A method for analyzing the response of rhizosphere fungal community of amorphophallus konjac based on exogenous selenium regulation and an information collection method

By designing selenium treatment and control group experiments, and combining fungal-specific amplicon sequencing and ecological analysis, the problem of difficulty in assessing the changes in fungal community structure caused by exogenous selenium application in existing technologies has been solved. This has enabled a systematic analysis of the rhizosphere fungal community of Amorphophallus bulbifera, improved the richness and diversity of the fungal community, and provided a scientific basis for the utilization of selenium fertilizer in agriculture.

CN122104879APending Publication Date: 2026-05-29KUNMING UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
KUNMING UNIVERSITY
Filing Date
2026-03-04
Publication Date
2026-05-29

AI Technical Summary

Technical Problem

Existing technologies lack standardized methods to directly link selenium application intensity with changes in crop rhizosphere fungal community structure, making it difficult to accurately assess the impact of exogenous selenium application on fungal community structure and diversity. Consequently, statistically significant fungal community response patterns cannot be obtained under different selenium application conditions.

Method used

Selenium-treated and control group experiments were designed, and rhizosphere soil samples of Amorphophallus bulbifera were collected. Through fungal-specific amplicon sequencing and ecological index analysis, a method for analyzing the response of Amorphophallus bulbifera rhizosphere fungal community based on exogenous selenium regulation was constructed. This method included soil microbial DNA extraction, amplicon sequencing, and diversity index calculation. Combined with principal coordinate analysis and cluster analysis, changes in fungal community structure were assessed.

Benefits of technology

This study achieved a systematic analysis of the effects of exogenous selenium application on the structure and diversity of the rhizosphere fungal community of Amorphophallus bulbifera, enhancing the richness and diversity of the fungal community, promoting beneficial changes in the rhizosphere microecological environment of Amorphophallus bulbifera, and providing a scientific basis for the utilization of selenium fertilizer in agriculture and soil ecological management.

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Abstract

The application belongs to the field of soil micro-ecological environment, and discloses a kind of based on exogenous selenium regulation's Amorphophallus konjac rhizosphere fungal community response analysis method and system, the method includes: design selenium treatment group and control group to carry out test, sample collection;Soil microorganism DNA extraction and amplicon sequencing;Microbiome under machine data quality control and analysis. With the help of Illumina MiSeq high-throughput sequencing technology, the change of fungal community composition and diversity in the rhizosphere soil of Amorphophallus konjac after the exogenous selenium fertilizer is analyzed, after the exogenous selenium fertilizer is applied, the relative abundance of beneficial microorganisms such as Mortierella, Penicillium and Trichoderma in the rhizosphere soil of Amorphophallus konjac shows an increasing trend, while the relative abundance of potential plant pathogens such as Sclerotium and Fusarium shows a decreasing trend. The above results show that the exogenous selenium fertilizer applied to the soil helps to improve the fungal community diversity and stability in the rhizosphere of Amorphophallus konjac and promote the beneficial transformation of the micro-ecological environment in the rhizosphere of Amorphophallus konjac.
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