一种靶向降解应激颗粒的融合蛋白及其应用
By targeting and degrading stress granules with a fusion protein, the precise identification and degradation of stress granules is achieved using the fusion protein of G3BP1 and TRIM21, which solves the problem of non-specific regulation of stress granules in existing technologies and provides a potential therapeutic strategy for neurodegenerative diseases.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- SUZHOU UNIV
- Filing Date
- 2026-04-28
- Publication Date
- 2026-07-17
AI Technical Summary
Existing technologies struggle to achieve precise regulation of stress particles, especially in neurodegenerative diseases. Current methods cannot distinguish whether the target protein is in an aggregated or free state, leading to non-specific degradation. There is a lack of tools for precise regulation of the stress particle aggregation process.
A fusion protein targeting the degradation of stress particles was constructed, using G3BP1 protein as a recognition module and the RING domain of TRIM21 protein as an induction degradation module to achieve specific degradation of stress particle aggregation. The time sequence was controlled by a tetracycline-inducible gene expression system.
It achieves precise identification of stress particles and ubiquitination-driven degradation, has the ability to identify aggregated states, is highly adaptable, applicable to a variety of expression systems, can observe the intervention effect in real time in living cells, and can be quantitatively evaluated through fluorescence signals.
Smart Images
Figure CN122103377B_ABST