An artemisia high-purity extract-based toothpaste and a preparation method thereof

Abstract

The application belongs to the technical field of wormwood toothpaste, and specifically provides a toothpaste based on wormwood high-purity extract, which comprises the following components: a toothpaste base and loaded microspheres; the loaded microspheres are a core of a coprecipitate composite and adherent micro-nano particles and a shell of chitosan gel; the coprecipitate composite comprises wormwood high-purity extract freeze-dried powder, honeysuckle extract and licorice extract; and the adherent micro-nano particles comprise papain and lysozyme. The toothpaste prepared by the application has the advantages of inhibiting plaque bacteria, being sensitive and mild.

Description

Technical Field

[0001] This application belongs to the field of artemisia toothpaste technology, and in particular relates to a toothpaste based on high-purity artemisia extract and its preparation method. Background Technology

[0002] With increasing health awareness, oral care products containing natural herbal active ingredients are gaining popularity among consumers. Extracts from plants such as mugwort, honeysuckle, and licorice have been proven to possess multiple benefits, including antibacterial, anti-inflammatory, and antioxidant properties. Furthermore, enzymes such as lysozyme and papain, due to their specific biological activities, are also being explored for addition to oral care products to enhance cleaning and care effects.

[0003] However, combining multiple active ingredients into a single product system faces significant technical challenges: First, there is the issue of compatibility and stability. The phenolic hydroxyl structure of plant polyphenols easily interacts with amino and thiol groups in proteins, leading to co-precipitation, oxidative inactivation, or covalent bonding and loss of activity. Conventional toothpaste base ingredients, such as anionic surfactants, can also denature proteins. Second, there is the issue of disordered release. Existing technologies mostly employ physical mixing or simple encapsulation, resulting in random and rapid release of each component in the oral cavity. This does not adhere to the ideal sequence of action in oral care, significantly reducing synergistic effects and potentially interfering with healthy oral flora. Third, there is the issue of targeted targeting. Most components lack the ability to actively identify sites of oral inflammation, have short residence times in the dynamic oral environment, and exhibit low bioavailability.

[0004] Therefore, how to improve the application of active ingredients such as mugwort in toothpaste products remains a long-term technical problem that needs to be addressed in this field. Summary of the Invention

[0005] To address the aforementioned issues and further enhance the performance of active ingredients such as Artemisia argyi in toothpaste products, this application provides a toothpaste based on a high-purity extract of Artemisia argyi and its preparation method.

[0006] This application first provides a toothpaste based on a high-purity extract of Artemisia argyi, comprising the following components: a toothpaste matrix and loaded microspheres; wherein the loaded microspheres are composed of a co-precipitated complex and adhesive micro / nano particles as the core and chitosan gel as the shell; The coprecipitation complex includes freeze-dried powder of high-purity Artemisia argyi extract, Lonicera japonica extract, and Glycyrrhiza uralensis extract; The adhesive micro / nanoparticles include papain and lysozyme.

[0007] Furthermore, the mass ratio of the toothpaste matrix to the loaded microspheres is 1:0.02-0.035.

[0008] Furthermore, the toothpaste base comprises the following components in parts by weight: 15-25 parts water, 30-50 parts calcium carbonate, 20-40 parts humectant, 0.2-0.5 parts sodium carboxymethyl cellulose, 0.5-0.8 parts xanthan gum, 0.2-0.5 parts sodium pyrophosphate, 1-1.2 parts fragrance, 2-2.5 parts SDS, 0.2-0.3 parts sodium saccharin, 0.01-0.02 parts sodium hydroxide, and 0.05-0.06 parts methylparaben.

[0009] Furthermore, the moisturizer is at least one of glycerin, sorbitol, and propylene glycol.

[0010] This application provides a method for preparing toothpaste based on high-purity Artemisia argyi extract, comprising the following steps: 1) Sodium alginate and whey protein isolate were dissolved in deionized water to obtain a base solution. Artemisia argyi extract, Lonicera japonica extract and Glycyrrhiza uralensis extract were added to the base solution and homogenized by ultrasonication to obtain a mixture. The mixture was slowly dripped into glyceryl caprylate and emulsified by high-speed shearing. Then it was transferred to a curing solution for curing. After filtration and washing, a coprecipitated complex was obtained. 2) Papain and lysozyme were added to PBS buffer, heated to 55°C, and soybean lecithin was added. The mixture was shaken and extruded through a membrane to obtain liposome microspheres. The liposome microspheres were then dispersed in MES buffer, and lipoic acid and a catalyst were added. The mixture was reacted at room temperature to obtain adhesive micro / nanoparticles. 3) Disperse the coprecipitated complex and the adhered micro / nanoparticles in deionized water, then add chitosan solution, mix well and centrifuge. After centrifugation, take the centrifuged product and redisperse it in deionized water, then add enzymatically hydrolyzed gelatin solution. Repeat the operation, filter, wash, freeze dry to obtain loaded microspheres, and then mix toothpaste matrix and loaded microspheres evenly to obtain the final product.

[0011] Furthermore, in step 1), the mass ratio of the high-purity Artemisia argyi extract, Lonicera japonica extract, and Glycyrrhiza uralensis extract is 1:0.2-0.3:0.5-0.65.

[0012] Furthermore, in step 1), the curing solution is a 0.1M calcium chloride solution.

[0013] Furthermore, in step 2), the mass ratio of papain to lysozyme is 1:1-1.5.

[0014] Furthermore, in step 2), the mass ratio of soybean lecithin to lipoic acid is 1:1.2-1.5.

[0015] Furthermore, in step 3), the mass ratio of the coprecipitated complex to the adhered micro / nano particles is 1:0.35-0.5.

[0016] Compared with the prior art, this application has the following beneficial effects: This application employs a multi-layered composite structure, constructing a chitosan mineralization layer on the outermost layer to provide a dense physical barrier. This effectively blocks the penetration of inactivating factors such as oxygen and metal ions, slows down the oxidative hydrolysis of polyphenols, and protects the internal co-precipitated complex and adherent micro / nano particles. Furthermore, isolation is maintained between the co-precipitated complex and the adherent micro / nano particles, allowing for the sequential release of different active components, maximizing their effectiveness, and reducing interference between components. Additionally, pH-triggered adhesion and enzyme-triggered deshielding release the internal components, disrupting the biofilm of harmful bacteria such as dental plaque, inhibiting plaque formation, and reducing plaque buildup. Moreover, the released papain and mugwort active ingredients can alleviate the irritation to teeth caused by hot, cold, sweet, and sour foods, relieving tooth sensitivity and exhibiting excellent anti-sensitivity effects. Attached Figure Description

[0017] Figure 1 This is a graph showing the anti-allergy performance test data of the toothpaste in Example 2 of this application and commercially available toothpaste products.

[0018] Figure 2 This is a graph showing the antibacterial performance test data of the toothpaste in Example 2 of this application and commercially available toothpaste products.

[0019] Figure 3 This is a schematic diagram showing the release data of the loaded microspheres in Examples 1-2 and the control group of this application. Detailed Implementation

[0020] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of this application.

[0021] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0022] When using “including,” “having,” and “contains” as described herein, the intention is to cover non-exclusive inclusion, unless an explicit qualifying term such as “only,” “consisting of,” etc., is used, in which case another component may be added.

[0023] The terms "preferred," "more preferably," "better," and "even better" used in this application refer to embodiments of this application that provide certain beneficial effects under certain circumstances. However, other embodiments may also be preferred under the same or other circumstances. Furthermore, the description of one or more preferred embodiments does not imply that other embodiments are unavailable, nor is it intended to exclude other embodiments from the scope of this application. That is, in this application, "preferred," "more preferably," "better," and "even better" are merely descriptions of implementations or embodiments with better effects, but do not constitute a limitation on the scope of protection of this application.

[0024] In this application, terms such as "further," "even more," and "particularly" are used for descriptive purposes and indicate differences in content, but should not be construed as limiting the scope of protection of this application.

[0025] In this application, "at least one" means one or more, such as one, two, or more. "Multiple" or "several" means at least two, such as two, three, etc., and "multi-layered" means at least two layers, such as two layers, three layers, etc., unless otherwise explicitly specified. In the description of this application, "several" means at least one, such as one, two, etc., unless otherwise explicitly specified.

[0026] When a numerical range is disclosed herein, the range is considered continuous and includes the minimum and maximum values ​​of the range, as well as every value between the minimum and maximum values. Furthermore, when the range refers to integers, it includes every integer between the minimum and maximum values ​​of the range. Additionally, when multiple ranges are provided to describe a feature or characteristic, the ranges may be combined. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges to which they are incorporated.

[0027] Unless otherwise specified, all steps in this application may be performed sequentially or randomly. For example, the method comprising steps (a) and (b) indicates that the method may include steps (a) and (b) performed sequentially, or it may include steps (b) and (a) performed sequentially. For example, the mention that the method may also include step (c) indicates that step (c) may be added to the method in any order; for example, the method may include steps (a), (b), and (c), or it may include steps (a), (c), and (b), or it may include steps (c), (a), and (b), etc. Unless otherwise stated, singular terms may include plural forms and should not be construed as having a quantity of one.

[0028] In this application, "above" or "below" includes the number itself. For example, "below 1" includes 1.

[0029] In this application, room temperature refers to 0~40℃, including but not limited to 10~40℃, or further to 20~30℃.

[0030] Based on extensive experimental research, this application provides a toothpaste based on high-purity Artemisia argyi extract, comprising the following components: toothpaste matrix and supported microspheres; wherein the supported microspheres are composed of a co-precipitated complex and adhesive micro / nano particles as the core and chitosan gel as the shell; The coprecipitation complex includes freeze-dried powder of high-purity Artemisia argyi extract, Lonicera japonica extract, and Glycyrrhiza uralensis extract; The adhesive micro / nanoparticles include papain and lysozyme.

[0031] Furthermore, the mass ratio of the toothpaste matrix to the loaded microspheres is 1:0.02-0.035.

[0032] In some specific embodiments, the mass ratio of the toothpaste matrix to the supported microspheres can be 1:0.02, 1:0.021, 1:0.022, 1:0.023, 1:0.024, 1:0.025, 1:0.026, 1:0.027, 1:0.028, 1:0.029, 1:0.030, 1:0.031, 1:0.032, 1:0.033, 1:0.034, or 1:0.035. Generally, a mass ratio of 0.025 or 0.026 for the toothpaste matrix to the supported microspheres yields better experimental results.

[0033] Furthermore, the toothpaste base comprises the following components in parts by weight: 15-25 parts water, 30-50 parts calcium carbonate, 20-40 parts humectant, 0.2-0.5 parts sodium carboxymethyl cellulose, 0.5-0.8 parts xanthan gum, 0.2-0.5 parts sodium pyrophosphate, 1-1.2 parts fragrance, 2-2.5 parts SDS, 0.2-0.3 parts sodium saccharin, 0.01-0.02 parts sodium hydroxide, and 0.05-0.06 parts methylparaben.

[0034] Furthermore, the moisturizer is at least one of glycerin, sorbitol, and propylene glycol.

[0035] This application provides a method for preparing toothpaste based on high-purity Artemisia argyi extract, comprising the following steps: 1) Sodium alginate and whey protein isolate were dissolved in deionized water to obtain a base solution. Artemisia argyi extract, Lonicera japonica extract and Glycyrrhiza uralensis extract were added to the base solution and homogenized by ultrasonication to obtain a mixture. The mixture was slowly dripped into glyceryl caprylate and emulsified by high-speed shearing. Then it was transferred to a curing solution for curing. After filtration and washing, a coprecipitated complex was obtained. 2) Papain and lysozyme were added to PBS buffer, heated to 55°C, and soybean lecithin was added. The mixture was shaken and extruded through a membrane to obtain liposome microspheres. The liposome microspheres were then dispersed in MES buffer, and lipoic acid and a catalyst were added. The mixture was reacted at room temperature to obtain adhesive micro / nanoparticles. 3) Disperse the coprecipitated complex and the adhered micro / nanoparticles in deionized water, then add chitosan solution, mix well and centrifuge. After centrifugation, take the centrifuged product and redisperse it in deionized water, then add enzymatically hydrolyzed gelatin solution. Repeat the operation, filter, wash, freeze dry to obtain loaded microspheres, and then mix toothpaste matrix and loaded microspheres evenly to obtain the final product.

[0036] Furthermore, in step 1), the mass ratio of the high-purity Artemisia argyi extract, Lonicera japonica extract, and Glycyrrhiza uralensis extract is 1:0.2-0.3:0.5-0.65.

[0037] In some specific embodiments, in step 1), the mass ratio of the high-purity Artemisia argyi extract, Lonicera japonica extract, and Glycyrrhiza uralensis extract can be 1:0.2:0.5, 1:0.21:0.5, 1:0.21:0.5, 1:0.23:0.5, 1:0.24:0.5, 1:0.25:0.5, 1:0.26:0.5, 1:0.27:0.5, 1:0.28:0.5, 1:0.29:0.5, 1:0.3:0.5, or 1:0.2: The following ratios are used: 0.51, 1:0.21:0.52, 1:0.21:0.53, 1:0.23:0.54, 1:0.24:0.55, 1:0.25:0.56, 1:0.26:0.57, 1:0.27:0.58, 1:0.28:0.59, 1:0.29:0.6, 1:0.3:0.61, 1:0.2:0.62, 1:0.21:0.63, 1:0.21:0.64, 1:0.23:0.55. Under normal circumstances, in step 1), a mass ratio of 1:0.25:0.5 for the high-purity extract of Artemisia argyi, the extract of Lonicera japonica, and the extract of Glycyrrhiza uralensis yields better experimental results.

[0038] Furthermore, in step 1), the curing solution is a 0.1M calcium chloride solution.

[0039] Furthermore, in step 2), the mass ratio of papain to lysozyme is 1:1-1.5.

[0040] In some specific embodiments, in step 2), the mass ratio of papain to lysozyme can be 1:1, 1:1.2, 1:1.3, 1:1.4, or 1:1.5.

[0041] Furthermore, in step 2), the mass ratio of soybean lecithin to lipoic acid is 1:1.2-1.5.

[0042] In some specific embodiments, in step 2), the mass ratio of soybean lecithin to lipoic acid can be 1:1.2, 1:1.3, 1:1.4, or 1:1.5. Generally, a mass ratio of 1:1.2 or 1:1.25 for soybean lecithin to lipoic acid yields better experimental results.

[0043] Furthermore, in step 3), the mass ratio of the coprecipitated complex to the adhered micro / nano particles is 1:0.35-0.5.

[0044] In some specific implementations, in step 3), the mass ratio of the coprecipitated complex to the adhered micro / nano particles is 1:0.35, 1:0.36, 1:0.37, 1:0.38, 1:0.39, 1:0.40, 1:0.41, 1:0.42, 1:0.43, 1:0.44, 1:0.45, 1:0.46, 1:0.47, 1:0.48, 1:0.49, or 1:0.5. Generally, a mass ratio of 1:0.38, 1:0.39, or 1:0.40 yields better results.

[0045] The present application will be further illustrated by the following examples, but these examples do not limit the scope of the present application.

[0046] When numerical ranges are given in the embodiments, it should be understood that, unless otherwise stated in this application, both endpoints of each numerical range and any value between the two endpoints may be selected. Unless otherwise defined, all technical and scientific terms used in this application have the same meaning as commonly understood by one of ordinary skill in the art. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall apply. All reagents or instruments whose manufacturers are not specified are conventional products that can be purchased commercially. In addition to the specific methods, equipment, and materials used in the embodiments, based on the knowledge of the prior art possessed by one of ordinary skill in the art and the description in this application, any prior art methods, equipment, and materials similar to or equivalent to those described, used, or made by the methods, equipment, and materials in the embodiments of this application may be used to implement this application.

[0047] Example 1 The toothpaste based on high-purity Artemisia argyi extract in this embodiment includes the following components by weight: 1g loaded microspheres and 39g toothpaste matrix.

[0048] The toothpaste base is composed of the following components by weight: 20g water, 45g calcium carbonate, 15g glycerin, 25g sorbitol, 0.3g sodium carboxymethyl cellulose, 0.6g xanthan gum, 0.35g sodium pyrophosphate, 1g fragrance, 2.5g SDS, 0.25g sodium saccharin, 0.015g sodium hydroxide, and 0.055g methylparaben.

[0049] The toothpaste preparation method based on high-purity Artemisia argyi extract in this embodiment includes the following steps: 1) Dissolve 2g sodium alginate and 0.8g whey protein isolate in 50mL of deionized water and stir until homogeneous to obtain a base solution. Add 200mg of Artemisia argyi extract, 50mg of Lonicera japonica extract and 100mg of Glycyrrhiza uralensis extract to the base solution and homogenize by sonication to obtain a mixture. Slowly drop the mixture into 100mL of caprylic / capric triglyceride at a rate of 2mL / min and emulsify at 10000rpm for 3min to obtain an emulsion. Prepare a 0.15M calcium chloride solution as a curing bath, and then transfer the obtained emulsion into the curing bath through a 0.5mm diameter injection needle and cure for 30min. Filter, wash and dry to obtain a coprecipitated complex. 2) Add 1g of papain and 1.5g of lysozyme to 100mL of PBS buffer and mix well. After heating to 55℃, add 5g of soybean lecithin, shake, and extrude the membrane to obtain liposome microspheres. Then disperse the liposome microspheres in MES buffer, add 6g of lipoic acid and EDC catalyst, and react at room temperature in the dark for 3h to obtain adhesive micro-nano particles. 3) Disperse 1g of coprecipitated complex and 0.75g of adhesive micro / nanoparticles into 200mL of deionized water, then add 500mL of chitosan solution, mix well and centrifuge. After centrifugation, take the centrifuged product and redisperse it into deionized water, then add 500mL of enzymatically hydrolyzed gelatin solution. Repeat the operation three times, filter, wash, freeze dry to obtain loaded microspheres, and then mix toothpaste matrix and loaded microspheres evenly to obtain the final product.

[0050] The chitosan solution preparation method of this embodiment is as follows: dissolve 2g of chitosan with a deacetamide degree ≥95% in 100mL of 1% (v / v) acetic acid aqueous solution and stir overnight.

[0051] The method for preparing the enzymatic hydrolyzed gelatin solution in this embodiment is as follows: Take 1g of enzymatic hydrolyzed gelatin with an average molecular weight of 3000-5000 Da and a Bloom strength of 150-250, dissolve it in 100mL of deionized water at 60℃, and stir until completely clear.

[0052] Example 2 The toothpaste based on high-purity Artemisia argyi extract in this embodiment includes the following components by weight: 1g loaded microspheres and 39g toothpaste matrix.

[0053] The toothpaste base is composed of the following components by weight: 20g water, 45g calcium carbonate, 15g glycerin, 25g sorbitol, 0.3g sodium carboxymethyl cellulose, 0.6g xanthan gum, 0.35g sodium pyrophosphate, 1g fragrance, 2.5g SDS, 0.25g sodium saccharin, 0.015g sodium hydroxide, and 0.055g methylparaben.

[0054] The toothpaste preparation method based on high-purity Artemisia argyi extract in this embodiment includes the following steps: 1) Dissolve 2g sodium alginate and 0.8g whey protein isolate in 50mL of deionized water and stir until homogeneous to obtain a base solution. Add 200mg of Artemisia argyi extract, 50mg of Lonicera japonica extract and 100mg of Glycyrrhiza uralensis extract to the base solution and homogenize by sonication to obtain a mixture. Slowly drop the mixture into 100mL of caprylic / capric triglyceride at a rate of 2mL / min and emulsify by high-speed shearing at 10000rpm for 3min to obtain an emulsion. Prepare a 0.1M calcium chloride solution as a curing bath, and then transfer the obtained emulsion into the curing bath through a 0.5mm diameter injection needle and cure for 30min. Filter, wash and dry to obtain a coprecipitated complex. 2) Add 1g of papain and 1.5g of lysozyme to 100mL of PBS buffer and mix well. After heating to 55℃, add 5g of soybean lecithin, shake, and extrude the membrane to obtain liposome microspheres. Then disperse the liposome microspheres in MES buffer, add 6g of lipoic acid and EDC catalyst, and react at room temperature in the dark for 3h to obtain adhesive micro-nano particles. 3) Disperse 1g of coprecipitated complex and 0.4g of adhesive micro / nanoparticles into 200mL of deionized water, then add 500mL of chitosan solution, mix well and centrifuge. After centrifugation, take the centrifuged product and redisperse it into deionized water, then add 500mL of enzymatically hydrolyzed gelatin solution. Repeat the operation three times, filter, wash, freeze dry to obtain loaded microspheres, and then mix toothpaste matrix and loaded microspheres evenly to obtain the final product.

[0055] The chitosan solution preparation method of this embodiment is as follows: dissolve 1g of chitosan with a deacetamide degree ≥95% in 100mL of 1% (v / v) acetic acid aqueous solution and stir overnight.

[0056] The method for preparing the enzymatic hydrolyzed gelatin solution in this embodiment is as follows: Take 1g of enzymatic hydrolyzed gelatin with an average molecular weight of 3000-5000 Da and a Bloom strength of 150-250, dissolve it in 100mL of deionized water at 60℃, and stir until completely clear.

[0057] control group The preparation method of the loaded microspheres in this control group includes the following steps: 2g sodium alginate and 0.8g whey protein isolate were dissolved in 50mL deionized water and stirred evenly to obtain a base solution. 200mg of Artemisia argyi high-purity extract, 50mg of Lonicera japonica extract, 100mg of Glycyrrhiza uralensis extract, 50mg of papain and 75mg of lysozyme were added to the base solution and homogenized by ultrasonication to obtain a mixture. The mixture was slowly added dropwise to 100mL of caprylic / capric triglyceride at a rate of 2mL / min and emulsified by high-speed shearing at 10000rpm for 3min to obtain an emulsion. A 0.1M calcium chloride solution was prepared as a curing bath. The obtained emulsion was then transferred to the curing bath through an injection needle with a diameter of 0.5mm and cured for 30min. After filtration, washing and drying, the loaded microspheres were obtained.

[0058] Performance testing The toothpaste of Example 2 of this application (referred to as AL in the figure) was compared with commercially available toothpaste products in terms of its anti-allergy and antibacterial effects. The specific test results are as follows: Figure 1-2 As shown.

[0059] Accurately weigh 2g of the loaded microspheres from Examples 1-2 and the control group into a 250mL Erlenmeyer flask, add 50mL of oral environment simulation solution, the components of which include: NaCl 0.4g, KCl 0.4g, NaH2PO4·2H2O 0.78g, CaCl2·2H2O 0.795g, Na2S·2H2O 0.005g, urea 1.0g, and distilled water 1L. Adjust the pH to 5.8 using lactic acid.

[0060] The conical flask was placed in a constant temperature shaker at 37℃ and 120rpm. 1mL of supernatant was collected at 1, 4, 7, 10, 15, 18, 21, 24, 27, and 30 minutes. An equal volume of simulated solution was added after each sampling. Using the high-purity Artemisia argyi extract as a baseline, the release amount at different time points was measured using UV-Vis. The cumulative release rate was calculated as follows: Cumulative release rate % = Cumulative mass of high-purity Artemisia argyi extract released / Total mass of high-purity Artemisia argyi extract loaded in the microspheres. The results are shown below. Figure 3 As shown.

[0061] analyze Figure 1-3 As can be seen, the toothpaste of this application has an anti-sensitivity effect 4.56 times that of the lowest commercially available toothpaste and 1.28 times that of the highest commercially available toothpaste, demonstrating excellent anti-sensitivity performance. The loaded microspheres of this application have a good sustained-release effect. The release rate of control group 1 was too fast, which would irritate the oral cavity, while the release rate of this application is gentle and gradual, with a long duration of action, and is mild and non-irritating.

[0062] Although this application has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A toothpaste based on a high-purity extract of Artemisia argyi, characterized in that: It comprises the following components: toothpaste matrix and supported microspheres; the supported microspheres are composed of a coprecipitated complex and adhesive micro / nano particles as the core and chitosan gel as the shell; The coprecipitation complex includes freeze-dried powder of high-purity Artemisia argyi extract, Lonicera japonica extract, and Glycyrrhiza uralensis extract; The adhesive micro / nanoparticles include papain and lysozyme.

2. The toothpaste based on high-purity Artemisia argyi extract according to claim 1, characterized in that: The mass ratio of the toothpaste matrix to the loaded microspheres is 1:0.02-0.

035.

3. The toothpaste based on high-purity Artemisia argyi extract according to claim 1, characterized in that: The toothpaste base comprises the following components by mass fraction: 15-25 parts water, 30-50 parts calcium carbonate, 20-40 parts humectant, 0.2-0.5 parts sodium carboxymethyl cellulose, 0.5-0.8 parts xanthan gum, 0.2-0.5 parts sodium pyrophosphate, 1-1.2 parts fragrance, 2-2.5 parts SDS, 0.2-0.3 parts sodium saccharin, 0.01-0.02 parts sodium hydroxide, and 0.05-0.06 parts methylparaben.

4. The toothpaste based on high-purity Artemisia argyi extract according to claim 1, characterized in that: The moisturizer is at least one of glycerin, sorbitol, and propylene glycol.

5. A method for preparing toothpaste based on high-purity Artemisia argyi extract as described in claim 1, characterized in that: Includes the following steps: 1) Sodium alginate and whey protein isolate were dissolved in deionized water to obtain a base solution. Artemisia argyi extract, Lonicera japonica extract and Glycyrrhiza uralensis extract were added to the base solution and homogenized by ultrasonication to obtain a mixture. The mixture was slowly dripped into glyceryl caprylate and emulsified by high-speed shearing. Then it was transferred to a curing solution for curing. After filtration and washing, a coprecipitated complex was obtained. 2) Papain and lysozyme were added to PBS buffer, heated to 55°C, and soybean lecithin was added. The mixture was shaken and extruded through a membrane to obtain liposome microspheres. The liposome microspheres were then dispersed in MES buffer, and lipoic acid and a catalyst were added. The mixture was reacted at room temperature to obtain adhesive micro / nanoparticles. 3) Disperse the coprecipitated complex and the adhered micro / nanoparticles in deionized water, then add chitosan solution, mix well and centrifuge. After centrifugation, take the centrifuged product and redisperse it in deionized water, then add enzymatically hydrolyzed gelatin solution. Repeat the operation, filter, wash, freeze dry to obtain loaded microspheres, and then mix toothpaste matrix and loaded microspheres evenly to obtain the final product.

6. The method for preparing toothpaste based on high-purity Artemisia argyi extract according to claim 5, characterized in that: In step 1), the mass ratio of the high-purity extract of Artemisia argyi, the extract of Lonicera japonica, and the extract of Glycyrrhiza uralensis is 1:0.2-0.3:0.5-0.

65.

7. The method for preparing toothpaste based on high-purity Artemisia argyi extract according to claim 5, characterized in that: In step 1), the curing solution is a 0.1M calcium chloride solution.

8. The method for preparing toothpaste based on high-purity Artemisia argyi extract according to claim 5, characterized in that: In step 2), the mass ratio of papain to lysozyme is 1:1-1.

5.

9. The method for preparing toothpaste based on high-purity Artemisia argyi extract according to claim 5, characterized in that: In step 2), the mass ratio of soybean lecithin to lipoic acid is 1:1.2-1.

5.

10. The method for preparing toothpaste based on high-purity Artemisia argyi extract according to claim 5, characterized in that: In step 3), the mass ratio of the coprecipitated complex to the adhered micro / nano particles is 1:0.35-0.5.

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