一种青霉素酶突变体及其重组菌株与应用
By performing site-directed mutagenesis on penicillinase, its catalytic activity and stability under high alkaline conditions were improved, solving the problem of the rapid decrease in activity of existing penicillinase under high alkaline conditions. This enabled efficient purification treatment of penicillin production wastewater, reducing operating costs and improving treatment efficiency.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- HUNAN INSTITUTE OF ENGINEERING
- Filing Date
- 2026-05-07
- Publication Date
- 2026-07-17
AI Technical Summary
Existing penicillinases exhibit a sharp drop in activity under highly alkaline conditions, making them unsuitable for the wide pH fluctuations in penicillin production wastewater. This results in unstable enzymatic pretreatment effects, high operating costs, and an inability to meet industrial-scale requirements.
By performing site-directed mutagenesis on wild-type penicillinase, its amino acid sequence was modified to improve its catalytic activity and stability in the pH range of 5-10, especially maintaining high enzyme activity under highly alkaline conditions of pH 9-10, thus preparing penicillinase mutants and their recombinant strains.
The penicillinase mutant maintains high catalytic activity over a wide pH range, solving the problem of sudden drop in enzyme activity, simplifying the process, reducing operating costs, adapting to pH fluctuations in wastewater, improving treatment efficiency, and meeting green and environmental protection requirements.
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