Salt-tolerant composite bacterial population, preparation method thereof and application thereof in high-salt oil sludge remediation

Abstract

The application discloses a salt-tolerant composite bacterial community, a preparation method thereof and application of the composite bacterial community in high-salt oil sludge remediation. Halomonas venusta The composite bacterial community comprises salt-tolerant bacteria of Halomonas sp. Acinetobacter calcoaceticus Acinetobacter calcoaceticus Pseudomonas aeruginosa Pseudomonas aeruginosa H. venusta The application utilizes the Ectoine secretion characteristic of DSM 4743 to provide osmotic pressure protection for non-salt-tolerant strains in the composite bacterial community, constructs an Ectoine secretion-transmission-degradation synergistic mechanism, and greatly improves the degradation efficiency of petroleum hydrocarbon in a high-salt environment. Experiments show that the composite bacterial community can achieve a petroleum hydrocarbon degradation rate of 43.12% in a high-salt oil sludge with a salt content of 35 g / L within 7 days. The application is suitable for oil sludge remediation in a high-salt environment such as offshore oil fields and saline-alkali soil petroleum contaminated sites, and has important environmental and economic benefits.

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