一种抗原表位多肽及其制备的牙鲆黏蛋白Muc5b特异性抗体
By screening for Muc5b antigenic epitopes in turbot using bioinformatics and preparing antibodies, the problem of specific identification in the study of fish mucosal immune defense mechanisms has been solved. This enables accurate identification and detection of Muc5b in fish mucosal tissues, supporting fish health monitoring and disease diagnosis.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- OCEAN UNIV OF CHINA
- Filing Date
- 2026-04-29
- Publication Date
- 2026-07-17
AI Technical Summary
The lack of antibodies that specifically recognize fish Muc5b in current technologies has hindered the research progress on fish mucosal immune defense mechanisms and made it difficult to deeply understand their role in disease development.
Antigenic epitopes of turbot Muc5b were screened using bioinformatics analysis. After preparation and conjugation with a vector, New Zealand white rabbits were immunized to obtain turbot Muc5b-specific antibodies. Corresponding detection kits were developed, including antigen retrieval solution, blocking solution, labeled antibody and nuclear dye, for the identification and localization of Muc5b-positive mucinous cells.
An antibody that can specifically label Muc5b and positive mucus cells in the mucosal tissue of turbot has been successfully prepared, providing a key detection tool for assessing mucus cell density and distribution, evaluating the relationship between mucus secretion and fish health, and supporting research on fish mucosal immunity and disease diagnosis.
Smart Images
Figure CN122103301B_ABST