any source available in the art. The content of the compound vitamins per 100g of formula milk is the designed content of the formula. The preferred types and contents of the compound vitamins are shown in Table 1 below: Table 1. Sources and content of multivitamins In a specific embodiment, the minerals mentioned in the bone-promoting infant formula milk of the present invention include zinc, iron, magnesium, etc., wherein, based on 100g of formula milk, the preferred zinc content is 1.2~1.8mg, the iron content is 0.56~1.20mg, and the magnesium content is 0.5~20mg.

[0049] The minerals mentioned in this invention can be derived from any source available in the art, wherein zinc can be derived from zinc citrate, iron can be derived from sodium iron ethylenediaminetetraacetate, magnesium can be derived from magnesium oxide, etc.

[0050] In a specific embodiment, the present invention also provides a method for preparing infant formula milk that promotes bone development, comprising the following steps: S1. Vitamin K and minerals in the compound vitamins are processed with cold milk at 4~15℃ to obtain compound 1; 2'-fucosylated lactose, other vitamins in the compound vitamins and colostrum basic protein are processed with hot milk at 60~65℃ to obtain compound 2. S2. After mixing chemical material 1 and chemical material 2, deaerate, homogenize and sterilize.

[0051] In the preparation method provided by this invention, using cold milk processing at 4-15℃ for vitamin K and minerals avoids the oxidative decomposition and activity loss of vitamin K caused by high-temperature processing. Simultaneously, it reduces the flocculation and precipitation reactions of minerals with milk proteins under high-temperature conditions, lowering the risk of system instability. The low-temperature environment also effectively inhibits the oxidative deterioration of free fatty acids, reduces product odor, and better preserves the nutritional efficacy, taste, and system stability of the formula.

[0052] In some preferred embodiments, dissolved oxygen in S2 is a key factor leading to the oxidative degradation of B vitamins. Degassing can significantly reduce the oxidation rate of B vitamins, reduce the generation of off-odor substances, and improve the stability of the system. The present invention preferably controls the vacuum degree of the degassing process to -0.5 to -0.8 bar.

[0053] In some specific exemplary embodiments, the vacuum degree of degassing can be a point value such as -0.5 bar, -0.6 bar, -0.7 bar, -0.8 bar, or any range of values.

[0054] In some preferred embodiments, the homogenization temperature mentioned in S2 of the present invention is 60-65°C, the total homogenization pressure is 220-250 bar, and the secondary pressure is 20% of the total pressure.

[0055] Excessive homogenization pressure leads to excessive breakage of milk fat, increasing free fatty acids and exacerbating off-flavors through reaction with B vitamins. Conversely, insufficient homogenization temperature results in poor homogenization, causing product stratification and indirectly increasing the contact between vitamins and oxygen. Considering the specific infant formula milk raw material formulation of this invention, and to achieve better overall system taste and flavor improvement and system stability, while obtaining a smaller average milk particle size, this invention requires degassing treatment. The vacuum degree of degassing treatment is -0.5 to -0.8 bar. The preferred homogenization pressure is a total homogenization pressure of 220 to 250 bar, with a secondary pressure of 20% of the total pressure, and a homogenization temperature of 60 to 65°C.

[0056] In some specific exemplary embodiments, the method for preparing the bone-promoting infant formula milk mentioned in this invention may include the following steps: Preparation 1: Pour 4-15℃ cold milk into the preparation tank, add vitamin K2 and minerals, and stir with a stirrer for 15 minutes; 2. Add milk to the milk preparation tank and heat it to 60-65°C. Add 2'-FL, vitamins and CBP while circulating the milk. Keep it for 10-15 minutes. After the milk preparation is complete, cool it down. The cooling temperature should be ≤10°C. Mix ingredients 1 and 2, stir for 10 minutes, mix with the remaining cold milk in the formula and bring to a final volume. Then bring to a final volume using milk (2~6℃) to obtain the infant formula milk liquid. Degassing: Degassing parameters are -0.3 to -0.8 bar, and the temperature is 60-65℃; Homogenization and sterilization: UHT (ultra-high temperature instantaneous sterilization) sterilization (137±2℃ / 4-6s): preheating temperature 60~70℃; homogenization temperature 60~65℃; total homogenization pressure: 220~250bar; secondary pressure: 20% of total pressure.

[0057] In actual production, the obtained infant formula milk powder is cooled to 10~15℃ and then filled to obtain the finished infant formula milk powder.

[0058] In a specific embodiment, the children's formula milk prepared by the method of the present invention has an average particle size of 0.2~0.3μm, which has a small particle size and good stability, and no fat floating or sedimentation during the shelf life.

[0059] The compound vitamins in this embodiment of the invention include vitamin A, vitamin D, vitamin K, vitamin B1, vitamin B6, and vitamin B1. 12 Folic acid and lutein, their content referenced relevant standard values. Vitamin A is derived from vitamin A acetate, vitamin D from vitamin D3, vitamin K from vitamin K2, vitamin B1 from thiamine nitrate, and vitamin B6 from pyridoxine hydrochloride. 12It is derived from cyanocobalamin.

[0060] The minerals include zinc, iron, and magnesium, with zinc derived from zinc citrate, iron from sodium iron ethylenediaminetetraacetate, and magnesium from magnesium oxide.

[0061] Example 1 A children's formula milk that promotes bone development, comprising, by weight, the following ingredients: 998.12 parts raw milk, 0.18 parts compound vitamins (0.03 parts vitamin K2), 0.2 parts minerals, 0.25 parts colostrum basic protein (CBP), and 1.25 parts 2'-fucosylated lactose (2'-FL); The mass ratio of 2'-fucosylated lactose to multivitamins is 7:1; The mass ratio of colostrum basic protein: 2'-fucosylated lactose: vitamin K2: minerals is 1:5:0.12:0.8.

[0062] The mass ratio of vitamin B1 to vitamin B6 in a multivitamin is 2:1.

[0063] The mass ratio of vitamin D3 to vitamin K2 (based on pure products) is 1:8.

[0064] This embodiment 1 also provides a method for preparing infant formula milk that promotes bone development, including the following steps: S1. Pour 4-15℃ cold milk into a mixing tank, add vitamin K2 and minerals, and stir with a mixer for 15 minutes to obtain mixing material 1; Milk was pumped into the mixing tank and heated to 62°C. 2'-FL, vitamins and CBP were added in circulation and kept for 12 minutes. After mixing, the mixture was cooled at 10°C to obtain mixture 2. S2. Mix ingredients 1 and 2, stir for 10 minutes, mix with the remaining cold milk in the formula and bring to a final volume. Use milk (2-6℃) to bring to a final volume to obtain the infant formula milk liquid. Degassing: Degassing parameters are -0.5 bar and temperature 65℃; Homogenization and sterilization: Preheating temperature 60-70℃, homogenization temperature 60℃, total homogenization pressure: 240 bar, secondary pressure: 20% of total pressure, UHT sterilization (137±2℃ / 4-6s).

[0065] Example 2 A children's formula milk that promotes bone development, comprising, by weight, the following ingredients: 997.8 parts raw milk, 0.25 parts multivitamin (0.05 parts vitamin K2), 0.3 parts minerals, 0.15 parts colostrum basic protein (CBP), and 1.5 parts 2'-fucosylated lactose (2'-FL); The mass ratio of 2'-fucosylated lactose to multivitamins is 6:1; The mass ratio of colostrum basic protein: 2'-fucosylated lactose: vitamin K2: minerals is 1:10:0.3:2.

[0066] The mass ratio of vitamin B1 to vitamin B6 in a multivitamin is 2:1.

[0067] The mass ratio of vitamin D3 to vitamin K2 (based on pure products) is 1:8.

[0068] The preparation method of the bone-promoting infant formula milk in Example 2 is the same as that in Example 1.

[0069] Example 3 A children's formula milk that promotes bone development, comprising, by weight, the following ingredients: 998.2 parts raw milk, 0.50 parts multivitamin (0.04 parts vitamin K2), 0.5 parts minerals, 0.4 parts colostrum basic protein (CBP), and 0.4 parts 2'-fucosylated lactose (2'-FL); The mass ratio of 2'-fucosylated lactose to multivitamins is 0.8:1; The mass ratio of colostrum basic protein: 2'-fucosylated lactose: vitamin K2: minerals is 1:1:0.1:1.2.

[0070] The mass ratio of vitamin B1 to vitamin B6 in a multivitamin is 2:1.

[0071] The mass ratio of vitamin D3 to vitamin K2 (based on pure products) is 1:8.

[0072] The preparation method of the bone-promoting infant formula milk in Example 3 is the same as that in Example 1.

[0073] Example 4 A children's formula milk that promotes bone development, comprising, by weight, the following ingredients: 995.82 parts raw milk, 0.88 parts compound vitamins (0.08 parts vitamin K2), 1.5 parts minerals, 0.2 parts colostrum basic protein (CBP), and 1.6 parts 2'-fucosylated lactose (2'-FL); The mass ratio of 2'-fucosylated lactose to multivitamins is 1.8:1; The mass ratio of colostrum basic protein: 2'-fucosylated lactose: vitamin K2: minerals is 1:8:0.4:6.

[0074] The mass ratio of vitamin B1 to vitamin B6 in a multivitamin is 2:1.

[0075] The mass ratio of vitamin D3 to vitamin K2 (based on pure products) is 1:8.

[0076] The preparation method of the children's formula milk that promotes bone development in Example 4 is the same as that in Example 1.

[0077] Example 5 A children's formula milk that promotes bone development, comprising, by weight, the following ingredients: 997.8 parts raw milk, 0.1 parts multivitamin (0.1 parts vitamin K2), 0.5 parts minerals, 0.5 parts colostrum basic protein (CBP), and 1 part 2'-fucosylated lactose (2'-FL); The mass ratio of 2'-fucosylated lactose to multivitamins is 5:1; The mass ratio of colostrum basic protein: 2'-fucosylated lactose: vitamin K2: minerals is 1:2:0.2:1.

[0078] The mass ratio of vitamin B1 to vitamin B6 in a multivitamin is 2:1.

[0079] The mass ratio of vitamin D3 to vitamin K2 (based on pure products) is 1:8.

[0080] The preparation method of the bone-promoting formula milk for children in Example 5 is the same as that in Example 1.

[0081] Example 6 A children's formula milk that promotes bone development is basically the same as in Example 2, except that the vitamin B1 is derived from thiamine hydrochloride.

[0082] Example 7 A children's formula milk that promotes bone development is basically the same as that in Example 1, except that the ratio of vitamin B1 to vitamin B6 is 3:1.

[0083] Experimental Example 1 Experiment on the effects of different ratios of vitamin D3 to vitamin K2 on promoting bone development.

[0084] The efficacy of promoting bone development was verified through zebrafish experiments. The specific experimental steps are as follows: 1) Sample preparation The solvents for vitamin K2 and vitamin D3 were standard dilution water (containing 294.0 mg / L CaCl2·2H2O, i.e. 80.0 μg / mL calcium, corresponding to 0.48 g / day for humans; this calcium was naturally present in the experimental environment).

[0085] The detected concentrations of vitamin K2 and vitamin D3 were 1.80 ~ 461 ng / mL and 0.667 ~ 54.0 ng / mL, respectively. According to the patent CN113496071B "Conversion Method for Zebrafish to Human Dosage for Efficacy Evaluation" published by Huante, the corresponding daily human doses are 10.8 ~ 2766 μg / day and 4.00 ~ 324 μg / day, respectively.

[0086] Positive control: Swisse "Calcium & Vitamin D", milky white tablets, batch number 33152A, Swisse, solvent is standard dilution water.

[0087] 2) Laboratory animals Zebrafish were raised in aquarium water at 28℃ (water quality: 200 mg of readily soluble sea salt added per 1 L of reverse osmosis water; conductivity 450~550 μS / cm; pH 6.5~8.5; hardness 50~100 mg / L CaCO3), bred and provided by Huante Biological Fish Farming Center. The laboratory animal use license number is: SYXK (Zhejiang) 2022-0004. The husbandry and management met the requirements of international AAALAC certification (certification number: 001458), and the IACUC ethics review number is: IACUC-2025-202508290008-01.

[0088] 3) Instruments, consumables and reagents Dissecting microscope (SZX7, OLYMPUS, Japan); CCD camera (VertA1, Shanghai Tusen Vision Technology Co., Ltd., China); Precision electronic balance (CP214, OHAUS, USA); Motorized focusing continuous zoom fluorescence microscope (AZ100, Nikon, Japan); Fully automated rapid sample grinder (JXFSTPRP-24L, Shanghai Jingxin Experimental Equipment Technology Department, China).

[0089] Methylcellulose (batch number E228154, Shanghai Aladdin Biochemical Technology Co., Ltd., China).

[0090] 4) Detection methods 30 transgenic bony fluorescent zebrafish (3 dpf) were randomly selected and placed in beakers. Samples were administered via water-soluble administration (concentrations shown in Table 2). A positive control group received Swisse "Calcium & Vitamin D" at a concentration of 500 μg / mL. A normal control group was also included. Each beaker contained 20 mL of serum. After treatment at 28℃ for 3 days, 10 zebrafish from each experimental group were randomly selected and photographed under a fluorescence microscope. Data were analyzed and collected using NIS-ElementsD 3.20 advanced image processing software. The osteogenic fluorescence intensity of the zebrafish was analyzed, and the statistical analysis results were used to evaluate the efficacy of the samples in promoting bone development. Statistical results are expressed as mean ± SE. Statistical analysis was performed using SPSS software; p < 0.05 indicated statistical significance.

[0091] 5) Statistical methods Statistical analysis was performed using SPSS software. A p-value < 0.05 was considered statistically significant. The statistical methods are as follows: a. Statistical analysis among multiple groups: Explore the data and obtain the exploration results using the KS test. If the normality test p>0.05 and the variance test p>0.05, use one-way ANOVA analysis in the parametric tests for statistical analysis; if either the normality test or the variance test p<0.05, that is, it is determined that the data does not follow a normal distribution or has unequal variances, then use independent samples in the nonparametric tests for statistical analysis. b. Statistical analysis between two groups: Explore the data and obtain the exploration results by KS test. If the normality test p>0.05 (i.e., it follows a normal distribution), then use the independent samples T test in the parametric test for statistical analysis; if the normality test p<0.05 (i.e., it does not follow a normal distribution), then use the two independent samples in the nonparametric test for statistical analysis.

[0092] Note: For n ≥ 10, the data needs to be explored before statistical analysis. For n < 10, the data can be directly analyzed using parametric tests. Only the difference between statistical analysis of multiple groups and statistical analysis of two groups needs to be distinguished.

[0093] The specific experimental results are shown in Table 2.

[0094] Table 2. Results of zebrafish experiments with different ratios of vitamin D3 to vitamin K2. Compared with the normal control group, p<0.001; compared with combination 1, # p<0.05, ## p<0.01, ###p<0.001; compared with combination 2, aa p<0.01; compared with combination 3, bb p<0.01, bbb p<0.001; compared with combination 6, cc p<0.01; compared with combination 7, ^^ p<0.01; compared with combination 8, !! p<0.01, !!! p<0.001; compared with vitamin D3 3.00 ng / mL, Compared with vitamin K2 3.00 ng / mL, & p<0.05, && p<0.01, &&& p<0.001.

[0095] The results in Table 2 show that combinations 1-8, vitamin D3 (3.00 ng / mL), and vitamin K2 (3.00 ng / mL) all promote bone development, specifically by increasing osteogenic fluorescence intensity. Among these, combination 1 showed no statistically significant difference in its bone-development-promoting effect compared to combinations 7 and 8, and its vitamin D3 and vitamin K2 concentrations (3.00 ng / mL), but was lower than combinations 2-6. This indicates that the preferred vitamin D3 to vitamin K2 mass ratio (based on pure products) of 1:1-10 in this invention effectively promotes bone development.

[0096] Experimental Example 2 Experiments were conducted to investigate the bone development-promoting effects of different CBP to 2'-FL ratios. The results were verified using the same zebrafish experiments as in Example 9. Specific results are shown in Table 3.

[0097] Table 3. Results of zebrafish experiments with different ratios of CBP to 2'-FL Compared with the normal control group, p<0.05, p<0.01, p<0.001; compared with combination 1, ## p<0.01; compared with combination 5, Compared to combination 7, ^ p<0.05; compared with combination 8, ! p<0.05, !! p<0.01, !!! p<0.001; compared with 2'-FL 100 μg / mL, & p<0.05, &&& p<0.001.

[0098] Table 3 shows that combinations 1-8 and 100 μg / mL of 2'-FL all promoted bone development, specifically by increasing osteogenic fluorescence intensity, while 100 μg / mL of CBP did not significantly increase osteogenic fluorescence intensity. Combination 1 showed better bone development promotion than 100 μg / mL of CBP, but showed no statistically significant difference compared to combinations 2, 7, 8, and 100 μg / mL of 2'-FL, although it was lower than combinations 3-6. This indicates that a preferred ratio of CBP to 2'-FL of 1:1-10 is preferred in this invention to effectively promote bone development.

[0099] Comparative Example 1 A children's formula milk that promotes bone development is basically the same as that in Example 3, except that it contains 0.9 parts of compound vitamins, 0.1 parts of vitamin K2, and 0.1 parts of 2'-fucosylated lactose, with a mass ratio of 2'-fucosylated lactose to compound vitamins of 0.1:1.

[0100] Comparative Example 2 A children's formula milk that promotes bone development is basically the same as that in Example 1, except that the mass ratio of 2'-fucosylated lactose to complex vitamins is 25:1.

[0101] Comparative Example 3 A method for preparing a children's formula milk that promotes bone development is basically the same as in Example 1, except that the total homogenization pressure is 350 bar.

[0102] Comparative Example 4 A children's formula milk that promotes bone development is basically the same as that in Example 2, except that it contains 0.45 parts of colostrum basic protein (CBP), 1.5 parts of 2'-fucosylated lactose (2'-FL), 0.05 parts of vitamin K2, and 0.3 parts of minerals, with a mass ratio of colostrum basic protein: 2'-fucosylated lactose: vitamin K2: minerals of 3:10:0.3:2.

[0103] Comparative Example 5 A method for preparing a children's formula milk that promotes bone development is basically the same as that in Example 1, except that 2'-FL, vitamins, CBP vitamin K2 and minerals are all heated and milked at 62°C.

[0104] Finished product inspection example Twelve professional sensory evaluators were selected to conduct descriptive tests on the same batch of example samples and comparative samples. The results are shown in Table 4.

[0105] Table 4. Sensory evaluation results of the infant formula milk samples from the examples and comparative cases. In Table 4, the appearance indicators are as follows: Surface whiteness (0-9 points) represents the color from light to dark compared to pure milk, with 6.5±0.2 points indicating a color close to pure milk. (Smell) Overall aroma and flavor intensity (0-9 points) represent the milk flavor from light to strong, with 6.5±0.2 points indicating a moderately strong milk flavor. Sweetness (0-9 points) represents the sweetness from not sweet to very sweet, with higher scores indicating higher sweetness, and 5.0±0.2 points indicating just the right amount of sweetness. Bitterness (0-5 points) represents the bitterness, with 0-0.3 points indicating no bitterness and higher scores indicating more pronounced bitterness. Smoothness (0-9 points) represents the smoothness from rough to smooth, with 6.5±0.2 points indicating optimal smoothness. Off-odors gradually increase from 0-5 points, with 5 points indicating the strongest off-odor.

[0106] As can be seen from Table 4 above, the infant formula milk products of Examples 1-5 of the present invention have good appearance indicators and moderate flavor indicators. Among them, Example 6 changed the source of vitamin B1 compound from Example 2, and it was derived from thiamine hydrochloride. The product characteristics are weaker milk aroma, poorer smoothness, and poorer sensory quality than Example 2.

[0107] Compared with Example 1, Example 7 had a vitamin B1:vitamin B6 ratio of 3:1, and the product exhibited bitterness and poor smoothness.

[0108] Compared with Example 3, Comparative Example 1 had a mass ratio of 2'-fucosylated lactose to complex vitamins of 0.1:1. The product exhibited a characteristic milky aroma, weaker overall flavor and sweetness, bitterness, strong off-flavor of B vitamins, and poor smoothness.

[0109] Compared with Example 1, Comparative Example 2 had a mass ratio of 2'-fucosylated lactose to complex vitamins of 25:1, and the product had a weaker milky aroma and overall flavor.

[0110] Compared to Example 1, Comparative Example 3 used a homogenization pressure of 350 bar. Excessive pressure can lead to excessive breakdown of milk fat, increased free fatty acids, and a reaction with B vitamins that exacerbates off-flavors.

[0111] Compared with Example 2, Comparative Example 4 had a mass ratio of colostrum basic protein: 2'-fucosylated lactose: vitamin K2: minerals of 3:10:0.3:2, and the product taste was not much different.

[0112] Compared with Example 1, Comparative Example 5, which contains 2'-FL, vitamins, CBP vitamin K2, and minerals, is a heat-treated milking agent. The product has a weaker milky aroma, a noticeable off-odor, and poorer sensory quality.

[0113] Stability and particle size testing The particle size and stability of the infant formula milk products obtained in the examples and comparative examples were tested respectively.

[0114] The testing method is as follows: The products obtained in each embodiment and comparative example were subjected to particle size and stability tests. The particle size was determined using a Beckman LS 13320 laser diffraction particle size analyzer, and the stability was determined using a LUM stability analyzer. The test results are shown in Table 5.

[0115] Table 5. Stability test results of the examples and comparative examples As can be seen from the data in Table 5, the infant formula milk of Examples 1-5 of the present invention has a small particle size and good stability.

[0116] In Example 6, the vitamin B1 compound derived from thiamine hydrochloride was changed compared to Example 2. The particle size and stability values ​​of the product were larger than those of Example 2, and the product stability was worse than that of Example 2.

[0117] In Example 7, compared to Example 1, the ratio of vitamin B1 to vitamin B6 was 3:1. The particle size and stability values ​​of the product were larger than the corresponding values ​​in Example 1, and the product stability was worse than that in Example 1.

[0118] Compared with Example 1, Comparative Example 1 had a larger particle size and a higher stability clarification index, resulting in a larger mass ratio of 2'-fucosylated lactose to complex vitamins of 0.1:1 and poorer stability.

[0119] Compared with Example 1, Comparative Example 2 had a mass ratio of 2'-fucosylated lactose to complex vitamins of 25:1. The particle size and stability values ​​of the product were larger than those of Example 1, and the product stability was worse than that of Example 1.

[0120] Compared to Example 1, Comparative Example 3 used a homogenization pressure of 350 bar. Excessive pressure leads to excessive breakage of milk fat, resulting in significant fat floating in the product, large particle size, a high clarification index, and poor stability.

[0121] Compared with Example 2, Comparative Example 4 had a mass ratio of colostrum basic protein: 2'-fucosylated lactose: vitamin K2: minerals of 3:10:0.3:2. The product had severe precipitation, large particle size, high stability clarification index, and poor stability.

[0122] Compared with Example 1, Comparative Example 5, which was homogenized with 2'-FL, vitamins, CBP vitamin K2, and minerals, showed severe fat floating and sedimentation in the product, large particle size, high stability clarification index, and poor stability.

[0123] Shelf-life product system stability observation record Table 6 shows the product system stability records of the children's formula milk in the embodiments and comparative examples of the present invention during the shelf life.

[0124] Table 6. Shelf-life stability test results of the examples and comparative examples

[0125] The recorded results show that the product systems of Examples 1-5 were stable, and no fat floated or precipitated during the shelf life.

[0126] In Example 6, the source of the vitamin B1 compound was changed from that in Example 2 to thiamine hydrochloride. The product's stability was slightly worse than that in Example 2 as the storage time increased.

[0127] In Example 7, compared to Example 1, the ratio of vitamin B1 to vitamin B6 was 3:1, and the product's stability was slightly worse than that of Example 1 as the storage time increased.

[0128] Compared with Example 1, Comparative Example 1 had a mass ratio of 2'-fucosylated lactose to complex vitamins of 0.1:1, resulting in severe fat floating and poor stability of the product.

[0129] Compared with Example 1, Comparative Example 2 had a mass ratio of 2'-fucosylated lactose to complex vitamins of 25:1, and the product's stability was slightly worse than that of Example 1 as the storage time increased.

[0130] Compared to Example 1, Comparative Example 3 used a homogenization pressure of 350 bar. Excessive pressure leads to excessive breakdown of milk fat, resulting in significant fat floating in the product and poor stability.

[0131] Compared with Example 2, Comparative Example 4 had a mass ratio of colostrum basic protein: 2'-fucosylated lactose: vitamin K2: minerals of 3:10:0.3:2, resulting in severe product precipitation and poor stability.

[0132] Compared with Example 1, Comparative Example 5, which used homogenized emulsified 2'-FL, vitamins, CBP vitamin K2, and minerals, showed severe fat floating and sedimentation in the product, resulting in poor stability.

[0133] Table 7 shows the nutritional composition of the infant formula milk in the embodiments and comparative examples of the present invention during its shelf life.

[0134] Table 7. Nutritional Information of Infant Formula Milk Products During Shelf Life in Examples and Comparative Cases As can be seen from the results in Table 7, the nutrient content of the infant formula milk in the embodiments of the present invention remained at the same level during the shelf life, which is consistent with the theoretical design values. In Example 6, the vitamin B1 compound was derived from thiamine hydrochloride, resulting in a lower vitamin B1 content in the product. Compared with Example 1, Example 7 had a vitamin B1:vitamin B6 ratio of 3:1, indicating a loss of B vitamins and a lower B vitamin content in the product.

[0135] Compared with Example 1, Comparative Example 1 had a mass ratio of 2'-fucosylated lactose to complex vitamins of 0.1:1. Due to the occurrence of fat floating and precipitation, the product suffered a serious loss of vitamins and minerals.

[0136] Compared to Example 1, Comparative Example 2 had a 2'-fucosylated lactose to complex vitamin mass ratio of 25:1, and the product nutrients were not significantly different from Example 1. Comparative Example 3 used a homogenization pressure of 350 bar, compared to Example 1. Excessive pressure led to excessive milk fat breakdown, severe fat floating in the product, and significant vitamin loss. Comparative Example 4, compared to Example 2, had a colostrum basic protein: 2'-fucosylated lactose: vitamin K2: mineral mass ratio of 3:10:0.3:2, and the product nutrients were not significantly different from Example 2. Comparative Example 5, compared to Example 1, used homogenized milk containing 2'-FL, vitamins, CBP vitamin K2, and minerals, resulting in severe fat floating and sedimentation in the product, and significant mineral loss.

[0137] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A children's formula milk that promotes bone development, characterized in that, The ingredients include the following parts by weight: 970-999 parts raw milk, 0.05-1 part compound vitamins, 0.1-1.5 parts minerals, 0.1-1 part colostrum basic protein, and 0.1-2 parts 2'-fucosylated lactose; The compound vitamin contains vitamin K, and the mass ratio of 2'-fucosylated lactose to the compound vitamin is 0.5~10:1; The compound vitamin also contains vitamin B1 and vitamin B6, wherein the mass ratio of vitamin B1 to vitamin B6 is (1~2):1, and the vitamin B1 is derived from thiamine nitrate. The complex vitamin also contains vitamin D, and the mass ratio of vitamin D to vitamin K is 1:1 to 10. The mass ratio of the colostrum basic protein: 2'-fucosylated lactose: vitamin K: minerals is 1:(1~10):(0.1~0.5):(0.5~10). The average particle size of the infant formula milk is 0.2~0.3μm; The preparation method of the infant formula milk that promotes bone development includes the following steps: S1. Vitamin K and minerals in the compound vitamins are processed with cold milk at 4~15℃ to obtain compound 1; 2'-fucosylated lactose, other vitamins in the compound vitamins and colostrum basic protein are processed with hot milk at 60~65℃ to obtain compound 2. S2. After mixing chemical material 1 and chemical material 2, deaerate, homogenize, and sterilize. The homogenization described in S2 is a two-stage homogenization, with a homogenization temperature of 60-65°C and a total homogenization pressure of 220-250 bar. The secondary pressure is 20% of the total pressure.

2. The infant formula milk for promoting bone development according to claim 1, characterized in that, The mass ratio of vitamin D to vitamin K is 1:5~8.

3. The infant formula milk for promoting bone development according to claim 2, characterized in that, The vitamin D mentioned is derived from vitamin D3, and the vitamin K is derived from vitamin K2.

4. The infant formula milk for promoting bone development according to claim 3, characterized in that, The mass ratio of vitamin D3 to vitamin K2 is 1:

8.

5. The infant formula milk for promoting bone development according to claim 1, characterized in that, The mass ratio of colostrum basic protein to 2'-fucosylated lactose is 1:3~8.

6. The infant formula milk for promoting bone development according to claim 5, characterized in that, The mass ratio of colostrum basic protein to 2'-fucosylated lactose is 1:

8.

7. A method for preparing infant formula milk that promotes bone development, characterized in that, Includes the following steps: S1. Vitamin K and minerals in the compound vitamins are processed with cold milk at 4~15℃ to obtain compound 1; 2'-fucosylated lactose, other vitamins in the compound vitamins and colostrum basic protein are processed with hot milk at 60~65℃ to obtain compound 2. S2. After mixing chemical material 1 and chemical material 2, degas, homogenize, and sterilize the mixture. The homogenization is a two-stage homogenization. The homogenization temperature is 60-65℃, the total homogenization pressure is 220-250 bar, and the secondary pressure is 20% of the total pressure. The raw material composition by weight is as follows: 970-999 parts raw milk, 0.05-1 part compound vitamins, 0.1-1.5 parts minerals, 0.1-1 part colostrum basic protein, and 0.1-2 parts 2'-fucosylated lactose; The compound vitamin contains vitamin K, and the mass ratio of 2'-fucosylated lactose to the compound vitamin is 0.5~10:1; The compound vitamin also contains vitamin B1 and vitamin B6, wherein the mass ratio of vitamin B1 to vitamin B6 is (1~2):1, and the vitamin B1 is derived from thiamine nitrate. The complex vitamin also contains vitamin D, and the mass ratio of vitamin D to vitamin K is 1:1 to 10. The mass ratio of the colostrum basic protein: 2'-fucosylated lactose: vitamin K: minerals is 1:(1~10):(0.1~0.5):(0.5~10). The average particle size of the infant formula milk is 0.2~0.3μm.

8. The method for preparing infant formula milk that promotes bone development according to claim 7, characterized in that, The vacuum degree of degassing described in S2 is -0.5 to -0.8 bar.

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