Collagen eye drop containing multiple plant extracts and preparation method thereof
By combining multiple plant extracts with recombinant type III humanized collagen, a multi-target ocular surface care system is constructed, which solves the problems of single function and poor biocompatibility of existing eye drops, and achieves highly effective anti-inflammatory, soothing and moisturizing effects, making it suitable for people with sensitive eyes.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- GANSU KANGSHILI CONTACT LENS CO LTD
- Filing Date
- 2026-04-24
- Publication Date
- 2026-05-29
AI Technical Summary
Existing eye drops have limited functions, few types of plant extracts, and unreasonable formulations. They also contain animal-derived collagen, which leads to high immunogenicity and poor biocompatibility. Long-term use may cause allergic reactions or irritation to the ocular surface.
It uses a combination of multiple plant extracts and recombinant type III humanized collagen, including honeysuckle, chrysanthemum, salvia miltiorrhiza, cassia seed, pearl powder, rosemary, peppermint, and goji berry, combined with sodium hyaluronate and osmotic pressure regulators to form a multi-target ocular surface care system, avoiding the potential risks of animal-derived collagen.
It achieves comprehensive conditioning of the ocular surface microenvironment, with multiple effects such as anti-inflammatory, soothing, repairing and moisturizing, and improves biocompatibility. It is suitable for people with sensitive eyes and significantly relieves symptoms such as dry eyes, eye strain and eye fatigue.
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Abstract
Description
Technical Field
[0001] This invention relates to the field of eye care technology, and in particular to a collagen-based eye drop containing multiple plant extracts and its preparation method. Background Technology
[0002] With the widespread use of electronic devices, dry eye syndrome has become a common problem affecting the eye health of modern people. In particular, young people who spend long hours in front of computer and mobile phone screens are prone to excessive tear evaporation, micro-inflammation of the ocular surface, and decreased tear film stability, which in turn leads to symptoms such as dry eyes, eye irritation, and eye strain.
[0003] Most commercially available eye drops currently rely on single moisturizing ingredients, such as sodium hyaluronate and polyvinyl alcohol. Their functions are relatively limited, primarily providing temporary lubrication and relief from dryness, lacking the ability to repair the ocular microenvironment and offering anti-inflammatory and soothing effects. While some products may contain small amounts of plant extracts, the limited variety and improper formulation make it difficult to achieve synergistic effects across multiple targets.
[0004] Furthermore, most collagen used in existing eye drops is derived from animal sources, which has issues such as high immunogenicity and poor biocompatibility. Long-term use may trigger allergic reactions or ocular irritation. Additionally, some products use preservatives to extend shelf life, further increasing the risk of ocular irritation, making them particularly unsuitable for people with sensitive eyes.
[0005] Therefore, it is of great significance to develop an eye drop with multiple effects such as anti-inflammatory, soothing, repairing and moisturizing, high biocompatibility and preservative-free system suitable for sensitive eyes. Summary of the Invention
[0006] In view of this, the present invention provides a collagen eye drop containing multiple plant extracts and its preparation method, in order to solve the problems of existing eye drops having limited functions, few types of plant extracts, unreasonable ratios, high immunogenicity, and poor biocompatibility.
[0007] To achieve the above objectives, the present invention adopts the following technical solution: This invention provides a collagen-based eye drop containing multiple plant extracts, comprising the following components by weight percentage: Recombinant type III humanized collagen 0.05-0.2%, honeysuckle extract 0.1-0.3%, chrysanthemum extract 0.1-0.25%, salvia miltiorrhiza extract 0.03-0.1%, cassia seed extract 0.05-0.12%, pearl powder extract 0.05-0.15%, rosemary extract 0.03-0.1%, peppermint extract 0.01-0.05%, wolfberry extract 0.08-0.2%, sodium hyaluronate 0.1-0.3%, glycerin 0.5-1.5%, dipotassium glycyrrhizate 0.05-0.15%, polysorbate 80 0.1-0.5%, disodium EDTA 0.01-0.05%, balance water.
[0008] Preferably, the collagen eye drops containing multiple plant extracts further include an osmotic pressure regulator, the amount of which is such that the osmotic pressure of the collagen eye drops containing multiple plant extracts is 280~320mOsm / L.
[0009] Preferably, the osmotic pressure regulator includes one or more of sodium chloride, potassium chloride, propylene glycol, and sorbitol.
[0010] Preferably, the collagen eye drops containing multiple plant extracts further include a pH adjuster, wherein the amount of the pH adjuster is such that the pH of the collagen eye drops containing multiple plant extracts is 6.5 to 7.5.
[0011] Preferably, the pH adjuster includes boric acid and / or disodium phosphate.
[0012] This invention also provides a method for preparing the above-mentioned collagen eye drops containing multiple plant extracts, comprising the following steps: 1) Mix sodium hyaluronate, glycerin, and the first portion of water to obtain mixture A; 2) Mix honeysuckle extract, chrysanthemum extract, salvia miltiorrhiza extract, cassia seed extract, pearl powder extract, rosemary extract, peppermint extract, wolfberry extract, dipotassium glycyrrhizate, polysorbate 80, disodium EDTA and water to obtain mixture B; 3) Mix recombinant type III humanized collagen, mixture A, mixture B, osmotic pressure regulator, pH regulator and water (part 3) to obtain collagen eye drops containing multiple plant extracts; There is no requirement for the order of steps 1) and 2).
[0013] Preferably, the mass ratio of the first part of water, the second part of water, and the third part of water is 1~2:4~6:1~2.
[0014] Preferably, the mixing temperature in step 1) is 60~90℃; the mixing temperatures in steps 2) and 3) are independently 20~30℃.
[0015] As can be seen from the above technical solution, compared with the prior art, the beneficial effects of the present invention are as follows: 1. This invention combines recombinant type III humanized collagen (a human homologous component) with extracts from various plants, including honeysuckle, chrysanthemum, salvia miltiorrhiza, cassia seed, pearl powder, rosemary, peppermint, and goji berry, to construct a four-in-one ocular surface care system encompassing "repair, anti-inflammation, soothing, and anti-oxidation." Each plant extract targets multiple areas, including anti-inflammation (e.g., honeysuckle and chrysanthemum), anti-oxidation (e.g., salvia miltiorrhiza and rosemary), and promoting repair (e.g., pearl powder and cassia seed). Peppermint extract and dipotassium glycyrrhizate provide an additional immediate cooling and soothing effect on top of anti-inflammatory properties. Goji berry, rich in polysaccharides and zeaxanthin, offers both moisturizing and antioxidant / ocular surface protection functions. Furthermore, the synergistic effect of the plant extracts, the cell regeneration function of recombinant collagen, and the potent moisturizing function of sodium hyaluronate not only solves the problem of single-function eye drops but also achieves comprehensive conditioning of the ocular surface microenvironment. 2. This invention uses recombinant type III humanized collagen, whose amino acid sequence is highly consistent with human collagen, completely avoiding the potential risks of animal-derived viruses and immune rejection reactions of foreign proteins. It is safer for long-term use and is especially suitable for people with ocular surface sensitivity and those who wear contact lenses for a long time. Detailed Implementation
[0016] This invention provides a collagen-based eye drop containing multiple plant extracts, preferably comprising the following components in the indicated weight percentages: 0.05-0.2% recombinant type III humanized collagen, 0.1-0.3% honeysuckle extract, 0.1-0.25% chrysanthemum extract, 0.03-0.1% salvia miltiorrhiza extract, 0.05-0.12% cassia seed extract, 0.05-0.15% pearl powder extract, 0.03-0.1% rosemary extract, 0.01-0.05% peppermint extract, 0.08-0.2% wolfberry extract, 0.1-0.3% sodium hyaluronate, 0.5-1.5% glycerin, 0.05-0.15% dipotassium glycyrrhizate, and polysorbate 80. 0.1-0.5%, disodium EDTA 0.01-0.05%, balance being water; further preferably, the components include the following mass percentages: recombinant type III humanized collagen 0.1-0.15%, honeysuckle extract 0.15-0.2%, chrysanthemum extract 0.15-0.2%, salvia miltiorrhiza extract 0.05-0.08%, cassia seed extract 0.08-0.1%, pearl powder extract 0.08-0.1%, rosemary extract 0.05-0.08%, peppermint extract 0.02-0.04%, wolfberry extract 0.1-0.15%, sodium hyaluronate 0.15-0.2%, glycerin 0.8-1.2%, dipotassium glycyrrhizate 0.08-0.12%, polysorbate 80 0.2-0.3%, disodium EDTA 0.02-0.04%, balance being water.
[0017] In this invention, the collagen eye drops containing multiple plant extracts preferably also include an osmotic pressure regulator, the amount of which is such that the osmotic pressure of the collagen eye drops containing multiple plant extracts is 280~320 mOsm / L, preferably 290~310 mOsm / L, and more preferably 300 mOsm / L.
[0018] In this invention, the osmotic pressure regulator preferably includes one or more of sodium chloride, potassium chloride, propylene glycol, and sorbitol.
[0019] In this invention, the collagen eye drops containing multiple plant extracts preferably also include a pH adjuster, and the amount of the pH adjuster is such that the pH of the collagen eye drops containing multiple plant extracts is 6.5 to 7.5, preferably 6.8 to 7.2, and more preferably 7.0.
[0020] In this invention, the pH adjuster preferably includes boric acid and / or disodium phosphate.
[0021] This invention also provides a method for preparing the above-mentioned collagen eye drops containing multiple plant extracts, comprising the following steps: 1) Mix sodium hyaluronate, glycerin, and the first portion of water to obtain mixture A; 2) Mix honeysuckle extract, chrysanthemum extract, salvia miltiorrhiza extract, cassia seed extract, pearl powder extract, rosemary extract, peppermint extract, wolfberry extract, dipotassium glycyrrhizate, polysorbate 80, disodium EDTA and water to obtain mixture B; 3) Mix recombinant type III humanized collagen, mixture A, mixture B, osmotic pressure regulator, pH regulator and water (part 3) to obtain collagen eye drops containing multiple plant extracts; There is no requirement for the order of steps 1) and 2).
[0022] In this invention, the mass ratio of the first part of water, the second part of water, and the third part of water is 1~2:4~6:1~2, preferably 1.2~1.8:4.2~5.8:1.2~1.8, more preferably 1.3~1.6:4.3~4.6:1.3~1.6, and even more preferably 1.5:5:1.5.
[0023] In this invention, the mixing temperature in step 1) is 60~90°C, preferably 65~88°C, more preferably 70~85°C, and even more preferably 75~80°C; mixing at this temperature helps with dispersion and dissolution; the mixing temperatures in steps 2) and 3) are independently 20~30°C, preferably 22~28°C, and even more preferably 25°C; this temperature can prevent the degradation and high-temperature denaturation of heat-sensitive components.
[0024] In this invention, the mixing described in step 2) preferably includes a first filtration operation; the first filtration is specifically filtration with a 0.45 μm filter membrane to remove impurities and undissolved substances.
[0025] In this invention, the mixing process described in step 3) preferably includes a second filtration and aseptic filling operation; the second filtration specifically involves filtering with a 0.22 μm filter membrane to remove bacteria and microorganisms, thereby making the eye drops sterile.
[0026] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.
[0027] Example 1
[0028] This embodiment of the collagen eye drops containing multiple plant extracts comprises the following components by weight percentage: 0.05% recombinant type III humanized collagen, 0.1% honeysuckle extract, 0.1% chrysanthemum extract, 0.03% salvia miltiorrhiza extract, 0.05% cassia seed extract, 0.05% pearl powder extract, 0.03% rosemary extract, 0.01% peppermint extract, 0.08% wolfberry extract, 0.1% sodium hyaluronate, 0.5% glycerin, 0.05% dipotassium glycyrrhizate, 0.1% polysorbate 80, 0.01% disodium EDTA, and the balance being water; The preparation method of the above-mentioned collagen eye drops containing multiple plant extracts is as follows: 1) Sodium hyaluronate, glycerin, and water (part 1) are mixed at 60°C according to the above mass percentages to obtain mixture A; 2) The following extracts were mixed at 20°C according to the above mass percentages: honeysuckle extract, cassia seed extract, rosemary extract, peppermint extract, and wolfberry extract purchased from Shaanxi Jiuyuan Biotechnology Co., Ltd.; chrysanthemum extract and salvia miltiorrhiza extract purchased from Shaanxi Wanyuan Biotechnology Co., Ltd.; pearl powder extract (purchased from Shaanxi Snowt Biotechnology Co., Ltd.); dipotassium glycyrrhizate; polysorbate 80; disodium EDTA; and water in the second part. The mixture was then filtered through a 0.45 μm filter membrane to obtain mixture B. 3) Recombinant type III humanized collagen (purchased from Jiangsu Chuangjian Medical Technology Co., Ltd.), mixture A, mixture B, sodium chloride (osmotic pressure regulator), disodium phosphate (pH value regulator), and water (the mass ratio of water in the first, second, and third parts is 1:6:1) were mixed at 20°C, filtered through a 0.22 μm filter membrane, and aseptically filled to obtain collagen eye drops containing multiple plant extracts with an osmotic pressure of 280 mOsm / L and a pH value of 6.5.
[0029] Example 2
[0030] This embodiment of the collagen eye drops containing multiple plant extracts comprises the following components by weight percentage: 0.1% recombinant type III humanized collagen, 0.2% honeysuckle extract, 0.15% chrysanthemum extract, 0.05% salvia miltiorrhiza extract, 0.1% cassia seed extract, 0.1% pearl powder extract, 0.05% rosemary extract, 0.03% peppermint extract, 0.15% wolfberry extract, 0.2% sodium hyaluronate, 0.8% glycerin, 0.08% dipotassium glycyrrhizate, 0.3% polysorbate 80, 0.02% disodium EDTA, and the balance being water; The preparation method of the above-mentioned collagen eye drops containing multiple plant extracts is as follows: 1) Sodium hyaluronate, glycerin, and water (part 1) are mixed at 80°C according to the above mass percentages to obtain mixture A; 2) The honeysuckle extract, chrysanthemum extract, salvia miltiorrhiza extract, cassia seed extract, pearl powder extract, rosemary extract, peppermint extract, wolfberry extract, dipotassium glycyrrhizate, polysorbate 80, disodium EDTA and water in the above mass percentages are mixed at 25°C and then filtered through a 0.45 μm filter membrane to obtain mixture B. 3) Recombinant type III humanized collagen (same as in Example 1), mixture A, mixture B, potassium chloride (osmotic pressure regulator), disodium phosphate (pH value regulator), and water (the mass ratio of water in the first, second, and third parts is 1.5:5:1.5) were mixed at 25°C, filtered through a 0.22 μm filter membrane, and aseptically filled to obtain a collagen eye drop containing multiple plant extracts with an osmotic pressure of 300 mOsm / L and a pH of 7.0.
[0031] Example 3
[0032] This embodiment of the collagen eye drop containing multiple plant extracts comprises the following components by weight percentage: 0.2% recombinant type III humanized collagen, 0.3% honeysuckle extract, 0.25% chrysanthemum extract, 0.1% salvia miltiorrhiza extract, 0.12% cassia seed extract, 0.15% pearl powder extract, 0.1% rosemary extract, 0.05% peppermint extract, 0.2% wolfberry extract, 0.3% sodium hyaluronate, 1.5% glycerin, 0.15% dipotassium glycyrrhizate, 0.5% polysorbate 80, 0.05% disodium EDTA, and the balance being water; The preparation method of the above-mentioned collagen eye drops containing multiple plant extracts is as follows: 1) Sodium hyaluronate, glycerin, and water (part 1) are mixed at 90°C according to the above mass percentages to obtain mixture A; 2) The honeysuckle extract, chrysanthemum extract, salvia miltiorrhiza extract, cassia seed extract, pearl powder extract, rosemary extract, peppermint extract, wolfberry extract, dipotassium glycyrrhizate, polysorbate 80, disodium EDTA and water in the above mass percentages are mixed at 30°C and then filtered through a 0.45 μm filter membrane to obtain mixture B. 3) Recombinant type III humanized collagen (same as in Example 1), mixture A, mixture B, osmotic pressure regulator sorbitol, pH value regulator boric acid, and third part water (the mass ratio of first part water, second part water, and third part water is 2:4:2) were mixed at 25°C, then filtered through a 0.22 μm filter membrane and aseptically filled to obtain collagen eye drops containing multiple plant extracts with an osmotic pressure of 320 mOsm / L and a pH value of 7.0.
[0033] Comparative Example 1
[0034] The only difference between this comparative example and Example 1 is that recombinant type III humanized collagen was not added; all other components and preparation conditions remained the same.
[0035] Comparative Example 2
[0036] The only difference between this comparative example and Example 2 is that the recombinant type III humanized collagen is replaced with animal-derived collagen extracted from rat tail; all other components and preparation conditions remain unchanged.
[0037] Comparative Example 3
[0038] The only difference between this comparative example and Example 3 is that pearl powder extract, rosemary extract, peppermint extract, and wolfberry extract were not added; all other components and preparation conditions remained the same.
[0039] Comparative Example 4
[0040] The only difference between this comparative example and Example 3 is the composition ratio of the collagen eye drops containing multiple plant extracts. Specifically, it includes the following components by mass percentage: 0.2% recombinant type III humanized collagen, 0.05% honeysuckle extract, 0.5% chrysanthemum extract, 0.2% salvia miltiorrhiza extract, 0.3% cassia seed extract, 0.06% pearl powder extract, 0.3% rosemary extract, 0.1% peppermint extract, 0.05% wolfberry extract, 0.3% sodium hyaluronate, 1.5% glycerin, 0.15% dipotassium glycyrrhizate, 0.5% polysorbate 80, 0.05% disodium EDTA, and the balance being water. All other preparation conditions remain unchanged.
[0041] Product efficacy verification: 350 local volunteers were recruited to use the collagen eye drops containing multiple plant extracts prepared according to this invention. The volunteers were long-term users of eye drops that caused eye fatigue and discomfort, aged 21-45 years. The drops were used twice a day, once in the morning and once in the evening, with 3 drops applied each time and left to stand for 1 minute. The treatment was continued for 15 days. The effect of the treatment was evaluated. "Cure" means that the eye discomfort was completely relieved, "improvement" means that the eye discomfort was relieved in a short time, and "ineffective" means that there was no effect after use. The cure rate was the percentage of cured and improved individuals in the total number of participants in a single experimental group. The statistical results are shown in Table 1.
[0042] Table 1. Effects of Collagen Eye Drops Containing Multiple Plant Extracts on Eye Fatigue
[0043] As shown in Table 1, the collagen eye drops containing multiple plant extracts provided by this invention exhibit excellent efficacy in relieving eye fatigue and discomfort: the cure rates of Examples 1-3 reached 96%, 96%, and 98%, respectively, indicating that the eye discomfort symptoms (such as dryness, fatigue, and foreign body sensation) of the vast majority of subjects were significantly improved or completely relieved after 15 days of use. The cure rate of Comparative Example 1, which did not contain recombinant type III humanized collagen, was only 40%, indicating that recombinant type III humanized collagen plays a key role in repairing the ocular surface and promoting comfort. The cure rate of Comparative Example 2, which contained animal-derived collagen, was 44%, still far lower than the examples, confirming that animal-derived collagen has high immunogenicity and poor biocompatibility, leading to a decrease in the effectiveness of the eye drops. The cure rate of Comparative Example 3, which lacked plant extracts such as pearl powder, rosemary, peppermint, and goji berries, was 46%, indicating that the synergistic combination of multiple plant extracts is indispensable for comprehensively relieving eye discomfort. The cure rate of Comparative Example 4, which involved an unreasonable ratio of multiple plant extracts, was only 42%, further illustrating that the synergistic effect of each component within a specific mass percentage range is crucial, and deviations from this range significantly weaken the efficacy. In summary, this invention, through the precise combination of "recombinant humanized collagen + multiple plant extracts," achieves multi-target conditioning of the ocular surface, effectively relieving eye fatigue and discomfort.
[0044] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.
Claims
1. A collagen-based eye drop containing multiple plant extracts, characterized in that, The components include the following mass percentages: Recombinant type III humanized collagen 0.05-0.2%, honeysuckle extract 0.1-0.3%, chrysanthemum extract 0.1-0.25%, salvia miltiorrhiza extract 0.03-0.1%, cassia seed extract 0.05-0.12%, pearl powder extract 0.05-0.15%, rosemary extract 0.03-0.1%, peppermint extract 0.01-0.05%, wolfberry extract 0.08-0.2%, sodium hyaluronate 0.1-0.3%, glycerin 0.5-1.5%, dipotassium glycyrrhizate 0.05-0.15%, polysorbate 80 0.1-0.5%, disodium EDTA 0.01-0.05%, balance water.
2. The collagen eye drop containing multiple plant extracts according to claim 1, characterized in that, It also includes an osmotic pressure regulator, the amount of which is such that the osmotic pressure of the collagen eye drops containing multiple plant extracts is 280~320 mOsm / L.
3. The collagen eye drop containing multiple plant extracts according to claim 2, characterized in that, The osmotic pressure regulator includes one or more of sodium chloride, potassium chloride, propylene glycol, and sorbitol.
4. A collagen-containing eye drop containing multiple plant extracts according to any one of claims 1 to 3, characterized in that, It also includes a pH adjuster, the amount of which is such that the pH of the collagen eye drops containing multiple plant extracts is 6.5 to 7.
5.
5. The collagen eye drop containing multiple plant extracts according to claim 4, characterized in that, The pH adjuster includes boric acid and / or disodium phosphate.
6. A method for preparing a collagen eye drop containing multiple plant extracts as described in any one of claims 1 to 5, characterized in that, Includes the following steps: 1) Mix sodium hyaluronate, glycerin, and the first portion of water to obtain mixture A; 2) Mix honeysuckle extract, chrysanthemum extract, salvia miltiorrhiza extract, cassia seed extract, pearl powder extract, rosemary extract, peppermint extract, wolfberry extract, dipotassium glycyrrhizate, polysorbate 80, disodium EDTA and water to obtain mixture B; 3) Mix recombinant type III humanized collagen, mixture A, mixture B, osmotic pressure regulator, pH regulator and water (part 3) to obtain collagen eye drops containing multiple plant extracts; There is no requirement for the order of steps 1) and 2).
7. The method for preparing a collagen-containing eye drop with multiple plant extracts according to claim 6, characterized in that, The mass ratio of the first part of water, the second part of water, and the third part of water is 1~2:4~6:1~2.
8. The method for preparing a collagen-containing eye drop with multiple plant extracts according to claim 7, characterized in that, The mixing temperature in step 1) is 60~90℃; The mixing temperature described in steps 2) and 3) is 20~30℃.