一种抗原表位多肽及其制备的牙鲆mAChR3特异性抗体
By preparing a specific antibody against turbot mAChR3, the lack of molecular recognition tools for turbot mAChR3 was solved, enabling in-depth research on mucus barrier function and immune enhancement, and promoting the development of products that enhance fish mucosal barrier function and disease-resistant breeding.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- OCEAN UNIV OF CHINA
- Filing Date
- 2026-04-29
- Publication Date
- 2026-07-17
AI Technical Summary
In bony fish such as turbot, there is a lack of specific recognition tools for the mAChR3 molecule, resulting in insufficient research on the regulatory mechanism of mAChR3 in the mucus barrier, which affects the regulation of the mucus barrier and the immune defense capabilities of fish.
To prepare a specific antibody against turbot mAChR3, the B-cell antigenic epitope peptide RASKRSFNTKATTV was screened and conjugated with a KLH vector. Balb/c mice were then immunized with the resulting antibody to prepare a specific antibody that can recognize the mAChR3 molecule. A corresponding immunoassay kit was developed to detect mAChR3-positive cells.
It provides tools for identifying mAChR3 molecules and their positive cells, systematically elucidates the key molecular mechanism by which ACh regulates mucus barrier function through the mAChR3 receptor, advances research on fish mucosal barrier function, enhances fish immunity, and supports the development of products that strengthen mucus barrier function and disease-resistant breeding.
Smart Images
Figure CN122127433B_ABST